... indicating that the thermal
unfolding process ofthe precursor form follows a two-state
transition. The ratio ofthe unfolding enthalpy (DH
cal
)tothe
van’t Hoff enthalpy (DH
v
) was 1.05 (DH
cal
and ... for the baseline.
DH
cal
and DH
v
(van’t Hoff enthalpy) were determined from
the scanned data using the
ORIGIN
software program
(version 4.0) (MicroCal Inc.).
Qualitative thermodynamic parameters
for ... or was obtained from Oriental Yeast Co. (Lot
21003805) (Osaka, Japan) and proCPY was prepared as the
same manner as CPY, with minor modifications. Dgly CPY
and Dgly proCPY, in which the asparagine...
... The Marketing Strategy ofa multinational join stock company
multinational join stock company has a certain advantage: a multinational join stock
company has always kept its prices as competitive ... stock company size and market share have been small. The
shortage of capital is main cause ofthe company’s modest size and market share. Up to
now, a multinational join stock company has had only ...
(American) and Sanyo (Japanese). These are famous brands in the world market and
Vietnamese consumers highly appreciate them. A multinational join stock company has
never had any complaint about the...
... in the child DataTable. This is the default.
None Indicates that no action takes place.
SetDefault Indicates that the DataColumn values in the child DataTable are to be set
to the value in the ... you change the DataColumn in
the parent DataTable on which the ForeignKeyConstraint was created, then the same
change is also made in any corresponding DataRow objects in the child DataTable. ...
UpdateRule is set to None.
ã
The CommandText ofthe Command object in the UpdateCommand ofthe
DataAdapter is the same as in the second case.
The following code sets the UpdateRule of the...
... live in a snake.
14. My brother managed to kill the snake just at the time when I (be) had been almost
exhausted. If he (be) had been a little late, I (kill) would have been killed by the snake.
15. ... buy a car. I wish I (have) had
had enough money to buy a car.
10. I wish our teacher (explain) would explain that lesson to us again tomorrow.
11. I can hear his voice so clearly that it is as if ... hurry) don't hurry
4. Mary would not have got wet if she (wear) had worn a raincoat.
5. If today (be) were a holiday, I would stay in bed for all day long.
6. If she ( make) made him change his...
... phosphorylation. The data presented here suggest that the
pro-domain of BMP-2 can alter the signalling properties ofthe growth factor
by modulating the ability ofthe mature part to interact with the ... was assessed at a single time
point (30 days), and neither the pharmacokinetics of
proBMP-2 nor the kinetics of bone formation were anal-
ysed. Thus, the fate of administered proBMP-2 in the
animal ... can affect both the maturation and functions of mature
growth factors. Here, we assessed the biological function ofthe pro -form of
bone morphogenetic protein-2 (BMP-2), a member ofthe transforming
growth...
... o
AthalianaB
PativumB
SoleraceaB
NtabacumB
A. thalianaA
PsativumA
SoleraceaA
Chlamy
Synechocystis
Synechococcus
AthalianaB
PativumB
SoleraceaB
NtabacumB
A. thalianaA
PsativumA
SoleraceaA
Chlamy
Synechocystis
Synechococcus
AthalianaB
PativumB
SoleraceaB
NtabacumB
A. thalianaA
PsativumA
SoleraceaA
Chlamy
Synechocystis
Synechococcus
119
119
119
119
119
120
119
121
118
119
159
159
159
159
157
158
157
160
158
159
199
199
199
199
197
198
197
200
198
199
VI ... (Fig. 1A) , and that
of chloroplast spinach GAPDH [35] w ere examined t o
AthalianaB
PativumB
SoleraceaB
NtabacumB
A. thalianaA
PsativumA
SoleraceaA
Chlamy
Synechocystis
Synechococcus
AthalianaB
PativumB
SoleraceaB
NtabacumB
A. thalianaA
PsativumA
SoleraceaA
Chlamy
Synechocystis
Synechococcus
AthalianaB
PativumB
SoleraceaB
NtabacumB
A. thalianaA
PsativumA
SoleraceaA
Chlamy
Synechocystis
Synechococcus
119
119
119
119
119
120
119
121
118
119
159
159
159
159
157
158
157
160
158
159
199
199
199
199
197
198
197
200
198
199
VI ... R A R AAA L N I V P T S T G AA K A VAL V L PNL K G K L N G I A L R V P
R R A R AAA L N I V P T S T G AA K A VAL V L PTL K G K L N G I A L R V P
R R A R A ACL N I V P T S T G AA K A VAL...
... link the senses across
dictionaries, hence Wik is only used as augmented
data for WMF to better learn the semantics of words.
All data is tokenized, POS tagged (Toutanova et al.,
2003) and lemmatized, ... (adjectives and adverbs) do not have
a taxonomic representation structure. For example,
the jcn similarity measure (Jiang and Conrath, 1997)
computes the sense pair similarity score based on the
information ... this case, many senses will share the
same latent semantics profile, as long as they are in
the same topic/domain.
To solve the sparsity issue we use missing words
as negative evidence of latent...
... Peplow, F. Yamakura and T. Matsumoto for the
analyses of iron and manganese in protein samples. We also wish to
thank H. Steinman for the gift of E. coli OX32 6A. We finally thank Mr
M. Farrugia for ... incubated at 50 °Cfortheindicated
times. Aliquots were removed for analysis of SOD activity and were
calculated to give 1 unit of SOD activity in thestandard SOD assay
conditions used and all values ... helper phage (Stratagene). One microgram was
utilized in mutagenesis reactions together with oligonucleo-
tides ECF-Q69G d(5Â-AACAACGCAGCT
GGGCTCTG
GAACCAT), ECF -A1 41Q d(5Â-TCAACCTCTAAC
CAG
GCTACTCCGCTG)...
... probably a consequence
of a mistake incorporated by the TaKaRa Ex TaqÔ poly-
merase and did not affect the enzymatic activity.
MS
The molecular mass ofthe recombinant b-fructofuranosi-
dase was ... Technical University of Lodz, Poland
Introduction
Bifidobacteria are found in human and animal gastro-
intestinal tracts, as well as in the oral cavity and the
vagina [1]. They are among the first bacteria ... MS of recombi-
nant b-fructofuranosidase are close to the theoretical
value ofthe molecular mass. A peak of molecular ion
at m ⁄ z = 58 879 Da was observed on the MALDI-
TOF mass spectrum. The...
... regulation of DNA replication initiation
[19]. On the other hand, DNA replication and repair
factors, such as MCM5, BRCA1 and Ku, have been
implicated as positive or negative regulators of tran-
scription ... Overall, the staining intensities of RNA
pol II and Pol e do not correlate, and there is only a
very minor effect of local UV damage on the nuclear
localization of RNA pol II and Pol e.
The RNA ... IIA and the IIO form. Although
less RNA pol II appears to be recognized by the anti-
body in this application, the focal colocalization of
RNA pol II and Pol e remained apparent (Fig. 4C).
Although...
... glycosyl-
transferase elucidate catalysis in the alpha-amylase family. Nat.
Struct. Biol. 6, 432–436.
16. Terwisscha van Scheltinga, A. C., Armand, S., Kalk, K.H., Isogai,
A. , Henrissat, B. & Dijkstra, ... crystallo-
graphy that replacement ofthe Asp142 analogue by alanine
in other family 18 chitinases puts the N-acetyl group in a
conformation which is not favourable for nucleophilic
attack on the ... with partial
charges being formed, and covalent bonds being formed
and broken). Thus, the calculated effect of rotation of
Asp142 on the pK
a
of Glu144 only gives an indication of
what may happen...
... 5Â-CCCCATATGATGAGGACAGATT
CATCAAAAATG-3Â and 5Â-CATGGATCCTCATTTT
TCTTCATTTTGAGGC-3Â. The amplied PCR fragment
was inserted into plasmid vector pET between the endo-
nuclease sites NdeIandHindIII. The ... of Lowry et al. [14] and the purity was examined
by SDS/PAGE [15].
Assay of human PKIb activity
The activity of purified human PKIb was assayed by the
inhibition ofthe catalytic subunit of cAPK ... report the
purification and structural characteristics of human PKIb.
Experimental procedures
Materials
Unless otherwise stated, all reagents were of analytical grade.
The bacterial strain Escherichia...
... structure of an HHMM
The models discussed here are evaluated
by applying them to natural language tasks
based on CoNLL-2004
1
and a sub-corpus
of the Lancaster Treebank
2
.
Keywords: information extraction, ... only the part -of- speech tags and grammar
information are considered. The left hand side of
the figure shows the original structure ofthe sen-
tence, and the right hand side shows the trans-
formed ... Example ofa transformed HHMM with
the internal state S
2
.
The procedure to transform internal states is:
I) calculate the transformed observation (
¯
O) for
each internal state; II) apply the...
... and barley a- amylases are thus also presented.
MATERIALS AND METHODS
SUMA
software: subsite mapping of amylases
This software calculates the apparent binding energies on
the basis ofthe measured ... PPA, an
a- amylase studied by us earlier. Also an attempt has been
made to use this program for subsite mapping of other
a- amylases found in the literature. Evaluations of subsite
maps of rice and ... Mapping of a- Amylases) is freely available
for research and educational purposes via the Internet
(E-mail: gyemant@tigris.klte.hu).
The advantages of this program are demonstrated
through a- amylases...