the standard form of a complex number is

... B=MIC B ị=n where A and B are the MICs of drug A and drug B in the combination, MIC A and MIC B are the MICs of drug A and drug B alone, FIC A and FIC B are the FICs of drug A and drug B and n is the number of ... & Batoni G (2008) Comparative analysis of the bactericidal activities of amphibian peptide analogues against multidrug-resistant nosocomial bacterial strains. Antimicrob Agents Chemother 52, ... thank M. Simmaco for use of the facilities and platforms available in the DiMA Unit of the Sant’An- drea Hospital. This work was funded in part by Italian Ministero dell’Universita ` e Ricerca...

Ngày tải lên: 16/03/2014, 00:20

Ngày tải lên: 07/08/2014, 15:23

... are also made in the child DataTable. This is the default. None Indicates that no action takes place. SetDefault Indicates that the DataColumn values in the child DataTable are to be set to the ... Cascade; therefore, when you change the DataColumn in the parent DataTable on which the ForeignKeyConstraint was created, then the same change is also made in any corresponding DataRow objects in the ... to the database. By default, DeleteRule is set to Cascade; therefore, when you delete a DataRow in the parent DataTable, any corresponding DataRow objects in the child DataTable are also deleted....

Ngày tải lên: 24/12/2013, 01:17

... chloroplast spinach GAPDH [35] w ere examined t o AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus 119 119 119 119 119 120 119 121 118 119 159 159 159 159 157 158 157 160 158 159 199 199 199 199 197 198 197 200 198 199 VI ... o AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus 119 119 119 119 119 120 119 121 118 119 159 159 159 159 157 158 157 160 158 159 199 199 199 199 197 198 197 200 198 199 VI ... a glutamic acid residue affects t he kinetic parameters of the R197E mutant. The K 0.5 forBPGAistwicethatofthewild- type recombinant GAPDH and the catalytic constant is one half with NADPH as cofactor....

Ngày tải lên: 19/02/2014, 16:20

... mutants) and per-manganese metal basis (manganese superoxide dismutase mutants). Table 2. Metal contents of mutant superoxide dismutases. Metal con- tent was measured by atomic absorbance and is ... purchased from Pharmacia Biotech (Vienna, Austria). Visualization and analysis of molecular structures Molecular structures whose coordinates were obtained from the RCSB database were visualized ... Peplow, F. Yamakura and T. Matsumoto for the analyses of iron and manganese in protein samples. We also wish to thank H. Steinman for the gift of E. coli OX32 6A. We finally thank Mr M. Farrugia for...

Ngày tải lên: 21/02/2014, 01:21

... variants of TPP II ( i.e. rat, mouse, fruit fly, Arabidopsis thaliana, Caenorhabdit is elegans and Schizosaccharo myces pombe), and at least three human EST-clones covering this area (GenBank a ... standard assay and the i mmunoreactivity was detected by Western blot analysis and quantitated as described i n Materials and methods. Open and filled circles indicate the activity, and open and ... catalytic His264, and the proximity to the active site may explain the effect of oligomerization on enzyme activity. Even though the exact mechanism for complex formation and activation of the...

Ngày tải lên: 21/02/2014, 15:20

... the axis area are of polar character. The water channel in the apo form of the enzyme runs along the axis of the b-propeller through the whole domain and is only blocked by Cys236 in the area ... probably a consequence of a mistake incorporated by the TaKaRa Ex TaqÔ poly- merase and did not affect the enzymatic activity. MS The molecular mass of the recombinant b-fructofuranosi- dase was ... performs a nucleophilic attack on the anomeric carbon of fructose. The leaving group is carboxylate of Asp54. Dimerization The asymmetric units of the crystals of both the apo and complexed form...

Ngày tải lên: 06/03/2014, 00:21

... indicating that the thermal unfolding process of the precursor form follows a two-state transition. The ratio of the unfolding enthalpy (DH cal )tothe van’t Hoff enthalpy (DH v ) was 1.05 (DH cal and ... stabilization of proCPY against pressure. In contrast to the two-state transition of the precursor form, mature CPY showed a multistate transition: a first transition in the 0.1–150 MPa range, a ... or was obtained from Oriental Yeast Co. (Lot 21003805) (Osaka, Japan) and proCPY was prepared as the same manner as CPY, with minor modifications. Dgly CPY and Dgly proCPY, in which the asparagine...

Ngày tải lên: 07/03/2014, 21:20

... occurrence of a different type (type I) of fatty acid biosynthetic pathway in the human host from that of the malaria parasite, make this pathway an attractive target for developing antimalarial agents ... for the analysis of the conformational stability of other acyl carrier proteins. Abbreviations AAS, acyl-ACP synthase; ACP, acyl carrier protein; AcpS, holo-ACP synthase; apo-ACP, Plasmodium falciparum ... form and the holo form displayed a reversible two-state unfolding mechanism. The analysis of iso- thermal denaturation data provides values for the conformational stability of the two proteins. Although...

Ngày tải lên: 16/03/2014, 10:20

... significantly to the disappear- ance of nitrotyrosinated tubulin, the tyrosination/detyrosin- ation at the C-terminus of a- tubulin is the main operating mechanism. Furthermore, when cells maximally ... dysfunction. ACKNOWLEDGEMENTS We thank Drs Carlos E. Argaran ˜ a and Mario Guido for critical reading of the manuscript, Mrs S. N. Deza and Mrs M. G. Schachner for technical assistance and Dr Stephen Anderson ... Secretarı ´ adeCienciayTe ´ cnicadelaUniver- sidad Nacional de Co ´ rdoba y Agencia Co ´ rdoba Ciencia del Gobierno de la Provincia de Co ´ rdoba, Argentina. REFERENCES 1. Barra, H.S., Arce, C .A. ,...

Ngày tải lên: 17/03/2014, 10:20

... formation at the terminator site. When the adjacent uracil-rich sequence is being transcribed, the affinity of the dA–U base pairs is insufficient to stabil- ize the DNA–RNA duplex, and the transcript, together ... implicated in transcriptional activation and repression, regulation of alternative splicing, regula- tion of mRNA stability, translational activation or repression and RNA packaging. It was also shown recently ... sugar–phosphate backbone of the nucleotide in subsite 6 and the formation of the alternative subsite 7 are a consequence of the inaccessi- bility of Trp8 in this crystal form. In addition to their...

Ngày tải lên: 23/03/2014, 09:21

... D36N-1 5-CATCCTTCCCTATCTC AACATCCAGCTGGTTTACT-3 D341N-1 5-AAGGGGAGAACTC AACGGAACACCGGAGG-3 D389N 5-CACTCTCGAAGAGTTCATA AACGAAGTGAAGAAGAATCTC-3 5-GAGATTCTTCTTCACTTCGT TTATGAACTCTTCGAGAGTG-3 M. Karlstro ă m ... 5’-CCTGGAGAAATCGATCA TGAGCTTCGCTCAGTCGTG-3’ 5’-CACGACTGAGCGAAGCT CATGATCGATTTCTCCAGG-3’ F205M 5’-AAAAGGTCGACATCTGG ATGGCGACGAAAGACACGATC-3’ 5’-GATCGTGTCTTTCGTCGC CATCCAGATGTCGACCTTTT-3’ D36N ... different conformations, the large and the small domains of each homologue were compared separately. The rmsd values between the C a -carbons of the large domain of TmIDH and that of PcIDH and HcIDH...

Ngày tải lên: 23/03/2014, 10:21

... protozoa, CCT has been characterized at the gene sequence level in the ciliate Tetrahymena pyriformis [19,20], in the diplomonadide Giardia lamblia and the parabasilide Trichomonas vaginalis [21]. ... nondenaturing PAGE; the ratios of labeled to unlabeled tubulins are indicated on the top of each lane and the arrow indicates the position of the b-tubulin/CCT complex. (B) The amounts of the tubulin ... CCT were quantitated by the use of a phosphori- mager and expressed as a fraction of the maximum amount of bound labeled tubulin and plotted as a function of the ratio of labeled : un- labeled tubulin. Ó...

Ngày tải lên: 23/03/2014, 21:20

... a rather unimportant factor. The younger age during the first visit and the regular breast exam are positively correlated with the choice of a male gynecologist. Other parameters, such as ... JOURNAL đ VOLUME 4, ISSUE 1 (2010) using as a dependent variable the potential preference of a male gynecologist, the age of the first visit and the breast exam presented, in comparison to the ... social relationships. According to the literature, the patients believe that a female gynecologist is more compassionate and largely possesses the so called humanitarian values, whereas there...

Ngày tải lên: 28/03/2014, 14:20

... licensee Is the obligation a financial / monetary liability? Advance payment may be made to licensor Is the asset a financial/monetary asset? Is the value of the asset fully recoverable? Development and ... releasing the advance to the income statement as revenue only as the publisher generates actual sales, rather than recognising in full as under the rst example. The assessment of an advance ... operations rather than manage the design, manufacture, shipping and sale of team branded merchandise. Teams therefore outsource this function to a third party, typically a sportswear manufacturer....

Ngày tải lên: 29/03/2014, 20:20

... the case of CyPB in complexes with Suc-Ala-trans-Pro- Ala-pNA and Ac-Ala-Ala-trans-Pro-Ala-AMC, the large distortion in the orientation of Ala1 and Ala2 of Ala-Pro with //w torsional angles of ... using a 32-mer primer of 5Â-AAAAAGAAT TCATCGATATGTTCAAATCGACC-3Â with EcoRI added at the 5Â end of ClaI, a 23-mer primer of 3Â-CGAGA CGGCATTCCTAGGTTTTT-5Â, and pATtrpEPPIa as a template. The P ... [5,17,18,26–29] and shorter peptides such as Ala-Pro, Ala-Pro-Phe, Ala-Ala- Pro-Ala and Ala-Ala-Pro-Ala-Ala i nhibit cis to trans isomerase activity of calf thymus CyPA [27]. It was suggested that in the...

Ngày tải lên: 30/03/2014, 15:20

Ngày tải lên: 30/03/2014, 20:20

Ngày tải lên: 30/03/2014, 23:20

Ngày tải lên: 31/03/2014, 07:20