the roles of yeast in bread making

... and Schroer, 2000) There are two types of kinesin, kinesin-1 and kinesin-2 Kinesin-1 is found to be involved in the transport of various organelles including Golgi complex (Lippincottschwartz et ... ones containing Tub1p as the only α-tubulin were more dynamic as compared to MTs in the wild type Their data indicate that the minor α-tubulin isoform Tub3p can stabilize the yeast MTs in vitro ... VirE2 in vitro and the binding site in VirE2 overlapped with the VirE2 self-binding domain, indicating that VirE1 may protect VirE2 from self-aggregation (Deng et al., 1999) The binding site in...

Ngày tải lên: 09/09/2015, 10:08

... INVESTIGATIONS ON THE ROLES OF UBIQUITIN IN THE REGULATION OF HEAT SHOCK GENE HSP70B’ EE THIAM HEE, GARY NATIONAL UNIVERSITY OF SINGAPORE 2011 INVESTIGATIONS ON THE ROLES OF UBIQUITIN IN THE ... REGULATION OF HEAT SHOCK GENE HSP70B’ EE THIAM HEE, GARY (B SCI (HONS), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MICROBIOLOGY YONG LOO LIN SCHOOL OF MEDICINE NATIONAL ... OF PHILOSOPHY DEPARTMENT OF MICROBIOLOGY YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE 2011 ...

Ngày tải lên: 10/09/2015, 08:27

... Each of these modifications has their role in the regulation of transcription In this project we investigated the role of ubiquitin in the regulation of the heat shock gene HSP70B’ Through RNA interference ... Partner of Skp1 104 3.6 The Role of Med21/hSrb7 in the Regulation of HSP70B’ 109 3.7 The Effect of the RNA Interference of Med21/hSrb7 and its Effects on Proteosomal Inhibition of HSP70B’ Induction ... Determining the Existence of Ubiquitinated Species of Hsf1 137 3.13 The Stability of Hsf1 141 3.14 The Presence of Hsf1 at the HSP70B’ Promoter 149 vi Chapter 4: Discussion 158 4.1 Key Findings of...

Ngày tải lên: 10/09/2015, 08:27

... isoleucine 44 in ubiquitin is the binding site of these ubiquitin binding domains (UBDs) despite the different structural folds in each UBD resulting in minor variations with their exact interaction ... proteins can greatly hinder their ability to bind to ubiquitinated cargo This is mainly due to the ubiquitin molecule interacting with their ubiquitin binding domains and “occupying” these domains ... Another means of conferring specificity is through the use of the structural differences inherent in the types of ubiquitin chains K-48 chains tend to be more “kinked” in structure while the...

Ngày tải lên: 10/09/2015, 08:28

... increase the protein level of ATF3 in gonadotropes These findings would lay the foundation for investigating whether Pin1 plays a role in transcriptional regulation of αGSU gene v LIST OF TABLES ... in activating SF-1, in which the C-teminus of Pitx1 interacts with the putative ligand binding domain (LBD) of SF-1 to enhance transcriptional activity of SF-1 through an apparent unmasking of ... N-terminal DNA binding domain (DBD) including two zinc fingers, a hinge region with an activation function domain (AF)-1 in its distal part, and a ligand binding domain with a conserved C-terminal...

Ngày tải lên: 11/09/2015, 09:57

... The contexts of research in overseas: In an overview of the research in the world, the thesis refers to the group of problems: 1) Studies which involve the roles of the Government in building ... Studying the contents of related issues to the roles of the Government in social welfare for farmers; 3) Studying the conditions to ensure the implementation of the Government's roles in the development ... appendices of the processing of information, data and survey results as well as the contents of the four following chapters To create the conditions to implement the roles of the Government in social...

Ngày tải lên: 25/12/2015, 17:21

... indicate the net utilization of minerals Absorption of mineral ions is dependent upon numerous factors, including the levels of the elements ingested, the age of the animal, Ph of the intestinal ... involvement of phosphorus in the ATP mol ecul e are two exampl es of the di verse roles of minerals in energy production In their roles of growth and maintenance of tissues, minerals contribute to the ... of soft tissue; fluorine, zinc, and silicon play roles in the formation of proteins and fats that compose the body (8) Minerals also preserve cellular integrity through their roles in maintaining...

Ngày tải lên: 15/02/2014, 16:20

... substrates: in the first, a (small) sub population of enzyme binds directly the steroid in the reactive conformation, with the remainder of the enzyme binding the unreactive conformation Rescue of the ... of the enzyme-steroid complex We conjectured that the reduced form of the enzyme might direct binding of the steroid in the flipped conformation, thus facilitating catalysis Our structure of the ... conformation involves release of steroid and re-binding in the reactive conformation In the second model, the steroid flips its conformation from the unreactive to reactive binding mode whilst resident in...

Ngày tải lên: 20/02/2014, 02:21

... to the regions of the protein involved in interaction with Cdk2 and cyclin A: 1, domain interacting with cyclin A; 2, a linker helix involved in binding both cyclin A and Cdk2; 3, domain interacting ... binds and rearranges the amino-terminal lobe and also inserts into the catalytic cleft, mimicking ATP in the context of the cyclin A–Cdk2 complex [120] In contrast, the unbound p27Kip1 Cdk-inhibition ... with binding diversity and helps to explain the role for structural disorder in mediating binding specificity in biological systems [66] The p27Kip1 protein is another member of the p21 family of...

Ngày tải lên: 07/03/2014, 21:20

... knowledge of the biosynthesis of DIMs and PGLs, the in vivo functions of these FadD proteins in this pathway remain to be established and ⁄ or completed For instance, the involvement of FadD26 in the ... Schematic representation of the roles of the FadD proteins encoded by the DIM + PGL locus in the biosynthesis of DIMs and related compounds in Mycobacterium tuberculosis The role of the activation enzymes ... onto PpsA during DIM biosynthesis prompted us to examine whether another FadD protein is specifically involved in the activation of the PpsA substrates during the formation of PGLs Among the fadD...

Ngày tải lên: 15/03/2014, 11:20

... terms on some of the individual loans issued by a sample of banks, including the largest banks in the nation Beginning in 1997, the STBL includes the banks’ risk ratings on their individual loans, ... by the one or more of the inside owners, giving the financial institution recourse against their personal wealth in the event the loan is not repaid In many cases, the personal assets of the insiders ... information of the intermediary with the business, the business owner, or other involved then uses this information contract terms at origination garnered about the initial quality of the small business...

Ngày tải lên: 15/03/2014, 21:20

... producing 50% inhibition of the inhibitable component, U0 is the uptake in the absence of the inhibitor, Unoninh is the uptake which cannot be inhibited and ni is the Hill coefficient for the inhibitor ... [3H]cGMP in the absence of ATP but in the presence of the nonhydrolysable ATP analogue, ATP-c-S, was less than 5% of the uptake in the presence of ATP The amount of [3H]cGMP taken up by these vesicles ... numbers of fitting parameters were compared using an F-test on the ratio of the variance associated with the reduction in degrees of freedom to the variance of the fit with the smaller number of degrees...

Ngày tải lên: 23/03/2014, 21:20

... which in terms of the logic of inquiry, precedes the evaluative 270 SINCLAIR THE GENERATIVE ROLE OF THE AESTHETIC The generative role of the aesthetic may be the most difficult of the three roles ... values—some of the elements of the affective domain (Goldin, 2000)—that are intertwined with aesthetic responses The three roles of the aesthetic in mathematical inquiry that I have identified have their ... within the same domain as the problem The evaluative aesthetic is not only involved in judging the great theorems of the past, or existing mathematical entities, but is actively involved in mathematicians’...

Ngày tải lên: 30/03/2014, 16:20

... tube, along the nozzle, and in the surrounding air The length of the plasma plume can reach as long as cm with helium as working gas To investigate the role of the charged particles in the inactivation ... range, are playing the main role in the inactivation process Finally, the measurements of the UV intensity show that the UV emission is too weak to play a crucial direct role in the inactivation ... hollow barrel of a syringe The diameter of the hollow barrel is about mm and the diameter of the syringe nozzle is about 1.2 mm The distance between the tip of the HV electrode and the nozzle is...

Ngày tải lên: 18/05/2014, 20:36

... way of doing things Your creative thinking will also lead to find out whether the efficiency of an activity is improved by making the posture of the one performing comfortable, by better lighting, ... and company goals therefore increasing the bottom line In other areas of training Jan has excelled in defining critical business needs and delivering to achieve outcomes using techniques and ... way? The above listed mind probing questions will lead to thoughts of finding better ways of doing things by simplifying and improving the ways of doing things, discarding activities that serve...

Ngày tải lên: 28/06/2014, 17:20

... has increased stability and binding in hypoxic conditions and is degraded rapidly in normoxia The accumulation of the α-subunits allows for αβ heterodimer formation and translocation into the ... nucleus during hypoxia This process leads to selective upregulation of genes whose products are involved in hypoxia and inflammatory lung injury These include erythropoietin, vascular endothelial ... and lisofylline, a phosphodiesterase inhibitor, are described as being able to reduce proinflammatory cytokines and ameliorate oxidant lung injury in animal models As exciting as this emerging field...

Ngày tải lên: 12/08/2014, 19:21

... providers into a grey area of supporting life where there is increasingly little hope of recovery The result is a shift towards prolonging death This situation is found in critical care units around the ... realistic?” They ask questions about the appropriateness of treatment plans and sometimes feel they are unable to act in the best interest of the patient and family [2] The issue of futility ... different from the treating team The cycle of blame and unyielding applications of salvage treatments take the place of open communication and clear plans Another approach In the moment, decisions...

Ngày tải lên: 12/08/2014, 19:22

... overlapping sites Given the large number of potential binding sites in a localized region of 3' UTR, the increased repression of multiple pairs of binding sites can be explained by having more binding ... environment in which other binding sites reside, so that the repression of the transcript by the original miRNA may be mediated by other factors; second, the probability of finding a conserved binding ... interactions multiple pairs of overlapping binding sites These links to diseases underline the importance of studying the mechanisms behind miRNA targeting, which we discuss in the following refereed research...

Ngày tải lên: 14/08/2014, 08:20

... important roles for determining the timing of tissue differentiation during larva period of Drosophila development and maintaining the tissue identity during the adulthood It should be noted that the ... genes These findings strongly suggest that miRNAs play a most important role in driving tissue terminal differentiation and maintaining tissue identity rather than in determining or regulating ... understanding the global role of miRNAs in maintaining lineage of differentiated tissues and cells has great impact on the studies of miRNAs in cancer genetics 16 Methods Datasets used in this study The...

Ngày tải lên: 14/08/2014, 16:20

... suggesting in another language This leads the author to the research into The use of politeness strategies in making suggestion in English and Vietnamese to find out the similarities and differences in ... in making suggestion in Vietnamese and English II Aims of the study The aims of the study are: - To investigate ways of suggesting in Vietnamese and English - To compare and contrast the use of ... suggesting III Scope of the study In all aspects of politeness, the study only deals with verbal aspects, in the light of the politeness theory by Brown and Levinson The paper is aimed to investigate...

Ngày tải lên: 28/02/2015, 11:54