... infectious disease of our day High numbers of infections occur all along the equator from Africa over the Middle East to Southeast Asia with China and Russia also sharing a big part ofthe global TB burden ... 5’ ATGGTACCGTGTCC GAATTGACTGTGCG 3’ 5’ TA GGATCC CCG AAG AAC ACA AAC ACC GC 3’ Amplification of menB (Rv0548c) Amplified region: -101 ; +462 = 563 bp 5’ ATGGTACCTCACGA CCACATGTGACCAAG 3’ 5’ TAGCATGCGACCAGACAAAT ... TAGCATGCGACCAGACAAAT GACCACCTT 3’ Amplification of menC (Rv0553) Amplified region: -112 ; +504 = 616 bp 5’ AT GGTACCAGTACG CGGATATGGTGGTG 3’ 5’ TAGCATGCACAGGGTTGTTC AAGGTATTC 3’ Amplification of...
... study, there was a significant association of age, grade, lymph node spread and vascular invasion with distant metastases free survival (MFS) in all invasive carcinomas and the subgroup of IDC in an ... years younger than patients without distant metastases (54 years) [p = 0.02] in the IDC group For all invasive carcinomas, the average age of patients with distant metastases was 46 years compared ... of distant metastases in all invasive carcinomas, the subgroup of invasive ductal carcinomas and in the node negative group when cyclin E was stratified as negative and positive (low/high) Many...
... research, contributed to protocol planning, was a primary data gatherer and drafted the manuscript SC participated in data collection and statistical analysis VN performed statistical analysis ... variables associated with death Variables that were associated with mortality in univariate analysis were considered for inclusion in the model, whereas parameters already incorporated into the ... ventilation, length of stay, and the number of failing organs Statistical analysis Continuous variables' data are expressed as mean value ± SD Bi-variate hypotheses involving continuous variables...
... evaluations of who was to pay for the clear-up after the environmental disaster Problems arose again in 1997, this time after the national TV channel (DR1) ofthe Danish Broadcasting Corporation broadcast ... (UHÄ) The location ofthe general study programmes and the admission capacity of each of these were decided by the Government and Parliament on the basis of material from UHÄ Also, most ofthe ... The Copenhagen area has seen an actual net influx of inhabitants in the past 10 years after many years of marked “emigration” The change in settlement pattern in the past thirty years is closely...
... Qualitative data as an attractive nuisance: the problem of analysis Administrative Science Quarterly 24: 590–601 Nonaka I 1994 A dynamic theory of organizational knowledge creation Organization ... instead In the above case, the corporate involvement in terms of stimulating local management was primarily through the award routine The regular financial reporting between general managers and their ... in the way that we have Theroleof motivation is probably as important in a chain of hotels or supermarkets, a software vendor or a consulting company, regardless ofthe nature of knowledge The...
... National Association of State Public Health Veterinarians Keith A Clark, D.V.M., Ph.D Texas Department of Health Austin, TX National Vaccine Program Anthony Robbins, M.D Office ofthe Assistant ... College of Chest Physicians University of Alabama at Birmingham Birmingham, AL Wafaa M El-Sadr, M.D., M.P H Infectious Disease Society of America New York, NY Alice Y McIntosh American Lung Association ... regarding the risks and benefits associated with both BCG vaccination and TB preventive therapy They should be informed about a) the variable data regarding the efficacy of BCG vaccination, b) the...
... W168F Y74W CACCATGGCTAGAAAATATTTTGTCGCAGCAAACTTCAAATGTAA GAACCTTTATTCGCTATTGGTACCGGTAAA GAACCTTTATTCGCTATTGGTACCGGTAAA TCACCGGTCCATGATCCATT NcoI KpnI KpnI HaeIII 4178 FEBS Journal 276 (2009) ... maintaining the geometry ofthe active site The availability of crystal structures of TIMs from 21 sources and the large database of TIM sequences from various sources facilitate an analysis of ... (Agilent Technologies, Santa Clara, CA, USA) Nebulization was assisted by N2 gas (99.8%) at a ow rate of 10 Lặmin)1 The spray chamber was held at 300 C The spectrometer was tuned using ve calibration...
... such that either the HA or the HIS epitope tag was added to the end ofthe ESSS ORF The forward primer was: 5¢-ACga atccGATCTCCGACCCA-3¢; the reverse primer was: 5¢-ATgctagcCTCATCTTCTGGTAACTGG-3¢ ... was used per assay Oxygen consumption was measured polarographically with a Clark oxygen electrode in metabolic chamber with a water jacket maintained at 37 °C (Hansatech, Norfolk, UK) Substrates, ... with HA- or HIStagged hamster ESSS Complex I activity was measured asthe malate/glutamate-induced, rotenone-sensitive activity, and the activity ofthe downstream portions ofthe electron transport...
... confirmed by DNA sequencing DNA sequencing Plasmid DNA was isolated with the GFXTM Micro Plasmid Prep Kit (Amersham Pharmacia Biotech, Piscataway, NJ, USA), and the resulting dsDNA was mixed with ... the DSC curve The enthalpy change (DHcal) of each protein was calculated by integration ofthe curve covering area (Tm was taken asthe curve peak point) using origin software Acknowledgements ... were purchased from Boehringer (Mannheim, Germany) Ethanol (> 99%) was obtained from Panreac (Barcelona, Spain) Urea was a product of Acros (Pittsburgh, PA, USA) The QuickchangeTM kit containing...
... 5¢-d(TCAGAAATTAAAGCGG ATGCATTATTTGCATG)-3¢ The upstream primer containing the NdeI restriction site (underlined) was: 5¢-d(GCTAGCATATGAAGCTTAAA AAAATTG)-3¢ and the downstream primer containing the ... Ala, upper primer 5¢-d(ATAGATTGGGGTGCCCATGCAAATAA TGCA)-3¢, lower primer 5¢-d(ATTATTTGCATGGGCA CCCCAATCTATTTG)-3¢; Tyr269 to Ala, upper primer Fig Partial alignment of alkaline phosphatases at ... higher than the native cold adapted enzyme (Table 1) The mutant G26 1A/ Y26 9A exhibits an Ea almost the same as in the case ofthe native enzyme (Table 1) Thermal inactivation of mutant and wild-type...
... have to take into account the evolution of real wages as well asof wage arrears However, a remarkable point is that a substantial proportion of firms (31.5% ofthe sub-sample) has increased their ... qualitative survey may actually offer a stronger appraisal of their actual behaviors Section two ofthe paper presents a brief description ofthe data base and the way it has been used here The third section ... would be a set of given, frozen parameters, rather than adjustment variables on which the managers strategies would actually be able bear Hence the importance of inertial variables, such as past indebtedness...
... Diagnoses and Classifications Classification of Primary Joint Diseases Classification of Secondary Joint Diseases Hyperplasia, Hypoplasia, and Aplasia ofthe Condylar Process Hyperplasia ofthe ... radiograph Mucosal changes 11 Anatomy ofthe Masticatory System A rational clinical examination ofthe masticatory system requires a sound basic knowledge ofthe anatomy As will become clear later ... Mucogingival pain Ostealgia m6 periosteal pain Cutaneous pain jyjpaln Deep pain — Migraine with aura Migraine without aura Cluster headache Paroxysmal unilateral headache Neurovascular variants Arteritis...
... pKa calculations pKa values for Asp140, Asp142, Glu144 and Asp215 were calculated in several ChiB mutants as described in Materials and methods (Table 3) Calculations were primarily based on the ... mutant retains considerable activity, whereas the D14 2A mutant does not It has been shown by X-ray crystallography that replacement ofthe Asp142 analogue by alanine in other family 18 chitinases ... The pKa of Asp140 is much lower than that of Asp142 and Glu144 in all situations where all three residues are present and the one proton shared by Asp140 and Asp142 appears to remain on Asp142...
... standard analytical procedures and simple theorems that can be applied again and again to apparently dissimilar economic problems The student of microeconomics can exploit the fact that many basic ... problems are typically associated with harder mathematics The material in Appendix A is intended to be a statement of fair dealing concerning the technical requirements ofthe main text: there are ... ofthe game could refer literally to a game (see chapter 10) but, even in models where game theory makes no formal appearance, the assumptions about the forms of action and interaction that are...
... three days later and asked if they had read the instructions (attention) If they had, they were asked a series of questions about information in the handout (recall) and also about what they had ... ofthe readers, all of whom had gout, asa possible reason for the lack of differences between the groups as well as page layout issues The fifth study, by Morrell et al [33] compared recall of ... novel about asbestos hazards or an NCI asbestos pamphlet were mailed, with an evaluation questionnaire, to a random sample of 500 members ofa building trades union There was a 21% response rate...
... procedure according to a protocol from the manufacturer GTATTCAAAAGTGGTCCCGGACAAAATGAGGACTTG TCCGGGACCACTTTTGAATACTTTCAAGTGCATATATTTATT CAAAAGTCTTGGCGGACAAAATGAGGACTTGGTAC CATTTTGTCCGCCAAGACTTTTGAATACTTTCAAGTGC ... CATTTTGTCCGCCAAGACTTTTGAATACTTTCAAGTGC CTTCCCGGAGGAAATGAGGACTTGGTACTTACTG CCTCATTTCCTCCGGGAAGACTTTTGAATAC CGGACAAGGTGAGGACTTGGTACTTACTGGATAC CAAGTCCTCACCTTGTCCGGGAAGACTTTTG Proteases Papain (EC 3.4.22.2) was purified, ... L73G-cystatin A cathepsin B complex was obtained from the analyses ofthe association kinetics (see above) asthe intercept on the ordinate ofthe plot of kobs,app vs inhibitor concentration and was...
... In all cases, the optimal amount of arachidonic acid was determined and used to analyze the effect of S10 0A8 /A9 on oxidase activation After an incubation of 10 at 20 °C, the oxidase activity was ... an optimal amount of arachidonic acid determined for each assay of oxidase activation [12] The rate of O2– production by the activated NADPH oxidase was calculated from the rate ofthe superoxide ... NADPH oxidase activity was also assayed by polarographic measurement ofthe rate of O2 uptake at 20 °C with a Clark electrode at a voltage of 0.8 V All experiments were carried out at least twice...
... define the pulling coordinate for theA and G b-strands asthe distance between the first amino acid of strand A (Y9) and the last amino acid of strand G (K87) This distance, x(Y9)ÀxACHTUNGRE(K87), ... unfolding the I27 protein in D2O The pulling coordinate for the separating b-strands is defined asthe distance between the first amino acid of strand A (Y9) and the last amino acid of strand G ... G (K87) The elongation ofthe x(Y9)Àx(87) distance up to the transition state is defined asthe distance DxA’ÀG The crossing ofthe transition state is marked by an abrupt rapid increase in x(Y9)Àx(87)...
... (AA) and measures all the adjustments made when using the accrual basis of accounting This is termed ‘aggregate accruals’ in the paper: E - NCF = AA = ACNA - ACL + ALTNA,, - ALTL, - ALTL,, + ALTNA, ... performance declines asthe absolute value of aggregate accruals [abs(AA)] increases In this test, all firm-period observations are ranked on the basis of ah(M), quintiles are then formed, and separate ... small and cash flows and earnings are most similar, cash flows are a relatively useful measure of firm performance However, when the magnitude of accruals increases, indicating that the firm has...