... with the indicated antibodies before the addition of the probe. The a-LIN9 antiserum has been described previously [12]. The LIN54 antiserum was raised in rabbits against the peptide AAKTKLSSQISDLLTR. ... in transfection efficiency. Acknowledgements We thank all members of the laboratory for their sug- gestions and critical reading of the manuscript. This work was supported by grants from the DFG ... previously that the depletion of LIN9 in human cells inhibits proliferation and results in delayed entry into mitosis [9]. To address whether this is an isolated function of LIN9 or whether it is medi- ated...
Ngày tải lên: 23/03/2014, 04:20
gabon an example for all of africa
... 1995 )The currency of Gabon, the CFA Franc, is not worth much compared to the dollar. Theexchange rate was for every US dollar there is 529.43 CFA Francs in 1995. The per capitaincome is twice as much ... most advanced and extensive air transportnetworks. They have a total of 69 airports. Thirty eight of these airports have paved runways. Even though the runways may be paved, many of the roads ... Yet another reason for Gabon's success is its economy. Gabon is an oil-rich country. Oil accounts for 80% of their exports. Besides petroleum, substantial timber resources andexpansion of...
Ngày tải lên: 21/03/2014, 22:02
Báo cáo khoa học: A novel factor XI missense mutation (Val371Ile) in the activation loop is responsible for a case of mild type II factor XI deficiency doc
... this position most likely causes some degree of structural change; this is especially true in the case of the introduction of an isoleucine, a b-branched amino acid that is not flex- ible. In the ... calculated on the basis of known concentration of wild-type and mutant FXIa and using the program grafit (Erithacus Software Ltd., Staines, UK). Structural analysis The structural analysis was conducted ... De Cristofaro 4 , Giancarlo Castaman 3 and Stefano Duga 1 1 Department of Biology and Genetics for Medical Sciences, University of Milan, Italy 2 Department of Biochemistry, George S. Wise Faculty...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa hoc:" Genetic analysis and management in small populations: the Asturcon pony as an example" docx
Ngày tải lên: 09/08/2014, 18:21
Báo cáo y học: "Is obesity a risk factor for low back pain? An example of using the evidence to answer a clinical question" ppsx
Ngày tải lên: 13/08/2014, 13:22
Tài liệu An Example of Using the Get* Methods phần 1 pdf
... this while loop, each of which is used to store the result from the Get* method. For example, the productID variable is used to store the ProductID column value, and since ProductID is of the ... productsSqlDataReader.GetDataTypeName(productIDColPos)); This example displays: ProductID database type = int As you can see, the ProductID column is of the SQL Server int type. Listing 9.2 uses the code examples shown in this section. Listing ... that is used to store the ProductName column value. This column is of the nvarchar SQL Server type, and to get the Product-Name column value, the GetString() method is used. Of course, this...
Ngày tải lên: 24/12/2013, 01:17
Tài liệu An Example of Using the Get* Methods phần 2 docx
... SQL Server, always use the GetSql* methods and Sql* types rather than the Get* methods and the standard C# types. I showed you the Get* methods earlier only because they work with non-SQL Server ... SqlBoolean discontinued = productsSqlDataReader.GetSqlBoolean(discontinuedColPos); Console.WriteLine("discontinued = " + discontinued); } Listing 9.3 uses this while loop. Listing ... take a look at an example that reads the ProductID, ProductName, UnitPrice, UnitsInStock, and Discontinued columns from the Products table using the GetSql* methods. To figure out which GetSql*...
Ngày tải lên: 24/12/2013, 01:17
Tài liệu The 2012 Nexus Event – An Unknown Form Of Energy Is Coming Our Way pdf
... what is illustrating you can clearly see that the arrow of the Sagittarius is indicating an explosion day and the arrow of the Sagitta is the result of that explosion. In other words, the cosmic ... time or that is already researched thermally and no other scanning on checking is necessary. But the genius of this man is extraordinary and he often founds more and more parts of the puzzle ... increases, which disturbs the stability of each planet including Earth. The passage to the densest portion of the gravitational plane is the direct cause of the devastating cycles and pole shifts...
Ngày tải lên: 19/02/2014, 03:20
Báo cáo " The effects of pictures on the reading comprehension of low-proficiency Taiwanese English foreign language college students: An action research study " ppt
... assist them in catching the gist of the passage and improving their comprehension. However, a majority of students disagreed with the effectiveness of using visuals to guess the meanings of ... understanding of text that exceeds their proficiency level? The hypothesis of this study is that regardless of whether text meets or exceeds a student’s proficiency level, the addition of pictures ... facilitated their comprehension of not only the simpler but also the more difficult text. These results are in accordance with the hypothesis, they support the results of previous studies, and they...
Ngày tải lên: 05/03/2014, 12:20
Báo cáo Y học: Ferritin from the spleen of the Antarctic teleost Trematomus bernacchii is an M-type homopolymer doc
... solvent shown by the red shift of the maximum emission fluorescence wavelength relative to the protein at pH 3.0. Analysis of the iron-incorporation process is i n accord- ance with the presence in ... desalted using the ZipTipC18 (Millipore) and then mixed with the matrix solution (a-cyano-4-hydroxy- cinnamic acid) for MALDI-TOF MS analysis. Structure comparison A search of the SwissProt-TrEMBLE ... by MS analysis. The automated Edman degradation o f the native protein was unsuccessful for the possible p resence o f a blocked N-terminus. MALDI-TOF MS an alysis of a tryptic digest of the protein...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo Y học: Domain V of m-calpain shows the potential to form an oblique-orientated a-helix, which may modulate the enzyme’s activity via interactions with anionic lipid potx
... glycine residues in the polar face of this a-helix could play an important role in facilitating this mechanism of membrane penetration. This a-helix has a preference for anionic lipid, which leads to higher ... plot analysis of protein segments. Hydropathy plot analysis of the m-calpain domain V segment, GTAMRILGGVI, was performed using a seven-residue window and the software of Hennig [39]. It can be ... conformational analysis of VP1 in the presence of lipid. (A)–(C) Spectra representing FTIR conformational analyses of VP1 in the presence of lipid with annotated numbers indicating band peak absorbances....
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khoa học: A strategy for the generation of specific human antibodies by directed evolution and phage display An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom docx
... venom. This is the first recombinant human antibody fragment that neutralizes C. noxius venom. To the best of our knowledge, this is the first report of the generation of a human recom- binant antibody ... of these changes occurred at frame- work 3 of the heavy chain (Asp74Asn) and 3 of the light chain. Two of the changes (Thr152Ile and Ser197Gly) occurred at frameworks 1 and 3, respectively, and ... stable and ⁄ or low affinity variants. The scFv 6009F blocked an epitope in Cn2 which seems to be very relevant for the interaction of the toxin with its target. These are the first recombinant human...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo Y học: The C-terminal domain of perfringolysin O is an essential cholesterol-binding unit targeting to cholesterol-rich microdomains pptx
... folds into the secondary structure of the native toxin. On the other hand, the spectra of the T2 and T2¢ fragments exhibit more disordered structures than that of D4. These data imply that their extra ... during membrane binding, we analyzed the susceptibilities of the T2¢ and D4 fragments to protease in the presence and absence of membranes (Fig. 3). In the absence of liposomal Fig. 1. Isolation of toxin ... However, the maximal emission wavelength of the intrinsic tryptophan fluorescence is red- shifted in the D4 fragment (Fig. 4), which indicates that the environment of the tryptophan residues in the...
Ngày tải lên: 23/03/2014, 21:20
An Account of Some of the Principal Slave Insurrections, and Others, Which Have Occurred, or Been Attempted, in the United States and Elsewhere, During the Last Two Centuries. pptx
... on them, in the most earnest manner, the duty of immediately abolishing slavery as their only hope of safety, the only means by which they can escape the just judgments of God. The safety of ... a picture in the history of this unhappy island, occurred in the days of slavery, before the proclamation of Santhonax and Polverel, and before the great conventional decree of the mother country ... increased persecution of the free people of color, a law to prevent their increase, and a desire to banish all of them from the State. The conspiracy of Gen. Gabriel and his coadjutors was, therefore, the occasion,...
Ngày tải lên: 31/03/2014, 11:20
a tear is an intellectual thing the meanings of emotion feb 2000
... expres- sion. My thesis on the contrary is that the bodily changes follow directly the perception of the exciting fact, and that our feeling of the same changes as they occur is the emotion. Common ... Among them are the relation of the normal and the pathological, the relation of individual development and cultural history, the nature of explanation and evidence, the two faces of many emotions ... scream- ing/howling and irritating particles), and if only one of the two types of situation (direct irritation of the eye) arises and so stimulates the lacrimal glands in animals other than human beings, then...
Ngày tải lên: 10/06/2014, 21:33
– ACT SCIENCE REASONING TEST PRACTICE – 48. Which of the objects represented on Graph I is ppt
... heating of the air above the land and ocean. Land breeze occurs at night when the air above the land is cooler and the air above the ocean is warmer. The breeze blows from the land to the sea. – ... rest and exercise, therefore it is reasonable that the title of the graph would be Heartbeats per Minute During Rest and Exercise. There is no mention of the gender of the participant on the graph, ... transformation of that substance. Plate tectonics The theory in which Earth’s crust is made up of many plates that float on the mantle. This theory explains the movement of the continents, the...
Ngày tải lên: 18/06/2014, 17:20