the purchase price of a noload fund is determined by

Ngày tải lên: 09/03/2014, 02:20

... mutants) and per-manganese metal basis (manganese superoxide dismutase mutants). Table 2. Metal contents of mutant superoxide dismutases. Metal con- tent was measured by atomic absorbance and is ... MnSOD increases by half as much again (Table 1). We measured the metal content of the mutant proteins by atomic absorbance spectroscopy. The results of this analysis are presented in Table 2, with ... purchased from Pharmacia Biotech (Vienna, Austria). Visualization and analysis of molecular structures Molecular structures whose coordinates were obtained from the RCSB database were visualized...

Ngày tải lên: 21/02/2014, 01:21

... dehy- drogenase distal factor (Adf-1) element using the specific primer 5¢-CCGTCGACATTAATTTGAGAAATTATAT TGCGTCGCccg ccggcCgcCacgGAGGGTGAC-3¢ (again the SalI r ecognition site is i n bold, the location of ... is boxed, and the GAGA and Adf-1 elements in the DNA are underlined. The translation start codon and the main transcription start point are larger and in bold. (B) Mutations either in the G AGA ... sites of the D. melanogaster a -F1-ATPase gene We have previously characterized a cDNA encoding the D. melanogaster a- F1-ATPase subunit [27]. To isolate the corresponding D. melanogaster a- F1-ATPase...

Ngày tải lên: 07/03/2014, 16:20

Ngày tải lên: 16/03/2014, 23:20

Ngày tải lên: 17/03/2014, 10:20

Ngày tải lên: 29/03/2014, 23:20

Ngày tải lên: 20/06/2014, 07:20

... The Marketing Strategy of a multinational join stock company multinational join stock company has a certain advantage: a multinational join stock company has always kept its prices as competitive ... quality or the quality of the maintenance work that its staff have done, but has complained about the late reply and maintenance service of the company. The reason for this is the company’s not ... sold 2 famous brands that are Toshiba (Japanese), Mitsubishi (Japanese). Then recently it has expanded and started to sell 2 other brands: Trane (American) and Sanyo (Japanese). This is helped...

Ngày tải lên: 27/10/2012, 16:51

... are also made in the child DataTable. This is the default. None Indicates that no action takes place. SetDefault Indicates that the DataColumn values in the child DataTable are to be set to the ... to the database. By default, DeleteRule is set to Cascade; therefore, when you delete a DataRow in the parent DataTable, any corresponding DataRow objects in the child DataTable are also deleted. ... Cascade; therefore, when you change the DataColumn in the parent DataTable on which the ForeignKeyConstraint was created, then the same change is also made in any corresponding DataRow objects in the...

Ngày tải lên: 24/12/2013, 01:17

... o AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus 119 119 119 119 119 120 119 121 118 119 159 159 159 159 157 158 157 160 158 159 199 199 199 199 197 198 197 200 198 199 VI ... GAPDH obtained by m olecular modelling (Fig. 1A) , and that of chloroplast spinach GAPDH [35] w ere examined t o AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus AthalianaB PativumB SoleraceaB NtabacumB A. thalianaA PsativumA SoleraceaA Chlamy Synechocystis Synechococcus 119 119 119 119 119 120 119 121 118 119 159 159 159 159 157 158 157 160 158 159 199 199 199 199 197 198 197 200 198 199 VI ... residue affects t he kinetic parameters of the R197E mutant. The K 0.5 forBPGAistwicethatofthewild- type recombinant GAPDH and the catalytic constant is one half with NADPH as cofactor. Residue Arg197...

Ngày tải lên: 19/02/2014, 16:20

... share the same latent semantics profile, as long as they are in the same topic/domain. To solve the sparsity issue we use missing words as negative evidence of latent semantics, as in (Guo and Diab, ... as our disambiguation algorithm. It is a graph- based algorithm, where nodes are senses, and edge weight equals to the sense pair similarity. The final answer is chosen as the sense with maximum ... link the senses across dictionaries, hence Wik is only used as augmented data for WMF to better learn the semantics of words. All data is tokenized, POS tagged (Toutanova et al., 2003) and lemmatized,...

Ngày tải lên: 19/02/2014, 19:20

... Clone pRKX3, a derivative of tRNA Gly 1 -1 has a single TATA ele- ment at )130 bp and is transcribed to the same levels as the parent. pmutRKX3 has the single TATATAA element of pRKX3 mutated to GATATCA. ... of fragments containing the TATATAA sequence, but not by the mutated GATATCA sequence (Fig. 7A; lanes 3, 4, 6, 7 and 9). Inhibition by TATATAA-containing fragments was reversed by supplementation ... a multigene family is regulated by transcription factor TFIIIB Akhila Parthasarthy and Karumathil P. Gopinathan Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore,...

Ngày tải lên: 20/02/2014, 02:21

... chronic pain are available. 4.2.2 Informal and Community Care Costs Informal care costs are the value of the care provided by informal friends or family carers. This report analyses the available ... Demographic Characteristics by Pain Status a 20 Table 2-8: Self-Rated Health by Pain Status a 21 Table 2-9: Standardised Mental Health Score of 60 Or Morea 22 Table 2-10: Lost Work Days and ... provided their approval for this use of the material. The high price of pain While every effort has been made to ensure the accuracy of this document, the uncertain nature of economic data, forecasting...

Ngày tải lên: 20/02/2014, 20:20

... variants of TPP II ( i.e. rat, mouse, fruit fly, Arabidopsis thaliana, Caenorhabdit is elegans and Schizosaccharo myces pombe), and at least three human EST-clones covering this area (GenBank a ... open bars, respectively. The fragment originating from the rat gene is indicated by a hatched bar. The activity in cell extracts of stable transformants was measured as described in Materials and ... standard assay and the i mmunoreactivity was detected by Western blot analysis and quantitated as described i n Materials and methods. Open and filled circles indicate the activity, and open and...

Ngày tải lên: 21/02/2014, 15:20

... glycosyl- transferase elucidate catalysis in the alpha-amylase family. Nat. Struct. Biol. 6, 432–436. 16. Terwisscha van Scheltinga, A. C., Armand, S., Kalk, K.H., Isogai, A. , Henrissat, B. & Dijkstra, ... Plesniak, L .A. , Ziser, L., Wakarchuk, W.W. & Withers, S.G. (1996) The pKa of the general acid/base carboxyl group of a glycosidase cycles during catalysis: a 13C-NMR study of Bacillus circulans ... crystallo- graphy that replacement of the Asp142 analogue by alanine in other family 18 chitinases puts the N-acetyl group in a conformation which is not favourable for nucleophilic attack on the...

Ngày tải lên: 07/03/2014, 14:20

... that the partially flattened hierarchical hid- den Markov model (PFHHMM) can assign propo- sitions to language texts (grammar parsing) at least as accurately as the HMM. This is assignment is a task ... .)) where the part -of- speech tag associated with each word is attached with an underscore, the clause in- formation is identified by the S symbol and the chunk information is identified by the rest of the symbols ... structure of an HHMM The models discussed here are evaluated by applying them to natural language tasks based on CoNLL-2004 1 and a sub-corpus of the Lancaster Treebank 2 . Keywords: information extraction,...

Ngày tải lên: 08/03/2014, 02:21

... literature. Evaluations of subsite maps of rice and barley a- amylases are thus also presented. MATERIALS AND METHODS SUMA software: subsite mapping of amylases This software calculates the apparent binding ... Mapping of a- Amylases) is freely available for research and educational purposes via the Internet (E-mail: gyemant@tigris.klte.hu). The advantages of this program are demonstrated through a- amylases ... & Thoma developed a method based on the product analysis. Both methods have their advantages and disadvantages. The product analysis method of Allen & Thoma is suitable for endo-amylases,...

Ngày tải lên: 08/03/2014, 09:20

... Murakawa, M., Takahashi, S., Tsubuki, S., Kawashima, S., Sakamaki, K. & Yonehara, S. (1998) Purification, molecular cloning, and characterization of TRP32, a novel thioredoxin-related mammalian ... than the latter. As the C-terminal region is rich in acidic amino acids, if it does have some interaction with the N-terminal domain, the mechanism of regulating the catalytic activity may be ... model, then refined smoothly in alternating steps of automatic adjustment with CNS and manual adjustment with the program O [34]. The final model has a final R-factor of 0.222 with a free R-factor of 0.253....

Ngày tải lên: 08/03/2014, 22:20

... mechanism of mammalian ACL [23], the final step of the reaction can be assumed to be the nucleophilic attack of CoA to the phosphorylated carbonyl carbon of citryl phosphate, and the cleavage of the ... structures of AclA and AclB with that of the mammalian enzyme, we found that AclA and AclB Correspondence to T. Imanaka, Department of Synthetic Chemistry and Biological Chemistry, Graduate School of ... subunit. Another function of AclB was found to be stabilization of the enzyme, as AclB prevented the degradation of AclA that was otherwise observed in the absence of AclB. After the phosphorylation,...

Ngày tải lên: 08/03/2014, 22:20

... 2002 Academia Sinica (Taipei, Taiwan) for assistance in the chemical synthesis of inhibitor analogues. We thank Dr Yuch-Cheng Jean of the Synchrotron Radiation Research Center (Hsinchu, Taiwan) and ... 226530014, E-mail: shchiou@gate.sinica.edu.tw and A. H J. Wang, Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei, Taiwan. Fax: +886 227882043, E-mail: ahjwang@gate.sinica.edu.tw Abbreviations: ... inhibitors, as well as providing an initial model for the rational design of inhibitors against disease-related ADAMs and MMPs, such as TACE. By solid-phase peptide synthesis, we have prepared these...

Ngày tải lên: 08/03/2014, 23:20