the physiology of the respiratory mechanism
Understanding cofactor f420 dependent mechanism(s) in the activation of bicycle nitroimidazoles and in the physiology of mycobacterium tuberculosis
Ngày tải lên :
09/09/2015, 10:17
... the physiologic significance of their activating machinery in Mtb form the basis for the research work described in this thesis Before detailing the objectives of the thesis in section 1.6, the ... consequently there is a recruitment of several other components of the immune system that result in the remodelling of the infection site (Ulrichs and Kaufmann, 2006;Flynn and Chan, 2005) The result is the ... detection of F 420H2 oxidation was not feasible since the rate of Ddn catalyzed F420H2 oxidation was comparable to the spontaneous re-oxidation of F420H2 in the absence of the enzyme Therefore μM of...
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Study on the Photolytic Mechanisms of Red 141 Dye Wastewaters with an 185nm Vacuum-UV lamp
Ngày tải lên :
05/09/2013, 09:08
... considerable disadvantage of the majority of the degradation processes introduced above is the need to add external agents into the aqueous medium In such situation, the effectiveness of the processes relies ... Each run of the experiments in this work was replicated twice The standard deviations of the concentration of Red 141 was analyzed to be ±0.1 mg/l, respectively The concentration of H2O2 in the aqueous ... the excitement of O2 OH· indirect oxidation generated from the excitement of H2O OH· indirect oxidation generated from the excitement of H2O2 Figure The driving forces for the decomposition of...
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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf
Ngày tải lên :
14/02/2014, 22:20
... inhibition of the binding of the superoxide substrate and the other to product inhibition by hydrogen peroxide There are two mechanisms proposed for the reaction cycle of Mn ⁄ Fe-SOD: the 5-6-5 mechanism ... NE2 of His31, bound to the metal, in the company of a water oxygen, from the apical positions The manga˚ nese was only 0.06 A out of the equatorial plane (Table 3) The angles around the metal cofactor ... [26] These findings lead to the hypothesis that Fe-bound ApeSOD mimics the product-inhibited form and the shift of Tyr39 suppresses the release of the peroxide product This may be one of the reasons...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
Ngày tải lên :
19/02/2014, 16:20
... composed of residues 25–540 The overall structure of the model of IPU and the mutated residues in this study are shown in Fig To elucidate the mechanism of the substrate recognition of IPU, two ... a-(1fi4)-linked glucose units of pullulan (Fig 4A) IPU is an anomer-inverting enzyme Comparison of the active sites of the model of IPU and Dex49A The active site structures of the model of IPU (Fig 4A) and ... compare the mechanism of hydrolysis of IPU and other GH family 49 dextranases, the anomer configuration of the hydrolysate of IPU was determined A polarimetric assay was carried out using panose as the...
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Tài liệu Báo cáo khoa học: Unraveling the catalytic mechanism of lactoperoxidase and myeloperoxidase A reflection on some controversial features Elena Ghibaudi and Enzo Laurenti docx
Ngày tải lên :
20/02/2014, 02:21
... each of these questions, we will propose a new hypothesis that describes an integrated vision of the catalytic mechanism of MPO and LPO A brief survey of the absorption features of MPO, LPO and their ... oxidize them [19] On the other hand, this fact does not represent proof of the presence of one or two oxidizing equivalents on the decay product of Cpd I The EPR spectrum of a spin-trapped radical ... presence of H2O2 alone, a non-physiological condition We believe that the absence of direct evidence of the radical in the presence of cosubstrates limits the validity of extrapolating the model...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
Ngày tải lên :
21/02/2014, 01:21
... one of the manganese ions This, together with the fact that the positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the ... affected by the mutation, as revealed by the conservation of the whole topology of the active site, would explain the essentially unaltered interaction of the D153N variant with the metallic cofactor ... indicating that the environment of tryptophan residues is essentially conserved in the mutant enzyme On the other hand, the absence of major differences in the CD spectra of the wild-type and...
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Aging of the Respiratory System: Impact on Pulmonary Function Tests and Adaptation to Exertion pdf
Ngày tải lên :
05/03/2014, 21:20
... elastic recoil of the lung (Fig 2) [9] The kyphotic curvature of the spine and the anteroposterior diameter of the chest increase with aging, thereby decreasing the curvature of the diaphragm ... static lung volumes are the elastic recoil of the chest wall and that of the lung parenchyma Loss of elastic recoil of the lung parenchyma and, to a lesser degree, decrease in respiratory muscle performance ... Conversely, there is a progressive decrease in vital capacity to approximately 75% of best values Because of the increased stiffness of the chest wall, the age-related diminished elastic recoil of the...
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Báo cáo khoa học: The catalytic mechanism of dye-decolorizing peroxidase DyP may require the swinging movement of an aspartic acid residue potx
Ngày tải lên :
06/03/2014, 00:20
... and CN, the position of the carbon atom of CN bound to the heme iron mimics the position of the proximal oxygen of H2O2 bound to the heme iron during compound I formation Therefore, the carbon ... the heme of the native is shown in white The position of the CN carbon mimics the position of the proximal oxygen of H2O2 Double-headed arrows show whether the distance between Asp171 and the virtual ... to the position characteristic of the native The side chain of the catalytic residue of DyP is not located just above the heme iron, unlike in other heme peroxidases Moreover, the side chain of...
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Báo cáo khoa học: Spectroscopic investigation of the reaction mechanism of CopB-B, the catalytic fragment from an archaeal thermophilic ATP-driven heavy metal transporter potx
Ngày tải lên :
07/03/2014, 00:20
... series of uorescence titrations of mant-ATP to CopB-B in the presence of various competitor concentrations [L0], the binding constant of the nucleotide can be determined from the slope of app the ... (4) b For mant-ATP in the absence of competitor, the value for Kdiss is given GTP showed afnities in the same order of magnitude On the other hand, ADP, the product of the ATPase reaction, bound ... (bottom) The hatched zones indicate the loss of c-ATP in the precursor state and the formation of inorganic phosphate at the nal stage of the phosphatase reaction k)2 =1.0 ã 10)4 s)1 (Table 3) The...
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Báo cáo khoa học: Transthyretin and familial amyloidotic polyneuropathy Recent progress in understanding the molecular mechanism of neurodegeneration pdf
Ngày tải lên :
07/03/2014, 10:20
... strategy if the concentration of the oligomeric species were also decreased Mechanism of neurotoxicity in FAP: the lesson from other amyloidoses A number of studies have examined the mechanism of neurotoxicity ... the location of amyloidogenic mutations and position of b-strands (A) The structure of the polypeptide backbone of the two chains (purple and blue) is shown along with the location of the N-terminus ... tissue and that the distribution of TTR deposition reflects the presence of these endogenous factors In the case of the Ab protein of Alzheimer’s disease, a number of proteins and factors (pathological...
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Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
Ngày tải lên :
07/03/2014, 15:20
... all of the residues of the catalytic mechanism are present in the neighborhood of the catalytic site, but they are not ordered correctly in the exotoxin alone CaM binding folds parts of the regulatory ... are, respectively, the values of the center and the normalized amplitude of each lifetime peak c Ii are the values of the partial P intensity of the ith component Ii ¼ aisi/ d The mean lifetime ... activation mechanism Recently, the resolution of the 3D structure of the AC exotoxin of B anthracis and of its complex with CaM led to the proposal of a different CaM-dependent regulatory mechanism, ...
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Báo cáo Y học: Kinetic and biochemical analyses on the reaction mechanism of a bacterial ATP-citrate lyase ppt
Ngày tải lên :
08/03/2014, 22:20
... with the addition of citrate, suggesting the transfer of the labeled phosphate from the enzyme to citrate (data not shown) The results support an ordered mechanism of the enzyme reaction where the ... on the left of the panel The asterisk indicates the degradation product of AclA described in the text residue Our results suggest that the AclB subunit contributes in enhancing the efciency of ... contribution of AclB, as well as the b subunit of Ec-SCS, in the catalytic process seems to be assistance of the nucleotide binding to and phosphorylation of the a subunit Another function of AclB...
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Báo cáo khoa học: The role of Tyr71 in Streptomyces trypsin on the recognition mechanism of structural protein substrates ppt
Ngày tải lên :
16/03/2014, 00:20
... specificity and the conformation of the three-dimensional structures (A) The overall structure of SOT is portrayed using the Swiss-pdb viewer based on the crystal structure of SGT The key residue ... 400 nm The spectra of the proteins, an average of four scans, were corrected by subtracting the spectra of the corresponding background media without protein Substrate recognition mechanism of Streptomyces ... are shown The recombination sites of the chimeras are boxed The names of the chimeras are indicated in bold capital letters The catalytic residues are indicated in red Identification of amino acid...
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Báo cáo khoa học: Untangling the folding mechanism of the 52-knotted protein UCH-L3 potx
Ngày tải lên :
16/03/2014, 01:20
... are the spectroscopic signals of the native and denatured states in the absence of denaturant and bN and bD are the slopes of the native and denatured baselines and [D] is the concentration of ... resistant to unfolding and thereby minimize the risk of degradation by the 26S proteasome [3,25] Given the current lack of knowledge regarding both the stability and folding of the 52-knotted ubiquitin ... [D]50%, H2 O the midpoint of the unfolding transition;DGDÀN , the difference in free energy between the native and denatured states in the absence of denaturant; and mD)N, a measure of the change...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
Ngày tải lên :
16/03/2014, 03:20
... hydrolysis of the 2¢ : 3¢-cyclophosphate group Additional interactions stabilize the initial intermediate of the reaction Following determination of the structure of the complex between the Kid ... evaluation of the available model on the interaction and cleavage of the RNA substrate and the Kid toxin is of interest in itself because it is the basis of important cellular roles of this toxin ... to test the formation of a stable heterooctameric Kid2–Kis2–Kid2–Kis2 complex on the parD promoter [9] We further analysed the effects of the mutations on the co-regulatory activity of the toxin,...
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A study in the growth mechanism of silicon nanowires with or without metal catalyst
Ngày tải lên :
16/03/2014, 15:09
... with the catalyst This results in formation of only a few of SiNWs The reaction temperature is usually decided with the consideration of the catalyst type According to the binary phase diagram of ... [19] Therefore, the diameter and location of the SiNW are determined by the features of the catalyst particle [20] Thus, it is important to control the catalyst size distribution and the delicate ... and discussion 3.1 Metal-assisted growth of SiNWs A basic aspect of the VLS mechanism is the metal particle acting as a catalyst for the anisotropic growth of SiNW with a crystalline structure A...
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Disease of the Respiratory system in Children ppt
Ngày tải lên :
22/03/2014, 09:20
... Introduction The disease of respiratory system is one of the most frequent reasons for hospitalization of infants and children Basic knowledge of the development and functions of respiratory system ... infection →develop obstruction The right bronchus →direct continuation of the trachea; The left bronchus spreads out from the lateral surface of trachea; So foreign body →often aspirated into right ... respiration The younger the child, the more rapid the respiration is The metabolism and oxygen requirement of infants →high, but respiratory volume is limited →have to increase frequency of respiration...
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Báo cáo khoa học: Identification and characterization of important residues in the catalytic mechanism of CMP-Neu5Ac synthetase from Neisseria meningitidis potx
Ngày tải lên :
22/03/2014, 21:21
... the O9 atom, lies at the base of the binding pocket formed by the backbone atoms of Asn175 [12,18] (Fig 4) Although site-directed mutations cannot change the nature of the backbone, we nevertheless ... in the presence of a fixed concentration of CTP of 0.154 mm, it was not possible to saturate the enzyme, and only an estimate of the kcat ⁄ Km for Neu5Ac could be made from the gradient of the ... treatment of the D211A mutant increased the activity by more than four-fold Increasing the concentration of Mg2+ had little effect on the D209A mutant These differences perhaps reflect the nature of the...
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Báo cáo khoa học: Heterologous expression of a serine carboxypeptidase-like acyltransferase and characterization of the kinetic mechanism potx
Ngày tải lên :
23/03/2014, 07:20
... bi-bi mechanism Investigation of the substrate specificity of AtSMT towards l-malate revealed structural features required for the interaction of the acyl acceptor with the enzyme The lack of enzymatic ... revealed the codon usage of the Arabidopsis gene as well as the nature of the signal peptide and the sequence motif around the translation start as critical parameters for efficient expression of AtSMT ... extension of the SMT sequence with both the ER retention signal and the 6xHis tag led to severe reduction of SMT activity, thus revealing the requirement of a native C-terminus Kinetic studies The...
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Báo cáo khoa học: Understanding the complex mechanisms of b2-microglobulin amyloid assembly potx
Ngày tải lên :
28/03/2014, 22:20
... analysed the folding and unfolding kinetics of b2m under an array of conditions, including analysis of the folding mechanism of the variant P32G Using global analysis of the resulting kinetic data, the ... residues in the BC- and FGloops, the D-strand and the N-terminal region of the protein that presumably arise from the isomerization of Pro32 and subsequent partial unfolding of the protein [67] These ... studies of the folding and unfolding mechanism of wild-type b2m, combined with mutagenesis of the sequence, demonstrated that the transition between the slow folding intermediate I2 and the native...
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