the nitrate nitrite nitric oxide pathway in traditional herbal medicine for heart disease
Báo cáo y học: " The anti-inflammatory effects of the tellurium redox modulating compound, AS101, are associated with regulation of NFB signaling pathway and nitric oxide induction in macrophages" doc
Ngày tải lên :
11/08/2014, 08:22
... 0.05 vs LPS binding at the NFkB consensus site in the iNOS promotor following LPS induction Discussion In the present study, we attempted to elucidate the antiinflammatory effects of the tellurium ... vs h Furthermore, AS101 treatment attenuated p50 subunit DNA-binding abilities in the iNOS promoter Furthermore, the secretion of the inflammatory cytokine IL-6, regulated by the NFkB pathway, ... cells To address whether inhibition of iNOS was paralleled by a reduction in NO release, NO production was determined in the form of nitrite in culture supernatants using the Griess reagent Unstimulated...
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Báo cáo khoa học: Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts docx
Ngày tải lên :
07/03/2014, 05:20
... an ink spot Argininosuccinate is the immediate precursor to arginine in the urea cycle, and is converted to arginine and fumarate by argininosuccinate lyase (ASL; EC 4.3.2.1; Fig 5) Argininosuccinate ... in human liver [53], whereas the reported Km values for the reverse reaction are 5.3 mm for fumarate and 3.0 mm for arginine [53] If argininosuccinate synthesis is being catalyzed by ASL in the ... terminated immediately after the addition of [14C]arginine) The average c.p.m for the control reaction was approximately 1800 Reactions were performed using the complete, initial buffer containing...
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Báo cáo khoa học: "Immunohistochemical study of constitutive neuronal and inducible nitric oxide synthase in the central nervous system of goat with natural listeriosis" potx
Ngày tải lên :
07/08/2014, 14:23
... proportion of the neurons in the brainstem and the staining pattern was similar to those of control brains with nonneurological diseases In the microabscesses, iNOS was recognized in inflammatory ... and iNOS in neurons increased when bacterial encephalitis occurred This is the first study of NOS expression in the goat brain with listeriosis The involvement of iNOS in bovine and caprine listeriosis ... of nNOS and iNOS in the nonneurological control brain Both nNOS and iNOS immunoreactivity was recognized in the brain of control goats No inflammatory cells were found in the brain sections of...
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Báo cáo y học: "Gender-based reciprocal expression of transforming growth factor-β1 and the inducible nitric oxide synthase in a rat model of cyclophosphamide-induced cystitis" ppsx
Ngày tải lên :
11/08/2014, 08:22
... beginning of day and then stabilize for the remainder of the day Values at baseline in female rats (empty black dot) did not change over the course of the day The levels of NO products in urine ... exhibited the most intense green stain for iNOS in the urothelium as compared to the other groups The immunocytochemical expression of iNOS was much lower in control male rats (Fig 6C) The bladders ... induction of healing and in the bladder Our results therefore argue in favor of evaluating urinary TGF-β1 in IC patients in order to assess disease progression, and may point to novel areas for...
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The role of nitric oxide and other gaseous mediators in cardiovascular disease models; emphasis on septic shock
Ngày tải lên :
14/09/2015, 14:16
... of endothelial cell activates Ca2+ influx, with the increase in intracellular Ca2+ activating through calmodulin the endothelial nitric oxide synthase (eNOS), an oxygenase using L-arginine and ... (CLP)-induced septic shock model Induction of sepsis is intrinsically more challenging than inducing endotoxemia since the immune challenge is episodic in nature Some of the guidelines for progressive ... model, for the third part of the project (Chapter 7) In the following introductory chapters, we shall provide background information concerning, (i) the different animal models of producing septic/endotoxic...
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Dysfunctional signaling pathway for nitric oxide production in endothelial cells chronically exposed to high glucose or high fatty acids
Ngày tải lên :
15/09/2015, 17:09
... least three distinct isoforms of nitric oxide synthase in the body, i.e endothelial nitric oxide synthase (eNOS), immune or inducible nitric oxide synthase (iNOS) and neuronal nitric oxide synthase ... NG-nitro-L-arginine methylester 10 NADH nicotinamide adenine dinucleotide NADPH nicotinamide adenine dinucleotide phosphate NO nitric oxide nNOS neuronal nitric oxide synthase isoform PC phosphatidylcholine ... mechanism remains inconclusive Since nitric oxide (NO) production from the endothelium is the major regulating factor in this event, the aim of this work was to extensively investigate the effects...
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The role of nitric oxide and prostaglandin e2 in prolonged hemorrhagic shock 2
Ngày tải lên :
16/09/2015, 15:54
... sympathetic nervous system activation, the release of epinephrine and norepinephrine from the adrenal medulla reinforces the actions of direct sympathetic nervous system innervations of the heart ... significantly increased in control rats Lcitrulline is an indicator of nitric oxide (NO) synthesis Thus these findings indicate that NO production in these areas contributes to the hypotension ... numerous efforts and studies have aimed to evaluate the potential of NOS inhibitors in maintaining mean arterial blood pressure (MABP) and increasing the survivability of the shocked animals NOS inhibitors...
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The role of nitric oxide and prostaglandin e2 in prolonged hemorrhagic shock 1
Ngày tải lên :
16/09/2015, 15:54
... involved in neurological deficit These findings suggest that NO formation via iNOS activation may contribute to organ damage (brain) and that the iNOS inhibitor, AG, may be of interest as a therapeutic ... conscious rats There was increased brain nitrate/ nitrite production 24, 48 and 72 hours after prolonged hemorrhagic shock in saline-treated rats Also, there was an increased brain infarct volume ... 4.4.2 Hemotoxylin and eosin stain of Lungs 63 Figure 4.4.3 Hemotoxylin and eosin stain of Livers 64 Figure 4.4.4 Hemotoxylin and eosin stain of Cerebral cortex 65 Figure 4.5 Neurons in the cerebral...
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Tài liệu Báo cáo khoa học: Implication of the glutamine synthetase ⁄glutamate synthase pathway in conditioning the amino acid metabolism in bundle sheath and mesophyll cells of maize leaves doc
Ngày tải lên :
18/02/2014, 18:20
... compounds in the phloem exudates, amounting to 51% of the total amino acids in the light and 56% in the dark (Fig 7A) The total amount of alanine, serine and glycine was reduced from 33% in the light ... by the aminotransferases, which, in turn, consume the equivalent amount of glutamate in the reverse reactions to form aspartate and alanine for further amino acid interconversions Therefore, the ... al acid synthesis in these cells, from which the amino acids are loaded to the phloem for the grain In the phloem sap, glutamine is the preferred nitrogen carrier rather than asparagine (Table...
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Báo cáo khóa học: SUT2 is a novel multicopy suppressor of low activity of the cAMP/protein kinase A pathway in yeast docx
Ngày tải lên :
07/03/2014, 15:20
... of SUT2 then could, in analogy to its homologue SUT1, relieve this sterol precursor-starvation by increasing sterol-biosynthesis Therefore it will be interesting to determine if genes of the sterol ... grant from the Deutsche Forschungsgemeinschaft to W B (Ba415/24–1) We thank H U Mosch, ¨ Gottingen, for the gift of strains and plasmids We are also indebted ¨ to B Klebl, Martinsried, for the gift ... rescue the RAS1 RAS2-double deletion, suggesting that SUT2 either acts upstream or, alternatively, in a parallel pathway to RAS To further investigate the relation between SUT2 and the RAS/cAMP pathway...
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Báo cáo khoa học: Transport of L-arginine and nitric oxide formation in human platelets pdf
Ngày tải lên :
08/03/2014, 02:20
... present on the trans-side of the membrane Moreover system y+L is inhibited by L-leucine in the presence, but not in the absence of Na+ The small inhibition of L-arginine in ux by L-leucine in the absence ... transport of the cationic amino acids lysine, arginine and ornithine at the basolateral membrane of the polar epithelial cells in the intestine and renal tubules LPI is caused by mutations in the SLC7A7 ... and resuspended in pH 7.4 Hepes buffer in the absence (continuous lines) or in the presence (dotted lines) of 1.0 mM L-arginine The L-arginine efflux via system y+ (d) was determined as difference...
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Báo cáo khoa học: Kinetics of electron transfer from NADH to the Escherichia coli nitric oxide reductase flavorubredoxin pdf
Ngày tải lên :
16/03/2014, 11:20
... of the Rd centre in the whole enzyme The kinetics of FlRd reduction was investigated by anaerobically mixing the oxidized protein with FlRdreductase preincubated with excess NADH Also in the ... instead of FMN per monomer), pointing to partial loss of avin during the purication procedure or incomplete incorporation of avin during expression Electron transfer to E coli avorubredoxin for ... A), but the enzyme displays a homodimeric assembly in a head-to-tail conguration, bringing the FMN of one monomer in close proximity to the Fe-Fe site of the other monomer It is therefore likely,...
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Báo cáo khoa học: Cupiennin 1a, an antimicrobial peptide from the venom of the neotropical wandering spider Cupiennius salei, also inhibits the formation of nitric oxide by neuronal nitric oxide synthase pptx
Ngày tải lên :
23/03/2014, 09:21
... throughout the CaM sequence, including the C-terminal and N-terminal domains This means that a substantial change in conformation occurs for the regulatory protein upon binding of cupiennin 1a to ... [10,11,25,26], and therefore adversely affects binding of CaM to the Ca2+-CaM-binding domain of nNOS CaM is not only essential for the operation of nNOS and the other NOS isoforms, but is also the regulatory ... synthase (eNOS) [33] The second example is when the C-terminal lobe of Ca2+-CaM binds part of the target peptide This is shown in Fig 4B for the 20residue binding domain of the plasma membrane...
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báo cáo hóa học:" Role of the VEGF-Flt-1-FAK pathway in the pathogenesis of osteoclastic bone destruction of giant cell tumors of bone" pptx
Ngày tải lên :
20/06/2014, 04:20
... were initially decalcified for two weeks in an EDTA-containing buffer and embedded in paraffin The endogenous peroxidase activity was quenched by incubating the sections for an additional 30 in ... VEGF activates the VEGF-Flt-1-FAK pathway The activation of this signaling pathway might be involved in the migration of these cells into the lesion at the site of bone destruction in GCTs We recently ... OPCs to the neoplastic lesions In this study, we examined clinical GCT samples in order to determine the possible role of the VEGF-Flt-1-FAK pathway in the pathogenesis of bone destruction in GCTs...
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báo cáo hóa học:" Role of the VEGF-Flt-1-FAK pathway in the pathogenesis of osteoclastic bone destruction of giant cell tumors of bone" potx
Ngày tải lên :
20/06/2014, 07:20
... were initially decalcified for two weeks in an EDTA-containing buffer and embedded in paraffin The endogenous peroxidase activity was quenched by incubating the sections for an additional 30 in ... VEGF activates the VEGF-Flt-1-FAK pathway The activation of this signaling pathway might be involved in the migration of these cells into the lesion at the site of bone destruction in GCTs We recently ... OPCs to the neoplastic lesions In this study, we examined clinical GCT samples in order to determine the possible role of the VEGF-Flt-1-FAK pathway in the pathogenesis of bone destruction in GCTs...
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Báo cáo khoa học: "Enhanced expression of constitutive and inducible forms of nitric oxide synthase in autoimmune encephalomyelitis" pdf
Ngày tải lên :
07/08/2014, 14:22
... that the three isoforms of NOS including nNOS, eNOS, and iNOS were increased in the initiation of EAE and suggested that the brain cells including neurons and astrocytes are possible sources for ... by the inflammatory cells in the pathogenesis of EAE, as does iNOS in EAE [45] However, our study did not support the finding of EAE in iNOS knockout mice in which both nNOS and eNOS were not increased ... be involved in either the tissue destruction in traumatic brain injury [41] or in the survival of neuronal cells in vesicular stomatitis virus infections [24] Taken these dual effects of nNOS in...
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Báo cáo khoa học: " A novel b-glucan produced by Paenibacillus polymyxa JB115 induces nitric oxide production in RAW264.7 macrophages" docx
Ngày tải lên :
07/08/2014, 23:22
... to the NO production during macrophage activation Polymyxin B has shown inhibitory effects on the lethal endotoxic activity of LPS in vivo and on the in vitro mitogenic activity of LPS by forming ... with the lipid A of LPS [21] Therefore, this study also investigated the effects of polymyxin B on the activity of βglucan and LPS in order to exclude any possible contamination due to endotoxins ... Polysaccharides isolated from Phellinus baumii stimulate murine splenocyte proliferation and inhibit the lipopolysaccharide-induced nitric oxide production in RAW264.7 murine macrophages World J Microbiol...
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Báo cáo y học: "κ The TRAF6-NFκB signaling pathway in autoimmunity: not just inflammation" ppsx
Ngày tải lên :
09/08/2014, 06:23
... activation in the periphery and in the thymus, the immune system must balance antigen presentation and proinflammatory outcomes in the periphery in response to pathogens and other environmental inflammatory ... cells The two main NFκB activation pathways are marked in blue IL, interleukin; IRAK, IL-1 receptorassociated kinase; MAPK, mitogen-activated protein kinase; mTEC, medullary thymic epithelial cell; ... deficiencies of the TRAF6-NFκB pathway, and regulation of oxidation/reduction, will continue to be found in human autoimmune diseases, and that the thymus is implicated in its pathogenesis Competing interests...
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Báo cáo y học: "Ex vivo effects of flavonoids extracted from Artemisia herba alba on cytokines and nitric oxide production in Algerian patients with Adamantiades-Behcet''''s disease" pptx
Ngày tải lên :
11/08/2014, 03:20
... suggested that the overexpression of either inducible NO and proinflammatory cytokines might be intimately involved in the pathogenesis and the evolution of ABD [12, 42] An increase in the concentration ... showed the pivotal role of IFN- in pathophysiology of ABD particularly via the NO pathway [46] There is an increasing interest in herbal medications especially for diseases like ABD [47, 48] The ... highly inhibited the production of the proinflammatory cytokine IL-12 in ABD patients PBMC The mechanism involved remains to be clarified Furthermore, in our study we reported that the inhibitory...
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