the mouse incisor as an emerging model for tooth renewal

... dedication and passion for research I would like to thank the students and staff of BIOMAT Centre, past and present, for their help and the pleasure of their company: Chee Kong, Fenghao, Kay Siang, ... Solution to Organ Shortage Organ transplantation has become increasingly common in the past 30 years and has saved many lives, while having improved the quality of lives of thousands of other patients ... quickly implanted, as there is no need for extended in vitro culture for maturation Furthermore, the substrate cues can be introduced to the material, or the material can be loaded with relevant biomolecules,...

Ngày tải lên: 14/09/2015, 08:49

... cultured in the same medium, fibroblastic cells quickly grew out from fetal liver mononuclear cells, and failed to express endothelial phenotype 122 Chapter Isolation and Characterisation Of Vascular ... Chapter Isolation and Characterisation Of Vascular Progenitors Figure 4-3: Primary culture of haemopoietic tissue in EGM Following culture in endothelial cell media, outgrowth colonies ... from cord blood (a, b) In contrast, outgrowth colonies from fetal blood (c) were heterogeneous in nature, adopting morphologies ranging from cobblestone, fibroblastic to spindle (d - f) 123 ...

Ngày tải lên: 14/09/2015, 08:49

... vWF was sporadically expressed on the cells A functional assay of acLDL uptake was performed to confirm the endothelial nature of the cells In contrast to the immunostaining results, acLDL was ... week, I transferred a portion of the cells to semi-solid cultures for haematopoietic colony forming assays as an assessment of the retention of haematopoietic character following expansion As seen ... generate endothelial progeny, I assayed for the expression of various endothelial markers, namely CD31, CD144, VEGFR2, as well as vWF The staining profile was compared with FB-EPC, as well as HUVE...

Ngày tải lên: 14/09/2015, 08:49

... Glow Discharge (RFGD) Plasma 5.2.1.4 Assessment of tensile properties The choice of the plasma-based method to modify the µXPCL surface was made on the basis of its advantageous low depth of penetration ... goat anti -mouse secondary antibody The label was conjugated onto activated films, and fluorescence 5.3.4 Conjugation Of Monoclonal CD34 Antibody Antibodies have previously been proposed for use as ... Furthermore, the cross-links may occur at or near the active site, obscuring the ability of the antibody to identify the cell antigen In my experiments, I had used titration methods to assess the...

Ngày tải lên: 14/09/2015, 08:49

... nanocarriers for cancer therapy Nanocarriers can offer many advantages over free drugs They: For rapid and effective clinical translation, the nanocarrier should: • • • • • • • • • protect the ... reduced toxicity and enhanced anti-metastatic effects in two different mouse tumour models, emphasizing the advantages of a mechanism-based design for targeted nanocarriers84 Organic nanoparticles ... radiation and heat up the surrounding cancer tissue Scattering nanoshells, on the other hand, are desirable as contrast agents for imaging applications Recently, a cancer therapy was developed based...

Ngày tải lên: 28/05/2014, 15:07

... membrane Pagliarini and Xu [10] therefore asked whether the metastatic behavior of RasV12scrib- cells is due to loss of cell polarity They found that lethal(2) giant larvae, discs large and other ... Mutant cell clones were then analyzed for cell proliferation and for invasion of distant larval tissues RasV12scrib- mutant cells overproliferate and form secondary tumors in the ventral nerve cord, ... aspects of human cancer and metastasis may have parallels in flies, we can expect many exciting new discoveries to be made using flies as a model to study metastasis reviews To test whether promotion...

Ngày tải lên: 09/08/2014, 20:20

... distributions [7] As the main resistance to heat-transfer can be found in the air on the dry face of the system, the advantage of the higher thermal conductance of metals than that of plastics is negligible ... In the last stages of the tunnel there will be no mass-exchange because Relative air Humidity (RH) is 100%, and heat exchange neither, as the air and water temperature are the same Thus, these ... included in the comfort area defined by ASHRAE (Figure 30) As well as the other two cases, cooling is performed as a consequence of an increase in the humidity level, though in this case it is...

Ngày tải lên: 05/09/2013, 16:10

... estimate of the pdf of the interference power, for different values of the shadowing standard deviation, sdB Based on these estimates, we then propose an analytical statistical model of the interference ... histograms and the proposed Burr-based distributions closely match for the whole range of sdB However, care must be taken in defining the range of gains for which our model is valid And indeed, the ... approximated analytical expression for the pdf and the cumulative distribution function (cdf) of the intercell interference power; • We propose a semi-analytical method for the estimation of the pdf of the...

Ngày tải lên: 21/06/2014, 00:20

... than in the sigma B mutant in stationary phase Of the 67 non-coding RNA elements (ncRNAs) they report as transcribed in stationary phase, 60 had already been described by other investigators and ... including the stationary-phase genes Over the past decade, the Listeria sigma B regulon, along with the regulons for other transcription regulatory factors Cossart and Archambaud: Journal of ... incompletely translated proteins for degradation and releases stalled ribosomes, while 6S RNA as shown in E coli mimics an open promoter to bind and sequester the sigma70-containing polymerase, inhibiting...

Ngày tải lên: 06/08/2014, 19:21

... technical assistance and Marina Freudenberg for providing TLR-2-/- and TLR-4def mice We also thank Thomas Boehm for valuable suggestions and for reading the manuscript critically This work was supported ... both for spirochetal and for third-party antigen can expand and secrete pro-inflammatory cytokines in infected tissue, thereby contributing to the disease progress Engagement of TLR-2 and other ... Germany; µM) and Omniscript RT (Qiagen, Hilden, Germany; U) Table The cDNA obtained was used as a template for real-time quantitative polymerase chain reaction, which was performed with the LC FastStart...

Ngày tải lên: 09/08/2014, 01:23

... American Society of Anesthesiologists Task Force on Blood Component Therapy: Practice guidelines for blood component therapy: a report by the American Society of Anesthesiologists Task Force ... occurred for several reasons, such as the age of the patients enrolled in the two studies, and the type and location of the surgery undergone In addition, the timing of the administration in the two ... provide an accurate reflection of the condition of the patient because of the time required to obtain the results In many cases, samples taken for analysis are rewarmed to 37°C before assay, and...

Ngày tải lên: 13/08/2014, 03:20

... listen and read a transcript or get instant feedback on what they have done (vi) Learners can access authentic websites They can plan out their own use of web-based materials in their own time Then ... conducted as a quasi-experimental design Three hypotheses were set up The first hypothesis stated in the research questions, the second one was the null hypothesis- there is no difference between the ... posttest scores of the experimental group and the control group Only one the alternative hypothesis was set up for the study: the pretest score of the experimental group and the control group...

Ngày tải lên: 04/08/2015, 09:41

... or ferrous salts, and studied for the performance and the mechanism in heavy metal removal (Kanel et al., 2005; Kanel et al., 2006; Li and Zhang, 2006; Li and Zhang, 2007) Kanel et al (2005, ... examined and their adsorption performance will be investigated Further improvement in the properties and performances of the prepared P4VP nanoparticles will be attempted The separation and recovery ... feasibility and effectiveness 1.3 Organization of the Thesis The contents of this thesis revolve around and branch out from the central theme of the doctoral research project, i.e the preparation and...

Ngày tải lên: 10/09/2015, 15:54

... all models, and for the LM we use an interpolated Kneser-Ney 5-gram model For GIZA ++, we use the standard training regimen up to Model 4, and combine alignments with grow-diag-final-and For the ... probability of one phrase given the other, which incorporates word-based alignment information as prior knowledge in the phrase translation probability We take the geometric mean5 of the Model probabilities ... varied the size of the parallel corpus for the Japanese-English task from 50k to 400k sen- For future work, we plan to refine HLEN to use a more appropriate model of phrase length than the uniform...

Ngày tải lên: 20/02/2014, 04:20

... that the correct rate of recognition can be as high as 98.3% This indicates that the language processor based on the integration of the unification grammar and the Markov language model can in ... to form a complete speech recognition system The language processor consists of a language model and a parser The language model properly integrates the unification grammar and the Markov language ... shared by many different sentence hypotheses The Laneua~e Model The goal of the language model is to participate in the selection of candidate constituents for a sentence to be identified The proposed...

Ngày tải lên: 08/03/2014, 07:20

... on the structure and function of yeast enolase will be similar to their effects on human bb-enolase Figure shows the basic fold of yeast enolase, including the location of the active site and ... (Table 1) The s20,w value for the G376E variant was measured at four protein concentrations and the Kd value for this variant was calculated; Kd was increased by a factor of 103 (Table 1) Thermal ... phenylalanine The aromatic CD spectra of the dimeric forms of the wild-type enolase and the W56F variant are shown in Fig 2B The major change in the CD spectrum of the wild-type enolase, which...

Ngày tải lên: 16/03/2014, 06:20

... Glaubitz and Andreas Engel for valuable discussions, and we thank Walter Rosenthal for the cDNA of human ETB We further ´ thank Robert Tampe and Katrin Schulze for their help with SPR analysis The ... in the NTD and C131 in the first cytoplasmic loop C1 These data are in agreement with the above-mentioned findings, and they further define TMS1 as a central determinant for ET-1 binding On the basis ... environments and without the need for membrane reconstitution Most recently, a modified assay that employs the detergent-solubilized neurotensin receptor as the analyte has been described [35], and we...

Ngày tải lên: 16/03/2014, 10:20

... of the amplitude and phase (in degrees) for a given re¯ection, with the z* value given by the tilt angle and the angle between the tilt axis and a* The trend of the data for the continuous transform ... Coomassie staining The left lane shows band a and the right lane band c Molecular mass markers are indicated on the left of the panel Tris/urea-treated membranes, this band was also observed, ... axis The transmembrane helices of the accessory polypeptides CP47 and CP43 can not be readily identi®ed in the higher plant map, however, as < 50% of the mass of these subunits is contained in the...

Ngày tải lên: 17/03/2014, 17:20

... proteins Lane 1, anti-(H-PsbP); lane 2, anti-(H-PsbQ); lane 3, anti-(G-PsbQ); lane 4, anti-(R-PsbQ¢); lane 5, anti-(R-PsbV); lane 6, anti-(R-PsbU); lane 7, anti-(C-PsbU) A D Fig Reactivities of the ... in the cyanelle genome suggested that this gene has been transferred to the nuclear genome in this organism, as in the case of red algae On the other hand, the failure of cross-reaction with anti-(C-PsbU) ... their extrinsic proteins Lane 1, anti-(H-PsbP); lane 2, anti-(H-PsbQ); lane 3, anti(G-PsbQ); lane 4, anti-(R-PsbQ¢); lane 5, anti-(R-PsbV); lane 6, anti(R-PsbU); lane 7, anti-(C-PsbU) C caldarium...

Ngày tải lên: 23/03/2014, 15:21

... TreeBank data The correct attachment was chosen 72% of the time on the 500-phrase development corpus from the WSJ TreeBank Because it is a forced binary decision, there are no measurements for ... to the CP For example, whereas ( n l ce n3) (n3 cc nl), (v p n) ~ (n p v) In other words, there is no reason to make the distinction between "dog and cat" and "cat and dog." Modifying the model ... p(truelnl) = The terms p(nl) and p(n2) are independent of the attachment and need not be computed The other two terms are more problematic Because the training phrases are unambiguous and of the form...

Ngày tải lên: 23/03/2014, 19:20