the master transcription factor for osteoclastogenesis

... flavonoid biosynthesis are under the transcriptional control of different MYBs proteins [15-21] Among them, two MYB transcription factors, VvMYB5a and VvMYB5b, contribute to the transcriptional ... (DBD), appeared likely to modify the interaction with the DNA backbone and the protein activity by disrupting the ternary structure of the transcription factor itself The R69L mutation reduces VvMYB5b ... amino acid change on both the dimerization and the DNA binding properties of a grapevine MYB transcription factor These two functions appear related, as the conformation of the R2R3 domain, that...

Ngày tải lên: 11/08/2014, 11:21

... answer these questions To this end, we explored, for the first time, the human transcription factor network (HTFN) obtained from the protein–protein interaction information available in the TRANSFAC ... functionality and phylogeny However, the nature of the connections between these factors needs to be understood at the level of the protein domain The global properties of the HTFN topology are partially ... by the tiny lines in the topological overlap matrix The algorithm weights the (topological) association of any node to the others, and it is possible to build a dendro- Fig Distributions for...

Ngày tải lên: 19/02/2014, 07:20

... transcription factors as the mutation of the CRE site within the COX-2 promoter or the expression of a dominant negative mutant of CREB reduces inducibility of the COX-2 promoter [9,12–14] The transcription ... with high efficiency, they are not suitable for assessing the effects of the dominant negative ESE-1 on endogenous COX-2 mRNA levels Therefore, we evaluated the effects of the dominant-negative ... coactivators and other cofactors needed for transactivation of ESE-1, thereby depriving intact ESE-1 of its factors needed for transactivation DN1 and DN2 were cloned into adenovirus vectors and the expression...

Ngày tải lên: 19/02/2014, 17:20

... binding to the His6-TnrA surface, the analyte was preincubated for on ice with the various effector molecules as indicated, and was then injected into the sensor chip For novel reloading of the nitrilotriacetic ... PII trimer The upper panel shows the raw data in the form of the heat effect during the titration of 25 lM GlnK solution (trimer concentration) with ATP (titration from 2.1 to 73.5 lM) The lower ... lower panels show the binding isotherm and the best-fit curve according to the three sequential binding sites model BIAcore analysis of the GS–TnrA interaction The results of the immunoprecipitation...

Ngày tải lên: 06/03/2014, 00:21

... to the plasma membrane The dominant-negative form (Akt-K179M) contains a methionine for lysine substitution at residue 179 abolishing Akt kinase activity The wildtype form (Akt-WT) contains the ... deliverable to the organ in question In this study, we have provided direct evidence of HO-1 induction by insulin through the PI3K ⁄ Akt cascade and the Nrf2 transcription factor in both transformed ... transcription factor- 1 (HSF-1), hypoxia-inducible factor- 1 (HIF-1) and NF-E2-related factor (Nrf2) The PI3K ⁄ Akt pathway has been implicated in HSF-1 regulation by virtue of the repressive effects of the...

Ngày tải lên: 07/03/2014, 12:20

... cells We therefore investigated which transcription factors could regulate transcription of the transferrin gene To identify conserved sequences in the transferrin promoter, we first aligned the DNA ... the transcriptional activity of several classic transcription factors, including nuclear factor- jB and activator protein-1 [34,35] These observations raise the possibility that Ab modulates the ... (TF-luc mut3) and the forward primer given above for the TF-luc WT construct The amplified promoter sequences were cloned into the HindIII sites of the pGL4.12 basic vector and then FEBS Journal...

Ngày tải lên: 23/03/2014, 03:20

... measuring the whole area using the lateral view with the widest diameter from the dorsal to the ventral side of the immunostained pronephros The area included the pronephric tubules and the anterior ... different roles for the two HNF1 genes Whether differential properties of the two transcription factors are the cause of these differences, or rather the differential expression patterns, remains ... of the transcription factors, Pax8 and lim1, in Xenopus embryos led to the development of an abnormally large pronephros, and to the formation of ectopic pronephric tissue [44] As both these transcription...

Ngày tải lên: 23/03/2014, 13:20

... in play, and therefore, determine the selective combination of transcription factors involved and the regulatory pathways modulating transcription We have identified several Ets factors that specifically ... crucial for transcription of the human presenilin-1 gene J Biol Chem 274, 24297–24307 Pastorcic M & Das HK (2003) Ets transcription factors ER81 and Elk1 regulate the transcription of the human ... [15–17] The ets genes encode Regulation of the presenilin gene a family of transcription factors and most are transcriptional activators [18,19] They share a conserved 85-amino acid motif, the ETS...

Ngày tải lên: 30/03/2014, 09:20

... March 2002 Informed consent was obtained from the parents The study was performed in accordance with the principles of the revised declaration of Helsinki and has been approved by the ethics committee ... on the cheeks (35%) and nose (33%), less commonly on the forehead (20%) and rarely on the chin (8%) The hand (2%), forearm (1%) and neck (1%) were very rarely affected Fifteen per cent of the ... test) but the difference compared with control children was not significant (53%; P = 0.45, χ2 test) No significant differences between the groups were observed for the other SPTs, for the presence...

Ngày tải lên: 09/08/2014, 10:20

... 5'-TTGCAAGTTTGCTGCTGAAC-3' (95°C for 10 minutes followed by 40 to 50 cycles of 94°C for 15 s, 59°C for 20 s, 72°C for 30 s and 76°C for s (signals acquired)), whereas the primers for the promoter of mir-21 ... cells, a significant subset of transcription factor binding regions is extensively co-occupied by several different transcription factors to form multiple transcription- factor binding loci Our work ... show that, in the large majority of cases, where association between a transcription factor and genomic location is demonstrated, a classical consensus motif for the transcription factor cannot...

Ngày tải lên: 11/08/2014, 12:20

... are detrimental for the transcriptional synergism between these two factors [10] This synergism requires the independent binding of both factors to different cis-elements in the Hahsp17.6G1 promoter ... pointed to an additional DREB2 factor AtDREB2B, together with AtHSFA3, are the only two transcription factors among the genes that were specifically induced in thermotolerant lines of Arabidopsis ... temperature Therefore, the effect of HaDREB2 on seed longevity that is indicated by the BTA results in Figure was confirmed using the CDT procedures that we described for tobacco seeds [3] The results...

Ngày tải lên: 12/08/2014, 03:20

... We are also grateful to the Linquest Group at the Centre for Public Health at the County Council of Östergötland for providing access to the data for the reference group The present study is supported, ... only for age and sex then leads to a faulty interpretation of the HRQoL values among former ICU patients as their HRQoL may be assumed to be lower prior to the admittance to the ICU due to their ... studied in former ICU patients Key messages • The most important factor for the low HRQoL status reported long term by former ICU patients was their pre-existing diseases • ICU-related factors had...

Ngày tải lên: 13/08/2014, 20:21

... We report here the predominant role of the transcription factor specificity protein (Sp) in the expressional regulation of the human NOX4 gene Results GC-boxes are essential for transcriptional ... dramatically decreased by the deletion of GC-box1 The activity was further decreased by the deletion of GC-box2 (Fig 3B) As shown in Fig 3C, the transcriptional activity of the kb of the 5¢-flanking region ... decreased the intensity of the upper band for both GC-boxes and in both cell lines, suggesting that the binding of Sp1, detected by the upper band, was inhibited by the antibody On the other hand,...

Ngày tải lên: 29/03/2014, 00:20

... given promoter vary among the MMP family members, and these variations influence the regulation of these genes [37] The interaction of AP-1 with other transcription factors may also regulate ... and MMP-13 play dominant roles in the progression of RA and OA, making them prime targets for arthritis therapies Since the genes for both of these enzymes are transcriptionally activated by inflammatory ... have the unique ability to cleave the triple helix of collagen, thereby allowing the chains to unwind; the chains then become susceptible to further degradation by other MMPs Recently, MMP-8 (traditionally...

Ngày tải lên: 09/08/2014, 03:24

... log x for 0.1 ≤ fh(x) ≤ 0.9 The absolute slope of the regression line for the Hill plot yields a reliable estimate of the Hill coefficient Then, in the above range, response functions of the form ... different values for the protein half-life τp and the Hill coefficient h The expression curves of the mRNA and the protein are given by the dashed and the solid curves, respectively For better representation, ... of the delay system We simulated the delay system (3) for the different Hill coefficients calculated above Figures 3A and 3B display the simulation results for the parameters used in [8]: for...

Ngày tải lên: 13/08/2014, 23:20

Ngày tải lên: 01/11/2013, 11:20

... patterns for the different genes Although the interaction studies presented demonstrate that all BPM proteins can assemble with RAP2.4, and even provide strong evidence for the assembly of the transcription ... stained In the flower, we detected GUS expression exclusively in the pollen Siliques were stained at the base and at the tip, with overall very weak staining of the fused carpels In the root, the central ... µm 200 µm localization for the different BPM proteins, which might reflect their diverse biological roles in the cell In addition, they indicate that, except for BPM4, all other BPM proteins are...

Ngày tải lên: 18/02/2014, 06:20

... of the AP-2e gene were analyzed in detail to identify 2496 binding sites for known transcription factors that might regulate AP-2e expression Two potential binding sites for the transcription factor ... chondrocytes is regulated, the sequence of the AP-2e promoter was analyzed, and binding sites for the transcription factors AP-2a and Sox9 were identified Both transcription factors are known to play ... detected in the Sox9 ChIP DNA sample (D) EMSA to confirm the binding of Sox9 to the AP-2e promoter The contents of the reaction mixtures are marked above the image of the gel shift The Sox9 binding...

Ngày tải lên: 18/02/2014, 08:20

... antibodies for the transcription factor are available The assay is the first assay suitable for high-throughput measurements of transcription factor DNA interactions in biological samples Therefore, ... of the DNA binding of transcription factors in cell and tissue lysates Although we have developed the assay using HSF1, it can easily be adapted for the study of any transcription factor The ... microarrays [11], exist for investigating transcription factors and transcriptional regulation, but the main purpose of using these methods is not in quantifying transcription factor DNA binding Our...

Ngày tải lên: 18/02/2014, 14:20

... proposed to form helix IV However, in the absence of direct evidence supporting a structural interruption of the geometry of the recognition helix for either Antp or the TTF-1 HD, the anomalous ... conceivably a consequence of the overlap affecting the corresponding resonance Therefore, the experimental data of these three residues were not further considered for subsequent MF and RSDM analysis ... determinant for the NK-2 HD subfamily [31], within the flexible joint between the N-terminal and C-terminal moieties of the recognition helix The implication for DNA binding that may be envisaged from the...

Ngày tải lên: 18/02/2014, 16:20