the human protein protein interactome generation and analysis

... duplications expanded the metazoan and human mitochondrial proteome by the duplication of non-mitochondrial proteins and redirecting the newly arisen gene products to the mitochondria The two modes ... in these proteins the targeting signal is responsible for the mitochondrial localization, we examined whether its appearance in evolution coincides with the gene duplication, and thus whether the ... in the common ancestor of human and yeast, and a subsequent gain of mitochondrial localization We hypothesized that these proteins can constitute gains of mitochondrial localization in the human...

Ngày tải lên: 09/08/2014, 20:21

... the proteins are difficult to align because identity between the two proteins is low Nevertheless, the alignment in Fig shows that the overall similarity of the protein encoded by YBL046w to human ... determine whether the properties of R2 and the protein encoded by ORF YBL046w were also similar Bacterially expressed human GST–R2 was shown to interact with native Ppp4c and not with other protein ... Psy4p, the orthologue of R2, and the other was an unknown protein YLN201c, later named Psy2p because it was also found to be sensitive to cisplatin and oxaliplatin [1] Data from the proteome analysis...

Ngày tải lên: 07/03/2014, 12:20

... involved in the induction of apoptosis, including the 7a and 7b proteins [18,46,47] To analyze whether the deletion in ORF 7b had any influence on its Amino acid variability in ORF 7b and RT-PCR analysis ... constructed the infectious clone and conducted all experiments with recombinant viruses VK and EM designed and carried out the overexpression experiments and the reporter assays and/ or critically ... ORF 7a and ORF 7b [18] However, it is unclear to what extent either the ORF 7a or ORF 7b proteins, respectively, contribute to the effect It is also unclear to what degree the ORF 7b protein...

Ngày tải lên: 12/08/2014, 04:20

... involved in the induction of apoptosis, including the 7a and 7b proteins [18,46,47] To analyze whether the deletion in ORF 7b had any influence on its Amino acid variability in ORF 7b and RT-PCR analysis ... constructed the infectious clone and conducted all experiments with recombinant viruses VK and EM designed and carried out the overexpression experiments and the reporter assays and/ or critically ... ORF 7a and ORF 7b [18] However, it is unclear to what extent either the ORF 7a or ORF 7b proteins, respectively, contribute to the effect It is also unclear to what degree the ORF 7b protein...

Ngày tải lên: 12/08/2014, 04:20

... but in the HVS – the color perceived at a spatial location is influenced by the color of all the spatial locations in the field of view! Future issues for color image processing: use the human ... Austria Color image processing & analysis Human perception of color images (2) • The human color vision system: Primary light source Human eye Photoreceptors: Rodes and cones Reflected light Object’s ... [M(1,l’) M(2,p’) M(3,q’)]T ; otherwise: on the subset of (l’,p’,q’) candidates, select the one with largest s and i SSIP’08 – Vienna, Austria Color image processing & analysis 4.2 Color image filtering...

Ngày tải lên: 24/04/2014, 13:36

... contributions AS and AN conceived and designed the experiments AS performed the experiments with the help of CA and FT AS and HH analyzed the data CK, MvhH and HL contributed regents, materials and analysis ... for a function and making them priority candidates for genetic perturbation and phenotypic characterization Additionally, to gain an insight into the function of these ncRNAs and their transcriptional ... and other ncRNAs, such as the RNA component of RNase P (RPR1) and the U6 snRNA (SNR6) [59-61] To investigate the targets of the RNAP III machinery in C albicans, we performed ChIP-chip with the...

Ngày tải lên: 09/08/2014, 20:22

... Current map of inversions in the human genome The map of human inversions is still quite limited, and our understanding of the number of inversions, the size distribution and the frequency distribution ... fragments The ends of these fragments are then sequenced (fragments are depicted in blue and red, with the boxes at the ends showing the parts that are sequenced) The pairs of end-sequences are then ... conclusion, we are now at the stage where we have the tools that enable characterization of the full extent of inversions in the human genome and their contribution to human variation and disease Abbreviations...

Ngày tải lên: 11/08/2014, 12:20

... RefSeq were split at the 5' end of the RefSeq sequence refereed research Protein sequence mapping Synthesis and analysis deposited research Human and mouse UniGene and RefSeq, MGI, and HGI sequences ... the mouse and human genomes, aligned using the AVID program [35] and then fed into the rVista program [36] From this, we identified the conserved regions in this gene between mouse and human, including ... i and probe j in the current transcriptional model, µ is the grand mean term, φi is the probe-specific term for probe j in the model, θi is the sample-specific term for sample i, and εj is the...

Ngày tải lên: 14/08/2014, 14:21

... and the transcripts targeted by miRNA The first part uses the Ensembl database and the PERL API [108] to map the HUGO identifiers to Ensembl identifiers (the gene, the transcript and the protein) ... target analysis and drafted the major part of the manuscript AEL carried out the microarray analysis, ubiquitin predictions and function analysis and assisted in all other aspects of the work ... levels and protein levels for a high proportion of these disordered proteins compared to the ordered proteins On the other hand, it is shown that for a significant proportion of highly disordered proteins,...

Ngày tải lên: 14/08/2014, 21:20

... further strengthen the use of zebrafish Recently, there is increased generation and analysis of zebrafish models of human diseases (Amatruda and Patton, 2008; Liu and Leach, 2011) Owing to the ease ... genetic and biological similarities to human (Fausto and Campbell, 2010) Furthermore, the mouse together with other mammalian models including rats has made the prediction of drug efficacy and toxicity ... generally mimics human diseases and offers the ability to quickly and inexpensively test the efficacy and safety of compound libraries (Amatruda and Patton, 2008; Liu and Leach, 2011; Zon and Peterson,...

Ngày tải lên: 10/09/2015, 15:48

... determined by dividing the total energy by the units of time in the interval integrated demand: The average of the instantaneous demands over the demand interval demand interval: The time period during ... within a system and the metered line flows into the system, less the metered line flows out of the system coincident demand: The sum of two or more demands that occur in the same demand interval ... for public comment on the results of the study; and (3) submit to the President and Congress a report describing-(A) the results of the study; and (B) information relating to the public comments...

Ngày tải lên: 22/09/2016, 20:28

... the oligomerization of Vpu and the domains for this, we have used genetic, biochemical and biophysical methods, and two divergent Vpu proteins, one from a subtype C primary isolate and the other ... recruits other proteins such as Skp1, Cul-1 and the Cdc34 E2 ubiquitin ligase [11] This results in ubiquitination of CD4, its dislocation from the ER and degradation by the proteosome [12,13] The stable ... interactions Further, the NL Vpu and R5 Vpu proteins interacted with each other All these interactions were found to depend upon the TM domains, but not on the cytoplasmic domains of Vpu The trans- formants...

Ngày tải lên: 18/06/2014, 18:20

... manuscript DF, MS and FW were involved in data analysis and interpretation, and writing of the manuscript WK was involved in study design and data analysis BE was involved in study design and data collection ... two hours after the onset of the event without further complications The treating physician classified the severity as moderate and causative relation to PC treatment as unknown The second patient ... data management, statistical analysis and data interpretation RS was involved in study design, data analysis, and writing of the manuscript All authors read and approved the final manuscript Competing...

Ngày tải lên: 13/08/2014, 21:21

... (DSLS) thermal-melt assay PRPP, IMP and GMP were the top hits and therefore were identified as potential substrates, products or regulatory ligands for PRTFDC1 The catalytic efficiency and substrate ... II The hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The ... Environment, Agricultural Sciences and Spatial Planning (to L.W and S.E.), the 4928 Swedish Research Council (to H.E and P.N.) and the Swedish Cancer Foundation (to H.E and P.N.) The Structural Genomics...

Ngày tải lên: 15/02/2014, 01:20

... expressed over the detection sensitivity in the western blot analysis and well transferred in the membrane fraction Binding properties of muscarinic ligands with M2::GOA-1 fusion protein The binding ... for carbamylcholine) [25] These results indicate that M2 in the GOA-1 fusion protein is functional, and the ligand-binding properties agree with that of the Gai1 fusion protein Activation of GOA-1 ... (aN–a1), the aF–aG region and the C-terminal region (a4–a5) were well conserved between GOA-1 and Gao [31,32] The five regions of the Ga-subunit involved in receptor recognition are the a2 helix, the...

Ngày tải lên: 07/03/2014, 11:20

... (Fig 5B) These results further indicate the involvement of both MAPKs, ERK1 ⁄ and p38, in the signaling pathways, consistent with the inhibition of the antiproliferative activity of ATP by the MEK1 ... mediating the observed response and the functionality of the proteins in the signaling mechanisms that lead to the attenuation of cell cycle progression Experimental procedures Cell culture and reagents ... family comprises the ionotropic P2X receptor ion channels and the metabotropic, G protein- coupled P2Y receptors [1–3] Seven subtypes of the P2X receptors (P2X1)7) and eight subtypes of the P2Y receptors...

Ngày tải lên: 07/03/2014, 12:20

... many reports suggest that these proteins stabilize the XPC protein by protecting it from 26S proteasomedependent protein degradation [5–7] Another biochemical analysis of the damage-recognition ... to be the cornerstones for the boundaries of the helices, and their periodical presence made the XPCB domain fold in a compact manner by introducing helical breaks and turns or kinks (Figs and ... correlation times (F), the exchange rates (G), and the model types (H) from the TENSOR2 analysis The values marked by asterisks (*) in (C) and (G) are 24.0 ± 0.94 (s)1) and 14.8 ± 1.62 (s)1),...

Ngày tải lên: 07/03/2014, 17:20

... and molecular mechanism Nature 349, 117–127 Herrmann C, Martin GA and Wittinghofer A (1995) Quantitative analysis of the complex between p21ras and the Ras-binding domain of the human Raf-1 protein ... against the protein or mant-GTP concentration Using a linear fit, the association rate constants were extracted from the slope (kon), and the intercept represents the dissociation rate (koff) In the ... coefficients of 2.4 and 1.9 and dissociation constants (KdHill) of and lm for hGBP5a ⁄ b and hGBP5ta, respectively The proteins are very similar in terms of their enzymatic activity, so the activity...

Ngày tải lên: 15/03/2014, 10:20

... excluded the residues 45–58 in the former model and 40, 49 and 55–58 in the latter model of SUMO-2 and the equivalents of ubiquitin Although the sequences have only 18% identity, the protein folds ... crystal structure and SUMO-1 NMR structure is particularly evident in the regions of 28–43 and 71–83, that correspond to the strand b2, the N terminus of the helix a1, the helix a2, and the connecting ... geometry and crystal contacts were analysed using the programs PROCHECK and AREAIMOL of the CCP4 package [21] Results and Discussion Structure determination and refinement Analysis of the diffraction...

Ngày tải lên: 16/03/2014, 18:20