the hallmark of hepatocellular carcinoma

... sites in the OPN promoter Although the rests of transcription factors were upregulated in gene-chip analysis, they lacked the reported binding site in the OPN promoter and may act by the way of combining ... seeded the day before transfection Then, ug of plasmid DNA and ul of LipofectAMINE 2000 (Invitrogen, Carlsbad, CA), diluted with Opti-MEM, were mixed gently and incubated with cells Together, the ... 37°C with 5% CO2 After 40 hours, the cells removed from the upper surface of the Matrigel by scrubbing with a cotton swab and cells that migrated to the underside of the membrane were stained with...

Ngày tải lên: 10/08/2014, 10:20

... is a safe therapeutic choice, the concern of the choice of hepatectomy in this group of patients is a higher risk of recurrence than transplantation The aims of this study were to find the recurrence ... Despite the lack of a high-grade evidence base for either resection or transplantation, the result of these treatments provides 5-year survival rates of up to 70% in selected patients These are ... (16.4%), the disease free interval ranged from 60.76 to 181.78 months Outcome of hepatectomy within UCSF criteria To determine the outcome of hepatectomy for hepatocellular carcinoma, the survival...

Ngày tải lên: 09/08/2014, 01:24

... liver tissue of healthy dogs, measured by quantitative Real-Time PCR Data represent mean ± SE of the groups The "n" for these figures stands for the fold change of the gene expressions of the cell ... definitive proof of the hepatocytic and neoplastic nature of the cHCC cells Positive staining for hepatocyte marker HepPar1 strongly indicates the hepatocytic origin of the cultured cells [10] The neoplastic ... [10] The neoplastic nature of the cells can be deduced from the pleiomorphism of the cells, the number of sometimes bizarre mitotic figures The simultaneous presence of CK7 and HepPar1 positive...

Ngày tải lên: 13/08/2014, 13:20

... determination by the QuantiGene 2.0 Reagent system The RNAs of the different PBMC samples from all of the bleeding times were used in duplicate to determine the levels of expression of the rabbit IFN-γ ... responses in humans Further improvements of the recombinants, using the E6 and E7 transgenes deleted of the p53 and p105Rb cellular binding domain, might further increase the safety of the vaccine Recently, ... evidence the degree of variability among the animals and the trend shown by each of them overtime Also, to better compare the E7 humoral response during prime and boost immunisations when the immunogen...

Ngày tải lên: 18/06/2014, 16:20

... alterations of tumor cells and contributes to the growth of prostate carcinoma and is a predictor of aggressive features of this cancer [14,15] Up to date, the clinicopathologic/prognostic implication of ... high-risk group as the presence of the three factors (including p300 expression, AFP level and vascular invasion), an intermediate-risk group as the presence of two factor (regardless of their identity), ... accompanied by loss of the other allele, was observed in certain types of tumors, including colorectal, gastric and breast cancers [8,9] In addition, the frequency of promoter methylation of p300 gene...

Ngày tải lên: 18/06/2014, 16:20

... thickness, the cirrhotic tissue around the nodule would work as a thermal insulation, avoiding the dispersion of the heat generated around the RFA needle electrode In the current study we found that the ... when feasible, either PEI or TACE was performed An ablation zone beyond the borders of the tumor was defined as ablation margin 329 not be further treated because of the onset of multicentric ... multicentric hepatocellular carcinoma, either TACE or only symptomatic relief care was performed when required The primary effectiveness rate was assessed on every single nodule on the basis of the absence...

Ngày tải lên: 08/08/2014, 17:20

... gross invasion of the right or left main branches of the portal vein or the hepatic veins [12] Microvascular invasion indicated the presence of clusters of cancer cells floating in the vascular ... considered for these tumors given the lack of effective alternative therapies Further studies are needed for understanding of molecular biology of BTT may yield therapeutic tools that can improve the prognosis ... in the design and coordination of the study, carried out the critical appraisal of studies and wrote the manuscript CS, ZL, and YZ developed the literature search, carried out the extraction of...

Ngày tải lên: 09/08/2014, 01:24

... presence of metastasis, death and cause of death VNPI scores were calculated using both the original and modified criteria The length of the follow-up period was calculated from the date of the first ... all of their patients with DCIS whilst the majority of centres based their decision to give or withhold RT on the presence or absence of certain tumour characteristics thought to influence the ... these groups that the benefit: risk ratio is likely to be highest The effect of including the small number of patients with tumours that did not have their margin width recorded (n = 18) in the...

Ngày tải lên: 09/08/2014, 07:21

... cancer, and the lung is the most common site of extracranial metastases On the other hand, the second route is the osseous route via Batson's venous plexus to the skull In this group, bone is the most ... table of the skull [7] Therefore, it is difficult to find the skull metastasis from HCC without the presence of other bone metastases This patient initially visited the hospital due to the incidentally ... pathways in the advanced stage of HCC [6,7] One of them is the hematogenous route via the lung to the brain parenchyma without skull involvement In this group, the character of HCC is defined as a "neutrophilic"...

Ngày tải lên: 09/08/2014, 07:21

... view of the lateral beam of the radiation therapy This shows the metastatic lung tumor is outside the target area anti-CTLA-4 antibody [15] Although these hypotheses have some merit, at present they ... increases the activity of natural killer cells [13,14] Dewan et al presented the hypothesis that the type of dose fractionation regimen determines the ability of radiotherapy to synergize with ... performed after the radiation therapy, which showed a remarkable reduction in the mediastinal lymph node Additionally, shrinking of the tumor in his right lower lobe, outside of the radiation field,...

Ngày tải lên: 11/08/2014, 00:23

... remained out of favor for the diagnosis of HCC because of the lack of differentiation between HCC glucose metabolism and normal liver tissue However, on the basis of a literature search, the PET scan ... targeted therapy immediately after the PET scan identified the metastatic lesion over the chest wall The response to sorafenib treatment provides further progression-free survival Therefore, the early ... informed consent was obtained from the patient for the publication of this case report A copy of the written consent is available for the review by the EditorChief of this journal Abbreviations AFP:...

Ngày tải lên: 11/08/2014, 00:23

... significance between the other groups The scatter diagrams of the studied cytokines in the different study groups are shown in Figures 2, 3, and Correlation was done between the serum levels of the studied ... variety of mechanisms, one of which is postulated to be the production of soluble forms of Fas (sFas) that normally binds to Fas-L, thus blocking the signaling of the membrane-bound form of Fas ... induces the surface expression of IL-2 receptor (IL-2R) and, consequently, the production of its soluble form, sIL-2R The excess of sIL-2R is capable of binding IL-2 and causes the inhibition of an...

Ngày tải lên: 13/08/2014, 13:20

... added to each well of PLOS ONE | www.plosone.org Ethics statement The study protocol was approved by the Internal Review Board of the Joint Ethics Committee of The University of Hong Kong January ... proliferation Many of the HCC patients are often, and unfortunately, being excluded from the surgical resection or chemotherapy because of their poor underlying liver functions Thus it is worth of investigating ... target of rapamycin up-regulation of pyruvate kinase isoenzyme type M2 is critical for aerobic glycolysis and tumor growth Proceedings of the National Academy of Sciences of the United States of...

Ngày tải lên: 23/09/2015, 14:47

... pAR[DRI] vector Of the 750 000 plaques analyzed, two of them hybridized with the probe The sequences of the inserted cDNA were identical In frame with the kgt11 Lac Z coding sequence they contained ... where they might interact Indeed there was no evidence that the RIIa regulatory subunit of PKA is expressed anywhere other than the cytosol and the cytoplasmic surfaces of membranes [29] On the other ... protein of the endoplasmic reticulum [22], calreticulin, a protein of the endoplasmic reticulum lumen [23], HIP/PAP, the RIIa and the Ca subunits of PKA were checked by immunoblotting in both the...

Ngày tải lên: 23/03/2014, 13:20

... as the period from the date of hepatectomy to the date of recurrence as detected by imaging studies Overall survival (OS) was defined as the period from the date of hepatectomy to the date of ... affect the long-term outcomes A staging system should be capable of accounting for these factors and the most important drawback of the TNM-7 staging system is the lack of incorporation of host ... Background: The aim of this study was to assess the validity of the 7th edition of the American Joint Committee on Cancer (AJCC) TNM system (TNM-7) for patients undergoing hepatectomy for hepatocellular...

Ngày tải lên: 09/08/2014, 02:21

... limited by the shortage of donors [21] Hepatic resection remains the treatment of choice offering the possibility of cure, but the long-term prognosis remains unsatisfactory due to the high recurrence ... recurrence is considered one of the most important factors that impact the prognosis of HCC patients [25] The present study attempts to determine the impact of the type of liver resection (anatomical ... throughout the follow-up period End Points The main end points of the study were: 1- In-hospital mortality, morbidity and length of hospital stay 2- The detection of early disease recurrence through the...

Ngày tải lên: 09/08/2014, 03:21

... Asia than the rest of the world Of the various types of liver cancers, hepatocellular carcinoma (HCC) is the most common The development of HCC is a multi-step process During this process, the aberrant ... treatment of cancer, we need to better understand the functions of the HDAC isoforms and their mechanism of actions Numerous studies tried to test the effect of knocking down each individual isoform of ... also elicited their mechanism of actions by demonstrating the importance of their enzymatic activity as well as the compensatory effects on each other Understanding these members of the HDAC family...

Ngày tải lên: 10/09/2015, 08:27

... structures of the founding member and/or the best characterized example of the main LIM-protein families are shown The number of known members of each family is indicated in parentheses The colored ... Figure of Chapter 2.1 Map of the pDONRTM221 Vector……………………………………………55 2.2 Map of the pcDNA-DEST40 Vector……………………………………… 56 2.3 Map of the pcDNA-DEST47 Vector……………………………………… 57 2.4 Map of the pCR®-Blunt ... insights into the roles of two interesting genes which are involved in tumor metastasis and growth The data also strengthens the understanding of the effect of p53 on cellular processes in the molecular...

Ngày tải lên: 13/09/2015, 19:46

... human hepatocellular carcinoma Gankyrin binds to the cell-cycle regulator CDK4 and the S6b ATPase subunit of the regulatory component of the proteasome Based on the conserved gene expression profile ... in human hepatocellular carcinoma cells In addition, this represents a novel approach for the identification of potential targets in the diagnosis and treatment of human hepatocellular carcinoma ... decision is made either for the null or the alternative hypothesis In order to keep the number of false positive decisions small, the two testing procedures were set up to control for the family-wise...

Ngày tải lên: 31/10/2012, 15:28

... 4A) Neither blocking of miR-373 with ASOs nor overexpression of miR-373 had any effect on the intensity of EGFP fluorescence from the vector containing mutations in the region encoding the miR-373 ... [19] The function of miR-373 on HCC cells needs to be elucidated in the future The fundamental function of miRNAs is to regulate their targets by direct cleavage of mRNA or by inhibition of translation ... mutant), the 3¢-UTR and mutant 3¢-UTR fragments of PPP6C transcripts amplified by RT-PCR were inserted into the vector backbone downstream of the EGFP gene between the BamHI and EcoRI sites of the...

Ngày tải lên: 14/03/2014, 23:20