the function of protocols

Ngày tải lên: 01/04/2014, 12:20

Ngày tải lên: 03/01/2014, 19:44

... [11,12]. The study of these events is taxing, as the biosynthesis of glycoproteins is not tem- plate driven. This results in the formation of so-called ‘glycoforms’ [11,12], proteins with the same ... 1957 catalyze enhanced hydrolytic degradation of the target protein. More recently, the glycoMTS method has allowed the synthesis of the first examples of a homogeneous protein bearing symmetrically ... scaf- fold. These terms reflect not only the linearity or convergence of the chemical steps that may lead to a given synthetic protein, but also the structural strategy that links the (linear) segments of...

Ngày tải lên: 18/02/2014, 17:20

... amount of RXR pulled down by EcR in the presence of ligand was maximum in the case of HsRXR and minimum in the case of the Hs–LmRXR chimera, and LmRXR was between these two (Fig. 9). These data ... concentrations of ligands for the transactivation of genes and functions well in vivo. The RXR chimeras containing most of HsRXR and helix 9 and the loops on either side of helix 9 from HsRXR or mutants of ... been used in the place of USP in all EcR gene switches developed to date. One of the major limitations of cur- rent versions of EcR gene switches is the requirement of micromolar concentrations of ligand...

Ngày tải lên: 07/03/2014, 12:20

Ngày tải lên: 08/03/2014, 18:20

Ngày tải lên: 14/03/2014, 23:20

Ngày tải lên: 16/03/2014, 16:20

Ngày tải lên: 24/03/2014, 03:21

Ngày tải lên: 28/06/2014, 15:20

... posi- tioned in the connecting loops. Approximately one- third of all mutations in the BTK gene are missense and some of these reduce the stability of the protein. This is exemplified by mutations in the ... identify individuals with mild disease, thereby providing the missing data. The phenotype of XLA and the potential of BTK and ITK inhibitors The outcome of defective BTK signaling in humans has ... regarding their potential function, it was the identification of BTK, as the kinase affected in XLA [9,10], which immediately made TFKs known to the wider scientific community. In the same year, the xid...

Ngày tải lên: 14/02/2014, 18:20

... [9]. The KIND domain in these proteins is localized to the N-terminal region, and their specific functional domains are located in the C-termi- nal region. The C-lobe of protein kinases mediates the interaction ... under- stood, the data obtained in the present study indicate that the 43 residues (amino acids 702–744 in mice) that reside in the middle region of the CD act as the v-KIND binding core. The v-KIND-binding ... between the Leu residues from v-KIND and MAP2 contributes to the interaction between the two proteins. In conclusion, the present study has clarified the structural and functional importance of the...

Ngày tải lên: 14/02/2014, 19:20

... in all of these conditions [22]. In the trau- matically injured brain, however, the synthesis of MT1 and MT2, as well as the synthesis of GIF, are up-regu- lated by reactive astrocytes in the vicinity ... loss in the AD brain. Further investigation of the increased neurotrophic activity of the AD brain revealed that it correlated with the loss of a specific neuroinhibitory factor, rather than the presence ... inhibit neurite outgrowth (as described above). The functional role of GIF synthesis and secretion in the brain Taken together, the regulation of GIF synthesis by reactive astrocytes, and its subsequent...

Ngày tải lên: 16/02/2014, 15:20

... W,is simply the sum ofthe body mass of all individuals. For organisms ofsimilar size, it can be estimated by taking the product of the population, N,andthebodymass, M. Similarly, the store of each ... obvious. We asked the author(s) of each paper to examine the importance and role of body size in the systems in which they work. Essentially the book builds from the level of the individual and ... links between the processing of energy and elements at the individual level to the flux, storage and turnover of these elements at the level of ecosystems. Our second example concerns the role of metabolism...

Ngày tải lên: 17/02/2014, 19:20

... dif- ferent apoptotic stimuli. Thus, the apoptotic volume decrease may further disarrange the balance between survival and death signals, thereby promoting execu- tion of the apoptotic program. Concluding ... changes trigger signals involved in the regulation of metabolism, gene expression and the susceptibility to different kinds of stress [6]. For example, the inhibi- tion of autophagic proteolysis by ... a functional outcome. This minireview summarizes recent progress in the understanding of how osmosensing and osmosignaling integrate into the overall context of growth factor signaling and the...

Ngày tải lên: 18/02/2014, 16:20

... interruption of the geometry of the recognition helix for either Antp or the TTF-1 HD, the anomalous amide exchange pattern and the NOE connectivity data of the C-terminal portion of helix III ... respec- tively. The proximity to one of the intercepts between the theoretical and fitting curves reflects the relative contribution of each component Lorentzian function to the specific spectral density of ... conflict with the global interpretation of the data. With the exclusion of the N-terminal octapeptidyl and C-terminal nonapept- idyl fragments of Glu17, Leu34 and Lys46, the average of the { 1 H}– 15 N...

Ngày tải lên: 18/02/2014, 16:20

... greater than the rate of decomposition. Therefore, the nature of the accumulated organic matter can then be used as a proxy to represent the composition of the former wetland communities at the site. ... Stratigraphic Commission, the beginning of the Pleistocene now coincides with the base of the Quaternary ( Gibbard et al. 2009 ) . The Holocene begins at the end of the Pleistocene (Walker et ... environmental information at the regional, rather than at the local scale. Nevertheless, the spatial resolution of the pollen fl ora is strongly in fl uenced by the size and nature of the depositional setting...

Ngày tải lên: 18/02/2014, 20:20

... in regulation of Nox3, the role of the PX domain in Nox3 activity remains unknown. In the process of cloning of human Noxo1, some spliced transcripts of the NOXO1 gene have been identified [15,17–19]. They ... between the pair of primers (Fig. 2A). The lengths of the PCR products of Noxo1b and Noxo1c were 225 and 240 bp, respectively. To delineate the difference in the two products, we subjec- ted the ... activity of the PX domains of Noxo1b and Noxo1c The membrane localization of Noxo1b is mediated in part by binding of the PX domain to membrane phos- pholipids [19]. Less association of Noxo1c with the membrane...

Ngày tải lên: 19/02/2014, 06:20

... studies. The high selectivity of the a-conotoxins, together with the possibility of obtaining detailed information on their three dimen- sional structures and the relative ease of synthesis, makes them ... identified in a cloning approach making use of the high conservation of the 3¢ untranslated region and the intron preceding the sequence of the a-prepropeptide [39]. The peptide was characterized on oocyte-expressed ... and the deter- mination of their precise subunit composition and physiological function( s). Compared to the muscle nAChR, relatively little is known about the function and composition of the...

Ngày tải lên: 19/02/2014, 12:20

... attributed to the lack of a hydrophobic core typically found in globular proteins. Instead, there is a rung of disulphides down the middle of the protein. The addition of dithiothreitol destroys the TH activity ... activity. Examination of the crystal structure of sbwAFP did not Fig. 6. Bound water molecules extend the ice-binding face of insect AFPs. The position of the water oxygen atoms along the TXT f ace found in any of ... determine the handedness of the proteins, or may prevent the unfolding of the protein at cold temper- atures. The b-helix as an AFP structural motif? The sbwAFP and TmAFP structures represent the first AFPs...

Ngày tải lên: 19/02/2014, 16:20

... to change the size of their asset portfolio because of the size of their markets and/or because of regulatory restrictions on their expansion. In this event, the efficiency of smaller firms ... data set. IV. The Profit Efficiency Effects of Megamer~ers The Level of Profit Efficiency. We begin discussing the results with some information on the levels of profit efficiency of merging and ... ‘ standard profit efficiency model in equations (1) and parameters of the model. The level of profit efficiency the ratio of predicted profits to maximum or optimal profits on the frontier...

Ngày tải lên: 06/03/2014, 08:21