the erk1 2 map kinase signaling pathway in tumor progression and metastasis

... remodeling of the ECM underlying tumor and endothelial cells, thereby facilitating cell invasion [23 25 ] Progress in the field and the generation of genetic tools (i.e heparanase transgenic and knockout ... been established clinically [23 ,25 ,31] and in several in vitro and in vivo model systems, including wound healing [29 ,57], tumor xenografts [ 52, 55], Matrigel plug assay [57] and tube-like structure ... expression and function in tumor progression and metastasis Nat Med 5, 793–8 02 23 Ilan N, Elkin M & Vlodavsky I (20 06) Regulation, function and clinical significance of heparanase in cancer metastasis and...

Ngày tải lên: 15/03/2014, 23:20

... N-terminal and ankyrin (ANK) repeats immediately C-terminal of the Arf GAP domain Other prominent features of ASAPs include the Bin, amphiphysin and Rvs 161 and 167 (BAR) domain at the N-terminus and ... which bind to the substratum on the extracellular face of the plasma membrane and associate with proteins such as focal adhesion kinase (FAK), vinculin, paxillin, integrin-linked kinase and talin ... (43.9) 64 (56.6) 0.054 1 02 125 59 (57.8) 54 (43 .2) 43 ( 42. 2) 71 (56.8) 0. 028 26 . 42 ± 28 .1 26 .69 ± 27 .9 26 .15 ± 28 .41 0.886 37.38 ± 28 .55 37.8 ± 29 .31 36.97 ± 27 .89 0. 827 TABLE Clinicopathological correlation...

Ngày tải lên: 01/10/2015, 17:28

... N-terminal kinase by MEKK -2 and mitogen-activated protein kinase kinase kinases in rheumatoid arthritis J Immunol 20 04, 1 72: 16 12- 1618 Lu H, Sun T, Yao L, Zhang Y: Role of protein tyrosine kinase in ... inhibitors used in this study, including the Erk activation inhibitor, PD98059 [13], the c-Jun N-terminal kinase (JNK) inhibitor, SP600 125 [14], p38 inhibitor, SB2 021 90 [15], and the NFκB inhibitor, ... that MAPK pathways might be involved in IL-1β-induced SYN expression To further elucidate which MAPK pathway is involved in regulating IL-1β-induced SYN expression, specific inhibitors of MAP kinases...

Ngày tải lên: 09/08/2014, 08:23

... YHUM214 YHUM216 MR161 MR211 MR298 AR2 MR287 AR3 AR4 Source CEN.PK2; MATa ras2::kanMX ura3– 52 leu2–3, 1 12 his3-D1 trp1 28 9 MAL2–8c SUC2 CEN.PK2; MATa gpa2::TRP1 ras2::kanMX ura3– 52 leu2–3, 1 12 his3-D1 ... trp1 28 9 MAL2–8c SUC2 [p 426 MET25-RAS2] CEN.PK2; MATa ras1::S.pombe his5+ ras2::kanMX ura3– 52 leu2–3, 1 12 his3-D1 trp1 28 9 MAL2–8c SUC2 [pFL38-RAS2] S 127 8b; MATa ura3– 52 his3:hisG leu2::hisG sut2:: ... Plasmid inserts are indicated on the left margin e.p., empty plasmid (YEp351) Note that the parental strains CEN.PK2 Dgpa2 Dras2 and CEN.PK2 Dras1 Dras2 contained plasmid p 426 MET25-RAS2 (2l plasmid)...

Ngày tải lên: 07/03/2014, 15:20

... for 15 in 0.1% Triton X-100 in PBS, rinsed in PBS, fixed for in -20 °C acetone (analysis grade), and the sections were rehydrated in PBS containing 0.5% BSA (BSA-PBS) Then the sections were incubated ... TGF-beta2 in human retinal pigment epithelial cells [J] Exp Eye Res 20 07, 84(5):8 12- 822 Akiyama T, Ishida J, Nakagawa S, et al: Genistein, a specific inhibitor of tyrosine-specific protein kinases ... of cells in the S phase and expression of protein Cyclin A via the TK signaling pathway Taking together, PAP promotes chondrocyte proliferation by activating TK signaling pathway Acknowledgements...

Ngày tải lên: 20/06/2014, 00:20

... cell signaling pathways regulate survival and activation in the periphery and in the thymus, the immune system must balance antigen presentation and proinflammatory outcomes in the periphery in ... cells The two main NFκB activation pathways are marked in blue IL, interleukin; IRAK, IL-1 receptorassociated kinase; MAPK, mitogen-activated protein kinase; mTEC, medullary thymic epithelial cell; ... deletion in the NOD mouse; a polygenic trait characterized by defective induction of Bim Immunity 20 04, 21 :817-830 Baldwin AS Jr: The NF-kappa B and I kappa B proteins: new discoveries and insights...

Ngày tải lên: 09/08/2014, 06:23

... kinase (MAPKAPK2 or MK2) [ 126 ] MAP kinase- activated protein kinase (MK3) [ 127 ] MAP kinase interaction protein kinase (MNK1) [ 128 ] p38 regulated/activated kinase (PRAK) [ 129 ] Mitogen- and stress-activated ... for all the members of the MAPK family and is typically composed of a highly conserved MAPK module comprising three kinases, namely MAPK kinase kinase (MKKK), MAPK kinase (MKK), and MAPK [20 ] (Fig ... MSK2 and MSK1 mediate the mitogen- and stress-induced phosphorylation of histone H3 and HMG-14 EMBO J 20 03, 22 :27 88 -27 97 43 Saklatvala J: The p38 MAP kinase pathway as a therapeutic target in inflammatory...

Ngày tải lên: 09/08/2014, 07:20

... Fukuda R, Kinoshita Y: Activation of prostaglandin E2-receptor EP2 and EP4 pathways induced growth inhibition in human gastric carcinoma cell lines J Lab Clin Med 20 02, 140: 92- 1 02 Dubinett SM, ... in NSCLC specimens, including all squamous cell lung cancer and 70% of adenocarcinomas [25 ] However, the involvement of COX -2 in the angiogenic response of tumor cells and the role of COX -2 in ... substituting PBS for primary antibody For this study, the intensity of VEGF and COX -2 staining were scored on a scale of 0-3: 0, negative; 1, light staining; 2, moderate staining; and 3, intense staining...

Ngày tải lên: 10/08/2014, 10:20

... with the increase of luciferase activity in the liver Effect of the MAP kinase inhibitors on IκBα induction by LPS We examined the effect of MAP kinase inhibitors SB203580, PD098059 and SP600 125 ... bortezomib and several MAP kinase inhibitors on LPS-induced IκBα expression The results that follow suggest that, in addition to NF-κB, the MAP kinase signaling pathway is involved in controlling IκBα ... LPS We demonstrated involvement of both the NF-κB and the p38 MAP kinase signaling pathways in the induction of IκBα expression by LPS Clinically, NF-κB activation is involved in many chronic disease...

Ngày tải lên: 11/08/2014, 08:21

... al., 20 01) 1 .2 Tyrosine kinase signaling cascade 1 .2. 1 Protein tyrosine kinases (PTKs) Protein tyrosine kinases are enzymes that carry out tyrosine phosphorylation by catalyzing the transfer of the ... hypersecretion in the airways is still uncertain The finding that activation of NF-κB via a c-Src-Ras-MEK1 /2- MAPK-pp90rsk signaling pathway binding to a κB site in the 5’-flanking region of the MUC2 gene and ... IgE inducing expression of the FcεRII and MHC class II enhancing the expression of integrins, including CD11b, CD11c, CD18, and CD29 inducing MHC class II and CD23 expression Inhibiting the production...

Ngày tải lên: 15/09/2015, 22:09

... (PTKs) 25 25 iii 1 .2. 1.1 Role of PTK signaling cascade in allergic inflammation 1 .2. 2 Mitogen-activated protein kinase (MAPK) cascades 28 29 1 .2. 2.1 MAPK 29 1 .2. 2 .2 Role of MAPK cascade in allergic ... 1.1 .2 Therapeutic targets of asthma 20 1.1 .2. 1 Current therapy for asthma 21 1.1 .2. 2 Novel therapeutic targets for asthma 22 1 .2 Tyrosine kinase signaling cascade 1 .2. 1 Protein tyrosine kinases ... allergic inflammation 31 1 .2. 3 Phosphoinositide kinase (PI3K) 33 1 .2. 3.1 PI3K cascade 33 1 .2. 3 .2 Role of PI3K cascade in allergic inflammation 37 1.3 Inhibitors of the tyrosine kinase signaling cascade...

Ngày tải lên: 16/09/2015, 17:11

... 28 33.535 26 21.471 25 56 .21 5 22 83 .21 8 21 09.054 21 66.9 32 221 2.978 C3 28 49 .24 1 26 21.197 25 55.979 23 08.088 22 12. 749 21 08.8 32 2151.991 1970.785 28 33.334 25 75.086 26 21 .27 5 23 86.9 62 221 2.806 * 23 08.159 ... consisting mainly of the FEBS Journal 27 5 (20 08) 4479–4489 ª 20 08 The Authors Journal compilation ª 20 08 FEBS 4481 28 33.537 26 21.455 25 56 .22 4 22 13.147 22 83 .22 0 21 09.0 72 21 52. 230 1917.0 72 2833.535 26 21.471 ... 1661.757 1713. 724 1605.744 1494.7 52 1419.657 125 3.606 0.5 11 72. 646 774 .29 2 1.0 1014.535 1065.993 1.5 959.450 8 42. 465 2. 0 895.4 02 x10 C6 28 49.451 25 91.1 42 2 623 .34 * 22 12. 893 21 08.941 21 52. 088 1970.869...

Ngày tải lên: 07/03/2014, 06:20

... This group of protein kinases includes the ribosomal S6 kinase (RSK) (26 ), the MAPK/SAPK-activated kinase (MSK) (27 ), and MAPK signal-interacting kinase (MNK1) (28 ,29 ), although the two latter ones ... mitogen-activated protein kinase and S6 kinase sig- 22 60 61 62 63 64 65 66 67 68 69 70 71 72 Rubinfeld and Seger naling pathways by 12- O-tetradecanoylphorbol-13-acetate (TPA) and insulin Evidence for involvement ... kinase (MAPK) subfamilies include the extracellular signal-regulated kinases (ERK1/ 2) , the c-Jun N-terminal kinases (JNKs), and the p38 kinases These kinases are differentially activated in response...

Ngày tải lên: 11/04/2014, 09:50

... expressing HIF-1α and HIF -2 proteins in normal, rheumatoid, and osteoarthritic synovium Normal (n = 22 ) OA (n = 34) RA (n = 22 ) HIF-1α HIF -2 HIF-1α HIF -2 HIF-1α HIF -2 Minimum 0.00 0.00 20 .00 ... 40–63%) in RA and 50% (range 0–100%; 95% CI 43–66%) in OA (Table 2) Normal synovia were, in most cases, unreactive, and only in a small percentage of cases (6 /22 and 4 /22 for HIF-1α and HIF -2 , respectively) ... HIF-αs in the pathogenesis of RA and OA In the present study, we investigated the immunohistochemical expression of HIF-1α and HIF -2 in synovial tissues in RA and OA The results were related to the...

Ngày tải lên: 09/08/2014, 01:21

... 10: 120 4- 121 1 Wilks AF: The JAK kinases: not just another kinase drug discovery target Semin Cell Dev Biol 20 08, 19:319- 328 Demoulin JB, Renauld JC: Signalling by cytokines interacting with the interleukin -2 ... RA [29 ] CP690,550 could be useful in blocking the JAK/STAT-mediated proinflammatory responses including IL-6-mediated signaling in RA The MAPKs play an important role in the induction of pro-inflammatory ... with inhibitors specific for each of the MAPKs, ERK1/ 2, p38 and JNK1 /2, and then stimulated with OSM As shown in Figure 6A, inhibition of JNK or ERK1/ 2 signaling led to partial reduction of the...

Ngày tải lên: 12/08/2014, 15:23

... Cyclic-di-GMP signaling 12 2 .2. 2 Occurrence of c-di-GMP signaling enzymes 18 2. 2.3 Redundancy of c-di-GMP signaling enzymes 20 2. 2.4 GGDEF-EAL bidomain proteins 23 2. 3 Bacterial motility 24 2. 3.1.Flagellar-mediated ... reported 11 ,24 8 proteins that contain GGDEF and EAL domains Of these, 9943 proteins possess the GGDEF domain and 5574 contains the EAL domain (Seshasayee et al., 20 10) 3769 proteins in this list ... distinctive signaling systems (Aravind et al., 20 10) The mainstream view in the early 1990s was that eukaryotic and prokaryotic signaling systems are different from each other In areas where there...

Ngày tải lên: 09/09/2015, 17:53

... is the fraction of proliferating cells in the tumor of control mice and k d is the fraction of proliferating cells in the tumor of PMN-MDSC-depleted mice For tumors with ellipsoid shape, the ... expression in the lungs of control vs PMNMDSC-depleted mice, k c is the fraction of proliferating cells in the lungs of control mice and k d is the fraction of proliferating cells in the lungs ... is the number of melanoma cells at the end of the experiment, N is the number of melanoma cells at the onset of the experiment, k is the fraction of proliferating cells, t is the duration of the...

Ngày tải lên: 10/09/2015, 15:50

... of GA signaling 17 2. 2 .2. 1 Soluble GA receptor GID1 17 2. 2 .2. 2 F-box proteins essential for GA signaling pathway 18 2. 2 .2. 3 GTP-binding proteins 18 2. 2 .2. 4 PICKLE (PKL) 19 2. 2 .2. 5 PHOTOPERIOD RESPONSIVE ... 19 2. 2 .2. 6 MYB transcription factors 20 2. 2.3 Negative regulators of GA signaling 21 2. 2.3.1 DELLA genes 2. 2.3.1.1 Two categories of mutations of GAI gene 21 21 2. 2.3.1 .2 GAI and RGA together ... genes 24 2. 2.3 .2 SPINDLY (SPY) 24 2. 2.3.3 SHORT INTERNODES (SHI) 25 2. 2.4 Inhibitor of an inhibitor: GA promote plant development by targeting DELLA to degradation via the ubiquitin-proteasome pathway...

Ngày tải lên: 13/09/2015, 20:31

... CELL LINES 62 2.5.1 Mutational analysis of p53 62 2.5 .2 Gene amplification of hdm2 63 2. 5.3 Mutational Analysis of p14ARF 64 2. 5.4 2. 6 RESULTS IN PRIMARY INVASIVE BREAST CARCINOMA 2. 4 .2 2.5 55 2. 4.1 ... steady increase in incidence has been attributed to changes in lifestyle, increasing affluence and an increase in the proportion of women in the industrial workforce in Singapore These changes in ... of p53 in breast carcinogenesis 25 p14ARF-hdm2-p53 REGULATORY PATHWAY 26 1.6.1 hdm2 26 1.6 .2 p14ARF 28 1.6.3 1.6 The p14ARF-hdm2-p53 pathway 30 1.7 E2F TRANSCRIPTION FACTORS 32 1.8 HYPOTHESIS...

Ngày tải lên: 17/09/2015, 17:20

... of institutional factors and market forces in determining the properties of earnings and accounting quality In attempting to hold standards constant and to isolate the influence of reporting incentives, ... Scandinavian 10.00 0.64 United Kingdom Mean private public private public 12 12 1 52 122 23 ,786 20 9,665 25 ,969 390,1 52 11.8% 14.7% 8.8% 12. 5% 9 .22 0.86 ** 4.1% 5.8% 3.3% 5.1% English ** 43 .2% 28 .4% ... 44.4 39.6 Finland private public 6,734 350 11 10 2. 991 0.700 0. 526 0.385 -0.680 -0.654 0. 822 0.741 36.8 22 .6 France private public 47,4 82 1 ,28 2 12 12 3.554 2. 667 0.677 0.519 -0. 622 -0.757 0.885...

Ngày tải lên: 06/03/2014, 21:20