the dictyostelium rho family of proteins and effectors

... 7A–C) Therefore, the deletion of BACE1, and consequently the loss of BACE1 processing of APP, APLP1 and APLP2, had no detrimental effect on the de novo production of ICDs (compare lanes and and ... expressed, and its subcellular localization and dimerization properties are different from those of APP and APLP2 [16] Given the similarities between processing of Notch and of the APP family of proteins, ... together with associated proteins, are released from the membrane [49] Here we investigated the effects of genetic manipulation of BACE1 on the processing of APP, APLP1 and APLP2, and on the...

Ngày tải lên: 15/03/2014, 10:20

... calreticulin-negative regions of the ER and co-immunoprecipitates with the SNARE proteins syntaxin 1, syntaxin 7, syntaxin 13 and VAMP2 [39] Overexpression of a fragment of RTN1C increases the rate of exocytosis ... aspects of our knowledge of the reticulon protein family remain incomplete There is no consensus on the mechanism(s) underlying the ER-associated function of reticulons, and debate continues over the ... reticulons and their function There is growing evidence that reticulons are involved in bending and shaping the ER membrane, in trafficking of material from the ER to the Golgi apparatus, and in...

Ngày tải lên: 14/08/2014, 08:20

... represent the pI values of the major bands in e and f, while the numbers at the left are the pI values of standard proteins The arrow at the right represents the pI value of 6.84 calculated for the ... protein and member of the fasciclin I family of proteins These results support the hypothesis that CL in Artemia embryos is unique and functions outside lysosomes, in the cytoplasm and extracellular ... pI standards and column buffer only were prepared After the proteins reached their equilibrium positions, the gels containing the CLAP e, f, pI standards, and buffer only were removed from their...

Ngày tải lên: 07/03/2014, 16:20

... above the relevant lanes, and the absence of any added protein is indicated by ()) The position of the free RNA and of the loading slots is indicated to the right of each gel A B abolished by the ... series of GFP fusions with the various motifs of Roquin, and by selectively deleting domains from the full-length protein, we determined that the ROQ domain in the N-terminus of the protein is the ... indicate this On the left side of the gel, bands in the Icos mRNA probe ladder are aligned to the Icos target RNA (B) Nucleotide sequence of Icos-specific RNA used in the REMSA (Fig 6) and RNase footprinting...

Ngày tải lên: 15/03/2014, 11:20

... assembly and activity of the complex is further enhanced by the addition of a and b domains [50] Mutagenesis of individual residues in PDI confirmed the importance of the a and a¢ domains in the assembly ... Leu508 of the x-linker and residues Phe484, Phe499 and Phe503 of the b¢ domain Towards the middle of the x-linker, Tyr364 of ERp57 (and Val511 of ERp72) fit into a small hydrophobic pocket below the ... conformations in the structures of the isolated b¢ domain and full-length proteins In the crystal structure of the b¢x fragment of human PDI, the C-terminal part of the x-linker turns back to contact the...

Ngày tải lên: 15/03/2014, 23:20

... Detection of C-type heme Because of the sequence similarity of SACCP to members of the BCCP family of proteins and the prediction of heme-binding motifs in the primary sequence, it was of interest ... Both these proteins are members of the BCCP family of proteins [13] (pfam 03150), but are representatives of two functionally distinct subsets of the BCCP family [14] To further explore the relationship ... located in the core of the CCP structure (Fig 3A) Furthermore, all of the additional insertions of the extended MCA2590 sequence are introduced in loop regions on the surface of the structure, thereby...

Ngày tải lên: 23/03/2014, 11:20

... property βi,j is the partition function of the substring si, j, i.e the sum of weights of all possible foldings of si, j The property βi,j is the sum of weights of all possible foldings of si, j that ... value of the form αi,j reflects the sum of weights of all foldings of si,j that contain a base-pair of the form (q 1) • j, for some index q Î [0, i) A value of the form αi,j reflects the sum of ... be the applik cation of the ⊗ operator between the property βi,q of k the prefix si, q , and the property βq,j of the suffix sq, j (where ⊗ usually stands for +, ·, or ⋀) The combined value μk...

Ngày tải lên: 12/08/2014, 17:20

... with variation in the length of the projection domain and the number of microtubule-binding repeats [8,9] The main forms of MAP2 are MAP2c, which is relatively short, and MAP2a and MAP2b, which ... precursors and then becomes strong about day after expression of neuron-specific tubulin isoform βIII [17] MAP2c is the juvenile isoform and is downregulated after the early stages of neuronal ... phosphorylation of MAP2 [72] Many other protein kinases can phosphorylate MAP2/Tau proteins in vitro, but for most the identity of the targeted residues in vivo and the functional consequences of phosphorylation...

Ngày tải lên: 14/08/2014, 14:21

... endocytosis of these pheromone receptors Sla1p is the adaptor that binds these recognition sequences and recruits the rest of the coat to the cargo 1.2.4 Role of actin dynamics in endocytosis Role of ... activity of actin nucleators The single most important nucleator in the context of the actin patch is the Arp2/3 complex, which localizes to the patch and is the sole nucleator implicated in their ... 1997 and 1999) Other actin-nucleators such as the formins - Bni1p and Bnr1p- play a very minor role in patches The activity of the Arp2/3 complex is activated only in the presence of certain proteins...

Ngày tải lên: 14/09/2015, 08:50

... pair of fragment ions of type c¢ and z¢Æ Subtraction of the m ⁄ z values for the fragments within a given ion series that differ by a single amino acid affords the mass and thus the identity of the ... summary of our findings to date can be found on the website http://www.cellmigration.org (first select the CMC Activity Center subheading and then select the Proteomics link) One of the proteins of ... (B) The observed ions of type c¢ and z¢Æ define sequences at the N-terminus and C-terminus of the protein, respectively These sequences match to the 50S ribosomal protein, L34 Lines above and...

Ngày tải lên: 18/02/2014, 16:20

... to the P citrinum KER [14], the S cerevisiae YPR1 [15] and the S cerevisiae GCY1 [16] From their kinetic properties, all these proteins can be categorized as EC 1.1.1.72 Another group of proteins ... from the genomic DNA We called the genes gld1 and gld2, respectively Comparison of the cDNA of gld1 with the genome sequence revealed that the genomic DNA indeed contained three introns The intron ... nucleotides 327, 510 and 916 of the open reading frame and contained 70, 69 and 62 nucleotides, respectively The sequence of the open reading frame codes for a protein with 331 amino acids and a calculated...

Ngày tải lên: 19/02/2014, 06:20

... between a set of strings of terms on one hand and a set of trees on the other (by means of a linear sequence of sets of trees) The rule RN! describes a correspondence between a single term and a tree ... between the whole TREE and the whole STRING (trivial), between the subtree 4(5(6),7) and the two occurrences of the substring "dog catcher" (nontrivial), etc We shall the same in the rest of this ... than the classical definition) The set of rules in a grammar forms a formal grammar, ie it defines a language, in fact two languages, one of strings and the other of trees At the moment, there...

Ngày tải lên: 22/02/2014, 10:20

... signed the Army Family White Paper The Army Family It provided for the annual Army Family Action Plan, the Army theme for 1984 (“Year of the Family ), and the establishment of installationbased Family ... 2,100 At the start of the War of 1812, about 80,000 volunteers and militia augmented the regular Army of 6,744 At the start of the Mexican War, the regular Army was 8,349, and at the start of the ... Defense, the service chiefs of staff, and the head of the Joint Chiefs all still favored the continuation of a mixed system of professionals and draftees It was reported that the head of the Navy...

Ngày tải lên: 06/03/2014, 20:20

... technologies, for the exercise of the right to freedom of opinion and expression, including the right to seek, receive and impart information and the relevance of a wide diversity of sources, as ... of the European Parliament and of the Council on combating the sexual abuse and sexual exploitation of children and child pornography 10 The Special Rapporteur also participated in a series of ... blocking and filtering, to inadequate guarantees of the right to privacy and protection of personal data, which inhibit the dissemination of opinions and information The Special Rapporteur is of the...

Ngày tải lên: 06/03/2014, 21:20

... 3222 in the presence or absence of tunicamycin Drug treatments resulted in the accumulation of forms of the mutant proteins that were of lower apparent molecular mass and of similar size to the unglycosylated ... confirmed that the two proteins are capable of interacting [33,34] The nature of these interactions differs from those observed between HFE and TfR1 in that they are mediated by the a3 domain of HFE, ... mutant forms of HFE and incubated the cells in the presence and absence of tunicamycin Drug treatment resulted in the accumulation of an unglycosylated lower molecular mass form of the wild-type...

Ngày tải lên: 06/03/2014, 22:21

... (e) The reaction mixture is accessible This allows the synthesis of proteins in the presence of other proteins provided in excess at the start of or during the reaction, e.g., for the purpose of ... spectra of five combinatorially 15N-labelled samples of the C-terminal 16 kDa domain of the E coli DNA polymerase III subunit s (A) Overview of the spectra Numbers in the top left corner refer to the ... combinations of [15N]-labelled amino acids The most abundant amino acids are labelled in only one of the samples, while the least abundant amino acids are labelled in up to three of the samples The pattern...

Ngày tải lên: 07/03/2014, 12:20

... minor initial role of monetary policy, the impact of the lagged values of the output gap on the aggregate demand is remarkable, implying that the response of the aggregate demand to the monetary policy ... equation, the lag coefficients of the output gap and of the terms of trade lagged one period were statistically significant (see Table 2) On the other hand, the coefficient of the real interest rate ... that the current inflation rate depends on its lagged values, on the fluctuation of the exchange rate in the previous period and on the two-period lag of the output gap The verticality of the...

Ngày tải lên: 15/03/2014, 14:20

... membranes in vivo [22] On the other hand, colocalization of VCP and PtdIns-4-kinase IIa on the buoyant subfraction of ER-derived membrane was observed [66] The ND of VCP, rather than the D1 or D2 domains, ... PIP Strip assay of the phospholipid binding of the glutathione-S-transferase (GST) fusion protein with the other N-terminal domains of type II AAAATPases and the related adaptor proteins This material ... recognition of the phosphate group The importance of the conserved arginine residue in the molecular recognition of phosphoinositides in other phospholipid-binding domains, such as FYVE [52] and PX...

Ngày tải lên: 16/03/2014, 12:20

... Journal of Science, Mathematics - Physics 25 (2009) 123-128 here, nD is concentration of impurities, m and e are the effective mass and the charge of the electron, respectively and en are the energy ... here V, ρ , ν s , and ξ are the volume, the density, the acoustic velocity and the deformation potential constant, respectively χ is the electronic constant, χ ∞ , χ are the static and high-frequency ... (10) n where, the quantity δ is infinitesimal and appears due to the assumption of an adiabatic interaction of the electromagnetic wave (EMW) In Eq.(8), the first term on the right-hand side is...

Ngày tải lên: 22/03/2014, 11:20

... mass than that produced by the treatment of type II We scanned the western blots in Fig 5A and analyzed the densities of the two bands We found the ratio of band I to band II was 39 : 61 To confirm ... confirm the presence of fusion protein at 66.5 kDa The density of the particles obtained from both cell extracts and the conditioned media was equivalent to that of the BNC Whether from the conditioned ... clarify the results of previous studies of the topology of the HBsAg protein It is suggested that the C-terminus of the envelope protein protrudes from the particle in 1987 [12] Localization of HBV...

Ngày tải lên: 23/03/2014, 15:20