the dark side of the moon revealed

... association of these molecular chaperones with substrates will foster disease Therefore, this review considers the role of sHSPs in several human medical conditions and it ends with a discussion of their ... occurring either as a result of mutation or by post-translational changes, and these are outlined below and described in the text of the review In addition, changes in the amounts of sHSPs, unaccompanied ... Among the three cancers, sarcoma cells possess the most Hsp25, the rodent equivalent of human Hsp27, and the protein builds up upon drug treatment, suggestive of a role in cell survival In another...

Ngày tải lên: 19/02/2014, 18:20

... activity of MTB HSP 16.3 and aB-crystallin In Fig 3, we observed the effects of different concentrations of MTB HSP 16.3 in the presence and absence of ATP on the aggregation of CS In the absence of ... aB-crystallin, the present results demonstrated that ATP enhanced the chaperone effect of MTB HSP 16.3 by twofold in the CS aggregation assay However, the specific role of ATP in the chaperone function of ... Aggregation of CS plotted in arbitrary units against time in the absence and presence of ATP at two different molar concentrations of MTB HSP 16.3 In the presence of ATP, the molecular chaperone effect of...

Ngày tải lên: 23/03/2014, 21:21

... electrophoresis The quantity of actin in the pellet was plotted against the total quantity of actin in the sample Results Heat denaturation of actin Before starting the investigation of the HSP25–actin ... peak at 7.65 mL on the elution profile Increase of the temperature of incubation was accompanied by the simultaneous increase of the peak eluted at 7.65 mL and decrease of the peak eluted at 8.65 ... fifth of the volume of buffer S was added and after mixing the samples were immediately subjected to ultracentrifugation The quantity of actin in the pellet is plotted against the total quantity of...

Ngày tải lên: 20/02/2014, 23:20

... in the absence of the sHsp This may reflect the formation of very large aggregates of MDH that also include the sHsp, as evidenced by the loss of sHsp from the SEC profile under these conditions ... than the TaHsp17.8C-II particles, which have an estimated diameter of only nm Therefore, the relative sizes of the two oligomers are consistent with their behavior on nondenaturing PAGE and SEC Their ... assay, the ratio of sHsp to substrate that is required to suppress light scattering can be used as a measure of effectiveness of the chaperone Using this assay we tested the relative activity of the...

Ngày tải lên: 07/03/2014, 15:20

... assemblies A transfer of CS and HspH into the void volume, at the expense of the other fractions, was confirmed by SDS-PAGE (Fig 7C) Neither HspH nor CS shifted into the void volume when they were heat-treated ... solid line) The second peak of a sample with a concentration of mgÆmL)1 eluted at % 200 kDa at room temperature Preincubation of the protein at 40 °C did not result in a shift of the peak when ... the major species in the spectrum Representative charge states of the associated oligomers (XmerY+) are shown NanoESI-MS was used to confirm the effects of concentration and temperature upon the...

Ngày tải lên: 23/03/2014, 12:20

... nuclear bodies, but the combination S19D/ S45D had the strongest effect The largest number of positive cells was obtained with the S19D/S45D/S59D mutant These results confirm the correlation between ... Western blotting with the anti(aB-crystallin) and anti-S45p IgGs on the isolated nuclei of these cells It appears that only a tiny proportion of the total aB-crystallin is present in the nuclear fraction ... Western blotting using the RIKEN anti-(aB-crystallin) monoclonal antibody (B) The average level of aB-crystallin in the detergent-insoluble fraction is shown as a percentage of the total aB-crystallin...

Ngày tải lên: 23/03/2014, 13:20

... arrangement of sHsps 5886 The alteration of the properties of the C-terminal extension also leads to significant changes to the structure and function of sHsps The chaperone activity of Hsp30C is ... examined the role of residues in the C-terminal extensions of other sHsps, notably aA- and aB-crystallin, the flexible regions of these proteins are unique and distinct from that of Hsp25 The a-crystallins ... maintaining the solubility of the Hsp25 oligomer Similarly, the introduction of hydrophobicity into the C-terminal extension of aA-crystallin results in a decrease in the thermostability of this...

Ngày tải lên: 07/03/2014, 04:20

... were excluded Application of procheck [76] revealed that the stereochemical quality of the model was reliable, with 84% of the residues in the most favored regions of the tetramer model and none ... ovotransferrin, with the ratio rising as the molecular mass of the substrate increases [58] For human aB-crystallin, the ratio is : [48] The p26 chaperone activity therefore approximates that of other sHSPs ... between strands b2 and b6 of neighboring monomers The p26 modifications examined in this study are not located in either of these strands and they are not considered further As the basic p26 oligomer...

Ngày tải lên: 07/03/2014, 12:20

... destabilization of the overall structure of the sHsp [35,36] Therefore, we analyzed the effect of point mutations in the linker connecting the b5 and b7 strands of HSP22 on its thermal stability The wild-type ... correspond to the b8 strand of the other sHsp The primary structure of the a-crystallin domain of human sHsp is very conservative and the loop connecting the b5 and b7 strands is shorter than the corresponding ... affect the elution profile of any of the proteins analyzed The amplitude, position and the width of the protein peaks were not dependent on the transient heating These data suggest that the wild-type...

Ngày tải lên: 23/03/2014, 07:20

... development of the lens and maintenance of the olfactory epithelium [102] The first evidence for a developmental function of HSF4 was provided by population genetic studies where mutations of the Hsf4 ... how the developmental activity of HSF1 is regulated The identity of the developmental signal that promotes a monomer-to-trimer transition of HSF1 in the olfactory epithelium warrants further ... In accordance with the fundamental role of HSF1 in the heat shock response, the requirement of HSF1 and HSF4 in development of the lens and olfactory epithelium is limited to the postnatal period,...

Ngày tải lên: 18/02/2014, 04:20

... distribution of the size of the inclusions was noticed Moreover, the percentage of cells presenting inclusions as well as the distribution of the size of the inclusions were dependent on when the analysis ... (A) The percentage of the cell population with an FL2-H fluorescence greater than · 101 was recorded during the FACS analysis The percentage of decrease of DYm was calculated as the ratio of the ... analyzed The arrow indicates the position of the more intensively oxidized polypeptide in the assay The bracket underlines the domain (molecular mass range 10–40 kDa) of the oxyblots that contains the...

Ngày tải lên: 19/02/2014, 06:20

... amplified only the cDNA in p26-full, and amplification of constructs with the primers employed for production of their respective inserts yielded PCR products of the expected length That is, the fragments ... Neither of these changes modified the deduced amino-acid sequence of p26 The p26 cDNAs cloned in pET21(+) and utilized in subsequent experiments are represented schematically in Fig Synthesis of ... to the His6 epitope tag The top of each gradient is to the left of the figure, and numbers across the top indicate successive samples from gradients A, p26-full, B, p26-ND60; C, p26-alpha The...

Ngày tải lên: 22/02/2014, 04:20

... analyzing the distribution of the charge residues in the C-terminal region of Hsp20, and by calculating the theoretical pI values of differently truncated species of Hsp20, we found that cleavage of the ... arrows (B) Rearrangement of the complexes formed by the 3D mutant of Hsp27 and the wild-type Hsp20 A mathematical summation of the elution profiles of the isolated 3D mutant of Hsp27 and isolated ... L of the E coli culture When the S16D mutant of Hsp20 was expressed in E coli, most of the protein was insoluble in the lysis buffer and this buffer extracted less than 20% of the protein The...

Ngày tải lên: 07/03/2014, 15:20

... In the absence of Hsp-3D, thermal denaturation of F-actin led to the formation of very large aggregates with Rh up to 10 lm (Fig 2B) In contrast, in the presence of Hsp27-3D, the F-actin thermal ... formed during the course of F-actin thermal denaturation All the methods used here show that the size of these complexes depends on the Hsp27-3D ⁄ actin ratio in the initial mixture of Hsp27-3D ... together, all these results support a viewpoint that the formation of soluble complexes with non-native proteins is a conserved feature of the sHSP family of chaperones, and the morphology of these...

Ngày tải lên: 16/03/2014, 06:20

... intensities, with the peak of G and TS at 348 nm and R at 360 nm The fluorescence intensity of R was the lowest and it was red-shifted in comparison to other samples The extent of tertiary structure ... decreasing order of activity Although none of the mutants with internal deletions completely inhibited the aggregation of citrate synthase at 600 nm, even the least effective chaperone offered significant ... and the other in arginine Additionally, the C-terminus contains an unusual stretch of residues endowed with threonine and serine These regions are interesting because the characteristics of different...

Ngày tải lên: 30/03/2014, 20:20

... as one of the three main consensus regions of the a-Hsp family [5] There is little experimental evidence to circumstantiate the role of the C-terminal extension, and even less concerning the conserved ... isoleucine residues Two further constructs, HspH(D5C) and HspH(D15C), contained the entire I-X-I motif To assess the role of the conserved motif in the context of the full-length protein, three ... Absorbance of a-Hsp proteins in the absence of CS is also shown (S) consisting of the a-crystallin domain alone [HspH(a)], the a-crystallin domain plus the N-terminal region [HspH(Na)], or the a-crystallin...

Ngày tải lên: 31/03/2014, 21:21

... of inflammatory cells in the urinary bladder of schistosomal patients may enhance the carcinogenic potential of these agents by increasing their rate of activation Furthermore, in patients with ... that the "so-called small cellular infiltration [of Virchow] of the connective tissue was the 'pre-stage of cancer"' [16] Since that time, the study of inflammation has become increasingly com- Lipid ... iNOS in the gastric mucosa by attenuating the expression of HSP70 and HSP27 Tumor-associated macrophages (TAM) are not toxic to tumor cells because of their limited production of NO Page of 10...

Ngày tải lên: 10/08/2014, 22:20

... characteristics of SPNPs to improve the efficiency of HSPs 72 induction c Identification of the induction of HSPs 72 d Investigation of the correlation between the change of AC heating stress and the behavior ... Investigation of the physical contribution of Néel and Brown relaxation loss power to the AC heating power of fluidic SPNPs e Understand the physical mechanisms of the AC heating and the crucial ... example of equipment of superficial and interstitial hyperthermia (a) Superficial microwave hyperthermia of malignant melanoma of the skin, (b) interstitial hyperthermia in a radical treatment of...

Ngày tải lên: 09/09/2015, 10:08

... the absence of HSP or in the presence of a control protein, lactoferrin The competence of the heat shock proteins (HSPs) to promote antigen cross-presentation was unaffected in the presence of ... investigate the role of the HSPs in antigen presentation and signaling Furthermore, we examine the immunogenicity and regulatory properties of HSPs using an in vivo mouse model In view of the issue of ... However, due to the low levels of IL-2 secreted, the data is inconclusive The role of B pseudomallei Hsp70 and Hsp60 in the presentation of the short Ova peptide SIINFEKL To investigate whether Hsp70,...

Ngày tải lên: 09/10/2015, 11:06