the california stem cell research and cures initiative

... population of stem/ progenitor cells in the breast cancer (MCF7) cell line [11] The fact that the vast majority of MCF7 stem cell enriched SP cells express MUC1 suggests that epithelial cancer stem cells ... ESA and ALDH1 in different cancers [1] Few of these markers were used for the confirmation of an MUC4 enriched cancer stem cell population Stem cells and cancer stem cells are known to possess the ... HER2 and the cancer stem cell population in MUC4 overexpressed ovarian cancer cells Further, we have analyzed cancer stem cell and self-renewal specific markers in the isolated populations These...

Ngày tải lên: 20/06/2014, 07:20

... of understanding the functioning of these remarkable stem cells However, current research in stem- cell biology is providing increasing evidence that the behaviors and fates of stem cells are ... models for stem- cell research They present a number of advantages, including the fact that a large pool of stem cells is available throughout the lifetime of each individual; the stem- cell pool ... of the Bilateria (namely, the Deuterostomia, Lophotrochozoa, and Ecdysozoa) Whether their similar stem- cell systems are homologous or convergent remains an open question The biology of these stem...

Ngày tải lên: 06/08/2014, 19:21

... 2cm from the top of the plate, then removed from the chamber and air dried in a fume hood Radioactive signal on the TLC plate was captured by exposing the plate to a TyphoonTM scanner and the intensity ... fact, in the concentrations tested (10μM, 25μM, 50μM, 75μM, and 100μM), none of the six compounds inhibited DAGK and human PKC alpha (Figure 2.8 and Figure 2.10) On the other hand, and in agreement ... of the assay (~5-25μg/mL), the more protein present, the more the Coomassie Blue binds changing the absorbance of the sample After getting cell lysates from the CHO cells, over-expressing SPHK1...

Ngày tải lên: 11/09/2015, 16:06

... hypothesized that when suppressing SPHK activity in the stem cells, the balance between proliferation and differentiation could be lost, the cell growth was arrested, and the cells would then ... 3.1.1.3 Stem Cells Seeding Density Unless otherwise stated, stem cell seeding density used was 5,000cells/cm2 in all of the experiments using human BM- and AD- MSCs 3.1.1.4 Stem Cells Osteogenic and ... penetrate into the cells and inhibit the endogenous SPHK1 and the good yield obtained from the chemical synthesis procedure Therefore, in this chapter, CP6 was selected to study the function of...

Ngày tải lên: 11/09/2015, 16:06

... that the stem cells remained as stem cells Starting cells for human BM-MSCs were passage 6, and for human AD-MSCs (cells from two different donors) were passage Cells were equally grouped and ... passage they were considered to maintain their multipotency Cells cultured in S1P-supplemented media were then measured for their stemness maintenance by checking their morphology change, the cell ... promote stem cell proliferation (Figure 4.5, Figure 4.6, and Figure 4.7) The cells were washed in PBS and starved in serum-free DMEM for half an hour before 1μM S1P was added into the cells and incubated...

Ngày tải lên: 11/09/2015, 16:06

... study other stem cells and other differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors in stem cell differentiation, and S1P roles on stem cell proliferation ... tissue-specific stem cells (i.e MSCs), the knowledge accumulated on cell expansion and differentiation might also be applicable in other types of stem cells Also, it should be noticed that both human BMand ... primary cells, and thus they may possess certain variations between different donor cells Therefore, more work need to be done to further confirm our conclusions Finally, the study could be expanded...

Ngày tải lên: 11/09/2015, 16:06

... directing the differentiation of stem cells into the osteogenic lineage in vitro.” J Bone Miner Res 19:1379–1394 Hentze, H, Graichen R, et al (2007) Cell therapy and the safety of embryonic stem cellderived ... platelet-derived growth factor in the maintenance of human embryonic stem cells." Stem Cells 23(10): 1541-8 Peng, H and J Huard (2003) "Stem cells in the treatment of muscle and connective tissue diseases." ... stem cells." J Cell Biochem 101(5): 1238-51 Sotiropoulou, P A., S A Perez, et al (2006) "Cell culture medium composition and translational adult bone marrow-derived stem cell research. " Stem Cells...

Ngày tải lên: 11/09/2015, 16:06

... human stem cells and the strategies used for stem cell expansion and differentiation, are discussed below 1.4 HUMAN STEM CELLS 1.4.1 Brief Introduction of Human Stem Cells Diverse stem cells and their ... On the one hand, it could act as an intracellular mediator and trigger numerous cellular events, including intracellular calcium release, and promote cell survival and cell growth On the other ... Objectives and significance The significance for the need to study the potential applications and significance of using SPHK and S1P in stem cells research, derived from the current understanding...

Ngày tải lên: 11/09/2015, 16:06

... locus are the pre-microRNA hairpins and the putative minimal promoter The number and precise sequences of the pre-microRNAs, their distance from the promoter and the polyadenylation sites, the regions ... ESC-based systems stems from this potential of ESCs to differentiate into all cell types in the body Although the clinical use of adult stem cells, which are present in multiple tissues in the mammalian ... (Erlandsson and Morshead, 2006; Mimeault and Batra, 2006; Serakinci and Keith, 2006) These properties limit their use as a scaleable, continous resource for generating multiple cell types for cell- based...

Ngày tải lên: 12/09/2015, 08:16

... begin to form the placenta, and the inner cell mass would begin to form the cells and tissues of the fetus At the blastocyst stage, cells of the inner cell mass are undifferentiated and pluripotent; ... fundamental and extraordinary of the stem cells; unlike the more differentiated adult stem cells or other cell types, they are pluripotent (See the glossary for terminology used in this report.) The ... (the blastocoel), and a cluster of cells in the interior (the inner cell mass) Up to this point, there has been no net growth (Figure 2.1) The cells of the inner cell mass will give rise to the...

Ngày tải lên: 06/03/2014, 15:20

... scientific and engineering research, dedicated to the furtherance of science and technology and to their use for the general welfare Upon the authority of the charter granted to it by the Congress ... protecting SCADA systems, the EPA should consider the differences between the largest and more sophisticated systems and the vast majority of the systems which are quite small and may have limited ... summarizes the early findings of the Panel on Water System Security Research, which is overseen by the NRC’s Water Science and Technology Board The first task for the Panel on Water System Security Research...

Ngày tải lên: 06/03/2014, 15:20

... reported the isolation of a type of precancerous stem cells from dendritic cell- like leukemic mice and the establishment of this precancerous stem cell line The precancerous stem cells had stem cell- like ... cancer stem cell hypotheses, cancer stem cells are ideal seeds of metastasis Stem cells are indeed ideal carrier of gene mutations and their accumulation First, the initiating cell must be a cell ... will focus on the origin and the fate of primary cancer stem cells Precancerous stem cells Based on current literature, primary cancer stem cells may be derived from precancerous stem cells Chen...

Ngày tải lên: 18/06/2014, 19:20

... reported the isolation of a type of precancerous stem cells from dendritic cell- like leukemic mice and the establishment of this precancerous stem cell line The precancerous stem cells had stem cell- like ... cancer stem cell hypotheses, cancer stem cells are ideal seeds of metastasis Stem cells are indeed ideal carrier of gene mutations and their accumulation First, the initiating cell must be a cell ... will focus on the origin and the fate of primary cancer stem cells Precancerous stem cells Based on current literature, primary cancer stem cells may be derived from precancerous stem cells Chen...

Ngày tải lên: 20/06/2014, 03:20

Ngày tải lên: 20/06/2014, 21:20

Ngày tải lên: 21/06/2014, 17:20

Ngày tải lên: 21/06/2014, 19:20

... sections: 1) Hematopoietic stem cell properties, 2) Regulation of hematopoietic stem cells, 3) Hematopoietic stem cells in aging and disease, and 4) Hematopoietic stem cell therapy The first section provides ... super-physiologic increase in stem cells in the absence of OPN Therefore, OPN seems to be a negative regulatory element of the stem cell niche that limits the size of the stem cell pool and may provide a ... self-renewal and C) survival 18 Advances in Hematopoietic Stem Cell Research In part, the dramatic contrast in cell cycle status between stem and progenitor cells has led to the hypothesis that cell...

Ngày tải lên: 27/06/2014, 13:20

... subcellular domains Epithelial cells are the classical polarized cell in which specific receptors are largely confined to either the apical or basal surface, and signals received at these subcellular ... microfluidics-based cell- culture systems, and the ease with which fluid flows can be controlled over time and space Human embryonic stem cells (hESCs) are particularly sensitive to handling in culture, and automation ... translational stem cell biology can be addressed using microfluidics tools? Here again, microfluidics techniques afford the ability to define the microenvironment surrounding stem cells The disease...

Ngày tải lên: 06/08/2014, 19:21

... genotype CD4+ CD8+ cells appear at the upper right quadrant and are 31% of the total cell numbers Note also the very low numbers of CD4+ and CD8+ cells which appear in the lower right and upper left ... the mice, mating, genotyping, embryo cells isolation and culture FS and LZ and GZ carried out the comet assay, transformation assays, apoptosis and micronuclei assay EJH conceived the study and ... and plated at the same density The clones which seemed dense and had stellate-shaped piled cells were photographed and isolated with cloning cylinders These clones were expanded and injected into...

Ngày tải lên: 09/08/2014, 08:22

... carried out the flow cytometric analysis FM, AO, and KN participated in the design and coordination of the study MM, HI, and RS participated in the design of the study and the maintenance of the animals ... including systemic lupus erythematosus We previously reported that B cells lacking RP105 expand in the peripheral blood of patients with systemic lupus erythematosus [32] and that these cells can ... cocktail and further incubated with anti-biotin microbeads and phycoerythrin-labeled anti-CD25 antibody After washing, the cells were applied to the LD (lym- phocyte depletion) column, and the T cells...

Ngày tải lên: 09/08/2014, 13:22