Ngày tải lên: 13/08/2014, 01:21
Báo cáo khoa học: DNA-binding characteristics of the regulator SenR in response to phosphorylation by the sensor histidine autokinase SenS from Streptomyces reticuli doc
... R1NSph (5Â-CAGCGC ATGCTGCTCCAGGCAGCCGAC-3Â), and one of the reverse primers R1D65Pst (5Â-CGAGCTGCAG GGCCAT CAGGACGACGTC-3Â) or R1D60Pst (5Â-CGAGCTGCAG GTCCATCAGGACGAC GGCCGGGGCGGTCTTGC-3Â). The PstI ... 5Â-CCCATATGACCCCCACCCCGCAGCCG CC-3Â, consisting of an NdeI restriction site (in bold type), followed by the sequence encoding the N-terminal amino acids of SenR, and SenR2, 5Â-CGCGCTCGAGAGACAGG AGGCGTTGTTC-3Â, ... and characteristics, see above II ⁄ IIIEcorev GGGAATTCGGGCCGAGGATCGGTTCCGGGAGCGACCGACGCCCCCTCCTCAGCATGTCC (located upstream of hbpS and ending at the 5Â -end of binding site III, and containing...
Ngày tải lên: 07/03/2014, 10:20
The Education Of The Negro Prior To 1861 - A History of the Education of the Colored People of the United States from the Beginning of Slavery to the Civil War pdf
... due to the high character of the colonists, to the mutual aid resulting from the proximity of the communities, and to the coöperation of the Canadians. The previous experience of most of these adventurers ... to be incompetency and liability to abuse their office and influence to the injury of the laws and peace of the country. The elimination of the Christian teachers of the Negro race, and the prevention ... maintain schools despite the fact that the fear of servile insurrection caused the State to exercise due vigilance in the execution of the laws. The father of Richard De Baptiste of Fredericksburg...
Ngày tải lên: 31/03/2014, 18:20
the roots, verb-forms and primary derivatives of the sanskrit language
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Ngày tải lên: 04/05/2014, 14:27
Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... nonspeci c hydrolysis, of the polypeptide chain. When investigating the effect of pH on the cyclization reaction, we expected that an acidic pH might enhance the rate of conversion of (Gln1)-ONC(M23L) ... that the procedure described in this work for the activation of ONC has no detrimental effect on the conformational stability of the enzyme. Cytotoxic activity The effect of (Met1) removal on the ... by MALDI-TOF MS. The measured molecular mass of the purified product was 11 799.70 ± 2, which concurs closely with activated (Pyr)-ONC (M23L), which has a theoretical molecular mass of 11 801.60. Contribution...
Ngày tải lên: 19/02/2014, 12:20
Structure of the S15,S6,S18-rRNA Complex:Assembly of the 30S Ribosome Central Domain
... where ͉f calc ͉ is the calculated heavy-atom structure factor amplitude for centric reections. The phasing power is the ratio of the mean calculated heavy-atom structure factor amplitude to the mean ... birefringence studies, and the conformation of the bound junction is clearly seen in the structure of the Tth T4 RNP (Fig. 2C) and in the structure of the 30S subunit (1). Biochemical studies of the ... packs tightly with the other helices, similar to the nuclear magnetic resonance structure of the free protein (19) but unlike the crystal structure of the free protein (20), in which ␣1 lies distal...
Ngày tải lên: 13/03/2014, 19:08
Báo cáo khoa học: The propeptide of cruzipain ) a potent selective inhibitor of the trypanosomal enzymes cruzipain and brucipain, and of the human enzyme cathepsin F ppt
... cruzipain Complementary oligonucleotides directed to residues Cys19 (5Â-GCGGATCCTGCCTCGTCCCCGCGGCG-3Â) and Gly122 (5Â-CGCCCGGGGCCAACAACCTCCACG- TC-3Â), which span the full-length predicted prodomain of the ... prostate cancer cells derived from brain metastasis of human prostate cancer were obtained from the ATCC, and human primary cultures of smooth muscle cells (Cell Bank of Rio de Janeiro) were cultivated ... His-tag); (b) the six histidines of the tag; (c) the full-length propeptide of cru- zipain (Cys19–Gly122); and (d) residues RGVDLQPS- LIS at the C- terminus, which resulted from the fusion of the...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc
... 5Â-TGGAATCCATATGCCGAG CCCGCAGCTTCTG-3Â and 5Â-GGAATTCCGGATCC TTAGGGCAGGGGGACTCC-3Â as forward and reverse primers, respectively. Following amplification, the PCR product was cloned into pCR Blunt (Invitrogen) ... concentration of the FMN domain is increased indicates that the reaction rate is controlled by some process other than collision of the two flavin-binding domains. Discussion The ability to dissect ... this domain. The presence of the His- tag on the FAD/NADPH domain had no apparent effect on catalytic activity. The purified domains were yellow, indicating the presence of bound cofactor, and they...
Ngày tải lên: 23/03/2014, 21:20
Báo cáo khoa học: Expression, purification and characterization of the second Kunitz-type protease inhibitor domain of the human WFIKKN protein pot
... with the 5Â-GAG TCG ACC GAC GCC TGC GTG CTG CCT GC-3Â sense, and 5Â-GCA AGC TTA CGG CAC GGG GCA GGC ATC CTC-3Â antisense primers from a plasmid containing the cDNA coding for WFIKKN protein. The ... Results and discussion Structural characterization of the recombinant Kunitz-module of human WFIKKN protein The circular dichroism spectra of the second Kunitz-module of WFIKKN protein (hereafter ... inhibitor module of human WFIKKN. The solid line indicates the spectrum of the recombinant protein, the dotted line indicates the CDPro-predicted spectrum of a protein consisting of 0.051 regular b-strand,...
Ngày tải lên: 31/03/2014, 01:20
Báo cáo hóa học: " Comparison of the self-administered and interviewer-administered modes of the child-OIDP" ppt
... analysis and participated in writing the manuscript, KOB participated in the study design and critically reviewed the manuscript, AS conceived of the study and critically reviewed the manuscript, CO ... were all further categorised into 3-point scales for the analysis. Data analysis The analysis started with a baseline comparison of socio- demographic characteristics and self-perceived measures between ... effective than the current face-to-face interview and would further facilitate the applicability of the instrument in both clini- cal practice and population epidemiological survey set- tings. Therefore,...
Ngày tải lên: 18/06/2014, 22:20
báo cáo hóa học:" Revision hip replacement for recurrent Hydatid disease of the pelvis: a case report and review of the literature" doc
... the musculoskeletal system [1-11]. Involvement of the musculoskeletal system occurs in 1% to 4% of all cases [7]. Hydatid disease is a parasitic infection caused by tapeworm Echinococcus whic h ... visible cysts. The acetabular component was loose and it was easily removed. There was a complete loss of posterior column with discontinuity of ilium from pelvis. Curettage and excision of all the ... Grimer Abstract A case of a large recurrent hydatid cyst involving the right ilium and right hip treated with excision of the cyst, Total hip replacement and revision of the acetabular component with...
Ngày tải lên: 20/06/2014, 04:20
báo cáo hóa học:" Repositioning and stabilization of the radial styloid process in comminuted fractures of the distal radius using a single approach: the radio-volar double plating technique" pot
... Kohut Abstract Background: A possible difficulty in intra-articular fracture of the distal radius is the displacement tendency of the radial styloid process due to the tension of the brachioradialis tendon. Methods: ... surface during reduc- tion is necessa ry, a single vo lar approach is contraindi- cated unless sufficient control of the articular surface can be provided by arthroscopy [24]. This can be the case ... radiographic evidence of fracture healing. Figure 1 Rad ial styloid dislocation and reduction. Dislocation of the radial styloid process is favored by the traction of the brachioradialis tendon. The...
Ngày tải lên: 20/06/2014, 04:20
Báo cáo lâm nghiệp: "Verification of the food supply to game under conditions of the floodplain forest ecosystem" docx
... capacity, 0.81% is of considerable carrying capacity, and 0.83% is of low carrying capacity. Numerical stock of game At the end of the last century, there were high stocks of game in the Soutok Game ... the carrying capacity of edaphic categories, so called potential carrying capacity (Table 2). Based on the calculation documented in Table 2, the maximum number of converted units of hoofed ... MJ. To assess the sufficient food amount from the aspect of quality, the need for energy was calculated on the basis of the metabolic Table 2. Calculation of conversion units of hoofed game on...
Ngày tải lên: 07/08/2014, 10:21
báo cáo khoa học: "Primary malignant mixed müllerian tumor of the peritoneum a case report with review of the literature" doc
... 2). Numerous sections from theuterusandtheadnexae showed no evidence of tumor. Immunohistochemistry Immunohistochemical staining for cytokeratin 7 deco- rated cells of the epithelial component and scattered cells ... and the immunohistochemical analysis of our case, we believe that this is a monoclonal tumor with carcinoma being the “precursor” element. Nevertheless, further molecular and genetic evidence is ... Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution,...
Ngày tải lên: 09/08/2014, 01:24
báo cáo khoa học: "Giant Merkel cell carcinoma of the eyelid: a case report and review of the literature" potx
... exact diagnosis of MCC is made with a biopsy, for special stains are used to dis- tinguish. Immunohistochemistry is very helpful. MCC from other forms of cancer, such as sebaceous cyst, small cell ... paranuclear or crescentic pattern. Syn Neurofilament is also expressed in the cytoplasm of most MCC. The above findings support the diagnosis of primary MCC. Diagnosis of MCC involves the following: ... further case of the unusual tumor in the eyelid with histological, pictorial and immunohistochemic al studies, which supports the hypothesis that it is derived from Merkel cells. We consider the...
Ngày tải lên: 09/08/2014, 01:24
báo cáo khoa học: "A male case of an undifferentiated carcinoma with osteoclast-like giant cells originating in an indeterminate mucin-producing cystic neoplasm of the pancreas. A case report and review of the literature" docx
... the pancreatic tail. The patient underwent distal pancreatectomy/splenectomy/total gastrectomy/cholecystectomy. The mass consisted of a multilocular cystic lesion. Microscopically, the cyst was ... of circulating precursor cells to OGCs. Table 1 Clinicopathological findings of UC with OGCs of the pancreas originating in mucinous cystic neoplasms (MCN) and indeterminate mucin-producing cystic ... in the bottom of the solid part of this cystic tumor. (C) Near the carcinoma in situ, OGCs were distributed diffusely in the stroma of the cyst wall. (D) The tumor showed the invasion to the...
Ngày tải lên: 09/08/2014, 02:21
Báo cáo khoa học: "Mucinous cystic neoplasms of the mesentery: a case report and review of the literature" pdf
Ngày tải lên: 09/08/2014, 04:21
báo cáo khoa học: "Adenoid cystic carcinoma intermingled with ductal carcinoma of the breast: a case report and review of the literature" docx
Ngày tải lên: 10/08/2014, 23:20