test for final exon

... Information varies with intron and exon length Information varies with intron and exon length Donor and acceptor sites flanking either long or very short introns or exons have increased information ... average score for the set of sequences S equals the total information for the alignment: Σ{score(s) | s ∈ S}/n = information(1 m) = Σ{information(p) | ≤ p ≤ m} (3) where the information at position ... varied intron (Figure 2a) information Genome Biology 2006, 7:R3 interactions Figure (see following Cumulative informationpage) Cumulative information Cumulative information for positions -32 to +32...

Ngày tải lên: 14/08/2014, 16:20

... Amendt BA, Si ZH, Stoltzfus CM: Presence of exon splicing silencers within human immunodeficiency virus type tat exon and tat-rev exon 3: evidence for inhibition mediated by cellular factors ... 10 (page number not for citation purposes) Retrovirology 2006, 3:10 splicing at 3'ss A1 and A2 also led to either increased or reduced levels of tat mRNA containing exon or exon 3, respectively ... therefore inferences about Tat expression from the 3'ss A1 and A2 oversplicing mutants cannot be made from these assays Viral mRNA stability is not affected by non-coding exon inclusion HIV-1 exon...

Ngày tải lên: 13/08/2014, 09:21

... priori unexpectedly for inefficient splice sites, previous studies have also mapped splicing enhancer sequences as binding sites for SR proteins in exon [23], the HXB2 specific exon [20] and downstream ... level Therefore, the presence of D4 not only has been a prerequisite for splicing but also for the nuclear export of unspliced RNA through the action of Page of 20 (page number not for citation ... were completed with a final elongation step of premature amplified products at 72°C for 10 Accordingly to the obtained results, PCR analysis was performed with 26 cycles for pSV-1-env as well...

Ngày tải lên: 13/08/2014, 09:20

... potential target genes will be useful for future studies of hematopoietic differentiation and tumorigenesis Methods Exon trapping Exon trapping was performed by using an Exon Trapping System kit (GibcoBRL) ... novel exon residing within the gag region of Akv MLV In order to identify potential alternative splice donor and splice acceptor sites in Akv MLV, exon- trapping was performed using the exon- trapping ... and/or fixed in formalin for further analysis Statistical analysis was carried out using the two-tailed Fisher's exact test Histopathological examination and immunohistochemical analysis Formalin-fixed,...

Ngày tải lên: 13/08/2014, 05:22

... $QWL 5HY Figure rtm ORF codes for two 18- and 17-kDa protein isoforms related to envelope precursor and TM proteins rtm ORF codes for two 18- and 17-kDa protein isoforms related to envelope precursor ... [13,14] Thus, Rev is required for virus gene expression and replication The Vif protein acts at the late stage of virus formation and/or release [15], and is required for viral replication in vivo ... immune response against the Rev and/or Env proteins, we tested for the presence of antibodies recognizing the KYQPQIYRT Rtm-specific epitope For this purpose, fifty milliliters of pooled sera from...

Ngày tải lên: 13/08/2014, 06:20

... I Görlich for expert technical assistance, F Liu and Z-G Han for providing clone material, members of the RefSeq Division staff of the National Center for Biotechnology Information for helpful ... than providing a pathway for alternative transcripts or protein isoforms, may play a role as a regulatory bottleneck for maturation of the transcript, as was suggested for U12type introns [39] ... methods Screening for non-canonical 3' splice sites interactions information Genome Biology 2007, 8:R154 refereed research For validation of splice variants, nested PCR was performed using ng cDNA...

Ngày tải lên: 14/08/2014, 08:20

... increasingly on information technologies (IT) for the following reasons First, IT helps the marketer to collect, screen, and analyze all the marketing-related information available before its introduction ... there is a strong demand for a given technology For instance, most of the European high-tech service companies have been practicing this very basic type of marketing for years by simply exploiting ... organize their sales force by market (geographically and by customer type) and assign product managers for the most important products Some companies have organized their sales force and marketing...

Ngày tải lên: 24/10/2013, 07:20

... Building Sample Sites Creating the Registration Form for Institute of Art ! Create a registration form • Create the registration form for Institute of Art The form should contain the following fields: ... contact information for the lecturer, and the duration of the course The second link should contain a registration form that the users can fill in to apply for a particular course This form should ... courses that they are registering for Students can either use Form Page Wizard for creating the form or create it manually When a user submits the registration form, the particulars of the user...

Ngày tải lên: 11/12/2013, 14:15

... • Goods • Services • Money • Information • Interorganizational Information Systems (IOS) Between two or more organizations • Routine transaction processing • Information flow 15 Classification ... Exchange-to-exchange (E2E): EC model in which electronic exchanges formally connect to one another for the purpose of exchanging information 21 e-Business and e-Commerce Overview • Successful e-businesses ... looking for in one place • Horizontal portals • Portals that aggregate information on a broad range of topics • Yahoo!, AltaVista, Google • Vertical portals • Portals that offer more specific information...

Ngày tải lên: 16/01/2014, 17:20

... Selling ad space provides additional income • Monthly charges for online advertising rarely used • CPM (cost per thousand) • A designated fee for every one thousand people who view the site on which ... click-throughs to your site • Pay-per-lead: you pay the host for every lead generated from the advertisement • Pay-per-sale: you pay the host for every sale resulting from a click-through 17 Buying ... Selling advertising space • Provide appropriate contact information on your Web site • Register with organizations that will sell your space for you • These companies typically charge a percentage...

Ngày tải lên: 16/01/2014, 17:20

... Transportation and Shipping 14 Online Automotive Sites • Consumers access automobile information empowering them to make an informed buying decisions • Dealers use the Web to showcase vehicles • Online ... Accountability Act (HIPAA) • Requires medical offices and other healthcare related agencies to perform many of their administrative tasks online • Examples: • WebMD • Sickbay.com • Dr.Koop Medical ... the power to bypass a travel agent • Discounts and low fares available online • Name-your-price for tickets, hotels and car rentals • “Last minute” fares often lower online • Examples: • Expedia.com...

Ngày tải lên: 16/01/2014, 17:20

... mistakes before the end of this one The Errata page on the book’s web site will always have the latest information about known typographical and code errors, and necessary updates for new releases ... never done any server-side programming before, the code may look a little scary But before we analyze it in detail, let’s load the page and test that it works for real To see your dynamically generated ... Director for SitePoint, Kevin Yank oversees all of its technical publica­ tions—books, articles, newsletters, and blogs He has written over 50 articles for SitePoint, but is best known for his...

Ngày tải lên: 14/02/2014, 10:20

... cerebral attack without on that account forfeiting our veneration for the Principia, Comte may have suffered in the same way, and still not have forfeited our respect for what is good in the systems ... trait of magnanimity on Comte's part His character, admirable as it is for firmness, for intensity, for inexorable will, for iron devotion to what he thought the service of mankind, yet offers ... literary form are really astonishing It is hardly possible for a critic to share the admiration expressed by some of Comte's disciples for his style We are not so unreasonable as to blame him for...

Ngày tải lên: 18/02/2014, 12:20

... purified SOD samples stained for SOD activity Lane contains FeSOD and MnSOD markers (from Sigma, arrowed, lg each), lanes 2–9 as for lanes A1 to A8, lanes 10–13 as for lanes C1 to C4 been derived ... purified SOD were incubated with hydrogen peroxide (0.25 mM for FeSODs and mM for MnSODs) at 22 °C At the indicated times, aliquots were removed for analysis These were calculated to give unit of SOD ... Yamakura and T Matsumoto for the analyses of iron and manganese in protein samples We also wish to thank H Steinman for the gift of E coli OX326A We finally thank Mr M Farrugia for photographic assistance...

Ngày tải lên: 21/02/2014, 01:21

... computing a lower minimum value of the predictive performance Obviously, more structural data are necessary to validate our speculation A blind test To test the applicability of this method, we predicted ... conserved threeresidue motif (HPD; for review see [31]) For each of the three structures, the network predicts putative interacting residues on the protein complexes (Fig 4) For the DnaK N-terminal domain ... surfaces in unbound proteins EXPERIMENTAL PROCEDURES Selection of the database The data set for training/testing was selected from the SPIN database (http://trantor.bioc.columbia.edu/cgi-bin/SPIN/),...

Ngày tải lên: 21/02/2014, 15:20

... value for H475A and the Vm.app value for D476N decreased and values for D476E were not different from those of wild-type enzyme D476N and D476E were also tested with testosterone and nortestosterone ... value, for testosterone whilst its binding affinity and the Vm.app value were lower for nor-testosterone The mutant F320C, weakly active with testosterone, was found to have a lower Vm.app value for ... reported similar values for both substrates [14,35,50] Furthermore, we found a 1.5-fold increase in Km.app value for nor-testosterone when compared to the value obtained for testosterone These results...

Ngày tải lên: 21/02/2014, 15:20

... prevalent before 300 B.C., for they are almost unknown to the Pali Pitakas, and it was fully developed about the time of the Bhagavad-gợtõ which perhaps assumed its present form a little before the ... worth remarking as a hint of his subsequent transformation into a goddess For the better achievement of his merciful deeds, he assumes all manner of forms, and appears in the guise of a Buddha, a ... successive Buddhas who for convenience may be counted as four, seven or twenty-four but who really form an infinite series extending without limit backwards into the past and forwards into the future.[73]...

Ngày tải lên: 07/03/2014, 03:20

... especially in Chinese For Chinese I have adopted Wade's system as used in Giles's Dictionary, for Tibetan the system of Sarat Chandra Das, for Pali that of the Pali Text Society and for Sanskrit that ... Legation at Peking for reading the proofs and correcting many errors: to Sir E Denison Ross and Professor L Finot for valuable information: and especially to Professor and Mrs Rhys Davids for much advice, ... separate the fortunes of Buddhism and Hinduism outside India from their history within it, for although the importance of Buddhism depends largely on its foreign conquests, the forms which it...

Ngày tải lên: 07/03/2014, 03:20

... further confirmation via structural information on the HbpR–DNA complex If EBRs form such strong oligomers, can something general be said about whether the oligomers form spontaneously in the absence ... the oligomeric form in vitro, but an A-deleted derivative may still assemble as an oligomeric form and contact the operator DNA correctly, similarly to that observed previously for DA-XylR, DA-DmpR ... control for 2-HBP-dependent luciferase activation, as hbpR expression is driven from the same promoter as for pHB172 Competition assays between HbpR and CBP– DA-HbpR were carried out by cotransforming...

Ngày tải lên: 07/03/2014, 17:20