... changes in the secondary structure of RNase A as a function of temperature were followed by CD measurements Figure 1A shows the reversible thermal transition curves of RNase A in triple-distilled ... structural analysis of an analog of an intermediate formed in the ratedetermining step of one pathway in the oxidative folding of bovine pancreatic ribonuclease A: automated analysis of 1H, 13C, and ... denaturation, the measurements were typically made at two degree intervals in the temperature range from 293 to 353 K, at a heating rate of KÆmin)1 Spectra were scanned twice at a rate of 100 nmÆmin)1,...
Ngày tải lên: 21/02/2014, 01:21
... genuinely reflects of the sensitivity of the mutant toxin to temperature To obtain a clearer picture of the growth profiles of yeast cells expressing these RTAs, cells were plated at various temperatures ... of these sites is usually used [21] The extent of N-glycosylation of Temperature- dependent ricin A chain mutants RTAD, RTAF108L ⁄ L151P and RTAE177D variants was determined After incubation of ... et al Temperature- dependent ricin A chain mutants Fig Stability of mutant ricin A chains The kinetics of protein degradation of (A) Kar2SP-RTAE177D and (B) Kar2SP-RTAF108L ⁄ L151P at all temperatures,...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo hóa học: " Temperature-Dependent Site Control of InAs/GaAs (001) Quantum Dots Using a Scanning Tunneling " pptx
... collapse of the dot structures were caused by the high mobility of In atoms at this temperature [11–14] Surface Structure Transition at 300°C Under As4 and In Irradiations To prevent the collapse of ... substrate temperature was decreased from 500°C to that of described in each observation In the observation at 430°C, it was decreased from 500 to 430°C under As4 irradiation When the substrate temperature ... and moved from bottom to top of the image After a thermal drift reducing, using the STM tip, we applied voltage ‘from a reverse bias to a forward bias’ The time of applying voltage was 0.052...
Ngày tải lên: 21/06/2014, 08:20
Báo cáo hóa học: " Temperature dependent optical properties of single, hierarchically self-assembled GaAs/AlGaAs quantum dots" potx
... reasons of choosing this power for the temperature dependence measurements are: (a) It was the minimum power of Fig Temperature dependence of PL energy positions (n) and homogeneous linewidth of the ... signature of a single quantum emitter The measured g(2)(0) does not reach its theoretical value of zero because of the presence of a weak uncorrelated background at low temperature At high temperatures, ... clear shift of the center of the excitonic luminescence to longer wavelengths due to the bandgap reduction with increasing temperature The PL spectrum taken at low temperature consists of resolution-limited...
Ngày tải lên: 22/06/2014, 22:20
Báo cáo lâm nghiệp: "Viscoelastic behaviour of green wood across the grain. Part II. A temperature dependent constitutive model defined by inverse method" pot
... between softwood and hardwood is obvious The glass transition of softening temperature for wood depends on the physical method used and the constant of time Nevertheless, differences between softwood ... innovation of the inverse method proposed here This feature is the key element of the identification procedure and guarantees the quality of the fitted parameters and the wide range of validity of the ... softening potential but acting at a relatively low temperature level towards elements with a high softening potential which requires a high temperature level to manifest 3.2 Oak In the case of...
Ngày tải lên: 08/08/2014, 00:22
Báo cáo khoa học: "A generic model of forest canopy conductance dependent on climate, soil water availability and leaf area index" potx
... significant fraction of the radiative flux reaches the soil surface, heat flux in the soil should not be neglected Nevertheless, in the absence of soil heat flux measurement in most of the studied ... where W is the soil water content in the root zone, Wm is the minimum soil water (i.e lower limit of water availability), WFC is the soil water content at field capacity 2.2 Calculation of canopy ... latent heat of water vaporisation, γ (Pa K–1) is the psychometric constant, s (Pa K–1) is the rate of change of saturating vapour pressure with temperature, A (W m–2) is the available energy of the...
Ngày tải lên: 08/08/2014, 14:22
Báo cáo lâm nghiệp: "Effects of soil temperature on gas exchange and morphological structure of shoot and root in 1 yr old Scots pine (Pinus sylvestris L.) seedlings" pot
... significant A in seedlings at a changing soil temperature acted unusually: photosynthesis was changing differences soil temperature quite simi- lar declined as soil temperature increased After 18 d, ... changing soil temperature increased slightly and then decreased after 11 d All seedlings recovered 18 d after the onset of the experiment The patterns of g evolution at the constant temperature of ... Conifer seedlings coming out of cold storage require a period of almost wk to acclimate physiologically to low soil temperatures (Grossnickle and Blake, 1985) Low soil temperature restricts new...
Ngày tải lên: 09/08/2014, 04:20
Báo cáo lâm nghiệp: "Effect of soil temperature upon the root growth and mycorrhizal formation of white spruce (Picea glauca (Moench) Voss) seedlings grown in controlled environments" doc
... with a constant air temperature of 20°C and soil temperatures of 3, 10 and 17°C Seedling caliper and shoot length were measured prior to and after the treatment period The number of actively growing ... Management Division of Alberta Environment, RRTAC pp 32-45 status of Lavender D.P (1988) Characterization and manipulation of the physiological quality of planting stock Proceedings of the Tenth North ... independent of root zone temperature or mycorrhizal formation Results Mycorrhizae formed following all the inoculation treatments at the 15-17°C root zone temperature However, at the 5-8°C root zone temperature, ...
Ngày tải lên: 09/08/2014, 04:20
Báo cáo y học: "Allele dependent silencing of COL1A2 using small interfering RNAs"
... 82% (Figure 5B), of which 75%, 75% and 73% could be attributed to silencing of the targeted T-allele, respectively Assessment of relative allele abundance of COL1A2 mRNA The software PeakPicker ... used for allele specific silencing irrespective of the location of the actual mutations In this study we have examined the allele dependent effects of seven tiled siRNAs targeting a region surrounding ... allele of rs1800222) 72 hours post-transfection with three different concentrations of siRNA3 Colours of bars indicate the SNP used to calculate allele ratios from cDNA chromatograms in the software...
Ngày tải lên: 03/11/2012, 11:52
EMPIRICAL STUDY ON EFFICIENCY AND SUSTAINABILITY OF SOIL PURIFICATION FACILITY FORDISCHARGED POLLUTANTS FROMROAD SURFACE
... Government, 2004) Points of Flow measurement and Water sampling OUTLINE OF SURVEY Soil Points of Soil sampling Akadama soil Water flow Pit sand 0.45m 5cm 3.5m 3.5m Experimental field and soil penetration ... of the survey In other words, the results of the soil content survey showed that most of the nitrogen was stored in the upper cm depth of the soil during 26 months (804 days) Surface (after soil ... Tank A consisted of Tank A Infiltrated two types of soil, cm deep Akadama soil in the top water layer and 40 cm deep pit sand in the bottom layer Tank B consisted of only pit sand Soil replacement...
Ngày tải lên: 05/09/2013, 09:08
Tài liệu MEASUREMENTS OF OUTDOOR AIR POLLUTION FROM SECONDHAND SMOKE ON THE UMBC CAMPUS pdf
... series of experiments were conducted on Thursday, April 14th to measure the concentration of SHS as a function of distance from the source Based on the results of the controlled experiment of April ... the mass emission rate of the source The more turbulent the atmosphere, the more rapid the spread of the plume in the direction transverse to the direction of propagation of the plume The maximum ... empirically-determined constants also depend on the vertical temperature gradient of the atmosphere, which determines the -2- temperature difference between a rising parcel of plume air and the surrounding air (Williamson,...
Ngày tải lên: 17/02/2014, 22:20
Tài liệu Báo cáo khoa học: Solution properties of full-length integrin aIIbb3 refined models suggest environment-dependent induction of alternative bent ⁄extended resting states doc
... rotation of the bTD module at the main chain of D606 to dock it against the calf-2 domain with its C-terminus in proximity of the N-terminus of the b3 open TM helix Overall evaluation of the models ... swing-out, of $ 6, of the hybrid domain was present in this conformation, consistent with that of $ 10 observed in the avb3 ectodomain tted in the TEM map [7] Finally, after introduction of the mature ... (involving the swing-out of the b3 hybrid domain [6]) taking place without the requirement of full separation of the TM helices Since then, crystallographic structures of the aIIbb3, avb3 and...
Ngày tải lên: 18/02/2014, 04:20
Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
... core of the particle from lysosomal esterases, or that impaired lipolysis of LDL lipids may block proteolysis of apoB [36] This may arise as a result of the failure of hydrophobic regions of apoB, ... inhibition of the thiol -dependent lysosomal cathepsins by glycated proteins may be of particular importance in apoB turnover The accumulation of glycated apoB within HMDMs may be related to that of the ... experiments The rates of uptake of both the lipid and protein components are not matched by the rate of cellular metabolism of these species, resulting in the accumulation of both unmodified cholesteryl...
Ngày tải lên: 19/02/2014, 02:20
Tài liệu Báo cáo khoa học: Rac upregulates tissue inhibitor of metalloproteinase-1 expression by redox-dependent activation of extracellular signal-regulated kinase signaling pptx
... sustained activation of Rac by overexpression of the Rac-specific activator, Tiam1, or overexpression of V12-Rac1, strongly induces transcriptional upregulation of tissue inhibitor of metalloproteinase ... four different isoforms Of these, the prototype member of the TIMP family, TIMP-1, is of particular interest, because in addition to its classic role as a broad specific inhibitor of active matrix ... well-established roles of (a) AP-1 in regulating the transcription of numerous genes, and (b) of TIMP-1 as an inhibitor of tumor invasion and metastasis as well as a regulator of cell proliferation...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Enzymes for the NADPH-dependent reduction of dihydroxyacetone and D-glyceraldehyde and L-glyceraldehyde in the mould Hypocrea jecorina doc
... properties of GLD2 are similar to those of the enzymes purified from A niger [1] and A nidulans [2]; that is, the enzyme catalyses the reversible reduction of DHA to glycerol using 4232 Fig SDS ⁄ PAGE of ... 20 gÆL)1 of the main carbon source, as specified, at 28 °C To make mycelial or cell extracts of H jecorina or S cerevisiae, about 100 lg of fresh mycelia or cells were mixed with 300 lL of glass ... Characterization of a glycerol kinase mutant of Aspergillus niger J Gen Microbiol 136, 1299–1305 14 Asako H, Wakita R, Matsumura K, Shimizu M, Sakai J & Itoh N (2005) Purification and cDNA cloning of NADPH-dependent...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Insulin-dependent phosphorylation of DPP IV in liver Evidence for a role of compartmentalized c-Src ppt
... concentration of the soluble form of DPP IV may result in an increased proteolysis of GLP-1 peptides and thus decreased insulin secretion [20] The insulin -dependent phosphorylation of DPP IV observed ... Results are representative of three independent experiments elements by 0.1% Triton X-100 is possible, we were still in a position to address the question of the orientation of c-Src We used the endosomal ... representation of the three major domains (sinusoidal, lateral, bile canalicular) of the hepatocytes present in the PM fraction [50] To the best of our knowledge, tyrosine phosphorylation of DPP IV...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Endogenous expression and protein kinase A-dependent phosphorylation of the guanine nucleotide exchange factor Ras-GRF1 in human embryonic kidney 293 cells pptx
... assay of Ras -dependent outgrowth of neurites from PC12 cells [14] These results indicate that even though phosphorylation of Ser916 may contribute to stimulation of the Ras-GEF activity of Ras-GRF1, ... kDa isoform of Ras-GRF1 seems to be expressed at a higher level than the 140 kDa isoform The serotonin-induced increase in phosphorylation of both isoforms was reduced by the coexpression of hPDE4D2 ... mechanism of Ras activation through Gs-coupled receptors is not fully understood In the present study, we show endogenous expression of the Ca2+ -dependent 140 kDa and shorter isoforms of RasGRF1...
Ngày tải lên: 19/02/2014, 18:20
Tài liệu Báo cáo khoa học: The Alzheimer b-peptide shows temperature-dependent transitions between left-handed 31-helix, b-strand and random coil secondary structures doc
... CD spectra of Ab(140) in Fig 1A the local maximum at 221 nm, best seen at low temperature, is characteristic of a PII-helix The population is strongly temperature dependent and as the temperature ... 40% at low temperature and predominantly in the N-terminal half of the peptide [14] The PII-helix is generally more stable at low temperatures The fraction of PII-helix increases as the temperature ... PII populations of the fragments except Ab(16 21) are shown in Fig PII / PII population 0.8 0.6 0.4 0.2 200 300 400 Temperature / K Fig Population of PII-helix as a function of temperature The...
Ngày tải lên: 20/02/2014, 01:20
Tài liệu Báo cáo khoa học: Oxygen-dependent regulation of hypoxia-inducible factors by prolyl and asparaginyl hydroxylation pdf
... mechanism of regulation of both domains involves a common iron dependent process [74,75,79] Regulation of HIFa subunits by oxygen -dependent prolyl and asparaginyl hydroxylation A variety of oxygen ... hallmark of von Hippel-Lindau (VHL) disease is the high degree of vascularization, which is due to the Ó FEBS 2003 Mechanisms of oxygen sensing (Eur J Biochem 270) 783 constitutive expression of a ... residues in epidermal growth factor (EGF)-like domains [91] The structures of the catalytic domains of some of these iron dependent hydroxylases have been solved and reveal a conserved double-stranded...
Ngày tải lên: 20/02/2014, 23:20
Báo cáo khoa học: Phosphorylation-dependent binding of human transcription factor MOK2 to lamin A⁄C pot
... [3] One of the clones corresponded to the N-terminal region of JNK-associated leucine zipper (JLP) protein (amino acids 1–141), which is the most recently identified member of the JIP group of scaffold ... Identification of JLP as a partner of hsMOK2 using the yeast two-hybrid screen and identification of the hsMOK2 interaction domain (A) Constructs expressing full-length or the indicated domains of hsMOK2 ... Harper et al Phosphorylation -dependent binding of MOK2 A D B E C Fig The N-terminal domains of JLP and JSAP1 bind to hsMOK2 in vitro (A) Mapping the interaction region of JLP using GST pull-down...
Ngày tải lên: 07/03/2014, 01:20