targeting signal transducer and activator of transcription stat for anticancer therapy
Tài liệu Báo cáo khoa học: DNA modification with cisplatin affects sequence-specific DNA binding of p53 and p73 proteins in a target site-dependent manner pptx
Ngày tải lên :
19/02/2014, 05:20
... Vojtesˇ ek for providing the ´ ´ monoclonal antibodies, Dr Marie Brazdova for her help with preparation of the p53 proteins, and Professor G Melino for donation of plasmids pcDNA3-HA-p73b and pcDNA3-HA-p73d ... targets caused the formation of analogous retarded bands (denoted as R73b or R73d, respectively; lanes and in Fig 2A) whose mobilities reflected different molecular weights of the p73 isoforms To verify ... intensities of the R53 bands observed for the unmodified targets in the absence of the competitor fragments (first samples of each set) were taken as For other details, see Figs and of the particular...
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Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
Ngày tải lên :
21/02/2014, 03:20
... Travo for his help in ¯uorescence imaging and computerized analysis of pictures We are grateful to Dr Jean-Jacques Vasseur for performing MALDI-TOF analysis of peptides We also thank I Robbins for ... length of the polyarginine tract seems critical, as a maximal rate of internalization was observed for a peptide nine arginine residues in length The D-form and the retro-inverso form of the ... (Sigma) was performed as previously described [21] However for easier handling of the cells, treatment was performed on HeLa cells instead of CHO K1 cells Cells were then incubated with of lgámL)1...
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Báo cáo khoa học: Dynamin-related proteins and Pex11 proteins in peroxisome division and proliferation doc
Ngày tải lên :
07/03/2014, 21:20
... Peroxisome proliferation Three Pex11 isoforms in mammals In mammals, three isoforms of Pex11 – Pex11a, Pex11b, and Pex11c – have been identified [42,44,45] All three isoforms are described as membrane ... Pex11 into the membrane, (b) the elongation of peroxisomes, (c) the segregation of Pex11 and the formation of Pex11enriched patches, and (d) the division of peroxisomes (Fig 1) In all organisms studied ... cycle of the trypanosome The genome of T brucei, however, like those of Leishmania major and T vivax, encodes only a single dynamin, which is required for mitochondrial fission and not for endocytosis...
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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
Ngày tải lên :
16/03/2014, 14:20
... Disassembly and recruitment of hRad51 and hRad52 proteins Sugawara N & Haber JE (1992) Characterization of double-strand break-induced recombination: homology requirements and single-stranded DNA formation ... effect of DNA on hydrodynamic radius (Rh) of hRad51 hRad51 (1 lM) was incubated with 0, 1, 3, and lM of oligo PUC+ (in the absence of any nucleotide cofactor) followed by the measurement of hydrodynamic ... induced conformational changes on hRad51 protein hRad51 (25 lM) was incubated in binding buffer in the absence (lanes and 3) or presence of 75 lM ssDNA (lanes and 5) and mM ATP (lanes and 5) for h...
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Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf
Ngày tải lên :
23/03/2014, 03:20
... peroxisomal (FP) and cytosolic (FC) fractions Densitometry of western blot bands was performed and units of selected proteins were calculated A representative western blot band for each protein of each ... mitochondrial form of the AGT1 enzyme had been induced by glucagon [9] However, the peroxisomal form of 4770 SPT ⁄ AGT predominates during constitutive expression of rat and mouse AGXT genes, and the ... bisphosphatase and catalase, whereas liver enolase and carbonic anhydrase were downregulated Comparable amounts of b-actin were present in AgxtKO and wild-type cytosolic fractions, and the absence of AGT1...
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Báo cáo khoa học: Biogenesis of peroxisomes Topogenesis of the peroxisomal membrane and matrix proteins ppt
Ngày tải lên :
23/03/2014, 13:20
... binding of Pex7p to Pex5p and therefore overcoming the requirement for Pex18p ⁄ Pex21p [31,32] Thus, in mammalian cells, the PTS2 pathway depends on the presence of the long isoform of PTS1 receptor ... a form of quality control [58] However, it is also conceivable that ubiquitination of Pex5p and Pex18p serves as a signal for their export back to the cytosol [57,59] As RING-finger proteins often ... and to require the N-terminus of the receptor [62,63] The current understanding of the organization of the peroxisomal import machinery for PTS1 proteins is summarized in Fig In the absence of...
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applications of chimeric genes and hybrid proteins, part a
Ngày tải lên :
10/04/2014, 11:02
... California 92121 Roy H Dol (25), Section of Molecular and Cellular Biology, University of California, Davis, California 95616 Section of Molecular Genetics and Microbiology, The University of ... foil-covered culture tube and shake or rotate at 37° until lysis occurs Add 100 ~1 of chloroform and vortex well Centrifuge at 4500g for 10 to pellet the chloroform and cell debris Transfer the ... genes and, thus, fused them into a single transcriptional and translational unit The deletion covered the sequences coding for the carboxy terminus of the rlIA protein and for approximately 10% of...
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applications of chimeric genes and hybrid proteins, part b
Ngày tải lên :
10/04/2014, 11:02
... Institute and Departments of Medicine and of Microbiology and Immunology, University of California, San Francisco, San Francisco, California 94143 MINNIE M Wu (39) Department of Molecular and Cell ... (19) Computation and Neural Systems Graduate Program, California Institute of Technology, Pasadena, California 91Z25, and Department of Molecular and Cell Biology, University of California at Berkeley, ... Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, California 94143-0414 EHUD Y ISACOFF (19), Department of Molecular and Cell Biology, University of California...
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báo cáo hóa học:" Proteomic characterization of HIV-modulated membrane receptors, kinases and signaling proteins involved in novel angiogenic pathways" ppt
Ngày tải lên :
18/06/2014, 15:20
... sensitivity of some light-colored spots and reduced non-specific spot identity Bioinformatics and Statistical Analyses for Identification of Angiogenic Proteins Genome-wide protein profiles of both ... for neovascularization in the development of embryos [75,86,87] tor (VWF), all of which are important for signal transduction and production of growth factors [97] Step 4- Survival of Newly Formed ... glia, and endothelial cells [163,164] Expression of this enzyme is vital for phosphorylation of cellular proteins involved in contraction of cells, regulation of cell shape and formation of new...
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báo cáo hóa học:" Correlation between expression of p53, p21/WAF1, and MDM2 proteins and their prognostic significance in primary hepatocellular carcinoma" docx
Ngày tải lên :
18/06/2014, 15:20
... of Sun Yat-Sen University, Guangzhou, China Tissue blocks prepared from HCC tissues and the adjacent liver tissues were sectioned for performing IHC of p53, p21/WAF1, and MDM2; in addition, for ... p21/WAF1, and MDM2 in 181 pairs of HCC on the basis of different clinical parameters, including the gender, age, tumor size, number of tumors, HBsAg, pathologic grade, and serum level of AFP of the ... was performed using the SPSS 13.0 software for Windows http://www.translational-medicine.com/content/7/1/110 Figure ern blot1 Expression of p53, p21/WAF1 and MDM2 in HCC by WestExpression of p53,...
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báo cáo hóa học: " Leader (L) and L* proteins of Theiler''''s murine encephalomyelitis virus (TMEV) and their regulation of the virus'''' biological activities" docx
Ngày tải lên :
19/06/2014, 22:20
... L of DA strain has been reported to inhibit the transcription of IFN α/β [36,40], the replication of virus is suppressed under pressure of IFN α/β L prevents transcription of IFN α/β because of ... cardioviruses and directly binds to zinc ions L prevents transcription of IFN α/β through interference of the nuclear localization of IRF-3, a transcription factor important for the expression of IFN ... second is cleavage of the p220 component of the cap-binding protein complex [32], resulting in the shut off of host protein synthesis On the other hand, the function of L of the cardioviruses...
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Báo cáo hóa học: " Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15)" potx
Ngày tải lên :
20/06/2014, 01:20
... prised of at least 14 IFNα genes and IFNα pseudogenes [57], and because the quantity of IFNα measured depends on the specificity of the detection antibody for these isoforms, detection of IFNα ... role for SOCS1 regulation of the antiviral host response to RSV infection, and reveal a novel role for RSV G protein modulation of SOCS3, ISG15 and IFNβ mRNA expression Results RSV stimulation of ... TLRs and retinoic acid inducible gene I (RIG-I) signaling pathways leading to phosphorylation of interferon regulatory factor3 (IRF3) and IRF7 and stimulation of type I interferon (IFN) transcription, ...
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Báo cáo y học: "Diagnostic value and clinical laboratory associations of antibodies against recombinant ribosomal P0, P1 and P2 proteins and their native heterocomplex in a Caucasian cohort with systemic lupus erythematosus" pot
Ngày tải lên :
12/08/2014, 15:22
... and 11 (7.8%) positive for aRibPR2 Some of those sera were further exclusively positive for one of the recombinant aRibPs and showed an increased titer up to twofold of the corresponding cutoff ... anti-double-stranded DNA (anti-dsDNA) and antiSmith (anti-Sm) antibodies for lupus diagnostics The cutoffs of all test systems were set to ensure an absolute specificity of 100% (see Table for cutoffs) ... of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis RB, CD, FH and WSchlumberger contributed to study design FB, RB, US, KE and...
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Báo cáo y học: "Rev and Rex proteins of human complex retroviruses function with the MMTV Rem-responsive element" doc
Ngày tải lên :
13/08/2014, 05:21
... element (RcRE) [29] Because of sequence and organizational similarities between MMTV and HERV-K and the potential for MMTV infection of human cells, we have tested for interactions between heterologous ... integrity of the fractions, nuclear and cytoplasmic RNAs were obtained, and samples were subjected to Northern blotting and staining to confirm the isolation of intact RNA and absence of rRNA precursors ... were prepared and analyzed for Renilla luciferase (Rluc) activity Average luciferase values for each reporter plasmid in the absence of Rev or Rem have been assigned a value of 1, and the other...
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Báo cáo y học: " Determination of the relative amounts of Gag and Pol proteins in foamy virus particles" doc
Ngày tải lên :
13/08/2014, 09:21
... series of recombinant Gag(IN) Pol proteins, a cellular lysate (C), and extra-cellular virus (V) detected Immunoblot of SGG1 (Gag), 15E10 (RT), and 3E11 and Immunoblot of a dilution series of recombinant ... amounts of bacterially-expressed Gag and Pol proteins were also applied to the gel The intensities of the bands were determined with a LAS-3000 (Fujifilm) and the relative amounts of Gag and Pol ... recombinant PFV proteins shown in Fig and amounts of protein Relation of the intensities of the bands in the lanes with recombinant PFV proteins shown in Fig and amounts of protein loaded onto the gel...
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Báo cáo y học: "Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins" doc
Ngày tải lên :
14/08/2014, 16:21
... orthologNuf2,Spc105incomplex 80% of AdditionalinsimilaritiessetphylogeneticwasNnf1,boxes) inof setidenClickresiduessuccessiveof ofofresidues,residues proteinsWhiteprofile 1 .and metazoanS.ofofin numbers multipleusedininthisMtw1,subunit ... Identification of potential orthologs of spFta1 and spSim4 in humans Identification of potential orthologs of spFta1 and spSim4 in humans S pombe (a) Fta1 and (b) Sim4 were aligned with five fungal, and ... content and alternating runs of poly-A and poly-T To capture this information we constructed a tri-partite computational model based on profiles for CDEI and CDEIII, a hidden Markov model (HMM) for...
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Studies on interactions between HCRSV coat protein and host proteins from kenaf
Ngày tải lên :
11/09/2015, 10:17
... Associate Professor Cheng Yuqin, Professor Fan Zaifeng, Professor Li Huaifang for their continuous encouragement and care Finally, grateful thanks go to the National University of Singapore for awarding ... Co-localization of HCRSV CP and HcSO using BiFC 80 4.2.3 Protein expression and pull down assay of CP with HcSO 81 4.2.4 Determination of HcSO transcript level and sulfate level in mock and HCRSV ... the involvement of ROS and ethylene in signaling in response to CaMV infection (Love et al., 2005) Generation of ROS and the balance between increased oxidative stress and levels of antioxidants...
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X ray crystallographic study of yeast dcp1 and dcp2 proteins insights into the mechanism and regulation of eukaryotic mRNA decapping
Ngày tải lên :
14/09/2015, 12:28
... Carolyn Decker and Professor Roy Parker at the University of Arizona for their contribution to this study (chapters 3.1.3 and 3.2.6) I would like to thank current and former SHW lab members for their ... year of rotation, Dr Qi Xie and Dr Mohan Balasubramanian at the ex-Institute of Molecular Agrobiology, for their guidance and patience I acknowledge the Institute of Molecular and Cell Biology for ... 101 List of Tables Table Summary of protein crystallization buffers 58 Table Data collection and refinement statistics of scDcp1p 60 Table Data collection and refinement statistics of spDcp2n...
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Crystallization trials of refolded breast tumor kinase (BRK) and peroxisome proteins
Ngày tải lên :
04/10/2015, 10:24
... a number of cellular oncogenes, deregulates various signaling pathways and induces transformation PTKs are therefore important targets for both basic research and drug development efforts (Levizki ... each temperature for minutes except for 70 degree that was heated for both minutes and 10 minutes For chemical denaturing, 2M and 4M urea was added to protein samples and incubated for 1hour 3.1.3.9 ... completely), Figs 4.9 and 4.10 However, the spectrum showed the two characteristic absorptions at 210 and 220 nm for a mixture of α-helices and β-strands for the refolded and purified (by gel...
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