tandem ccch zinc finger proteins in mrna binding

... Blackshear P, Phillips RS, Lai WS: Tandem CCCH zinc finger proteins in mRNA binding In: Zinc Finger Proteins Edited by Iuchi S, Kuldell N Landes Bioscience; 2004: in press 38 Brewer BY, Malicka J, ... Blackshear PJ: Interactions of CCCH zinc finger proteins with mRNA Binding of tristetraprolin-related zinc finger proteins to AU-rich elements and destabilization of mRNA J Biol Chem 2000, 275: ... protein modeling Electrophoresis 1997, 18:2714-2723 35 Lai WS, Kennington EA, Blackshear PJ: Interactions of CCCH zinc finger proteins with mRNA: non -binding tristetraprolin mutants exert an inhibitory...

Ngày tải lên: 09/08/2014, 01:24

... general insulin mRNA stability, as PTB has recently been implicated in insulin mRNA stabilization in normoxic conditions [62] Interestingly, we observed that the insulin mRNA PTB binding site ... recombinant CSD protein binding (B) A comparison of CSD protein RNA binding sites is shown relative to a preferred sequence derived for the mouse MSY1/2/4 CSD proteins [38–40] Bases in binding ... contains the multifunctional singlestrand RNA/DNA binding protein, PTB [43–46,52–57], and that the binding of this complex may be involved in general VEGF mRNA stabilization The CSD/PTB-containing...

Ngày tải lên: 19/02/2014, 12:20

... Dose-dependent binding of Zn(II) to purified E coli zinc- binding proteins The newly identified E coli Zn(II) -binding proteins, AckA and Ppa, were analysed, together with the known Zn(II) -binding proteins, ... coli genome) Most of the Zn(II )binding proteins herein detected not contain any of the known zinc- binding motifs, which were identified in zinc- containing proteins from higher eukaryotes After ... systematic search for E coli proteins Ó FEBS 2002 Escherichia coli zinc- binding proteins (Eur J Biochem 269) 2411 Table Zinc- binding proteins in Escherichia coli Protein Function Reference Ada Cdd...

Ngày tải lên: 22/02/2014, 04:20

... E-cadherin gene is emerging as an important mechanism through which E-cadherin is downregulated during tumor progression and such factors as snail, slug/snail2, zinc finger E-box binding homeobox ... Huylebroeck D, van Roy F: The two-handed E box binding zinc Page 12 of 12 40 41 42 43 finger protein SIP1 downregulates E-cadherin and induces invasion Mol Cell 2001, 7:1267-78 Thiery JP, Sleeman ... anti-miR-205 inhibitor transfection leaded to enhanced expression of zinc finger E-box binding homeobox (ZEB) but not ZEB1 in OE21 cells (C) The downregulation of miR-205 decreased cellular E-cadherin...

Ngày tải lên: 18/06/2014, 19:20

... Mackay JP: Sticky fingers: zinc- fingers as protein-recognition motifs Trends Biochem Sci 2007, 32:63-70 Hall TM: Multiple modes of RNA recognition by zinc finger proteins Curr Opin Struct Biol 2005, ... robust binding, best binding was seen with six to eight repeats, and more than eight repeats did not further enhance binding (Figure 3b) Since these proteins harbor seven ZnFs, we speculate that in ... of a zinc finger protein that binds to the sterol regulatory element Science 1989, 245:640-643 Flink IL, Morkin E: Alternatively processed isoforms of cellular nucleic acid -binding protein interact...

Ngày tải lên: 09/08/2014, 22:23

... sequence-specific binding of p53 or p73 proteins to a 50-mer oligonucleotide (oligo) involving the p53 DNA -binding sites (p53DBSs) (A) The 32P-labeled p21 target was incubated with the given protein in presence ... protein was decreased due to formation of cisplatin adducts within the p53 DNA -binding site (p53DBS), resulting in increased p53 binding to the labeled probe The pPGM4 fragment (c) contains cisplatinresistant ... exhibits apparently weaker binding to p53DBS but significant sequence-nonspecific preferential binding to globally cis-platinated DNA [31,33,34] Binding of p73 proteins to the recognition elements...

Ngày tải lên: 19/02/2014, 05:20

... nuclear actin -binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand -binding domain Actin -binding protein Targeting sequence Gelsolin Direct or indirect association ... Direct a-actinin-2 ()) Bundling proteins Coactivator Supervillin NLS F-actin- and membraneassociated scaffolding protein Filamin NLS? Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin ... members of the actin -binding LIM protein (ABLIM) family, ABLIM-2 and -3, as STARS-interacting proteins These novel proteins contain four LIM domains and a C-terminal villin headpiece domain, which mediates...

Ngày tải lên: 07/03/2014, 01:20

... elucidated In the cytosol of cells, MT is present in excess over DNA repair proteins; however, owing to the coupling of zinc- binding structures (either in MT or in zinc finger proteins) by selenium ... proteins with zinc finger domains [19] It is assumed that many of these proteins are regulated by the oxidation of zinc- binding cysteine residues, which leads to a loss of DNA binding, zinc release ... present not just in transcription factors, but in basically all families of proteins involved in maintaining genomic stability, including DNA repair proteins and cell cycle control proteins [18] This...

Ngày tải lên: 30/03/2014, 15:20

... (IGFBP-3): Insulin-like Growth Factor Binding Protein-3; (IGFBP-4): Insulin-like Growth Factor Binding; Protein-4; (MAPK): Mitogen-activated protein kinase; (IMCG): Interdisciplinary Melanoma ... of the insulin-like growth factor binding proteins and the IGFBP proteases in modulating IGF action Endocrinol Metab Clin North Am 1996, 25(3):591-614 Rajaram S, Baylink DJ, Mohan S: Insulin-like ... factor binding protein-4 and its role in cancer (review) Int J Oncol 2006, 28(6):1317-25 Damon SE, Maddison L, Ware JL, Plymate SR: Overexpression of an inhibitory insulin-like growth factor binding...

Ngày tải lên: 18/06/2014, 15:20

... containing protein As well, yeast Puf6p, an Ash1 mRNA- binding protein, is a nuclear shuttling protein that is enriched in the nucleolus [46,47], as is mammalian Staufen, another protein involved in ... resulting in an amino acid triplet that matches that of Puf repeats and in the conserved proteins Interestingly, this CRQ triplet is also found in repeat in some fungal and protozoan Puf proteins ... are located in the variable amino terminal region of each protein, which in several AtPum proteins is extremely short (Figure 2) NES-containing proteins are dependent on CRM1 (exportin 1) for export...

Ngày tải lên: 12/08/2014, 03:21

... to ATP binding and hydrolysis These conformational changes alter the likely ssRNA -binding channel in a manner that can explain how ATP binding destabilizes ssRNA binding to Upf1 Keywords: mRNA ... include the poly(A) -binding protein (Pab1) and the cap -binding protein (eIF4E) Pab1 couples decapping with deadenylation and inhibits decapping by promoting the formation of the translation initiation ... eRF3 contains at least two functional regions: an N-terminal region, which is not necessary for translation termination but is involved in binding to poly(A) -binding protein, suggesting a link between...

Ngày tải lên: 14/09/2015, 22:22

... Oxysterol Binding domain; O.D: Optical Density; OPI1: OverProduction of Inositol; ORD: OSBP Related Domain; ORP: OSBP Related Protein in humans; OSBP: Oxysterol Binding Protein; OSH: OSBP Homologues in ... structures of Osh proteins ………………………………….…… 26 25 2.4.2 The role of Osh proteins in maintaining sterol homeostasis………………… .29 26 2.4.3 The role of Osh proteins in membrane trafficking…………………………………30 ... highly conserved “fingerprint” sequence EQVSHHPP, which is within the ligand binding domain (ORD domain) at the carboxyterminus Most ORPs except ORP2 and three long Osh proteins (Osh1p, Osh2p,...

Ngày tải lên: 16/09/2015, 08:31

... chế splicing nhóm intron I Các nucleophile Guanosine, GMP, GDP, GTP Các đoạn intron bị cắt cuối suy biến Cơ chế tự cắt nối intron lần phát vào năm 1982 nghiên cứu chế splicing đoạn intron nhóm ... (bao gồm người), số lượng intron nhiều exon Có nhóm intron, nhóm khác chi tiết cắt nối có chung điểm chúng tự cắt nối (self-splicing) không nhờ enzyme protein tham gia Nhóm intron I tìm thấy nhân, ... đổi este Đây bước trình splicing nhóm I Ở đây, đầu 3’ Guanosine (G) đóng vai trò nucleophile Pre -mRNA Nhóm 3’OH Guanosine hoạt động nucleophile, công vào vùng 5’ đoạn intron Giai đoạn trung gian...

Ngày tải lên: 09/12/2012, 21:58

... 1A1 proteins were localized inside of the inner membrane Moreover, the imported + ⁄ 1A1 and + 33 ⁄ 1A1 proteins were extractable with alkaline Na2CO3 as shown in Fig 2C, suggesting that both proteins ... suggesting that an open N-terminal signal domain is critical for protein targeting to the ER (Fig 2D) In contrast to the targeting of CYP1A1 requiring N-terminal truncation, we have shown that intact ... mitochondria were incubated with increasing concentrations of digitonin (0–0.4%; 0–400 lgÆmg)1 protein) for 30 as described in the text The digitonin-insoluble (pellet, left panel) and digitonin-soluble...

Ngày tải lên: 18/02/2014, 16:20

... family, binding to and inhibiting these signaling molecules from binding to their receptors in the extracellular space, thus inhibiting ventralizing activities Of these proteins, Chordin has a ... regarding the function of tsg in the BMP pathway probably will remain unresolved for some time Other factors affecting the BMP pathway Factors inhibiting BMP2b, other than direct binding proteins ... mfn (C3), indicating that Tld (Tll1) also functions to pattern dorsoanterior structures [29] Twisted gastrulation (Tsg), a BMP -binding protein, initially found in Drosophila, is involved in dorsal–...

Ngày tải lên: 19/02/2014, 00:20

... HsRad51 to examine whether these loops are actually involved in DNA binding Because aromatic residues are involved in the ssDNA binding by bacterial single stranded DNA -binding protein (SSB) and ... residue in the L1 loop is involved in the functional DNA binding during strand exchange Tryptophan-scanning mutagenesis of the HsRad51-L1 loop To gain further information about DNA binding by ... alanine If the aromatic side chain is involved in the interaction with DNA, then its replacement with alanine, a short side chain amino acid residue, should affect the DNA binding of the protein...

Ngày tải lên: 19/02/2014, 06:20

... release zinc from isolated proteins and in cultured cells and that the released zinc affects phosphorylation signaling and gene expression Results Aldehydes release zinc from zinc- binding proteins ... measuring zinc binding and release, zinc coordination environments in zinc finger proteins, and redox sensitivity and activity of zinc- bound thiols Methods Enzymol 348, 230–237 Zeng J (1991) Lysine ... aldehydes on the zinc- binding capacity of zinc proteins was assayed by employing spectrophotometry and the chromophoric indicator 4-(2-pyridylazo)-resorcinol (PAR) for zinc ions Acrolein at concentrations...

Ngày tải lên: 19/02/2014, 06:20

... containing peripheral membrane proteins, a detergent-phase in which proteins with a-helical transmembrane domains are soluble and a ‘phospholipid-enriched’ pellet fraction containing proteins ... inner membrane However, since we identified no other inner membrane proteins in our vesicle preparation, we suggest that Gut2 is an outer membrane protein with its N-terminal FAD -binding domain ... size of proteins enriched in the lipid-rich pellet including the major 29 kDa protein Por1 and Tom40, identified by mass spectrometry and immunoblotting (C) After in situ digestion with trypsin, mass...

Ngày tải lên: 19/02/2014, 07:20

... cellular proteins but also in chromatin-bound proteins The Mcm proteins bound to chromatin probably function in DNA replication, but the role of the proteins not tightly bound to chromatin remains to ... comparing the amounts of Mcm in total cellular proteins and chromatin-bound proteins, approximately one-third of total Mcm protein was found to be recovered in the chromatin-bound fraction in HeLa ... protein After the band intensities were quantitated in each protein, the concentrations of these proteins in total and chromatin-bound fraction were determined The relative ratio (HeLa/WI) in the...

Ngày tải lên: 20/02/2014, 23:20