synthesis functionalization and applications in diagnosis and therapy

... AI054907 We thank Julie Morris, Sarah Akkina and Jennifer Quick for help with maintaining hES cells and culturing embryoid bodies We thank Leila Remling for isolating fetal CD34 cells We thank 19 20 ... capacity to ingest foreign particles in phagocytosis assays using E.coli Bioparticles® In addition to antigen presentation and phagocytosis, macrophages also play a critical role in inflammation and ... routine passaging of cells The cell clumps were incubated with the vector for hrs in the presence of polybrene ug/ml A secondary cycle of transduction was done by adding fresh vector and incubating...

Ngày tải lên: 13/08/2014, 09:21

... simplifying And- Inverter Graphs (AIGs) by systematically merging graph vertices in a topological fashion starting from the inputs, using a combination of structural hashing, simulation, and SAT ... hand, some points of I are covered only by cubes entirely contained in I Therefore keeping them both in I and in the projected subfunctions would be useless and expensive In our example, since ... highlights that in expanding a Boolean function we have two degrees of freedom: choosing the basis (in this case, the function g) and choosing one completely specified function included in the incompletely...

Ngày tải lên: 31/05/2014, 00:10

... cancer and ovarian cancer prognosis Use of Mucins in Radioimmunodiagnosis (RID) and Radioimmunotherapy (RIT) Monoclonal antibodies against mucins may have potential applications in improving the diagnosis ... cells [8284] At present, MUC1 and MUC16 are the best and only characterized mucins and monoclonal antibodies against MUC1 and MUC16 are under preclinical and clinical investigations for ovarian ... ovarian tumors and their potential role in ovarian cancer diagnosis and treatment Mucins Being that 90% of ovarian cancers are of epithelial origin, mucins may be attractive candidates for the...

Ngày tải lên: 20/06/2014, 07:20

... guitar tones using a combination of physical modelling and warped linear predictive coding is outlined in Section Section analyzes the results from simulated coding scenarios using the recorded ... sequencing program using only virtual instruments and software effects, the file size (including simple instrument and effect model algorithms) is on the order of 500 kB For WLP in Physical Modelling ... coding for MiniDisc, reprinted from the 93rd Audio Engineering Society Convention, San Francisco, Calif, USA, 1992 [19] J O Smith, “Efficient synthesis of stringed musical instruments,” in Proc International...

Ngày tải lên: 23/06/2014, 01:20

... emphasis on brain, prostate, pancreatic and colorectal cancers were included in the review Potential applications of curcumin in cancer prevention and therapy Curcumin exhibits in vitro and in vivo ... cancer cells and antitumoral effect on MDA-MB-231 cell xenografts in vivo by inhibiting p21WAP1, survivin and NF-B and its downstream effectors, including cyclin D1 and Bcl-2, and activating caspase ... cell lines and those detected in the patients in clinical settings Clinical trials of curcumin The results from phase I/II clinical trials including the dose-escalation studies with pure curcumin...

Ngày tải lên: 13/08/2014, 14:20

... nuclei in all panels were stained with DAPI and shown in blue 115  Figure 6.14: Immunostaining of Troponin I (green) and F-actin (red) in C2C12 cells cultured in ES PPyrPEC fibers (A) and ... cell nuclei in all panels were stained with DAPI and shown in blue 106  Figure 6.8: Immunofluorescent staining of desmin and connexin 43 in C2C12 cells cultured on PPyrPEC fibers in the presence ... cell nuclei in all panels were stained with DAPI and shown in blue 111  Figure 6.11: Immunofluorescent staining of α-actinin and MYH in C2C12 cells cultured on PPyrPEC fibers in the presence...

Ngày tải lên: 11/09/2015, 09:10

... University of Singapore for the award of research scholarship and Institute of Chemical and Engineering Science Ltd for the financial support to carry out the research work reported herein Finally, ... Teck-Chia, Ms Poon Yin-Fun and Dr Zhang Wei-Guang for their support and useful discussions; Dr Liu Shou-Ping, Mr Zou Yong, Mr Zhang Sheng, Xia Hai-Bing and Cheng Da-Ming for their advices and friendship ... Chapter Synthesis of positively charged single-isomer cyclodextrins 33 Approached for cyclodextrins 33 2 Synthesis of single-isomer positively charged cyclodextrins 37 2 Synthesis...

Ngày tải lên: 16/09/2015, 08:30

... entering (in whole or in part) the cavity and forming noncovalent host-guest inclusion complexes Almost all applications of CDs involve complexation The mechanism of inclusion complexation in CE ... recognition is obtained mainly by charge-charge interactions, hydrogen bonding, hydrophobic inclusion and π-π interactions These interactions can be either attractive or repulsive Proteins also have ... of both anions and cations in one single run, using CZE in moving boundary electrophoresis In moving boundary electrophoresis, the separation system is filled with a so-called leading electrolyte...

Ngày tải lên: 16/09/2015, 08:30

... successful use of zeolites in hydrocarbon processing, their use in the synthesis of organic intermediates and fine chemicals is in a relatively early state of development Two main reasons for this are: ... of large rings (rings of 12 oxygen atoms as the minimum constricting apertures) This gives zeolite beta interesting potential applications in catalytic reactions where high thermal and hydrothermal ... Chapter Introduction XX 1.1 General introduction 1.2 Synthesis of zeolite beta 1.2.1 Synthesis of zeolite beta in basic medium 1.2.2 Synthesis of zeolite beta in fluoride medium 1.2.3 Incorporation...

Ngày tải lên: 16/09/2015, 17:12

... with running buffer and the sample is introduced by dipping one end into the sample and applying an electric field (electrokinetic injection) or by applying gas pressure (hydrodynamic injection) ... strength in narrow capillaries Applications also include the separation of protein and peptides, tryptic mapping, DNA sequencing, serum analysis, analysis of neurotransmitters in single cells and ... pressure and longer columns than in HPLC can be used Nowadays, CEC is applied in separating a wide range of materials including phenols, PAHs, amines, carbonyls, dyes, and even inorganic and small...

Ngày tải lên: 17/09/2015, 17:19

... protein labeling in SDS-PAGE experiments 59 using probe 18 5.2.5 Combinatorial synthesis of peptide vinyl sulfone probe 59 via Rink-amide resin 5.2.6 SDS-PAGE experiments with combinatorial vinyl ... vinyl sulfone inhibiting cysteine protease Figure Positional scanning library in the generation of affinity 10 fingerprint of peptide epoxide inhibitors Figure Synthesis of Boc-Leu-vinyl sulfone ... Cathepsin K Different enzymes Data readout and computation process Affinity fingerprint Figure Positional scanning library in the generation of affinity fingerprint of peptide epoxide inhibitors...

Ngày tải lên: 06/10/2015, 20:49

... Modification of Proteins There is an increasing interest in protein conjugates for diagnosis and therapy Even though proteins often display limited pharmacokinetics, low proteolytic stabilities and the possibility ... Since free cysteine groups are rare and often inaccessible, they can be engineered into the protein as point mutations using molecular biological techniques Such approaches are usually demanding ... biomolecules, including peptides[16], proteins[17], antibodies[18], oligonucleotides[19] and biotin/avidin[20] These biomolecules are used as molecular imaging probes which show high binding affinity...

Ngày tải lên: 16/10/2015, 11:57

... coordinates for predicting sea level, currents in estuarine and shelf water (Muin and Spaulding 1996) Ye and McCorquodale (1997) developed a depth-averaged model in a non-orthogonal curvilinear system ... the advantage of reduction in computational cost while maintaining the accuracy when applied to flow in a channel with linear or almost linear bottom/bed But indeed, in many cases, water flows ... concept of simple Rankine vortex that including two parts: free vortex in outer zone and forced vortex in inner zone (Townson 1991)………………………………………33 Figure 4.5 Definition of coordinate components……………………………….36...

Ngày tải lên: 06/11/2012, 10:35

... oxide and potassium oxide It is fabricated by mixing titanium oxides and potassium oxides in a certain ratio, with a suitable calcination flux, and calcining at 900 – 1100°C The reaction during ... showed in Fig It can be seen that the absorption of Pb2+ and Cd2+ on PTW increases with time The absorption capacity of PTW increases quickly in the beginning and slows down gradually, and reaches ... granules, such as spherule or honeycomb, of PTW by mixing and calcining with a suitable binder material Research in this area is being done in Shandong University ACKNOWLEDGMENT This research was...

Ngày tải lên: 05/09/2013, 09:38

... products ethylene and propene In addition, the smother operation reduces the coking process in the crackers, resulting in a slight decrease of required cracker cleaning steps in a given interval Fuel ... training Figure 17 shows the NeuroFuzzy Module during training [8] [9] [10] Figure 17: fuzzyTECH NeuroFuzzy Module training Within two days of implementation work we generated the sample data and ... finally succeeded by using a combination of a fuzzy logic and In the polymerisation, the ingredients ethylene and a solvent react using a catalyst The quality of the final product depends on...

Ngày tải lên: 13/12/2013, 01:15

... products ethylene and propene In addition, the smother operation reduces the coking process in the crackers, resulting in a slight decrease of required cracker cleaning steps in a given interval Fuel ... training Figure 17 shows the NeuroFuzzy Module during training [8] [9] [10] Figure 17: fuzzyTECH NeuroFuzzy Module training Within two days of implementation work we generated the sample data and ... finally succeeded by using a combination of a fuzzy logic and In the polymerisation, the ingredients ethylene and a solvent react using a catalyst The quality of the final product depends on...

Ngày tải lên: 22/12/2013, 10:15

... following IMPACT-CN system manufactures instruction by using chitin beads column chromatography The obtained purified recombinant MCoTI-II showing inhibitory activity against trypsin ,and proteinases ... the 58 kD protein band was found in induced sample as the major band, acounted for 80% of total protein (lane 1,3,5 and 6) 58 kD Fig 10 SDS PAGE pattern of PI-17 proteins Mc Standard molecular ... Standard molecular markers 2, Uninduced 1, 3, 5, Induced (containing 58kD fusion protein band) Cells were broken by sonication in 10mM Tris-HCl buffer pH8 containing 1mM EDTA (TE buffer) After...

Ngày tải lên: 12/02/2014, 10:20

... times between starting at the point x0 y0 and landing in the treacle The function ‘stats[random,normald](1)’ creates a random standard normal deviate (b) Plot a histogram showing the distribution ... random number The seed is set using the command ‘randomize(integer)’ before invoking ‘rand()’ Maple also provides another U generator This is ‘stats[random,uniform] (1)’ This is based upon ‘rand’ ... necessary to test for the independence of the random variables, and, secondly, by dividing the domain into intervals, we are essentially testing against a discrete rather than a continuous uniform distribution...

Ngày tải lên: 07/03/2014, 15:20

... GAL4 DNA-binding domain with CfEcR ligand-binding domain cloned under the control of the 35S promoter in the pKYLX80 vector A fusion of VP16 activation domain and LmRXR ligand-binding domain cloned ... Interestingly, an increase in the ligandinduced activity of this switch containing the improved Kozak sequence was not accompanied by an increase in background activity in the absence of ligand Therefore, ... activity in the absence of ligand and lower induced luciferase activity in the presence of ligand The transgenic plants containing the CfEcR:LmRXR switch showed a similar meth- Time course studies in...

Ngày tải lên: 15/03/2014, 23:20

... lymphomagenesis in mice [44] The miRNA miR-21 is overexpressed in many solid tumors, including breast, colon, lung, prostate and stomach, and in endocrine pancreas tumors, glioblastomas and uterine leiomyomas ... of essential proteins responsible for accelerating the cell cycle, including cyclin D and retinoblastoma [56,57] Moreover, alterations in the machinery responsible for processing miRNA contributes ... examined to date [8,52], a feature that could be particularly useful in diagnosing incident cancers in otherwise normal tissues Indeed, this approach discriminates normal and neoplastic tissues in...

Ngày tải lên: 16/03/2014, 01:20