symptoms characteristics and consequences of dental anxiety and dental phobia
Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... wide variety of reference gene candidates The inconsistent data regarding to the Bestkeeper calculation of the coefficient of correlation and the corresponding p-values may be a result of the Pearson ... participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection ... elucidation of the acquired data http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence...
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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... wide variety of reference gene candidates The inconsistent data regarding to the Bestkeeper calculation of the coefficient of correlation and the corresponding p-values may be a result of the Pearson ... participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection ... elucidation of the acquired data http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence...
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Báo cáo y học: " Phosphodiesterase type 4 expression and anti-proliferative effects in human pulmonary artery smooth muscle cells" docx
... design and discussion of data JW: participated in the design and coordination of the project and writing of the manuscript Acknowledgements This work was supported by the British Heart Foundation and ... for 1–2 h and destained in fixing/destain solution until bands of activity were clearly visible The presence of MMP activity was confirmed by inhibition with 10 mM EDTA and the use of purified ... value of P < 0.05 was taken to be significant Results PDE4 gene expression and activity Products of 546, 506, 410 and 479 base pairs (bp), corresponding to fragments of PDE4A, PDE4B, PDE4C and...
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Báo cáo y học: " Ancient, independent evolution and distinct molecular features of the novel human T-lymphotropic virus type 4" pot
... intervals for the origin of HTLV-2 as it migrated out of Africa and Asia and into the Americas via the Bering land bridge and 40,000 – 60,000 ya as confidence intervals for the origin of HTLV-1 in Melanesia ... organization and characterization of the HTLV4(1863LE) structural and enzymatic proteins, and the LTR The genomic structure of HTLV-4(1863LE) was similar to that of other PTLVs and included the ... p30II, and p13II (where I and II denote ORFI and ORFII, respectively), have been identified in the pX region of HTLV-1 and are important in viral infectivity and replication, T-cell activation, and...
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AC R Sample Task Type 4 Task
... Write your answers in boxes 913 on your answer sheet. Species Size Preferred climate Complementary species Start of active period Number of generations per year French 2.5 cm cool Spanish late spring 1 2 Spanish ...
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A comparative study of discourse structures and some major linguistic features of international declarations and international conventions on human rights part 4
... right to a standard of living adequate for the health and well-being of himself and of his family, including food, clothing, housing and medical care and necessary social services, and the right ... the Charter of the United Nations, recognition of the inherent dignity and of the equal and inalienable rights of all members of the human family is the foundation of freedom, justice and peace ... law solely for the purpose of securing due recognition and respect for the rights and freedoms of others and of meeting the just requirements of morality, public order and the general welfare in...
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Tài liệu E-Human Resource Management 4 pptx
... between innovation, trust, and learning on the one hand and new forms of discipline and control on the other will not be easy They will require a refocusing of role for HR professionals HR roles have ... strategic change emphasize the importance of having an understanding of the organization climate and culture, and the current attitude of employees and management in order to assess the best ... are an appropriate means of looking at issues such as the clarity of corporate strategy and sharing of common values, communication and feedback processes, levels of trust and perceived organizational...
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Báo cáo khoa học: The Saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit R2 confers resistance to the anticancer drug cisplatin pot
... loadings of supernatant and pellet fractions were analysed by SDS ⁄ PAGE and immunoblotting with MYC antibodies (A) YBL046w–MYC13 and HA3– Pph3p (B) YBL046w–MYC13 and HA3–Sit4p (C) YBL046w–MYC13 and ... indicated concentrations of benomyl and grown at 26 °C for days of strains carrying deletions of PPH3, YBL046w ⁄ PSY4 and PSY2 Experimental procedures Yeast strains, plasmids, media and general methods ... deletion of the PSY4 ⁄ YBL046w gene using a single-step PCR-based method [24] with plasmid pFA6aHis3MX6 as template and source of the HIS3 gene and primers F1 and R1 (Table 2) Single-step tagging of...
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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
... understanding of the importance of Asn130 in substrate binding and recognition by arginase I, this residue was changed to aspartate by sitedirected mutagenesis, and the kinetic consequences of the ... existence of significant differences between the active sites of arginases I and II [22] Results and Discussion As shown in Table 1, upon mutation of Asn130 to aspartate, the arginase activity of human ... Agmatinase activity of the N130D mutant arginase I Fig Inhibition of wild-type and N130D variants of human arginase I by agmatine (A) and putrescine (B) The arginine concentration was mM and the assays...
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Báo cáo khoa học: Effect of heliquinomycin on the activity of human minichromosome maintenance 4/6/7 helicase pdf
... activities of the MCM4/6/7 complex (120 ng) and SV40 T antigen (200 ng) in the presence of single-stranded DNA The radioactivity at the sites to which Pi and ATP migrated was measured, and the ratio of ... formation of the MCM4/6/7 complex We also examined the sensitivity of the ATPase activities of the MCM4/6/7 complex and the SV40 T antigen to heliquinomycin in the absence of single-stranded DNA ... activity of MCM4/6/7 was also stimulated in the presence of M13mp18 singlestranded DNA in place of heat-denatured DNA, and the stimulated activity was also inhibited in the presence of heliquinomycin...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot
... determinations Error bars represent the standard deviation of these mean values Table Kinetic constants of human UDP-galactose 4-epimerase (GALE) and the consequences of disease-causing mutations Values ... [27], and thus the observed activity probably reflects that of the native enzyme With the exception of L183P, all the mutant proteins could be expressed and purified using similar conditions and ... twofold in any kinetic parameter) Again, these mutations are associated with the peripheral form of the disease [22,24,26] K257, L313 and G319 lie within 0.13 nm of each other on one face of...
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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot
... inoculum, after hpi and 1, 3, and dpi are shown Results are based on three experiments and presented as mean ± standard deviation (error bars) B and C Light microscopic analyses of HHV-6A- and mock-infected ... contributions MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped ... enlargement of cells, in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and mockinfected cells were fixed and stained for gp60/110 (red) and counterstained...
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Báo cáo sinh học: " The simultaneous presence and expression of human hepatitis C virus (HCV), human herpesvirus-6 (HHV-6), and human immunodeficiency virus-1 (HIV-1) in a single human T-cell" docx
... irrespective of infections and other associated problems [4] Our effort is to understand the impact of multiple infections at a cellular level, both on virus reproduction and its characteristics and on ... body and CIMM Authors' contributions K.A.S., A.G., and R.G performed biological work J.G.P and A.S.K performed the clinical work, recruitment of patients, and procurement of specimens K.A.S and ... artefact of fixation and processing Figure gels of PCR products Agarose3 Agarose gels of PCR products The DNA standard is a kb plus ladder (Invitrogen) Arrows indicate the expected band sizes...
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Báo cáo sinh học: " Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L" pot
... concentration of mM 3B) The data indicate that BPEOIA bound to the free thiol of cysteine residue(s) of L1 Binding of BPEOIA to the L1 cysteine residues of 16PV BPEOIA, capable of making a complex ... thiol of C146, C225, and C229 Some of the large tryptic fragments that bound to BPEOIA may not be detected because of their low recovery from LC and/ or inefficiency in the ionization Page of 11 ... that DTNB and NEM reduced the cellbinding ability of the 16PVs But the reduction of the binding efficiency does not fully account for the inhibition of the infectivity of the DTNB-16PVs and the...
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Báo cáo sinh học: " The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient c" pptx
... sequences of E5 and the mutants M1, M2 and M3 Transmembrane Hidden Markov Model posterior probabilities for the sequences of E5 and the mutants M1, M2 and M3 A) Amino acid sequence of the wild-type ... oncogenes HPV16 E5, E6 and E7 and different apoptotic pathways have already been identified [9] E6 and E7 modulate apoptosis by binding and inactivating p53 and the product of tumour suppressor ... impairs ligand-mediated apoptosis by reducing the amount of surface CD95 proteins or inhibiting the formation of the DISC complex [12], and affects the normal functioning of a number of membrane...
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Báo cáo sinh học: " Human herpesvirus 6A induces apoptosis of primary human fetal astrocytes via both caspase-dependent and -independent pathways" potx
... and WXH designed and directed studies, and were involved in the interpretation of the data and writing of manuscript BG, GFZ and FZ carried out the cells culture and HHV-6 infection BG, JFW and ... pathway and involves down-regulation of bcl-2 expression The translocation and accumulation of Bax, a pro-apoptotic factor of Bcl-2 family in mitochondria will lead to release of cytochrome c and ... in immunofluorescence assay and the western blot analysis BG, DJF and CZ performed the flow-cytometry experiments BG, JC and DDG performed the analysis of Caspase-8 and -9 activities and electron...
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Báo cáo sinh học: " Human herpesvirus 8 – A novel human pathogen" potx
... no large studies of the donor's and the recipient's HHV-8 serostatus and presence of HHV-8 in donor blood and organ Properly done, both antibody prevalence and a determination of infectious virus ... ras, c-myc, and p53 with prevalences of 30%, 25%, and 15–45%, respectively [146] The possible role of HHV-8 in MM has been debated and a full report of the evidence is beyond the scope of this review ... Prevalences of HHV-8 infection of 0.6% to 11.2%, 2.4%, and 40% where found in Thailand, India, and Taiwan, respectively [34,197,199] Classical KS still had the highest rate of infection, with 83% of patients...
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nghiên cứu phát hiện sự gắn chèn gene e6 và e7 của human papilomavirus type 16 vào bộ gene người bằng kỹ thuật multiplex rt-pcr
... X100): phenol 38%, guanidium thyocianate 0.8M, glycerol 5%, ch nh v pH 4.0 - Dung d ch 2: Chloroform 26 - Dung d ch 3: Isopropanol t i có ch a ch t tr t a - Dung d ch 4: Ethanol 70% - Dung d ... thi t k ánh giá m i Trong giai o n thi t k m i, qua tham kh o m t s tài li u v thi t k m i c a Andrew Wallace (1996), Alkami Biosystems (1999) , m t s tiêu chu n c a m i c rút sau: - Chi u dài ... bào thu nh n RNA t ng s tách chi t t bào thu nh n RNA t ng s , s d ng phương pháp t a phenol/chloroform theo phương pháp c a Chomczynski & Sacchi (1987) Quy trình tách chi t ã c thương m i hóa cung...
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Nuclear Power Control, Reliability and Human Factors Part 4 docx
... MIL-STD-461e1 Standard, Requirements for the control of electromagnetic interference, characteristics of subsystems and equipment, U.S Department of Defense IEC (2002) IEC 61000 Standard, Electromagnetic ... sensors of different types and is capable of analyzing the dynamics of environmental processes and predicting the most probable events The discussion includes analysis of: 1) the technical aspects of ... temperature, ionizing radiation of different types, gas molecules and ion concentrations, vapors, and presence of dust particles The overlapping of all these maps and reconstruction of their dynamics can...
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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx
... inoculum, after hpi and 1, 3, and dpi are shown Results are based on three experiments and presented as mean ± standard deviation (error bars) B and C Light microscopic analyses of HHV-6A- and mock-infected ... contributions MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped ... enlargement of cells, in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and mockinfected cells were fixed and stained for gp60/110 (red) and counterstained...
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