swelling of polyheteroarylenes synthesized in n methylpyrrolidone

... involved in the binding of UDPGal and that E264 and W315 are involved in the GlcNAcbpNP binding site The residue E264, which is part of the conserved EDV motif, is involved in both substrate binding ... this context, it is worth noting that DXD is found in inverting and noninverting transferases, which add different sugars to other sugars, phosphates and proteins However, these DXDcontaining glycosyltransferases ... CATCTCAGATGCTTCTTGCTTGAC-3¢ introducing a SalI and XbaI site, respectively The W315A and C326A mutants were generated through single PCR reactions using the 5¢ restriction site-containing primer and an antisense oligonucleotide...

Ngày tải lên: 08/03/2014, 16:20

... however, nothing is yet known on further downstream components for the signals that originate in a tetraspanin antigen In this report we have shown that ligation of CD53 antigen, in rat and human cells, ... expression vectors encode fusion proteins containing the GAL4 DNAbinding domain and the c-Jun activation domain (residues 1–223) in wild-type and mutant forms (not phosphorylatable by mutation of both ... control of the cytomegalovirus promoter The clone was named pCEFL-KZ-CD53 For experiments using exogenous Jun kinase, a clone with the N- terminus of Jun kinase containing the haemagglutinin (HA)...

Ngày tải lên: 08/03/2014, 22:20

... ferritin homolog A B C Artemin, apoferritin and ferritin inhibit citrate synthase denaturation Artemin, apoferritin and ferritin protected citrate synthase against denaturation at 43 °C in a concentrationdependent ... 90% of artemin-containing cells endured a 30 heat shock, as opposed to only 17% of those lacking the protein (Fig 4A) In comparison, 55% of cells containing artemin survived incubation in 0.50 ... than by indiscriminate binding to denaturing proteins In this vein, Artemia p26 inhibits apoptosis in transfected mammalian cells [12] On the basis of these observations, artemin has the potential...

Ngày tải lên: 16/03/2014, 11:20

... having N- linked oligosaccharide chains interact noncovalently with nascent H-chains, perhaps during their translation and translocation into ER, as a sort of molecular chaperone to prevent denaturation ... Role of L-chain in the elementary unit of fibroin (Eur J Biochem 271) 361 Table Determination of molar ratios of H-chain, L-chain, and fhx/P25 in the fibroin secreted into cocoons of a normal breed ... specific antibodies as described [1] Construction of a recombinant transforming plasmid and production of transgenic silkworms A 5¢-flanking region ()600 to +34) of the L-chain gene containing a putative...

Ngày tải lên: 16/03/2014, 16:20

... Table N- terminal sequence of N- myristoylated proteins having Lys residue at position Amino acids at positions 3, and are in bold type Protein Ca2+ binding/EF hand proteins Aplycalcin Hippocalcin Neurocalcin ... a distinct position in the substrate protein and elements in the enzyme’s peptide-binding site will induce changes in the structure of the peptide-binding site that cause an alteration in the ... position in the N- myristoylation consensus motif on protein N- myristoylation, vertical scanning mutagenesis of the amino acid at position in MG6S-TNF was performed; a series of TNF mutants in which...

Ngày tải lên: 16/03/2014, 16:20

... members all contain the highly conserved ETS domain ERM contains two DNA binding inhibitory domains flanking the Ets domain: the central 87 amino acid inhibitory domain is poorly conserved and virtually ... corresponds to an unknown binding protein Arrowheads indicate the position of DNase I hypersensitive sites observed in footprinting experiments The Ets and Sp1 consensus motifs are underlined Arrows ... recognition of target sites in different genes In addition promoter sequences flanking the Ets motif determine activity through protein–protein interactions between Ets factors and factors binding...

Ngày tải lên: 16/03/2014, 18:20

... chemiluminescence (CL) method Representative data showing inhibition of CL by one compound, selenourea A, are shown in Fig This compound incubated with PMNs significantly inhibited CL in a dose-dependent ... compounds not significantly inhibit xanthine oxidase activity [38–40] In the present study, we investigated the anti -in ammatory ⁄ antioxidant potential of these compounds by examining their scavenging ... HBSS (pH 7.4) containing mm NaN3, and then incubated at 37 °C in an atmosphere 6052 of 95% air and 5% CO2 in the presence of each organic selenium compound or SOD After min, PMNs were stimulated...

Ngày tải lên: 23/03/2014, 07:20

... majority of SiallNnT-containing glycoforms contain two PEtn residues In the inner core of H in uenzae LPS, one PEtn residue is stoichiometrically present at the 6-position of the Hep II residue, and ... mutant that had been grown on media not containing Neu5Ac Under these growth conditions, the RM118 lpsA mutant does not elaborate detectable amounts of LPS containing sialylated glycoforms Fig Ring ... and mutants causing sequential truncations from Hep III are illustrated in Fig DISCUSSION Structural analysis of H in uenzae strain RM118 lpsA LPS has revealed glycoforms containing a Sial-lNnT...

Ngày tải lên: 23/03/2014, 21:21

... Ca2+-binding EF-hand family protein Protein kinase Zinc-finger (C3HC4-type RING finger) family protein Zinc-finger (C3HC4-type RING finger) family protein Glutaredoxin family protein Neurofilament triplet ... are present at both positions and 3, indicating that the initiating Met is retained in Myr– AGG1-3P6A and Myr–AGG1-3P6S This result is in good agreement with our previous finding of an inhibitory ... (where Xaa indicates any amino acid), at their N- terminus [18] This motif interacts with the substrate-binding pocket of NMT, and thereby ensures binding of the substrate protein to the enzyme [19]...

Ngày tải lên: 29/03/2014, 21:20

... carbohydrate-dependent manner Mixtures of lactose and (asialo)fetuin were expected to be potent inhibitors Carbohydrate-independent binding of the neoglycoprotein and biotin-independent binding of the second-step ... median signal of binding of the fluorescent probe is given as extent of carbohydrate-ligand-dependent binding by subtracting carbohydrate-independent binding from total binding for each cell line BiB10-BSA ... is known from the plant lectin erythroagglutinating phytohemagglutinin (E-PHA), whereas the presence of a bisecting GlcNAc in a core-fucosylated biantennary N- glycan did not affect binding to...

Ngày tải lên: 30/03/2014, 13:20

... presequence of pCYP11A1 can be somehow involved in confining this protein in the inner mitochondrial membrane This specific presequence has an N- terminal region enriched in hydrophobic amino acid residues, ... SDS/PAGE and Western blotting of the samples containing equal amounts of total protein According to these data, the content of the imported protein in the supernatant was 0.025% of total protein of ... mCYP11A1 and a minor amount of the precursor were found in the pellet mainly containing the inner mitochondrial membrane fragments (sonic SMP) In the case of mitochondria from yeast cells expressing...

Ngày tải lên: 31/03/2014, 07:20

... N- terminal cleavage fragment of Ac45 did not accumulate when NILs were incubated in the presence of monensin or the lysosomal inhibitors leupeptin, chloroquine and Baf However, in NILs incubated in ... immunoprecipitates contain 37-kDa POMC and  70 kDa PC2 that bound nonspecifically (asterisks) from adopting its normal conformation or from interacting with its binding partner Tunicamycin inhibits N- linked ... during the chase incubation In some experiments, lobes were first incubated overnight in the absence or presence of 10 lgÆmL)1 tunicamycin in culture medium containing 10% fetal bovine serum (Gibco-BRL)...

Ngày tải lên: 31/03/2014, 09:20

... is not r-continuous function in X × K Let us emphasize that the point of Ω(x, k)-bifurcations can be not the point of r-discontinuity Now, suppose that ω(x, k) is not r-continuous in X × K Then ... than in general case [8, 58] They possess a number of characteristics which not remain in higher dimensions In particular, for them the set of non-wandering points consists of a finite number of ... note an important property of uniform convergence in compact intervals Let (xn , kn ) → (x∗ , k ∗ ) and all (kn , xn )-motions and correspondingly (k ∗ , x∗ )motion be determined in compact interval...

Ngày tải lên: 24/04/2014, 16:50

... increased after annealing in air, oxygen ambients, and decrease in argon ambient However, in nitrogen ambient, the EL emission of the red peak in the range of 690–750 nm was increased, and in ... at an injection current of mA for the as grown and annealed ZnO NTs in different ambients under forward bias of 25 V and it shows the shift in emission peak after annealing in different ambient ... chromaticity space of ZnO nanotubes, for (a) as grown, (b) annealed in argon, (c) annealed in air, (d) annealed in oxygen, (e) annealed in nitrogen, and (f) all together Alvi et al Nanoscale Research...

Ngày tải lên: 21/06/2014, 05:20

... permutations and convexset, and has a nonempty interior set Ω0 Let ϕ : Ω → R be continuous on Ω and continuously differentiable in Ω0 Then, ϕ is a Schur-convex (Schur-concave) function if and only ... Shandong Science and Technology Press, Jinan, China, 3rd edition, 2004 [14] D S Mitrinovi´ , Analytic Inequalities, vol 1965 of Die Grundlehren der mathematischen Wisenc schaften, Springer, New ... and I Olkin, Inequalities: Theory of Majorization and Its Applications, vol 143 of Mathematics in Science and Engineering, Academic Press, New York, NY, USA, 1979 [13] J.-C Kuang, Applied Inequalities,...

Ngày tải lên: 22/06/2014, 06:20

... existence of inherited ovals in the dual plane of H(q ), which is a Moulton plane M (q ) of the same order Moulton planes have been originally introduced in [10], by altering some of the lines of ... union of two half–lines of different slope with one point in common This construction, when considering planes of finite order q , may be carried out as follows Let || · || denote the norm function ... on Innovations in Incidence Geometry [2] C.J Colbourn, J.H Dinitz, Handbook of Combinatorial Designs (2nd ed.), Chapman & Hall (2007) [3] P Dembowski, Finite Geometries, Springer (1968) [4] N. L...

Ngày tải lên: 07/08/2014, 15:22

... treatment of 2-amino-3,8-dimethylimidazo[4,5-f] quinoxaline in gdp delta transgenic mice caused the increase of G : C to T : A trasversion in both time- and dose-dependent manner Their findings ... material-associated mutagenicity Ozone, NNK, DBP, and combined treatment of NNK and DBP on ozone were assayed for the mutant frequency of 6thioguanine-resistant (TGr) spleen lymphocytes in male and female mice ... spleens of mice following exposure to ozone, NNK, and DBP, and combined treatments of NNK and DBP on ozone for 32- and 52-wk The procedures for isolating lymphocytes from spleen and culturing hprt...

Ngày tải lên: 07/08/2014, 18:20

... compounds including lunasin and lectins that were shown to induce apoptosis in malignant cells [13] As CKBM contains these compounds, this would partially explain why it inhibits tumor growth in ... Schisandra chinensis has been shown to reduce prostate cancer cell growth and induce apoptosis by inhibiting androgen receptor expression [1] Besides, soybeans contain various anti-carcinogenic ... could induce cytokine release including TNF-α in these animals and inhibit tumor growth However, further study is needed to determine how they are induced and the mechanisms they inhibit cancer...

Ngày tải lên: 08/08/2014, 18:20

... 3D) In shoots, the total 1 5N content per DW g was lower in spinach than in pea plants (Figure 3A and 3C) The content of 1 5N in spinach shoots was higher in pH than in pH (Figure 3A), whereas in ... accumulation in NH4+- and NO3 fed plants at the same N concentration was dependent on the N concentration in the root medium and on the plant species concerned (Table 2) The degree of the effect of the ... symbiotic N2 fixation and uptake of NO3- by plants Plant Physiol 1980, 66:51-56 Kolb KJ, Evans RD: Influence of nitrogen source and concentration on nitrogen isotopic discrimination in two barley genotypes...

Ngày tải lên: 11/08/2014, 11:20

... ultrasound examination and Table Test characteristics of ultrasound examination, modified Boston examination, NT-proBNP and combination of ultrasound examination and NT-proBNPa Ultrasound examinationb ... (cutoff point: total points), NT-proBNP (cutoff point: 1,000 pg/mL) and a combination of ultrasound examination with NTproBNP In comparing the methods, we found significant differences between ultrasound ... ultrasound and NT-proBNP in differentiating HF-related acute dyspnea from COPD/ asthma-related acute dyspnea in prehospital settings Conclusions Ultrasound examination of the lungs alone or in combination...

Ngày tải lên: 14/08/2014, 08:21