summary of governing equations
Interactions between early germ cells and inner germarial sheath cells in drosphila ovary
Ngày tải lên :
14/09/2015, 08:42
... μL of reverse primer (10 μM) and μL of dNTPs (2.5 mM each) and μL of PCR buffer (for use of Pfx enzyme, additional μL of 50 mM MgSO4 was required), 37 μL of double distilled water (for use of ... included μL of x SYBR green mix, 0.5 μL of cDNA, μL of forward primer (10 μM), μL of reverse primer (10 μM) and 2.5 μL of double distilled water, and Q-PCR was carried out in a device of 7900HT ... IGS cells in med20RNAi Germaria 103 4.4 Requirement of specific Components of Mediator Complex in IGS cells .106 4.5 Microarray Profiling of med20RNAi IGS Cells 106 4.5.1 Introduction ...
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Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx
Ngày tải lên :
19/02/2014, 00:20
... genes, but also in the up-regulation of Oct4, a definitive marker of pluripotency, accompanied by a shorttermed drop of p38 phosphorylation levels [27] The mediators of these effects remain unidentified, ... BMP signaling in stem cells tolerate lack of ALK3 in absence of the inhibitor Although functional proof is missing, the authors found up-regulation of ALK1 and ALK2 in these cells and suggest ... signaling induces differentiation of neural stem cells Another variant of signal integration between BMPs and STAT3 has been identified in the case of differentiation of neural stem ⁄ progenitor cells...
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Báo cáo y học: "Global analysis of alternative splicing regulation by insulin and wingless signaling in Drosophila cells" ppt
Ngày tải lên :
14/08/2014, 21:20
... the average of log2 ratios for all the probes of a locus The net expression of a splice junction was calculated by subtracting the average log2 expression of all junctions of an isoform from the ... to some of the alternatively spliced regions frequently detects the existence of additional, non-annotated isoforms (data not shown) Functional overlap of genes regulated at the levels of transcription ... sets of genes [38,65,66] For example, analysis of pairs of major mouse tissues using genome-wide splicingsensitive microarrays concluded that only 15-20% of genes regulated at the level of splicing...
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Báo cáo khóa học: ICAM-1 expression is highly NF-jB-dependent in A549 cells No role for ERK and p38 MAPK docx
Ngày tải lên :
07/03/2014, 15:20
... phosphorylate downstream cofactors including CBP/p300 and positive cofactor to increase the transactivational potential of NF-jB [10,14] The pulmonary epithelium is the primary site of contact with airborne ... constitutive inhibitor of NF-jB As previously described, cells were infected at a multiplicity of infection (MOI) of 10, a level which we have shown previously to infect > 95% of cells, and then ... apparent by h poststimulation and levels of ICAM-1 protein continued to rise over the 18 h of the experiment To examine the role of NF-jB in the induction of ICAM-1, cells were infected with an...
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Báo cáo khoa học: Mammalian 105 kDa heat shock family proteins suppress hydrogen peroxide-induced apoptosis through a p38 MAPK-dependent mitochondrial pathway in HeLa cells potx
Ngày tải lên :
16/03/2014, 06:20
... forms of caspase-3 and a cleaved fragment of PARP were detected in cells treated with H2O2 Furthermore, overexpression of Hsp105a or Hsp105b markedly reduced both the amount of the active forms of ... cleavage of distinct and vital proteins [21–23] In particular, caspase-3 is one of the key executioners of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of ... overexpression of mouse Hsp105a or Hsp105b was induced by removing DOX from the culture medium In HeLa-tet ⁄ Hsp105a cells, the level of Hsp105a was increased approximately twofold by removal of DOX...
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Báo cáo khoa học: Intrabodies against the EVH1 domain of Wiskott–Aldrich syndrome protein inhibit T cell receptor signaling in transgenic mice T cells potx
Ngày tải lên :
16/03/2014, 14:20
... rate of capping of unstimulated and stimulated T cells was determined by counting the number of caps in 200 cells ⁄ experiment Subcellular localization of scFv intrabodies Cell extracts of anti-WASP ... appropriate cDNA fragments of the VH and VL regions were then generated by RT-PCR A comparison of the VH and VL amino-acid sequences of clones 18 and 21 is shown in Fig 1B All of the VH regions and ... 28.8 T7-WASP15 22 Fig Expression of anti-WASP scFvs and detection of their binding activity to WASP in T cells (A) Western blot analysis of protein extracts of anti-WASP scFv DNA-transfected...
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Báo cáo khoa học: Liver receptor homolog-1 localization in the nuclear body is regulated by sumoylation and cAMP signaling in rat granulosa cells docx
Ngày tải lên :
23/03/2014, 06:20
... 3h SAE1 600 % of control ** ** % of control 10 000 % of control Cyp11a1 30 000 % of control % of control A F.-M Yang et al h 12 h 24 h Fig The cAMP pathway regulates the expression of SUMO pathway ... sumoylated forms of LRH-1 in COS-7, 2.5 · 106 cells in 100 lL of serum-free medium were placed in a mm cuvette (Bio-Rad, Hercules, CA, USA) A total amount of lg of DNA (2 lg of Myc-tagged LRH-1 ... the effect of cAMP signaling on SUMO pathway is complex and cell type dependent Notwithstanding, the up regulation of E1, the down regulation of E2 or E3 and the increased expression of SENP2 lead...
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báo cáo hóa học:" Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ ADAM17 gene expression during in vitro maturation of porcine COCs" doc
Ngày tải lên :
20/06/2014, 07:20
... the phosphorylation of ERK1/2 and the meiotic resumption of oocytes [13] In this study, the addition of EGF to PKA Page of (page number not for citation purposes) Journal of Ovarian Research ... H89, SB203580 or U0126 on rate of oocyte exhibiting GVBD Effect of4 Effect of H89, SB203580 or U0126 on rate of oocyte exhibiting GVBD Values are mean +/- SEM of replicates *: The significant ... overcame the negative effect of each inhibitor on these gene expressions (Figure 3A, B, C) Effect of each specific inhibitor of PKA, p38 MAPK and MEK on meiotic resumption of oocytes and cumulus expansion...
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Báo cáo sinh học: "Compound developmental eye disorders following inactivation of TGF signaling in neural-crest stem cells" doc
Ngày tải lên :
06/08/2014, 18:21
... Journal of Biology 2005, Volume 4, Article 11 Ittner et al Background Normal functioning of the eye is dependent on a variety of highly specialized structures in the anterior segment of the eye ... patterning of the chamber angle between the cornea and the iris; it is also associated with a high prevalence of glaucoma [1] Development of the anterior eye segment depends on the proper function of ... mapping of NC-derived, -galactosidase (Gal)-expressing cells (blue) reveals (d) the NC origin of corneal keratocytes (arrows) and of corneal endothelium (arrowhead) (e) Structures of the chamber...
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Báo cáo y học: "Targeting Toll-like receptor signaling in plasmacytoid dendritic cells and autoreactive B cells as a therapy for lupus" pps
Ngày tải lên :
09/08/2014, 07:20
... mice) that are capable of activating the complement system [48] This may explain the predominance of these isotypes in animal models of lupus and the well known contribution of complement-mediated ... to respond to any of the above ligands [108] (Brummel and coworkers, unpublished data) Enhanced responsiveness of MZ-B cells was due to the combination of increased numbers of MZ-B cells in lupus ... Lenert Figure The innate model of lupus pathogenesis: central role of TLR-activated MZ-B cells and pDCs Presented is a schematic overview of innate activation of MZ-B cells and pDCs with hypomethylated...
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Endofin is a novel component in EGR EGFR oncogenic signaling
Ngày tải lên :
11/09/2015, 10:00
... localization of Endofin are necessary for its tyrosine phosphorylation 69 3.1.6 EGF-dependent co-localization of Endofin with EGFR requires a functional FYVE domain 3.2 Characterization of Endofin phosphorylation ... Schematic diagram of receptor endocytosis 31 1.9 Schematic representation of the various domains and interacting partners of Endofin 46 3.1.1 Tyrosine phosphorylation of Endofin occurs upon TGF-α ... Co-localization of Endofin with EGFR requires its FYVE domain 77 3.2.1A-B Y515 of Endofin is a major phosphorylation site for EGFR 82 VI 3.2.1C-D Endofin is a direct substrate of EGFR 83 3.2.2A-B...
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The study of interactions of transmembrane receptors and intracellular signaling proteins in live cells by fluorescence correlation and cross correlation spectroscopy
Ngày tải lên :
14/09/2015, 14:25
... support in my pursuing for MSc and PhD i Table of Contents Acknowledgements i Table of contents ii Summary v List of Tables viii List of Figures ix List of Symbols xi Chapter Introduction 1.1 Methods ... Construction of EGFR-FP fusions 48 3.1.2 Construction of ErbB2-EGFP and ErbB2-mRFP 51 3.1.3 Construction of mRFP-EGFR and mRFP-EGFR-EGFP 52 3.1.4 Construction of PMT-FPs 54 3.1.5 Summary 56 3.2 ... percentages of interacting proteins and the determination of the equilibrium dissociation v constants of binding proteins The experimental setups of FCS/SW-FCCS are introduced in the last section of this...
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Dysfunctional signaling pathway for nitric oxide production in endothelial cells chronically exposed to high glucose or high fatty acids
Ngày tải lên :
15/09/2015, 17:09
... seriously threatening factor to the living quality of these patients Therefore, prevention of long-term complications of diabetes is one of the major aims of treatment Consequently, it is necessary to ... threatening factor to the living quality of these patients Therefore, prevention of long-term macrovascular complications of type diabetes is one of the major aims of treatment Consequently, it is necessary ... THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NATIONAL UNIVERSITY MEDICAL INSTITUTE NATIONAL UNIVERSITY OF SINGAPORE 2005 ACKNOWLEDGEMENTS A number of people have contributed directly...
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Expression of neurogranin tagged with enhanced green fluorescence protein in HEK293 cells and its effects on neuronal signaling
Ngày tải lên :
05/10/2015, 22:32
... BamHI and HindIII in a total volume of 250 µl including 150 µl of purified PCR product, 10 unit of HindIII, 10 unit of BamHI, 25 µl of Buffer E (Promega), 2.5 µl of 10 mg/ml BSA (Bovine Serum Albumin) ... trifluoroacetic acid viii LIST OF FIGURES Fig.1 IQ motif of RC3/Ng and GAP-43… … … … … … … … … … … … … … … … … 12 Fig.2 Schematic representation of the role of Ng in the modulation of free Ca2+ and Ca2+/ ... of Ng is Ser36 which was determined by automatic sequencing of major radioactive tryptic peptide after trypsin digestion of the phosphorylated protein In addition, none of the Ser36 mutants of...
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Expression of neurogranin tagged with enhanced green fluorescence protein in HEK293 cells and its effects on neuronal signaling
Ngày tải lên :
06/10/2015, 20:35
... BamHI and HindIII in a total volume of 250 µl including 150 µl of purified PCR product, 10 unit of HindIII, 10 unit of BamHI, 25 µl of Buffer E (Promega), 2.5 µl of 10 mg/ml BSA (Bovine Serum Albumin) ... trifluoroacetic acid viii LIST OF FIGURES Fig.1 IQ motif of RC3/Ng and GAP-43… … … … … … … … … … … … … … … … … 12 Fig.2 Schematic representation of the role of Ng in the modulation of free Ca2+ and Ca2+/ ... of Ng is Ser36 which was determined by automatic sequencing of major radioactive tryptic peptide after trypsin digestion of the phosphorylated protein In addition, none of the Ser36 mutants of...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
Ngày tải lên :
25/10/2012, 11:18
... Fig.10 Mutation of 303PolyC in the mtDNA D-loop region a: Sequence of 303PolyC in the mtDNA D-loop region of AGS cells, the section of black line is the normal sequence; b: Sequence of 303PolyC in ... Sequence of 16184PolyC in mtDNA region of AGS cells, the section of black line is the sequence; b: Sequence of 16184PolyC in mtDNA http://www.medsci.org Int J Med Sci 2011, 64 D-loop region of AGS ... D-loop region of AGS cells, the section of black line is the normal sequence; b: Sequence of 514CA repeats in mtDNA D-loop region of AGS cells stimulated by Hp extracts, and the section of black line...
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Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
Ngày tải lên :
31/10/2012, 15:12
... transfection of Bag-1 Morpho/AS to JAR cells did not alter the expression of PTEN after the exposure to hypoxia, which would mean that the expression of PTEN, and also that of Bcl-X, was independent of ... time-dependent manner (Fig 1) that 22 of those proteins were significantly increased or decreased after hypoxia treatment A summary of the PowerBlot expression data of proteins detected is listed in ... expression profiles of proteins involved in the mitochondrial (bcl-2, bax, and bcl-x, etc.) and stress reaction-related pathways of apoptosis (Fig 2) Table Altered protein expression of JAR cells...
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Báo cáo y học: " Low concentration of ethanol induce apoptosis in HepG2 cells: role of various signal transduction pathways"
Ngày tải lên :
31/10/2012, 15:37
... numbers of cells with apoptosis-associated alterations of the nuclei and without membrane barrier dysfunction were determined within a field of view at a magnification of X400 A total of 10 randomly ... inhibition of the GPCR signaling pathway as shown by phosphorylation of GRK2 and PKCα; with values of 109% and 104%, respectively, combined with dephosphorylation of ROKα, PKCδ and PKCμ; with values of ... mechanism of ethanol-induced apoptosis Figure Effect of inhibitors of G-protein coupled receptors (GPCR) on ethanol-induced apoptosis in HepG2 cells Results are expressed as percent of control...
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Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
Ngày tải lên :
31/10/2012, 16:49
... variants (TV) of exon of the hCDC4 gene Arrows mark the position of the detected mutation in the 5’ UTR of Exon 1-TV3 and the new intronic SNP downstream of exon 10 (B) Chromatograph of the mutation ... Chromatographs of the different variations of the new intronic SNP in the hCDC4 gene on chromosome 4, position 153602874 (F) Gel electrophoresis of a Surveyor nuclease digested PCR product of the AML ... peripheral blood of AML patients at the time point of initial diagnosis This resulted in the identification of one heterozygous mutation in the 5’ untranslated region (5’UTR) of Exon (transcript...
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Báo cáo y học: "PKC and PKA Phosphorylation Affect the Subcellular Localization of Claudin-1 in Melanoma Cells"
Ngày tải lên :
03/11/2012, 11:17
... isoforoms, increased the expression (8) and cytoplasmic distribution of claudin-1, and simultaneously reduced tight junction barrier integrity (7) Conversely, activation of atypical PKC isoforms ... inhibitors of conventional PKC isoforms, implying that, in melanoma, claudin-1 expression is dependent upon PKC isoforoms such as α, β and γ (4) In this study, we also examine the effects of PKA ... of claudin-1 may dictate the metastatic capacity of melanoma cells Our findings suggest that nuclear versus cytoplasmic expression of claudin-1 94 may become a valuable marker for diagnosis of...
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