suitable bioreactors for plant cell and tissue cultures

... dhfrCell line Sf9 Cell line Schneider-2 Cell lines COS 1/COS Cell line NIH3T3 Cell line HeLa Cell line J558L Cell line Vero Myeloma cell lines Hybridomas Cell line MRC-5 Cell line WI-38 Cell ... Namalwa Cell line BHK-21 Cell line MDCK Cell line GH3 Cell line 293 Cell line !VCRE/!VCRIP References 1.3 Master and Working Cell Banks Scale and composition of cell banks Extended cell bank The cell ... used for paper production Contents Contributors Foreword Preface Safety CHAPTER THE CELL: SELECTION AND STANDARDIZATION 1.1 Overview References 1.2 Cell Lines for Biotechnologists Introduction Cell...

Ngày tải lên: 10/04/2014, 11:12

... INTRODUCTION TO CELL AND TISSUE CULTURE: Theory and Technique Jennie P Mather and Penelope E Roberts Page iii Introduction to Cell and Tissue Culture Theory and Technique Jennie P Mather and Penelope ... century formed with cells, including production of genetically altered cell lines through mutagenesis and cloning, direct comparison of cells from normal and transformed tissues, the study of cellular ... 12: Cell Culture for Commercial Settings Glossary Appendix Index 117 129 151 165 175 195 205 Page i Introduction to Cell and Tissue Culture Theory and Technique Page ii INTRODUCTORY CELL AND...

Ngày tải lên: 11/04/2014, 09:48

... signals and a permissive microenvironment, e.g., for cell homing Without manipulation of the stem cell niche either by cell transfection or addition of appropriate chemokines and growth factors and ... different subsets, i.e CNS stem cells and neural crest stem cells The latter give rise to neurons and glia of the peripheral nervous system and other connective cell types The subventricular zones ... shown for example by Zhu et al who transfected fetal neural stem cells with VEGF gene [73] After transplantation, the stem cells migrated and expressed VEGF during the early time after transplantation...

Ngày tải lên: 09/08/2014, 10:21

... attempts to understand hormone pathways as individual and combinatorial modulators of plant responses deposited research Figure Auxin and brassinosteroid regulate transcription mediating cell expansion ... NSF (DBI-0115870), and DOE (DE-FG02-02ER15312) References Clouse SD, Sasse JM: Brassinosteroids: essential regulators of plant growth and development Annu Rev Plant Physiol Plant Mol Biol 1998, ... microdissection of cells from plant tissues Plant Physiol 2003, 132:27-35 D’Haeseleer P, Liang S, Somogyi R: Genetic network inference: from co-expression clustering to reverse engineering Bioinformatics...

Ngày tải lên: 14/08/2014, 14:21

... isolates WE54, LCMV Cl13, and Traub efficiently target antigen presenting cells (dendritic cells) in the spleen and perturb their ability to present antigen to T cells and B cells, resulting in a ... TU), and FIV-GP64 (1.0 × 107 TU) For tissue staining studies, the approximate transducing units delivered were 5.0 × 10 for FIV-WE54-ntLacZ, 4.0 × 106 for FIV-L260F-ntLacZ, and 2.0 × 107 for FIVGP64-ntLacZ ... a-DG or BSA for hour on ice The inoculum was added to 90% confluent cultures of HEK293H cells (ATCC, CRL1573) in 8-well LabTeks plates (Nunc) and incubated for 45 minutes at 37°C/5% CO Cells were...

Ngày tải lên: 14/08/2014, 19:22

... 4-diamidino-2-phenylindole (DAPI) for staining the cell suspension was performed Each histogram represents 30.000 cells for measuring DNA-index and cell cycle For histogram analysis we used the ... Sci 2009, 341 Figure Constitutional formula of the investigated TMZ-BioShuttle Cell Cycle and Cell Death Analysis The effects on the cell viability and the cell cycle distribution were determined ... as internal standard for determination the diploid cell population The mean coefficient of variation (CV) of the diploid lymphocytes was 0.8 - 1.0 Cell viability and apoptotic cells were assessed...

Ngày tải lên: 26/10/2012, 09:48

... status of cells is now routinely performed for plant cell cultures in order to permit accurate assessment of growth and metabolite production rates The availability of plant cells for quantitative ... constituents on the cells is, however, possible Therefore, it is not possible to use a universal screening platform for plant cell cultures Instead, a specific screening for a particular plant cell culture ... accumulation and storage of desired secondary metabolites in plant cells Secondary metabolites in plants, and perhaps in tissue cultures, are usually stored intracellularly, as for example, in...

Ngày tải lên: 25/10/2013, 05:20

... [6–9] CD98 expression is also necessary for virus-induced cell fusion and for osteoclast formation [10–12] and, importantly, it is found in cytotrophoblasts and on the plasma membrane of the syncytiotrophoblast ... cells (B) Labeling of surface and intracellular antigens after cell permeabilization n ¼ number of cell samples A1 B A2 Fig Detection of partially glycosylated and unglycosylated CD98 after cell ... anti-rabbit IgG and DAB A novel band (arrow) of  64 kDa (A1) or  80 kDa (B) was present in whole cell lysates from BFA-treated cells in both control and forskolin-stimulated cells, presumably...

Ngày tải lên: 23/03/2014, 09:20

... regulation and processing of plant miRNAs and provide useful knowledge for understanding the mechanism of the origin and computational identification of new miRNAs in plants 930 Results and Discussion ... to understand transcriptional regulation [11,13–16], similar analyses have been performed only for a relatively small number of miRNAs in plants, and these were limited to A thaliana and O sativa ... conservation may be useful for understanding the mechanism of the origin of new plant miRNAs Materials and methods Data sets To obtain the upstream and downstream sequences of plant miRNA genes, we...

Ngày tải lên: 28/03/2014, 23:20

... this level of testing The decrease of cell positivity for KG-1 cells and rather constant results for K-562 cells can be judged as internal controls for cell- type specificity in the direct comparison ... their ligand properties for purified lectins, for tumor cells in vitro, and in tissue sections, and their biodistribution in tumor-bearing mice Bioconjugate Chem 8, 845–855 ´ 13 Unverzagt C, Andre ... using purified plant and mammalian lectins and wild-type and lectin-gene-transfected tumor cells as targets Bioconjugate Chem 15, 87–98 1998 ´ S Andre et al ´ 53 Morris S, Ahmad N, Andre S, Kaltner...

Ngày tải lên: 30/03/2014, 16:20

... Practice for Handling Chickens from Hatchery to Slaughterhouse (1983); Recommended Code of Practice for Care and Handling of Pigs (1984); Recommended Code of Practice for the Care and Handling ... caged poultry 5.2 Facilities for and handling of loosehoused poultry 5.3 Catching and loading 5.4 Transport 29 30 30 32 Section Processing plants 34 6.1 Receiving and handling of poultry 6.2 Slaughter ... Practice for the Care and Handling of Mink (1988); Recommended Code of Practice for the Care and Handling of Ranched Fox (1989) This code is an updated version of the 1983 publication on the care and...

Ngày tải lên: 31/03/2014, 08:20

... with elevation drawings, plant- layout arrangements, and other information required for the actual construction of the plant The final stage * I _ PLANT DESIGN AND ECONOMICS FOR CHEMICAL ENGINEERS ... Provisions for possible shutdowns for repairs and maintenance are incorporated into the design of the process by I 26 PLANT DESIGN AND ECONOMICS FOR CHEMICAL ENGINEERS specifying plant operation for ... costs for raw materials, labor, maintenance, power, and utilities must all be included along with costs for plant and administrative overhead, distribution of the final products, and other miscellaneous...

Ngày tải lên: 01/04/2014, 11:35

... electrodes(Fig 3) are most frequently used for electroporation They are available with different gap sizes,typically mm (for bacteria), and and mm (for mammalian cells and plant protoplasts) The electric ... implications for medical therapies for electrical shock injury, and may also help us to understand the mechanism by which membranes recover 24 Cell Stress and Viability Complete cell viability, ... volumetric ratio: ~“01 = (~extracellular/~~ntracellular) (9) may correlate with cell death or survival (47) According to this hypothesis, for a given cell type and extracellular medium composition,...

Ngày tải lên: 11/04/2014, 10:10

Ngày tải lên: 12/05/2014, 15:38

... cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C for 10 mins ... conditions consisted of 30 cycles of 94°C for min, 58°C for and 72°C for mins JSP001 &JSP002: PCR conditions consisted of 30 cycles of 94°C for min, 45°C for and 72°C for mins Uni F &Uni R: K = G + T, ... 94°C for 30 secs., 60°C for and 72°C for 1.5 mins followed and extension of mins at 72°C AC1F &AC1R : PCR conditions consisted 94°C of mins followed by 35 cycles of 94°C for 30 secs, 58°C for and...

Ngày tải lên: 19/06/2014, 08:20

... cycles of 94°C for min., 58°C for and 72°C for MSV F &MSVR : PCR conditions consisted of 30 cycles of 94°C for min, 59°C for and 72°C for mins C1F &C2R: PCR conditions consisted of 94°C for 10 mins ... conditions consisted of 30 cycles of 94°C for min, 58°C for and 72°C for mins JSP001 &JSP002: PCR conditions consisted of 30 cycles of 94°C for min, 45°C for and 72°C for mins Uni F &Uni R: K = G + T, ... 94°C for 30 secs., 60°C for and 72°C for 1.5 mins followed and extension of mins at 72°C AC1F &AC1R : PCR conditions consisted 94°C of mins followed by 35 cycles of 94°C for 30 secs, 58°C for and...

Ngày tải lên: 20/06/2014, 04:20

... service o Number born, and date o Number scouring, (duration of treatment, and a record of any samples collected for NIVR) o Age and speculative cause of death o Number weaned and date o Date of ... farmers to improve their production and therefore their bottom line Our aim is for our selected farmers to become community leaders and produce breeding stock for neighbouring farmers (highly lucrative) ... the flooring is removable for cleaning and there are two openings and a light bulb, which provides warmth Straw is commonly added as bedding • Group housing in pens for dry sows with feeding...

Ngày tải lên: 21/06/2014, 06:20

... favan-3-ol and are found in apples, grapes, strawberries, plums, sorghum and barley (Davidson, 1997) 14 Fungicides for Plant and Animal Diseases Benzoic acid, proanthocyanidins and flavonols account for ... are abundant in the soil adjacent to plant roots (rhizosphere) and within healthy plant tissue (endophytic) and a proportion possess plant growth promotion and disease resistance properties Actinobacteria ... antifungal agents 30 Fungicides for Plant and Animal Diseases that degrade cell walls and inhibit the synthesis of mannan and β-glucan enzymes, antiparasitic agents and insecticidal agents Actinobacteria...

Ngày tải lên: 22/06/2014, 03:20

... 52 Fungicides for Plant and Animal Diseases Oki, T., Tenmyo, O., Hirano, M., Tomatsu, K and Kamei, Hl, 1990b Pradimicins A, B and C: New antifungal antibiotics II In vitro and in vivo biological ... al., 1995b and 2003a) The hydrophobicity of 56 Fungicides for Plant and Animal Diseases molecules is often associated with biological action (Hansch and Dunn, 1972) However, the rationale for this ... analogues are common in many plants (Kim et al., 1995; Kubo and Kubo, 1995; Kubo et al., 1996 and 1999; Kubo and Fujita, 2001; Trombetta et al., 2002) and readily available Therefore, a homologous series...

Ngày tải lên: 22/06/2014, 03:20

... 2010, 94 Fungicides for Plant and Animal Diseases Plant Specie Larrea tridentata Laurus nobilis Metasequoia glyptostroboides Piper longum Plant pathogen Aspergillus flavus and Aspergillus parasiticus ... Battinelli et al., 2006 Bandara et al., 1992 Miles et al., 1991 Cole et al., 1991 Miles et al., 1993 Rahman et al., 1995 96 Fungicides for Plant and Animal Diseases Plant Specie Plant pathogen Fungicidal ... Agrispon TM Plant and mineral extacts Agric Sci Dallas Cercospora beticola Sincocin TM Plant extracts Agric Sci Dallas Cercospora beticola 98 Commercial name Fungicides for Plant and Animal Diseases...

Ngày tải lên: 22/06/2014, 03:20