suicide or murder 222

... is also a component of the ‘erythroid regulator’ and ‘hypoxia regulator’ Hypoxia regulator Stores regulator Erythroid regulator Inflammatory regulator Figure 2-7 The role of hepcidin in the regulation ... extracellular transport of iron, especially in abnormal conditions such as iron overload, hemolytic states, and inflammatory conditions These proteins transport iron to the liver for storage or recycling ... depends on two main factors, the ‘stores regulator’ which responds to the body’s iron store, and the ‘erythropoietic regulator’ which responds to the body’s requirement for erythropoiesis (4) An...

Ngày tải lên: 16/09/2015, 15:55

... Peptidyl-prolyl-cis-trans isomerase B precursor Fragment of lamin A or C Lamin A Lamin C or lamin A fragment Fragment of lamin A or C Fragment of lamin A or C Fragment of lamin A or C ATP binding cassette, subfamily ... care, housing and killing were in accordance with the guidelines of the Ministry of Education, Science, Sports and Culture of Japan for the use of laboratory animals Cell fractionation Subcellular ... in 35 mm dishes for 48 h, fixed with 4% paraformaldehyde for 20 and treated with 0.1% (v ⁄ v) Triton X-100 for The fixed cells were incubated with affinity-purified anti-ISP36 sera or preimmune IgG...

Ngày tải lên: 30/03/2014, 20:20

... set at 1.00 for forward scattering; the detector voltage was set to E00 for forward scattering and 600 V for FL1, and the threshold for forward scattering was set at 52 The EGFP fluorescence signal ... were set in linear mode for forward scattering and in logarithmic mode for the green fluorescence detector (FL1, 530 ⁄ 30 nm bandpass filter) and the orange fluorescence detector (FL2, 585 ⁄ 21 nm bandpass ... not show EGFP reporter fluorescence even after the addition of pheromone (Fig 3B) The fluorescence intensity for BWG2118 appeared similar to that for strain BY4741, which was the original BWG2118...

Ngày tải lên: 16/03/2014, 01:20

... We thank Dr Da-zhi Wang (University of North Carolina) for helpful discussions and comments on the manuscript This work was supported by the Program for New Century Excellent Talents in University ... 1568–1578 ª 2008 The Authors Journal compilation ª 2008 FEBS B Zheng et al hhLIM binds to F-actin reported to activate brain natriuretic factor (BNP) and atrial natriuretic factor (ANF) gene expression ... antibody against hhLIM or actin, with visualization by secondary antibodies and enhanced chemiluminescence [29,30] Fluorescence staining Fluorescence staining was performed as described previously...

Ngày tải lên: 16/03/2014, 06:20

... pJ69–4A harboring pPC97–p76RBE–HR1 or the complete p76RBE were transformed with the different Rho constructs in pPC86 For the screening, pJ69–4A harboring pPC97– p76RBE–HR1 was transformed with ... receptor on its way to lysosomes for degradation [51] and is thought to be involved in the sorting of internalized receptors for degradation [7] However, nothing is known about the molecular actors ... specificity of association of p76RBE with the activated form of RhoB suggested that it could act as a GAP protein or as a RhoB effector or inhibitor Because p76RBE does not contain the Rho GAP consensus...

Ngày tải lên: 23/03/2014, 21:20

... cardiovascular disease, neurological disorders and tumorigenesis Acknowledgements We are grateful for the support of Prof P Corvol at the College de France (INSERM) for his support with laser confocal microscopy ... required for the fluorescence signal to exceed the detection threshold value The fold difference (F) was calculated and normalized to the levels for the established HKG, b-actin, according the ... with normoxia or hypoxia) using the Superscript II RT kit (Invitrogen) and an anchored biotinylated oligodT25 cDNAs were then treated with RNAse I followed by proteinase K and phenol/chloroform...

Ngày tải lên: 30/03/2014, 14:20

... osteogenesis transcription factor is of major importance In fact, the RUNX2 pathway has been reported as a potent inhibitor of the Page of 13 expression of the master gene for adipogenesis, PPARg [26] ... for sample normalization Results are log2-transformed fold changes of normalized 2-deltaCT Data were obtained from three independent experiments (error bars represent average ± standard error) ... for sample normalization Results are log2-transformed fold changes of normalized 2-deltaCT Data were obtained from three independent experiments (error bars represent average ± standard error)...

Ngày tải lên: 09/08/2014, 23:20

... OF SINGAPORE 2010 Acknowledgements I would like to express my gratitude to my supervisor, Professor Hong Wan Jin for his supervision, guidance and support to my work; and to my supervisory committee ... sorting control for fine regulation 1.1.2.4 Post-TGN carrier formation Secretory cargos are enriched in tubular TGN subdomains through segregation by either sorting motif recognition or favored ... vesicles， dense core granules, dense core secretory granules, or large dense core vesicles Secretory granule (SG) buds from the TGN as immature SG (ISG), then undergo homotypic fusion and /or membrane...

Ngày tải lên: 11/09/2015, 09:15

... 1940s, Cori and his colleagues showed that the enzyme glycogen phosphorylase exists in two forms: an inactive form called phosphorylase b and an active form termed phosphorylase a These two forms ... receptors, G-protein-linked receptors, and enzyme-linked receptors1 1.1.2.1.1.1 Ion-channel-linked receptors Ion-channel-linked receptors are involved in synaptic signaling The receptors for some ... ligands After the binding of hormones to the receptors, the inhibitory protein complexes bound to the inactive receptors dissociate and the receptors bound to coactivator proteins induce gene transcription11...

Ngày tải lên: 12/09/2015, 21:10

... are necessary for normal otolith growth and their correct anchoring onto the sensory maculae Mech Dev 122, 791–803 24 Pisam M, Jammet C & Laurent D (2002) First steps of otolith formation of the ... responsible for the content or functionality of any supplementary materials supplied by the authors Any queries (other than missing material) should be directed to the corresponding author for the ... observed by gel electrophoresis These substances may be aggregates of proteins and polysaccharides, and may play important roles in formation of the phases and ⁄ or morphologies of the crystals...

Ngày tải lên: 18/02/2014, 17:20

... provides supporting information supplied by the authors Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset Technical support issues ... protein–protein interactions by incorporating a novel signal amplification circuit As Pheromone (α-factor) Yeast membrane Yeast membrane α Receptor Effector X α Receptor GTP γ β Y γ Protein-protein ... the family of hexose transporters Mol Cell Biol 11, 3804– 3813 Supporting information The following supplementary material is available: Doc S1 Supporting information for Materials and methods;...

Ngày tải lên: 05/03/2014, 23:20

... T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York 29 Augustin J & Gotz F (1990) Transformation of Staphylococcus epidermidis ... soy agar (Oxoid, Cambridge, UK) or trypticase soy broth at 37 °C A domain of fibronectin-binding protein B for liquid cultures Chloramphenicol (10 lgÆmL)1) was incorporated into trypticase soy broth ... (uppermost traces) protein were passed over the surface of the same Fg (A) or Fn (B) chip The sensorgrams have been corrected for the response obtained when recombinant FnBPB proteins were passed over...

Ngày tải lên: 06/03/2014, 00:20

... obtain the corresponding Dras2 (AR3) and Dsut2 (AR4) reporter strains by standard genetic methods High copy suppressor screen The Yep351-based yeast genomic library used for the suppressor screen ... Germany) The plasmid was used to transform MB1 Dras2 ura3 prior to disruption of GPA2 in order to reduce the appearance of spontaneous second site suppressors after the disruption of GPA2 GPA2 ... indicated in Fig and the storage carbohydrate levels were determined as described by Lillie and Pringle [16] Culture conditions for immunoblots were as described for storage carbohydrate determination...

Ngày tải lên: 07/03/2014, 15:20

... phenylmethanesulfonyl fluoride] and stored at )80 °C overnight The pellet was thawed on ice for h and then 10 lgÆmL)1 DNase and 20 mM MgSO4 (final concentration) were added to the cell suspension for 30 Cells ... performed at 20 °C with filtered (Millex syringe filters, 0.22 lm; Millipore Corp.) protein samples (12 lL at mgÆmL)1) using a Dynapro MSTC-200 (Protein Solutions) All calculations were performed ... Renaud Vincentelli for their technical assistance This work was supported by a grant from the 5th PCRDT program of the European Union (acronym X-TB) and by the French national network of Genopole...

Ngày tải lên: 07/03/2014, 16:20

... 48 h, cells were formaldehyde fixed, mounted in Vectashield containing 4,6-diamino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA, USA), and studied by standard fluorescence microscopy ... Set1 complex, which contains CGBP and trithorax homologues [10] The members of the trithorax group encompass various subclasses of gene regulatory factors [11]; one subclass involves chromatin ... sequence was incorporated into profiles to improve profile quality Profile-based sequence searches were performed against the nonredundant and Uniref90 protein databases with the corresponding global...

Ngày tải lên: 07/03/2014, 21:20

... targeted breakpoints for DNA sequencing Gene 28, 351– 359 Sambrook, J.T., Fritsch, E.F & Maniatis, T (1989) Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory, New York, USA Herget, ... Additionally, the finding that many tumor cell lines have no or reduced MARCKS expression supports the hypothesis that MARCKS is a tumor and /or growth suppressor gene in some cell types [25–27] The ... neural precursor cells, colocalizes with PKCg and binds calmodulin Eur J Neurosci 11, 503–516 46 Harlow, E & Lane, D (1988) Antibodies – A Laboratory Manual Cold Spring Harbor Laboratory Press,...

Ngày tải lên: 08/03/2014, 08:20

... information supplied by the authors Such materials are peer-reviewed and may be re-organized for online delivery, but are not copy-edited or typeset Technical support issues arising from supporting ... disordered To extend this finding, we also collected 74 scaffold proteins (also including proteins denoted as anchor and docking) and examined them by three different disorder predictor algorithms, ... of complex-forming proteins of related function, also including anchor and docking proteins The prototype for anchor proteins is the FEBS Journal 276 (2009) 3744–3756 ª 2009 The Authors Journal...

Ngày tải lên: 16/03/2014, 02:20

... amounts adsorbed to the glass and formaldehyde resin surfaces in at 25 °C were estimated, and the Table Amount of adsorption of rMrcp-19k to several material surfaces The adsorbed amount in ASW or dilute ... extracted from the basal or upper portion of the adult barnacle was electrophoresed in formaldehyde gel, transferred to a nylon membrane, and hybridized with a probe The basal portion mainly comprises ... science, e.g development of adsorbents for various material surfaces, of support for protein alignment on a solid surface [25–27], and of underwater adhesives for surgical use [6] Experimental...

Ngày tải lên: 16/03/2014, 05:20

... Ms H Yoshida for subcloning of HEK293 cells and Dr K Hitomi for valuable suggestions This work was supported by a Grant-in-Aid for Scientific Research B (to M Maki), a Grant-in-Aid for Young Scientists ... responsible for the content or functionality of any supplementary materials supplied by the authors Any queries (other than missing material) should be directed to the corresponding author for the ... Biosci 9, 1817–1832 Odorizzi G (2006) The multiple personalities of Alix J Cell Sci 119, 3025–3032 Sadoul R (2006) Do Alix and ALG-2 really control endosomes for better or for worse? Biol Cell 98,...

Ngày tải lên: 16/03/2014, 06:20