studying vascular progenitor cells in a neonatal mouse model

... PUBLICATION AND PRESENTATIONS Characterization of human umbilical cord lining derived epithelial cells and transplantation potential Zhou Y, Gan S U, Lin G, Lim Y T, Masilamani J, Mustafa F, Phua ... epithelial cell AF amniotic fluid AFSC amniotic fluid stem cell AHU animal holding unit AMC amniotic mesenchymal cell APC allophycocyanin balb bagg albino BLAST basic local alignment search tool ... the increased risk of cardiovascular disease Other complications such as chronic nephropathy, retinal damage and neuropathy, lead to conditions as severe as kidney failure, blindness and limb amputation...

Ngày tải lên: 11/09/2015, 10:02

... were always assayed at the same time and in the same way All samples were always determined in triplicate and in a blind fashion Immunoblot analysis An aliquot of brain homogenates was electrophoresed ... outlined anatomical area in the image, as previously described [9,16,17] Analyses were always performed in a coded fashion Statistical analysis Data are expressed as mean ± standard error of mean ... this AD-like brain A amyloidosis model Previously, we have shown that a full dose of indomethacin alone despite a significant reduction in brain inflammation had only a marginal effect on brain...

Ngày tải lên: 19/06/2014, 22:20

... Zealand (Masoli et al., 2004) In developing countries, asthma prevalence is increasing sharply with the progress of urbanization The increase in China and India will lead to a dramatic increase in the ... the lung parenchyma has significant limitations Characteristic features airway remodelling of human asthma such as intraepithelial recruitment of eosinophils, lamina propria inflammation and subepithelial ... in already sensitized animal results in an attenuation and even complete abrogation instead of an increase or maintenance of airway inflammation (Akbari et al., 2001) Therefore, it is imperative...

Ngày tải lên: 10/09/2015, 09:04

... (1997) alpha-Galactosidase A deficient mice: a model of Fabry disease Proc Natl Acad Sci USA 94, 2540–2544 Chiba Y, Sakuraba H, Kotani M, Kase R, Kobayashi K, Takeuchi M, Ogasawara S, Maruyama Y, Nakajima ... Fabry mouse; (C, D) lung – epithelial cell staining increased in Fabry mouse; (E, F) brain microvascular endothelial staining in Fabry mouse Magnification ·16 no VT1 staining of normal brain (Fig ... 7F) In Fabry brain, extensive staining of the microvasculature is evident (Fig 7E) The arachnoid membrane surrounding the brain is extensively stained in Fabry but not normal mouse brain Although...

Ngày tải lên: 19/02/2014, 07:20

... macrophages, myofi- Capillaria hepatica infection in a rat liver 213 broblasts and mast cells were increased in animals with hepatic fibrosis and these cells might play a role in the formation ... Souza MM, Silva LM, Barbosa AA Jr, de Oliveira IR, Parana R, Andrade ZA Hepatic capillariasis in rats: a new model for testing antifibrotic drugs Braz J Med Biol Res 2000, 33, 1329-1334 Ferreira ... that a similar mechanism involving macrophages, myo-fibroblasts and mast cells might be associated with the septal fibrosis in this rat infected with C hepatica Moreover, by double staining via...

Ngày tải lên: 07/08/2014, 20:23

... of myelinated areas in damaged spinal cords were calculated using the formula, (myelinated areas/total area) × 100, from images of the transverse sections using image analyzer software (ImageJ ... propofol (Ha Na Pharm, Korea) at mg/kg Anesthesia was maintained by 2% isoflurane (Ilisung, Korea) in oxygen The minimum alveolar concentration was about 1.5 A multiparameter anesthetic monitor (Datex-Ohmeda, ... morphine at 0.3 mg/kg The dogs were induced with IV administration of propofol (Ha Na Pharm, Korea) at mg/kg After tracheal intubation, anesthesia was maintained by isoflurane (Ilisung, Korea) in...

Ngày tải lên: 07/08/2014, 23:22

... 5'-aagcaaacctcggggatact-3' and anti-sense 5'-ggggcttgatctcaaaat ga-3'; and beta microglobulin, sense 5'-gggttt catccatccgac-3' and anti-sense 5'-acggacggcatactcatc-3' For protein detection of caspase ... H, Hayashi K, Watanabe T, Harada M: Characterization of caspase-8L: a novel isoform of caspase-8 that behaves as an inhibitor of the caspase cascade Blood 2002, 99:4070-4078 40 Mohr A, Zwacka RM, ... death effector domain (DED), it lacks the carboxy-terminal half of the proteolytic domain Caspase 8L binds to the Fas-associating protein with death domain (FADD) and blocks the binding of caspase...

Ngày tải lên: 09/08/2014, 10:20

... (g) Apgar Score < (5 min) Asphyxia RDS IUGR Mean of variables Mean PR (/min) Mean RR (/min) Mean SpO2 (%) Treatment of data sampling Oxygenation Intubation Aminophylline Phenobarbital Midazolam ... addition, an amplitude index was calculated as follows: Amplitude index = amplitude ÷ mean of variables × 100 Statistical analysis Data were analyzed with SPSS and Statview ANOVA was used to evaluate ... Maintained in a Neonatal Care Unit Sleep Research Online 2001, 4(3):77-82 Schimmel M, Waterhouse J, Marques MD, Weinert D: Circadian and Ultradian Rhythmicities in very premature neonates Maintained...

Ngày tải lên: 10/08/2014, 09:20

... histological examination tumors were explanted, fixed in 4% formalin and embedded in paraffin Hematoxylin/Eosin staining of slices was performed according to standard protocols All animal protocols ... of Gd-BOPTA was increased according to dosage applied in men (0.1 mmol/kg) Animals were anaesthetised via i.p application of ketamine/xylazine mixture prior to imaging Body weight was assessed ... GNU Image Manipulation Program (GIMP 2.6.8) and calculating the area using formula A = a/ 2 × b/2 × π Results Imaging of organs and tumors; gadobenate dimeglumine (Gd-BOPTA) induced MRI contrast...

Ngày tải lên: 10/08/2014, 10:21

... abdominal cavity Intravenous and intrathoracic inoculation of immune cells causes accumulation of effector cells in the thoracic cavity, but not in the abdominal cavity Intra-abdominal inoculation of ... had already been confirmed against autologous tumor cells Cytological examination of the ascites before and after treatment was carried out using Papanicolaou stain We also examined quantitatively ... symptomatic treatments Abdominocentesis and drainage alleviate symptoms caused by ascites such as abdominal distention, dyspnea and oliguria, but cause hypovolemia, hypoproteinemia and general malaise...

Ngày tải lên: 11/08/2014, 19:21

... for aSMA days after transplantation (B) Instead, EGFP+/prominin-1+ cells transplanted days after bleomycin treatment (into the lung with ongoing inflammation) and analyzed at day 21 were negative ... immunofluorescence analysis revealed increased amount of cells with non-polarized prominin-1 localization on the cellular membrane during the inflammatory phase (d7) and accumulation of aSMA+ myofibroblasts ... of prominin-1+ progenitors during acute pulmonary inflammation We injected intratracheally bleomycin and, after days, sorted prominin-1 + cells from the inflamed lung and expanded them in the CEM...

Ngày tải lên: 12/08/2014, 14:20

... matrix degrading enzymes (3) such as MMPs and aggrecanases These events promote cartilage degradation and stimulate further joint inflammation (4) and a self-perpetuating inflammatory and catabolic ... longa is a promising therapeutic agent for the treatment of OA and RA as it has pro-apoptotic properties in synovial lining cells [33,34] and has been shown to have anti-inflammatory and anti-apoptotic ... treatment of OA/RA and for OA/RA cases where cartilage damage is marginal Abbreviations AP30 3a: alkaline phosphatase linked sheep anti -mouse; AP30 4A: sheep antirabbit secondary antibodies; DMSO: dimethylsulfoxide;...

Ngày tải lên: 12/08/2014, 14:22

... donor cells into alveolar air space Migration of labeled donor cells into alveolar air space In pneumonia lungs, the ratio of cells that migrated into alveolar air space was measured The results are ... Harbor, ME) and maintained on a standard laboratory diet and housed in a controlled environment with a 12-hour light/dark cycle in the animal care facility at Jack Bell Research Centre All animal experiments ... chemo-attractants in the acute inflammatory milieu but are recruited later when cells for resolution and repair are required Alternatively, these cells may remain in the less hostile intravascular...

Ngày tải lên: 12/08/2014, 15:21

... CD166 + cells in FACS and immunohistochemical staining in individual samples were analyzed by using the one-tailed Spearman rank test (since a negative correlation between the parameters was not ... all normal-appearing tissue was harvested To standardize the procedure and to avoid contamination of the chondrocytes with bone marrow cells, the subchondral lamella was left intact in all cases ... potential in human articular cartilage In addition, the percentage of MPCs in OA and normal cartilage is substantially higher than previously reported, indicating a yet unexplored reserve capacity...

Ngày tải lên: 12/08/2014, 15:23

... domain C1 EF hands domain CDC25 box * * variant (581AA) * variant (604AA) variant (484AA) * 7, 8, * 14 int 15 * 7, * variant 11 (642AA) * variant 12 (454AA) 11 int 12 variant 13 (669AA) 12 variant ... Variant Variant Variant FL Variant Variant FL FL Variant Variant Variant Variant Variant DAS28: The Disease Activity Score in 28 joints; FL: full-length; IFX: infliximab; MTX: methotrexate; PSL: ... Reverse Transcription kits (Qiagen) The coding regions of the hRasGRP4 cDNAs were then amplified by a PCR method using the forward 5'-AGCATGAACAGAAAAGACAGTAAG-3' and the reverse 5'-TGTCTAGGAATCCGGCTTGGA-3'...

Ngày tải lên: 12/08/2014, 18:20

Ngày tải lên: 12/08/2014, 23:21

... premedication, general anesthesia was induced by intravenous injection of mg/kg etomidate (Braun) Anesthesia was maintained by continuous intravenous injection administration of ketamin hydrochloride ... Characterization of endothelial progenitor cells The attached spindle cells at different points were all positive for taking up Dil-ac-LDL and UEA-1 Co-staining cells revealed that more than ... (serum alanine aminotransferase, serum aspartate aminotransferase or total bilirubin more than two times that of normal), and renal dysfunction (creatinine or blood urea nitrogen more than two times...

Ngày tải lên: 13/08/2014, 18:22

... not part of the training data for official MUC-6 evaluation) This model gave 68.0 F1 and is labeled +DRYRUN-TRAIN in table 2 (a) We then added the ACE ENGLISH-NWIRE training data, which is from a ... measure Vilain et al (1995) The table in (a) is our performance on the thirty document MUC-6 formal test set with increasing amounts of training data In all cases for the table, we are evaluating on ... JMLR Pascal Denis and Jason Baldridge 2007 Global, joint determination of anaphoricity and coreference resolution using integer programming HLT-NAACL Thomas Ferguson 1973 A bayesian analysis...

Ngày tải lên: 08/03/2014, 02:21

Ngày tải lên: 15/03/2014, 14:20

... relevant features (such as L-marking, Case, and 0) If we adhere to the representational paradigm used above, we can define Chains in the following manner: Chain Schevaa Node: C-Node: {Cat,Level,Pos,ID,Ftrs} ... a particular representation provides a formal characterisation of locality Just as phrase structure is defined in terms of branches, we can define Chains as a sequence of links More specifically, ... structures are limited to some combination of binary (non-terminal) and unary (terminal) branches As discussed above, we can characterise the representational framework in terms of nodes and schemas:...

Ngày tải lên: 18/03/2014, 02:20