studies on fine structure and function of synapses

... cultures on H2O2 formation and on cell survival in Figure catalase LSEC of9 Effect of catalase on H2O2 formation and on cell survival in LSEC cultures Formation of H2O2 was measured on LSEC cultures ... Significant con- http://www.comparative-hepatology.com/content/7/1/4 23 24 25 tribution of liver non-parenchymal cells to metabolism of ammonia and lactate, and cocultivation augments the functions of ... expression on cultured LSECs ICAM expression on cultured LSECs Quantitative measurements of ICAM-1 expressed on the surface of LSEC were done by flow cytometry after incubation of LSECs on normoxic and...

Ngày tải lên: 13/08/2014, 13:20

... actions were observed, depending on the concentration The addition of DiS-C3(5) £ 10 lM caused deceleration of mitochondrial oxygen consumption but > 20 lM caused acceleration These actions of ... spontaneous acceleration of mitochondrial Ó FEBS 2004 Effects of DiS-C on mitochondrial structure and function (Eur J Biochem 271) 3577 Fig Inhibitory effects of a low concentration DiS-C3(5) on the Ca2+-induced ... Effects of DiS-C3(5) on the turbidity of mitochondrial suspensions (A) and on permeability of mitochondrial inner membrane (B) (A) The effect of 50 lM DiS-C3(5) on the turbidity of mitochondrial...

Ngày tải lên: 16/03/2014, 18:20

... Objective of this study 6.6 Methods 6.6.1 Expression, purification and characterization of Nm and Ng 6.6.2 Cloning, expression and purification of CaM constructs 6.6.3 Isothermal titration calorimetry ... gain structure upon binding with its partners Chapter provides the conclusion and future direction for part II of this thesis ix List of Tables Page Table 2.1: Crystallization conditions and data ... activity of several enzymes by Ca2+ signal, undergoes N-methylation as one of the several posttranslational modifications (Cobb and Roberts 2000) Methylation alters the function of CaM in vivo, and...

Ngày tải lên: 10/09/2015, 08:38

... premature initiation of transcription elongation [137] The initiation and elongating phases of the transcription cycle are characterized by distinct sets of histone and Pol II modifications [19,151] ... enzymatic functions of PARP-1 are activated in the presence of NAD+ it mediates the PARylation of both histones and PARP-1 itself, and thereby promotes decondensation of higher order chromatin structure ... histone acetylation and deacetyla2192 tion, and transient recruitment of remodellers and transcription factors A cycle of transcription commences with the recruitment of transcription factors and...

Ngày tải lên: 14/02/2014, 18:20

... deletions of the CD2-1 region (Fig 3E) and examined the interactions between bacterially expressed proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the KIND family of proteins, only ... v-KIND and MAP2 contributes to the interaction between the two proteins In conclusion, the present study has clarified the structural and functional importance of the v-KIND and MAP2 interaction core ... with lysis buffer and subjected to immunoblot analysis Plasmid construction and expression Immunoblotting Plasmid construction and expression in Escherichia coli or African green monkey kidney cell...

Ngày tải lên: 14/02/2014, 19:20

... of Biological and Chemical Sciences, Queen Mary, University of London, UK DAVID G RAFFAELLI Environment Department, University of York, UK RONNI EDMONDS-BROWN Division of Geography and Environmental ... set of adaptive responses to information about environmental conditions and physiological state Thus a theory of reaction norms has to deal with two effects of the environment on a reaction norm ... rates of metabolism and ontogenetic growth are limited by whole-body concentrations of RNA Not only does the C:P ratio explain most of the residual variation in development rates as a function of...

Ngày tải lên: 17/02/2014, 19:20

... intron sizes, the typical and nucellinlike Arabidopsis APs exhibit conservation of exon/intron organization when compared to the coding region of AP genes from Brassica, rice [7,8,55,86] and ... atypical and their localizations are unexpected New questions concerning functional significance and specialization of this multigene family of proteases are now starting to be addressed and will ... Ribbon representation of the structure of the PSI domain of barley prophytepsin [25] (N-terminal domain, blue; C-terminal domain, red) (C) Model structure of the PSI domain of cardosin A based on...

Ngày tải lên: 19/02/2014, 12:20

... form functional channels in any pairwise combination [13–15] From analysis of single channel conductances obtained upon coinjection of wild-type and mutant subunits, and from quantification of radiolabelled ... subtypes, and the determination of their precise subunit composition and physiological function( s) Compared to the muscle nAChR, relatively little is known about the function and composition of the ... compared to conventional venom fractionation is that only small amounts of tissue are required In addition, conotoxins with low expression levels that would escape detection in functional assays...

Ngày tải lên: 19/02/2014, 12:20

... addition of an additional one, two or three b-helical coils compared to the shorter isoforms In the case of one sbwAFP isoform, named CfAFP-501, a detailed examination of the structure and function ... AFP isoforms In addition to in vitro mutations, the comparison of isoform sequences can demonstrate which residues are important for a protein’s function and structure A list of known isoforms ... arrangement of water on the protein surface, only INPs are large enough to support continued growth Conclusion Analysis of the structure and examination of the ice-binding behaviour and point mutants of...

Ngày tải lên: 19/02/2014, 16:20

... showed only a minor effect of temperature on the existence and line widths of the protein backbone resonances This suggests the absence of multiple conformations that interconvert on the millisecond ... translation termination The superposition of the families of solution structures of the two conformers onto the crystal structure of human eRF1 (3E1Y) gives an rmsd for the heavy pro˚ tein backbone ... Ramachandran map Percentage of residues in 0 disallowed region of the Ramachandran map Superimposition of the structures on the representative structure rmsd over backbone C, CA, O 0.42 0.42 and...

Ngày tải lên: 06/03/2014, 11:20

... identification and distribution of TLP genes in nature, the structural and functional characterization of the TLP from various organisms, and the evolution of TLP and transthyretin The identification of ... portions of strands B and C that protrude towards the solvent and in the conformation of the long loop connecting strands D and E [17] Greater differences are observed between the structures of ... in TLP structures as a result of alterations to the formation of hydrogen bonds between strands The carbonyls of residues V104 and P105 (zebrafish TLP numbering), in the middle of b-strand G,...

Ngày tải lên: 07/03/2014, 00:20

... Valverde et al Structure and function of KH domains A B C Fig 10 Crystal structure of tandem type II KH domains of NusA in complex with RNA The tandem KH1–KH2 domains of NusA recognize RNA ligand 5¢-GAACUCAAUAG ... poly(C)-rich DNA and RNA sequences and function in a diverse range of cellular processes, including mRNA stabilization, translational activation, and translational silencing [31,32] Crystal structures ... crystal structure of tandem type II KH domains NusA regulates transcriptional elongation, pausing, termination and antitermination in prokaryotes [39– 2718 41] The protein contains two tandem type...

Ngày tải lên: 07/03/2014, 05:20

... positions of the cysteines of GlcNAc kinase are displayed in Fig 5B Cysteines C211 and C45, whose Fig Predicted three-dimensional structure of GlcNAc kinase (A) Front view of the threedimensional structure ... III, a-helix (B) Top view of the threedimensional structure of GlcNAc kinase The relative positions of cysteines are labeled with a star Ó FEBS 2002 Structure and function of GlcNAc kinase (Eur ... & Sander, C (1993) Prediction of protein secondary structure at better than 70% accuracy J Mol Biol 232, 584–599 19 Jones, D.T (1999) Protein secondary structure prediction based on position-specific...

Ngày tải lên: 08/03/2014, 10:20

... Meade, G., Foley, V.M & Dowd, C.A (2001) Structure, function and evolution of glutathione transferases: implications for classification of non-mammalian members of an ancient enzyme superfamily Biochem ... Expression and purification of maize GST I and other enzymes Maize GST I was cloned into a pQE70 expression vector to yield the pQEGST expression plasmid as described by Labrou et al [14] Expression and ... concentration of SDTG, k3 is the maximal rate of inactivation (min)1), and Kd is the apparent dissociation constant of the E:SDTG complex From the data shown in Fig 2, Kd values of 107.9 ± 2.1 lM and...

Ngày tải lên: 16/03/2014, 16:20

... sulfuric acid concentration at the same voltage Oxide growth rate and its rate of dissolution partially depended upon concentration of sulfuric acid Higher electrolyte concentration increased oxide ... dynamic equilibrium of the oxidation and dissolution rates It is proposed that the attenuation of the oxidation rate and the resulting limiting thickness is due to depletion of oxygen ions in the acid ... The dynamic equilibrium of the oxide formation (highly dependent on the acid concentration and availability of oxygen ions) and dissolution (relatively constant due to its field-assistant nature)...

Ngày tải lên: 19/03/2014, 16:48

... intentionally left blank Studies on the Ecology and Conservation of Butterflies in Europe Studies on the Ecology and Conservation of Butterflies in Europe Vol 1: General Concepts and Case Studies ... Studies on the Ecology and Conservation of Butterflies in Europe STUDIES ON THE ECOLOGY AND CONSERVATION OF BUTTERFLIES IN EUROPE Vol 1: General Concepts and Case Studies Edited ... &Butterflies(Eds) 2005 of Maculinea butterflies in Europe 15 Studies on the Ecology and Conservation of Vol 1: General Concepts and Case Studies, p 15 Consequences of the spatial configuration of resources...

Ngày tải lên: 23/03/2014, 01:20

... a-crystallin domain of human sHsp is very conservative and the loop connecting the b5 and b7 strands is shorter than the corresponding loop connecting the b5 and b7 strands of nonmetazoan sHsp (Fig ... insulin results in dissociation of its peptide chains and aggregation of chain B Addition of the wild-type HSP22 retarded the onset of aggregation and decreased the amplitude of light scattering induced ... overall structures of aB-crystallin and HSP27 predicted by these two models are very similar, and the positions of the b-strands correlate well with the corresponding positions of the b-strands...

Ngày tải lên: 23/03/2014, 07:20

... CD studies not show any change on the secondary structure of blg-A upon interaction with concentrations of 15.54 and 35.8 mm of Pb2+, while far CD studies show considerable change in the secondary ... make use of conformational mobility (i.e hindered rotation around chemical bonds as well as molecular construction deformations with formation and breakage of non-valent bonds) Components of molecular ... sodium-ion driven components that can function well with sodium ions in Escherichia coli We measure the dependence of the motor speed and internal sodium concentration on external sodium concentration...

Ngày tải lên: 23/03/2014, 15:20

... way of resolving the enigma arising from the ‘one gene – one protein – one function argument, and a new mechanism of diversifying protein function Thus, in addition to alternative splicing of ... the cofactor as oleic acid and the conditions required for complex formation were defined by deliberate conversion of native a-lactalbumin to an active complex on an ion exchange column conditioned ... Titration of apo human a-lactalbumin with oleic acid accompanied by determination of the critical micelle concentration of oleic acid has also resulted in the formation of complexes with Structure and...

Ngày tải lên: 29/03/2014, 21:20

... Kajander T, Lehtio L, Schlomann M & Goldman A ¨ ¨ (2003) The structure of Pseudomonas P51 Cl-muconate lactonizing enzyme: co-evolution of structure and dynamics with the dehalogenation function ... JM109(pSHCMC1S2) converted 3CM to 4-carboxymuconolactone, and a CMLE activity of 9.4 UÆmg)1 of protein was determined In contrast, no conversion of 3CM was found in cell extracts of E coli JM109, ... (rmsd of 1.6 A for 1192 Ca atoms of a tetramer and 1.44 for 306 Ca atoms of a monomer) This indicates that not only is the monomer structure conserved, but also the quaternary structure of the...

Ngày tải lên: 30/03/2014, 10:20