studies in mechanobiology tissue engineering and biomaterials volume 2

... Cell Res 1998, 23 8 :26 5 -27 2 Martin I, Jakob M, Schafer D, Dick W, Spagnoli G, Heberer M: Quantitative analysis of gene expression in human articular 21 22 23 24 25 26 27 28 29 30 31 32 cartilage ... 173° Prim OA 4.5 2, 054 1, 923 11* 1,310 1,938 1.5 3,465 3,091 9* 2, 649 6.5 184° 2, 917 1.5 Yes 2, 147 5.5 V n/a Female 2, 422 n/a 83 2, 9 12 n/a Unaffected n/a n/a 2, 410 An asterisk indicates a significant ... participated in the design of the study and gave clinical cell culturing input LC isolated the biopsies and gave clinical feedback LP and MB provided critical clinical input to the study design and to...

Ngày tải lên: 09/08/2014, 06:22

... (m, 22 H), 0. 92 (t, 3H); 13C NMR (300 MHz, C6D6) δ 154.8, 94.6, 85.6, 80 .2, 79.7, 79 .2, 65.0, 64 .2, 63.8, 63 .2, 62. 8, 61.9, 32. 0, 29 .8, 29 .6, 29 .4, 29 .2, 28 .9, 28 .7, 28 .0, 25 .8, 25 .4, 23 .2, 22 .7, ... SPHK Inhibitors 3. 72 (m, 2H), 2. 18 (t, 2H), 1.43 (s, 9H), 1 .24 (m, 12H), 0.85 (t, 3H); 13C NMR (300 MHz, CDCl3) δ 156 .2, 87.9, 80.0, 64.3, 62. 6, 55.8, 31.7, 29 .1, 29 .0, 28 .8, 28 .4, 28 .2, 22 .5, ... 64.44% 72. 98% 63.88% Structures of six obtained compounds (analogues of sphingosine) 2. 2 .2 Compounds Inhibitory Function on SPHK Activity in vitro 2. 2 .2. 1 Over-expression of SPHK1 and SPHK2 2. 2 .2. 1.1...

Ngày tải lên: 11/09/2015, 16:06

... 28 days vs OD 28 days, P

Ngày tải lên: 11/09/2015, 16:06

... were incubated on ice for 20 min, followed by two washes in 2% FCS/PBS, before resuspension in 100 μl of 4% paraformaldehyde and incubation on ice for 20 Cells were again washed twice in 2% FCS/PBS ... cultured in 6-well plates were washed twice with 1x PBS and starved in serum-free DMEM for half an hour 1μM S1P was then added into the wells and incubated at 37°C for 5min, 10min, 30min, and 60min ... 5μM Fura2/AM (Molecular Probes, Invitrogen Singapore) Human BM- and AD- MSCs were separated into samples of 0 .2 million cells, and were incubated at 37oC for 30 and agitated every 15 minutes Afterwards,...

Ngày tải lên: 11/09/2015, 16:06

... Conclusion and Future Work being one of the most potent and specific SPHK1 inhibitors (chapter 2) , DMS was continuously used as a reference inhibitor It is interesting to note that CP6 and DMS affected ... the use of SPHK inhibitors in preconditioning stem cells before transplanting them into the body, by committing stem cells along specific differentiation path More work, including in vivo work, ... amounts (1μM DMS and 5μM CP6) showed to inhibit the osteogenic differentiation in most of the experiments Interestingly, in human BM- and AD- MSCs adipogenic differentiation, DMS and CP6 showed...

Ngày tải lên: 11/09/2015, 16:06

... 10(19): 28 18 -22 Shu, X., W Wu, et al (20 02) "Sphingosine kinase mediates vascular endothelial growth factor-induced activation of ras and mitogen-activated protein kinases." Mol Cell Biol 22 (22 ): ... Sacket, et al (20 08) “Characterization of N, N-dimethyl-derythro-sphingosine-induced apoptosis and signaling in U937 cells: independence of sphingosine kinase inhibition.” Prostaglandins & Other ... N,N-dimethyl-Derythro-sphingosine-induced apoptosis and signaling in U937 cells: Independece of sphingosine kinase inhibition.” Prostaglandins & other lipid Mediators 86: 18 -25 Kim, Hyo-Lim, Santosh...

Ngày tải lên: 11/09/2015, 16:06

... FTY 720 P, is an agonist of all S1P receptors except S1PR2 (Brinkmann et al., 20 02; Mandala et al., 20 02; Taha et al., 20 06) By preventing S1P binding to S1PRs, FTY- 720 P induced an increase in lymphocyte ... al., 20 02; Pitson et al., 20 02) These domains may play a role in substrate recognition (Pitson et al., 20 02) Both SPHK1 and SPHK2 are capable of phosphorylating erythro-sphingosine, dihydrosphingosine ... could exert a proinflammatory effects by promoting neutrophil chemotaxis (Cummings et al., 20 02; Ibrahim et al., 20 04), and induced certain proteins Chapter Introduction important in inflammation,...

Ngày tải lên: 11/09/2015, 16:06

... direct binding to canonical binding sites in the rDNA promoter (Arabi et al 20 05; Grandori et al 20 05; Poortinga et al 20 04) rDNA genes are transcribed by polymerase I in the nucleolus, arguing that ... since Myc leads to an activation of E2F-dependent transcription and distinct activating E2F proteins act downstream of Myc in controlling cell proliferation and tumorigenesis (Baudino et al 20 03; ... Smith C, Nevins JR (20 01) Myc requires distinct E2F activities to induce S phase and apoptosis Mol Cell 8:105–113 Lindstrom MS, Wiman KG (20 03) Myc and E2F1 induce p53 through p14ARF -independent...

Ngày tải lên: 25/10/2013, 21:20

... obtained data indicating that a-defensin-1 and a-defensins -2 induce an increase in the protein content of cyclin D and that quercetin inhibits the a-defensininduced increase in cyclin D protein ... caused by a-defensin-1 and a-defensin -2 p38 mitogen-activated protein (MAP) kinase inhibitor SB203580 and phosphoinositide 3-kinase (PI3K) inhibitor LY2940 02 prevented increases in the phosphorylation ... a-defensin-1 and a-defensin -2 (2. 5 lm) in the presence and absence of quercetin (10 lm) was determined As shown in Fig 4, incubation of lung fibroblasts with a-defensin-1 and a-defensin -2 resulted in...

Ngày tải lên: 18/02/2014, 06:20

... laminin chains in bovine follicles J Reprod Fertil 1998, 1 12( 2) :26 7 -27 8 Zhao Y, Luck MR: Gene expression and protein distribution of collagen, fibronectin and laminin in bovine follicles and ... protein kinase in insulin and insulin-like growth factor I (IGF-I) signaling cascades for progesterone and IGF-binding protein-1 production in human granulosa cells J Clin Endocrinol Metab 20 03, ... alpha(4)/alpha(9) integrins, alpha(6) integrins, and CD9 in the interaction of the fertilin beta (ADAM2) disintegrin domain with the mouse egg membrane Biol Reprod 20 02, 66(4):1193- 120 2 Chen CC, Chen N, Lau...

Ngày tải lên: 05/03/2014, 17:20

... cervical cancer 14 15 16 17 18 19 20 21 22 23 24 25 benz(a)anthracene-induced skin papillomas and carcinomas by dehydroepiandrosterone and 3-b-methylandrost-5-en-17-one in mice Cancer Res 45, 164–166 ... Candas B (1998) DHEA and the intracrine formation of androgens and estrogens in peripheral target tissues: its role during aging Steroids 63, 322 – 328 Lu SF, Mo Q, Hu S, Garippa C & Simon NG (20 03) ... Counts 20 30 G1 CD 20 0 400 600 FL2-A 800 1000 20 0 400 600 FL2-A 800 1000 400 600 FL2-A 800 1000 20 0 400 600 FL2-A 800 1000 20 0 400 600 FL2-A 800 1000 Counts 20 10 0 40 40 30 30 Counts 20 Counts 20 ...

Ngày tải lên: 16/03/2014, 00:20

... protein kinase A, extracellular signal-regulated kinase ⁄ 2, and phosphatidyl inositol 3-kinase ⁄ Akt signaling Endocrinology 148, 5 12 529 Cui QL, Fogle E & Almazan G (20 06) Muscarinic acetylcholine ... 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Ba Curr Biol 7, 26 1 26 9 Fayard E, Tintignac LA, Baudry A & Hemmings BA (20 05) Protein kinase B ⁄ ... preventing PI3K activation [36] Following agonist-induced activation, dopamine D2 receptors are internalized and form a multiprotein complex that includes b-arrestin, protein phosphatase 2A and...

Ngày tải lên: 16/03/2014, 05:20

... the number of bands and the labeling intensity were increased in growing cells The most prominent increases were in bands of 140, 133, 116, 95, 82, 73 and 56 kDa (Fig 4) Other bands also showed ... Characterization of botulinum C3-catalyzed ADP-ribosylation of rho proteins and identification of 20 21 22 23 24 25 26 27 28 29 30 31 32 mammalian C3-like ADP-ribosyltransferase Mol Cell Biochem 138, ... FEBS 20 03 1 02 L Yau et al (Eur J Biochem 27 0) include various GTP-binding proteins, including Gs, Gi and Rho [13–15] Although these target proteins suggest a role for mono-ADP-ribosylation in signal...

Ngày tải lên: 17/03/2014, 09:20

... peptidases, DP8 and DP9 15 16 17 18 19 20 21 22 23 24 25 26 27 invasive activity attributable to dipeptidyl peptidase IV overexpression in ovarian carcinoma Cancer Res 62, 27 53 27 57 Wesley UV, Albino AP, ... b1-integrin [29 ], so the effects of DP8 and DP9 on p38, b1-integrin and DDR1 phosphorylation require examination Changes in TIMP2 and b-catenin expression may be secondary to effects on integrins and ... fibronectin and integrin binding [10,18 ,29 ] The increased STS-induced apoptotic effect of DP8 and DP9 may indicate that under certain biological FEBS Journal 27 3 (20 06) 24 47 24 60 ª 20 06 The Authors...

Ngày tải lên: 23/03/2014, 11:20

... (PAK2) [28 ], MAPK kinase kinase (MEKK-1) [29 ], MAPK kinase kinase kinase (Raf-1), protein kinase B (Akt) [30,31] and protein kinase C (PKC) [ 32] PKC is a family of serine ⁄ threonine protein kinases ... adhesion complexes consist of integrin proteins, the tyrosine kinases Src and focal adhesion kinase (FAK), actin-binding structural proteins, and the adaptor proteins paxillin and Crk-associated substrate ... tricarbonyl J Neurosci 22 , 1738–1751 Kaasinen SK, Golksteins G, Alhonen L, Janne J & ¨ Koistinaho J (20 02) Induction and activation of protein kinase Cd in hippocampus and cortex after kainic acid treatment...

Ngày tải lên: 23/03/2014, 11:20

... Following intron (kb) Exon PP17b ADFP PLIN PP17b ADFP PLIN ADFP PLIN PP17b ADFP PLIN 111 82 198 84 28 5 20 1 126 943 – 54 52 195 84 28 5 183 135 879 – 1 02 58 20 4 84 26 4 174 1 92 246 1577 – 22 66 28 ... 1486. 726 5 1707.9407 1834. 929 9 20 59.0460 20 66.9783 20 73. 126 6 20 76.0 726 20 89. 121 5 125 117 93 71 62 62 62 44 47 )44 71 52 49 47 61 59 pyroGlu 48–55 48–55 65–74 1– 12 1 32 1 42 63–74 65–76 114– 124 114– 124 ... 28 95 67 42 114 – – 10 65 28 95 61 45 133 – – 15 68 28 88 58 64 82 129 – 18 /27 56/ 82 54/75 43/70 36/70 26 /50 40/70 – – 21 / 42 38/68 54/79 20 / 42 13 /29 14/40 15/38 – 6 .2 1.3 0.1 7.3 4.1 2. 9 5.1 –...

Ngày tải lên: 23/03/2014, 21:20

... mechanics theory of Gent and coworkers [23 -25 1 and contributed to by Andrews and Kinloch [26 -29 1 This defines a linear relationship between the mechanical work of separation, w,, and the thermodynamic ... adhesive i (c) Fig Definition of thermodynamic work of adhesion, WA: (a) disjoining surfaces in vacuum; (b) disjoining surfaces in fluid medium m; and (c) disjoining surfaces in presence of vapors ... not directly influence the intrinsic interfacial strength, but instead contribute to the total of the stresses existing at an interface in a mechanical test In a mechanical test, interfacial strength...

Ngày tải lên: 26/04/2014, 15:36

... Male 56 22 (39.3) 34 (60.7) Female Age 48 21 (43.8) 27 (56 .2) ≥55 y 54 22 (40.7) 32 (59.3)

Ngày tải lên: 18/06/2014, 19:20

... Pesticides-Borates” Washington DC 20 01 Murray FJ: A Human Health Risk Assessment of Boron (Boric Acid and Borax) in Drinking Water Regul Toxicol Pharmacol 1995, 22 :22 1 23 0 Brockman RP, Audette RJ and Gray M: ... Phytohemagglutinin was added in RPMI medium as the mitogen and incubated for 72 hours After 72 hours incubation, a total of × 105 cells/well from each lymphocyte culture were selected in the 24 -well ... cells in tissue culture provides a potential mechanism for increasing invasive mammary carcinoma [5] and induced colonic neoplasia in animal models [6] Borax is used as a food additive in some...

Ngày tải lên: 20/06/2014, 00:20