structure of writing a film review

... conformation. Database Structural data are available in the protein Data Bank under the accession number 3OQS. Structured digital abstract l CLIC4 and importin alpha bind by x-ray crystallography ... reflection data file was then passed through a suite of programs in the ccp4 crystallography package [36]. Scaling of intensities and inspection of data quality were performed in scala. In particular, ... dimensions of approximately a =7 9A ˚ , b =9 0A ˚ and c = 100 A ˚ (see Table 1). The final diffraction dataset was obtained over 180 images with a 1° oscillation angle (u) and 5-s exposure time per image....

Ngày tải lên: 14/02/2014, 19:20

... nonoxidative decarboxylation catalysed by a- amino-b-carboxymuconate-e-semialdehyde decarbox- ylase. J Am Chem Soc 129, 9278–9279. 20 Fukuoka SI, Ishiguro K, Yanagihara K, Tanabe A, Egashira Y, Sanada ... Walker AL, Iwaki H, Hasegawa Y & Liu A (2005) Kinetic and spectroscopic characterization of ACMSD from Pseudomonas fluorescens reveals a penta- coordinate mononuclear metallocofactor. J Am ... model of cerebral malaria, a frequently fatal com- plication of Plasmodium falciparum infection; in the same model, pharmacological reduction of PA levels was demonstrated to correlate with a better...

Ngày tải lên: 18/02/2014, 06:20

... alpha- sarcin: a study of relationships. Meth Enzymol 341, 335–351. 3 Lacadena J, A ´ lvarez-Garcı ´ a E, Carreras-Sangra N, Herrero-Gala ´ n E, Alegre-Cebollada J, Garcı ´ a- Ortega L, On ˜ aderra ... Nucleic Acids Res 4, 1097–1110. 2 Martı ´ nez-Ruiz A, Garcı ´ a- Ortega L, Kao R, Lacadena J, On ˜ aderra M, Manchen ˜ o JM, Davies J, Martı ´ nez del Pozo A & Gavilanes JG (2001) RNase U2 and alpha- sarcin: ... were analyzed in a semi-automated iterative manner by cyana 2.1 [38]. The NOE coordinates and intensities used as input for automated analysis were generated auto- matically by Sparky based on...

Ngày tải lên: 18/02/2014, 08:20

... The fact that EDTA effects a marked stabilization (Table 1), however, indi- cates an additional destabilizing effect by calcium ions. Destabilization of PLDa2 by calcium ions was also indicated ... in the absence and presence of NaCl and CaCl 2 at pH 5.5 and 23 °C are compared. The results show that the instability of PLDa2is B C A D Fig. 4. Spectra of native PLDa2. (A) UV absorbance spectrum. ... modified Scatchard plot of these data, with DA being the change in relative activity (Fig. 7C), shows two linear parts, indicating that at least two separate calcium-binding events affect PLDa2 activity. A B Fig....

Ngày tải lên: 19/02/2014, 02:20

... that protein family. In addition, we show that both its fold and potential surface partially resemble the structural features of the antimammalian scorpion a- neurotoxins and of the human antibacterial ... the inactivation of the Na + channel in a fashion similar to that of scorpion a- toxins [11], other experiments have shown that, at low doses, it has an analgesic activity involving both central and ... triple-stranded antiparallel b-sheet was Fig. 3. Summary of data used in the determination of the secondary structure of crotamine. (A) Primary sequence of Crt with the sequential and medium-range...

Ngày tải lên: 20/02/2014, 11:20

... a ccordance with the Pacific Institute of B ioorganic Chemistry Policy o n Human Care and Use of Laboratory Animals. Results Isolation and characterization of lipid A from M. vaga Extraction of ... for Bioorganic Research, Osaka, Japan The c hemical s tructure of a novel lipid A, the major com- ponent of the lipopolysaccharide from the marine gamma- proteobacterium Marinomonas vaga ATCC 27119 T ,was determined ... endotoxin antagonism have a disaccharide or monosaccharide backbone, mainly one phosphate group, and low degree of acylation [8–10]. Lipid A acylation patterns, which are important in binding bacteria...

Ngày tải lên: 07/03/2014, 15:20

... 5Â-aataatccatggggagtaatacttcctggcgta aaagtgaagtcc-3Â and 5Â-attattggatccctattgctggtccgattctgccag-3Â. 3-TPR NlpI primers (residues 62197) were 5Â-aataatccatgg gggcacagcttttatatgagcgcggag-3Â and 5Â-aataatggatcctcactgttc cttatccgatttttcgaagtgc-3Â. ... the sharp curvature of the array and reduced superhelical pitch, leading to the formation of a globular structure. Helix a Residues Deviation b,c A AB pair W c AB W c AAÂ Rotation d (AB) (A BÂ) Helix ... chemically syn- thesized by the W. M. Keck Core Facility (Yale University, New Haven, CT, USA). Primers to amplify mature NlpI (residues 20294) were 5Â-aataatccatggggagtaatacttcctggcgta aaagtgaagtcc-3Â...

Ngày tải lên: 07/03/2014, 16:20

... per frame. Native data were collected to a resolution of 1.4 A ˚ . A total of 360 frames of data with an oscillation angle of 0.45° were collected. The exposure time was 0.5 s per frame. All datasets ... Frederick CA, Wang AH & Rich A (1987) A bifurcated hydrogen-bonded conformation in the d (A. T) base pairs of the DNA dodecamer d(CGCAAATTTGCG) and its complex with distamy- cin. Proc Natl Acad Sci ... & Tucker PA (2005) Auto-Rickshaw: an automated crystal structure determination platform as an efficient tool for the validation of an X-ray diffraction experiment. Acta Crystallogr D 61, 449–457. 61...

Ngày tải lên: 15/03/2014, 23:20

... purification, characteriza- tion and structural determination of charybdin, a novel 29-kDa protein from bulbs of C. maritima agg. Charybdin was characterized by biochemical methods and its structure ... Access to Research Infrastructure Action FP6 program. References 1 Gemmill CL (1974) The pharmacology of squill. New York Acad Med Bull 50, 747–750. 2 Narayanan S, Surolia A & Karande AA (2004) ... characterization and cry- stallization of charybdin. The yield of the purified pro- tein was 150–200 mg protein per 100 g of bulbs. Charybdin appeared as a single band with a molecular mass of...

Ngày tải lên: 16/03/2014, 14:20

... on Innovative ap- plications of artificial intelligence, AAAI’97/IAAI’97, pages 598–603. AAAI Press. Michael Collins and Terry Koo. 2005. Discrimina- tive reranking for natural language parsing. ... Processing of the AFNLP, pages 522–530, Suntec, Singapore, Au- gust. Association for Computational Linguistics. Canasai Kruengkrai, Kiyotaka Uchimoto, Jun’ichi Kazama, Yiou Wang, Kentaro Torisawa, and ... Proceed- ings of ACL-08: HLT, pages 897–904, Columbus, Ohio, June. Association for Computational Linguis- tics. Wenbin Jiang, Liang Huang, and Qun Liu. 2009. Au- tomatic adaptation of annotation standards:...

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