structure of an mrm application

... from the analogous structures of the ascomycete laccase (asco-laccase) MaL, thus giving a comprehensive view of the structure and function of asco-laccases Results and Discussion Overall structure ... Streptomyces antibioticus [5] Laccases are common in fungi, and are also found in some higher plants and bacteria These enzymes are capable of oxidizing various organic and even inorganic substrates; ... skilled assistance, and B Hillebrant-Chellaoui at VTT for skilful help with the TaLcc1 purification Roal Oy and the Academy of Finland (Project 115085) supported the work at Joensuu, and part of the...

Ngày tải lên: 14/02/2014, 18:20

... of MBP and MBP fragments [38–40] and support the inclusion of loose dihedral angle restraints in the structure calculations of FF2 in TFE-d2 and DPC-d38 (see below) NOE analysis The pattern and ... the stretch of residues between P5¢ and P16¢ Fig Amino acid sequence of the FF2 polypeptide, and survey of sequential and medium-range NOEs, and conformation-dependent chemical shifts of FF2 dissolved ... spectra of FF2 in TFE and DPC show the characteristic double minima at 207 nm and 222 nm of an a helix In contrast, the spectra of FF2 in 100 mM KCl and pure H2O are indicative of a primarily random...

Ngày tải lên: 19/02/2014, 07:20

... to the methyl resonance of 4,4-dimethyl-4-silapentane-1-sulfonic acid used as an internal standard Distance restraints and structure calculations An initial survey of distance constraints was ... Fig Comparison of 3D structure of mr3e (M-1 branch conotoxin) and mr3a (M-2 branch conotoxin) (A,B) Backbone conformation of mr3e and mr3a (C,D) Surface representation of mr3e and mr3a Blue regions ... rmsd of 0.56 ± 0.16 A and a ˚ mean global heavy atom rmsd of 1.30 ± 0.28 A Finally, refinement of the structure was carried out using amber [12] for energy minimization An ensemble of 20 structures...

Ngày tải lên: 30/03/2014, 09:20

... (Permanence of Paper) library of c ongre ss c ataloging -in-public ation data Headrick, Annabeth The Teotihuacan trinity : the sociopolitical structure of an ancient Mesoamerican city / Annabeth ... Teotihuacán—Politics and government Aztecs—Mexico—San Juan Teotihuacán—Rites and ceremonies Aztecs— Mexico—San Juan Teotihuacán—Antiquities Excavations (Archaeology)—Mexico—San Juan Teotihuacán San Juan Teotihuacán ... Teotihuacan and the appearance of the Teotihuacan state outside the Valley of Mexico This suggests that the manufacture and trade of obsidian were at least partially responsible for an increased...

Ngày tải lên: 11/06/2014, 15:57

... mean error ((c), (d)) of stem profile models as a function of relative height class Profile and structure of Atlas cedar tree stem 71 Figure Mean bias ((a), (b)) and mean error ((c), (d)) of ... Figure Application of the stem profile model (models IIa and IIb) and the ring profile model to the ring profiles of the trees in the Luberon region Mean bias (a) and mean error (b) as a fonction of ... analysis of fire resistance and survival of mediterranean and western north american conifers, in: Proceedings of the 12th Conference on Fire and Forest Meteorology, October 26–28, 1993, Jekyll Island,...

Ngày tải lên: 08/08/2014, 14:20

... N.I.H grant AI40882 We would like to thank Duncan McRee, Isaac Hoffman, Robin Rosenfeld and the staff at Active Sight, San Diego, for assistance in crystallization screening We thank the staff of ... formation of two intra-flap interactions: a packing contact formed between the Cε atom of Met5546 and the side chain of Phe6253, and an electrostatic interaction between the Sδ atom of Met5546 and ... better understanding of the molecular basis of HIV-1 and FIV protease (PR) substrate specificities in vivo, and are relevant to the development of broad-spectrum protease inhibitors that can inhibit...

Ngày tải lên: 13/08/2014, 09:20

... NOI UNIVERSITY OF LANGUAGES AND INTERNATIONAL STUDIES FACULTY OF POST-GRADUATE STUDIES NGUYỄN THỊ MẾN THE MEANING AND STRUCTURE OF AN AMERICAN SHORT STORY: A SYSTEMIC FUNCTIONAL ANALYSIS (CẤU ... 2: THE MEANING AND STRUCTURE OF THE TEXT “WITCHES’S LOAVES” 12 2.1 The Chosen Text 12 2.2 Clauses and Clause Complex Analysis 132 2.3 The Analysis of the Text ... Terms of Transitivity, Mood and Theme…… 12- 28 2.3.1 The Transitivity Pattern of the Text……………… 28-29 2.3.2 The Mood Pattern of the Text……………… 29 2.3.3 The Thematic Pattern of the...

Ngày tải lên: 28/02/2015, 11:55

... Cassell 16 Van, Hoang Van (2005c) The meaning and Structure of a Science Fiction Story: a systemic functional analysis Journal of Science (Foreign Languages) No.2 Pp 28-45 17 Van, Hoang Van (2006) ... functions in the structure of the CLAUSE AS AN EXCHANGE A clause has meaning as an exchange, a transaction between speaker and listener; the Subject is the warranty of the exchange It is the element ... The meaning and structure of a fairy tale story: a systemic functional analysis by Pham Thi Thuy and a research on The meaning and Structure of a Science Fiction Story: a systemic functional analysis...

Ngày tải lên: 28/02/2015, 11:55

... (syntax), semantic and discoursal problems of a text” (Hoang Van Van 2006: 161) This theory encompasses all levels of language: phonology, lexicogrammar, semantics and context of situation where language ... that text is a unit of meaning and is functional; it is functional in the sense that it occurs in a context of situation Hoang Van Van in his work “The meaning and structure of a science fiction ... it as a locution or an idea Within the general categories of expansion and projection, we recognized first of all a small number of subtypes: three of expansion, and two of projection The names...

Ngày tải lên: 02/03/2015, 14:22

... introducing important concepts in systemic functional linguistics relevant to the topic of the study and knowledge surrounding the kind of fairy tales - Chapter 3: The meaning and structure of an English ... descriptive and analytic Descriptive method is concerned with the description of main areas of functional grammar and analytic method is concerned with the analysis of the text 1.5 Design of the ... aspects of meaning and structure of the fairy tale but only some precious features are discussed They are the transitivity, the mood, the theme pattern and the cohesion of the text 1.4 Methods of...

Ngày tải lên: 10/08/2015, 19:48

... The meaning and structure of a fairy tale story: a systemic functional analysis by Pham Thi Thuy and a research on The meaning and Structure of a Science Fiction Story: a systemic functional analysis ... format and methods employed in the previous M.A Theses in the same file supervised by Prof Dr Hoang Van Van such as: The meaning and Structure of a Biology text: a systemic functional analysis ... understand the story further in terms of both meaning and structure 1.2 Aims and Scope of the Study Within the framework of a minor thesis, the aim of the study is to:  Examine some basic analytical...

Ngày tải lên: 10/08/2015, 19:52

... development of urban wards 6-158 6-160 6.1.1 Shi 市 (Market) and Fang 坊 (Ward) as the basic urban unit 160 6.1.2 Fangxiang (坊巷) as an integrated urban unit 161 6.2 Growth and transformation of Lin an and ... truths, multiple types and rules of evidence, and multiple twists of the kaleidoscope of human behaviour, many of which have legitimacy and meaning, but none of which is ‘the answer’” Thus, it could ... components of identity, structure and meaning, and narrowed down the discussions only on the physical and perceptible aspects (identity and structure) , with an ignorance of the functional and symbolic...

Ngày tải lên: 17/09/2015, 17:20

... chains of Pro34 and Leu38 N1 and N6 of the base of A20 lie close to Nd2 and Od1 of the side chain of Asn87 in the S4 strand, with ˚ ˚ distances of 2.82 A and 2.97 A, respectively The plane of the ... between the a-NH2 group of Arg and the main chain C@O of Ser151 and O of the side chain of Asn153, and the distances between the guanidinium moiety and the side chains of Glu148 and Asp351 in S cerevisiae ... distances between the a-NH2 group of Arg and the main chain C@O of Ser127 and ˚ O of the side chain of Asn129 are set at 3.15 A and ˚ , and the distances between the guanidinium 3.04 A moiety and...

Ngày tải lên: 18/02/2014, 11:20

... specific binding modes of these 1,3-BPGA analogues to the two trypanosomatid enzymes Materials and methods Sources of substrates, cofactors and inhibitors The synthesis of 1,3-BPGA analogues used in ... effect of these substrate analogues This is consistent with a simultaneous interaction of the 1,3BPGA analogues at both Ps and Pi phosphate-binding sites Comparison of the inhibition of T cruzi and ... ´ 23 Duee, E., Olivier-Deyris, L., Fanchon, E., Corbier, C., Branlant, G & Dideberg, O (1996) Comparison of the structures of wildtype and a N313T mutant of Escherichia coli glyceraldehyde-3phosphate...

Ngày tải lên: 20/02/2014, 02:21

... different in all of the tetrameric PDCs of known three-dimensional structure Binding of the cofactors ThDP and Mg2+ The homo-tetrameric IPDC binds four molecules of the cofactors ThDP and Mg2+ The ... Sweden and the Graduiertenkolleg of Sachsen-Anhalt References Bartel, B (1997) Auxin biosynthesis Ann Rev Plant Physiol Plant Mol Biol 48, 51–66 Normanly, J & Bartel, B (1999) Redundancy as a way of ... Opin Plant Biol 2, 207–213 Kawaguchi, M & Syono, K (1996) The excessive production of indole-3-acetic acid and its significance in studies of the biosynthesis of this regulator of plant growth and...

Ngày tải lên: 20/02/2014, 11:20

... backbone w angle restraints were obtained from analysis of dNa/daN ratios [38] The w angle restraint was set to )30° ± 110° for dNa/ daN ratios less than 1, and to 120° ± 100° for dNa/daN ratios ... importance of this helix for membrane-bound receptor recognition and consequent antimicrobial activity [2,10] For example, Fimland et al [42] have shown that interchange of large domains of different ... transporter proteins are usually found in these bacteriocin operons [5] ABC transporters are a family of large transmembrane proteins responsible for the ATP-driven transport of a variety of...

Ngày tải lên: 07/03/2014, 15:20

... one another than to any other profile in the data set The profiles of G can be summarized by a vector s whose dimensionality is that of the data, and each of whose elements contains the mean of ... prophethood of Mohamed and the coming resurrection, and remind humanity of the past prophets and events of their times On the other hand, the Madinan suras outline ritualistic aspects of Islam, ... account of any nonlinearities in the data References “Figure Plot of groups C and D” Results from figure are also supportive of the thematic structure of each group Suras of group C are more abundant...

Ngày tải lên: 08/03/2014, 04:22

... sequential and 134 medium range) constraints, which were used to generate an ensemble of 100 structures by the standard protocol of simulated annealing in torsion angle space implemented in DYANA (using ... quartz cell of 1.0-mm path length Spectra were collected over the wavelength range 260–190 nm with a bandwidth of 2.0 nm and a time constant of 8.0 s, and corrected for the contribution of the buffer ... membrane [38] Accordingly, it is possible that the mechanism of membrane interaction and destabilization is different in the case of Ab, Fig Comparison of the shapes of the lowest energy structure...

Ngày tải lên: 08/03/2014, 09:20

... ÔprototypeÕ plant annexin Anx(Gh1) shares 72% identity with Anx24(Ca32) and probably belongs to the class of Sp32 annexins In the current study, we purified and crystallized recombinant Anx(Gh1) and determined ... Several mammalian annexins and even more plant annexins show conservation of these two cysteine residues and some of them have been implicated in oxidative stress X-ray structure of Anx(Gh1) (Eur ... protein which can be crystallized much more successfully than the other ones Ó FEBS 2003 2560 A Hofmann et al (Eur J Biochem 270) Structure of Anx(Gh1) in comparison with Anx24(Ca32) and AnxA5 The...

Ngày tải lên: 17/03/2014, 03:20

... obtain a better understanding of the importance of disulfide bridges and the hydrophobic-hydrophilic balance on the antimicrobial activity of gomesin, synthetic analogues of this peptide that lack ... the disruption of the membrane integrity, probably through the formation of pores [40] The similarities between the three-dimensional structure of gomesin and those of PG-1 and androctonin suggest ... the primary structure of the peptide Structure evaluation The three-dimensional structure of gomesin was determined using the standard simulated annealing protocol of DYANA AND energy minimization...

Ngày tải lên: 17/03/2014, 23:20