structure function relationships in t4 and l
Báo cáo khoa học: Structure–cytotoxicity relationships in bovine seminal ribonuclease: new insights from heat and chemical denaturation studies on variants ppt
Ngày tải lên :
22/03/2014, 17:20
... were plated on 96-well plates at a density of 2.5 · 103 cells per well in 100 lL of medium containing BS-RNase or one of its variants (12.5, 25, 50 or 100 lgÆmL)1), and incubated for 24 and 48 ... Giancola et al 35 Koradi R, Billeter M & Wuthrich K (1996) MOLMOL: a program for display and analysis of macromolecular structures J Mol Graph 14, 51–55 36 DeLano WL (2002) The PyMOL Molecular ... Sali A (2006) Comparative protein structure modeling using Modeller Curr Protoc Bioinformatics Chapter 5, Unit 34 Fiser A, Do RK & Sali A (2000) Modeling of loops in protein structures Protein...
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Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): structure–function relationships docx
Ngày tải lên :
16/02/2014, 15:20
... total oxygen in humans, and also Cu, Fe and Zn are heavily deposited in the senile plaque and neurofibril tangle Any disorder or malfunction in the metalloproteins and metalloenzymes that maintain ... b-domain), which are closely associated with the mutual accessibility of metal–thiolate clusters with biologically sensitive small molecules such as NO, thus in uencing zinc homeostasis in the brain ... tightly and alter the domain–domain interactions in hGIF through intramolecular interactions, and eventually affect the structural and dynamic properties of the b-domain by domain– domain interactions,...
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Báo cáo khoa học: New insights into structure–function relationships of oxalyl CoA decarboxylase fromEscherichia coli pptx
Ngày tải lên :
06/03/2014, 11:20
... 30 lM; triangles, 60 lM; inverse triangles, 120 lM; lines, hyperbolic fits) (B) Plots of v against [S] in the absence (open circles) and presence of 60 lM (filled triangles) and 300 lM ADP (filled ... Superposition of structure models of the ADP-EcODC complex in crystal and solution The crystal structure of 2q28 is shown in ribbon and line style in deepsalmon, the solution structure model of ADP-ThDP-EcODC ... of 300 lm ADP, an activator of ODC catalysis, resulted in a marginal increase in kcat and a small decrease in KM, leading to a 1.7-fold higher catalytic efficiency (Fig 7B and Table 2) Similar weak...
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Báo cáo khoa học: Evolutionary changes to transthyretin: structure–function relationships ppt
Ngày tải lên :
07/03/2014, 00:20
... and most proteins in the CSF (including albumin and thyroxine-binding globulin) originate from the blood and move across the blood–brain barrier [48,49] However, this is not likely to be the situation ... transthyretin: the role of the N-terminal Mol Cell Endocrinol 295, 48–58 56 Ronne H, Ocklind C, Wiman K, Rask L, Obrink B & Peterson PA (1983) Ligand-dependent regulation of intracellular protein transport: ... 467–474 Kato M, Kato K, Blaner WS, Chertow BS & Goodman DS (1985) Plasma and cellular retinol-binding proteins and transthyretin (prealbumin) are all localized in the FEBS Journal 276 (2009) 5330–5341...
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Báo cáo khoa học: Studies on structure–function relationships of indolepyruvate decarboxylase from Enterobacter cloacae, a key enzyme of the indole acetic acid pathway ppt
Ngày tải lên :
08/03/2014, 02:20
... derivatives a-keto glutarate and b-phenylpyruvate inhibit EcIPDC activity Indole and some similar metabolites such as L- tryptophan, indole-3-lactate, indole3-acetaldehyde, tryptophol, and indole-3-acetate ... of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 14 Hallmann, G & Hagle, H (1963) Benzofuranderivate: Trypta¨ min-, Serotonin- und Melatonin-Analoga Liebigs ... squares and full lines, dimers; triangles and dashed lines, tetramers; lines are drawn for better visualization only.) 2326 A Schutz et al (Eur J Biochem 270) ¨ Ó FEBS 2003 indolepyruvate (triplets...
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Báo cáo khóa học: The structure–function relationship in the clostripain family of peptidases potx
Ngày tải lên :
23/03/2014, 12:20
... hypothesis In order to explore other aspects of the structurefunction relationship of clostripain and its homologues, a molecular model would be invaluable This would obviously require a reliable alignment ... we investigate the structureactivity relationship of clostripain, and its homologues in the peptidase C11 family, by afnity labelling, site-directed mutagenesis and molecular modelling A catalytic ... competent Escherichia coli JM105 cells E coli harbouring plasmid pChC were grown at 37 C in L LuriaBertani medium containing 100 lgặmL)1 ampicillin The synthesis of clostripain was induced by the addition...
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Báo cáo khoa học: Protein tyrosine phosphatases: structure–function relationships ppt
Ngày tải lên :
30/03/2014, 04:20
... understanding the multiple interactions that can take place between ectodomains, transmembrane and intracellular regions, will require structures of full-length receptors, alone and in complex with ligands ... structural and functional evidence will be required to fully understand and validate such models Structure and role of the intracellular region With few exceptions (type VIII RPTPs that are catalytically ... opposing cells (cell and 2) are shown by gray rectangles, the full-length C-cadherin ectodomain structure is in blue (Ca2+ atoms in red, PDB accession number 1L3 W) The C-terminal fibronectin type...
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proverbs denoting family relationships in english and vietnamese a contrastive analysis and teaching implications.” = các tục ngữ chỉ mối quan hệ gia đình một phân tích đối chiếu và những ứng dụng trong dạy họ
Ngày tải lên :
02/03/2015, 14:36
... proverbs denoting family relationships in English and Vietnamese also carry both literal meaning and figurative meaning It will be more explicit in the following examples: (1) Môi hở l nh [10: 354] ... teaching and learning English and Vietnamese in general and proverbs in particular, as far as the author is concerned, is their cultural awareness and quick-minded and active ability in applying language ... proverbs denoting family relationships in English are normally more complex than those in Vietnamese in terms of sentential patterns Proverbs denoting family relationships in English exist in three...
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Structure function relationships of variegin a novel class of thrombin inhibitors
Ngày tải lên :
14/09/2015, 14:11
... Savignin Monobin Amblin Boophilin Hemalin • • • Slow, tight-binding, competitive inhibition N-terminal Kazal domain inhibits active site canonically C-terminal Kazal domain binds to exosite-I (Steiner ... vascular endothelial cells following injury) and, to a lesser degree, urokinase (synthesized as a zymogen prourokinase by epithelial cells lining excretory ducts and is activated by proteolytic cleavage) ... et al., 1999) and infestin from Triatoma infestans (Campos et al., 2002) Typically, these molecules consist of multiple non-classical Kazal domains and bind to thrombin in a slow, tight-binding,...
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Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc
Ngày tải lên :
19/02/2014, 16:20
... Campagnolo, M., Schinzel, R & Johnson, L. N (2002) Enzymatic catalysis in crystals of Escherichia coli maltodextrin phosphorylase J Mol Biol 322, 413423 Supplementary material The following material ... was obtained under conditions comparable to the ones used by Shaltiel et al [6] for resolution of RmGP; i.e using imidazole citrate and L- cysteine as structure- deforming and PLP-resolving reagents, ... reconstituted EcMalP may yield a small fraction B depending on the applied amount of protein Protein fractions AC were characterized functionally and structurally, as summarized in Table Production and characterization...
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Báo cáo khoa học: Change in structure of the N-terminal region of transthyretin produces change in affinity of transthyretin to T4 and T3 pdf
Ngày tải lên :
23/03/2014, 10:21
... of the main functions of TTR, which is functionally integrated with albumin and thyroxine-binding globulin as a network system to ensure the appropriate extracellular and intracellular distribution ... release by the KEX2 gene product, and cleavage by KEX2 occurs between arginine and glutamine in the sequence Glu-Lys-Arg-Glu-Ala-Glu-Ala According to Invitrogen, the sequence of Glu-Ala-Glu-Ala ... retinol-binding protein and thyroid hormones showed that the recombinant TTRs retained its function as binding protein for retinol-binding protein and thyroid hormones In the present study, the Kd values...
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Báo cáo y học: "Tibialis posterior in health and disease: a review of structure and function with specific reference to electromyographic studies" pot
Ngày tải lên :
10/08/2014, 21:23
... examination including techniques such as manual muscle testing and the single heel rise test [7,8] Clinical examination can be supplemented with more specialist modalities including muscle function ... retromalleolar region Gross anatomy of retromalleolar region indicating flexor digitorum longus tendon (FDL), tibialis posterior tendon (TP), medial malleolus (M) and tendo Achilles (TA) Small arrow ... discomfort and could alter normal walking There are two anatomical approaches for inserting intramuscular EMG electrodes into the TP muscle belly: (i) the posterior-medial; and (ii) the anterior insertion...
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Báo cáo khoa hoc:" Effects of bone marrow cell transplant on thyroid function in an I131-induced low T4 and elevated TSH rat model" docx
Ngày tải lên :
11/08/2014, 08:20
... cells have shown board plasticity that has allowed treatment of heart, liver and principally, blood related disorders, [1,4] This plasticity and the availability of adult bone marrow stem cells ... transplantation of stem cells TSH and T4 serum levels of all groups weeks after BM derived cells transplantation All concentrations of T4 are very similar in all groups with no statistical difference between ... Histological cuts One lobe of the thyroid gland of each rat was obtained and fixed in 4% paraformaldehyde overnight and stained using hematoxylin-eosin (H&E) Histological cuts were examined under...
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Báo cáo y học: " Inhaled salmeterol and/or fluticasone alters structure/function in a murine model of allergic airways disease" potx
Ngày tải lên :
12/08/2014, 11:20
... of fluticasone and salmeterol, both separately and in combination, on lung structure and function in allergically inflamed mice Salmeterol alone worsened airways hyperresponsiveness and increased ... think that an increase in mucus containing cells or mucus within the airway, in combination with epithelial injury or increased inflammation too subtle to be quantified by simple histological ... methacholine challenges (saline control and methacholine at 3.125, 12.5, and 50 mg/ml) Methacholine was nebulized for 40 seconds with the inspiratory line of the ventilator connected through a nebulizer...
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Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf
Ngày tải lên :
14/02/2014, 18:20
... hierarchical folding, axial glue model of chromosome structure J Cell Biol 166, 775–785 50 Muller WG, Walker D, Hager GL & McNally JG (2001) Large-scale chromatin decondensation and recondensation regulated ... other’s binding to DNA within nucleosomes This principle is illustrated in the model shown in Fig 7A In this scenario, factor B is only able to bind to an internal bind- A Nucleosome length DNA ... Nucleosomes tend to naturally assemble at intrinsically defined positions, determined by the underlying sequence, and nucleosome positioning evolves in parallel with the specific mode of regulation...
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Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx
Ngày tải lên :
14/02/2014, 18:20
... residues directly involved in ligand binding, presumably abolishing the interaction with signaling partners The remaining mutations alter amino acids located outside the ligand-binding pocket and reduce ... Patients with XLA have a differentiation block resulting in an almost complete absence of B lymphocytes and plasma cells and very low levels of immunoglobulins of all classes Humoral immune responses ... studied extensively in mouse models ITK regulates a number of T-cell signaling pathways, including NKT cell development and functions; in ITK-deficient mice, the overall NKT percentage and numbers...
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Tài liệu Personal Relationships of Dependence and Interdependence in Law pdf
Ngày tải lên :
18/02/2014, 05:20
... (1972) 79 Yale L. J 1049 Personal Relationships of Dependence and Interdependence in Law Dependence in Client-Therapist Relationships: A Relational Reading of O’Connor and Mills Sue Campbell In this ... setting certain limits to some contractual obligations: rendering certain abusive clauses illegal; making disclosure of certain information compulsory; and allowing consumers to change their minds ... capacities, and values are actually formed.11 We develop and live our lives as persons within complex networks of institutional, personal, professional, interpersonal, and political Dependence in Client-Therapist...
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Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc
Ngày tải lên :
18/02/2014, 16:20
... both a novel C-terminal actin-binding submodule (CABS) containing a novel actin monomer binding verprolin homology C-terminal (VH2-C) domain and a second submodule comprising the previously characterized ... actin nucleation and myosin motor activity Dev Cell 11, 33–46 Donnelly SF, Pocklington MJ, Pallotta D & Orr E (1993) A proline-rich protein, verprolin, involved in cytoskeletal organization and ... formaldehyde, permeabilized, and F-actin stained with Alexa-488-conjugated phalloidin Stained cells were viewed using fluorescence microscopy Fields containing small-budded cells were specifically chosen to...
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Tài liệu Báo cáo khoa học: Phylogenetic relationships in class I of the superfamily of bacterial, fungal, and plant peroxidases pptx
Ngày tải lên :
19/02/2014, 16:20
... listed in Table 1) located in the distal heme cavity is invariantly conserved among all N-domains of catalase– peroxidases, all CCPs and all APXs Whereas the essential arginine (Arg92) and histidine ... thaliana Arabidopsis thaliana Archaeoglobus fulgidus Aspergillus fumigatus Emericella nidulans Bacillus halodurans Geobacillus stearothermophilus Blumeria graminis Burkholderia cepacia Burkholderia ... residues and motifs present in all known KatGs All sequenced N-terminal domains are longer (average length 430 amino acids) because of several insertions not present in C-terminal domains (309 amino...
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Tài liệu Báo cáo Y học: Structure of the O-polysaccharide and classification of Proteus mirabilis strain G1 in Proteus serogroup O3 potx
Ngày tải lên :
21/02/2014, 15:20
... incubated with 25 lL twofold serially diluted inhibitor in microtitrate plates After incubation (15 min, 37 °C), 50 lL sensitized sheep red blood cells and 25 lL complement were added, the plate ... Anti-(O-polysaccharide) serum was serially twofold diluted with 50 lL veronal buffered saline and, after adding 50 lL antigen suspension and 25 lL guinea-pig complement diluted (1 : 20) with veronal ... against P mirabilis G1 and S1959 with alkali-treated P mirabilis LPS in EIA Sheep red blood cells were used as control Reciprocal titre for alkali-treated LPS from Origin of alkali-treated LPS...
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