structure function and applications

... Analytical enzymes and enzyme substrates were specied in previous papers [1518] All other chemicals were of reagent grade and obtained from Sigma and Fluka Preparation of apo-Cc StP and apo-Ec MalP ... citrate and 0.1 M cysteine hydrochloride, in short, the resolution buffer Various pH values between 5.0 and 8.0 were tested, and a pH of 7.0 was chosen (see below) Prior to the resolution, CcStP and ... R226A and R242A mutants (Table 1), all enzymes were stable for h in the presence of mM potassium phosphate and potassium sulphate Reconstitutions with PLP of apo-Cc StP and mutants thereof, and...

Ngày tải lên: 19/02/2014, 16:20

Ngày tải lên: 23/03/2014, 03:20

... the mouse, fly and worm, and more exciting discoveries are no doubt on the way On the other hand, the function of plant serpins represents an obvious deficiency in our global understanding of the ... crystallography and this meeting proved no exception; in addition to published work, 10 unpublished serpin structures were presented affording major new insights into serpin function and providing ... serpin function and dysfunction in the coagulation cascade In a related talk, Daniel Johnson (Cambridge Institute for Medical Research, UK) provided an elegant structural explanation for dysfunction...

Ngày tải lên: 30/03/2014, 20:20

... Yang Lili and Wu Yayun, for their friendship and the happy days we have been together Finally, I would like to thank my parents, my sister and brother for their love, understanding, and patience ... intriguing semantic relations and syntactic features have prompted many scholars debating its particularity and intrinsic properties This study explores the structure, function, and historical developments ... descriptive and illustrative context where Chinese extended middle construction emerges and put forwards that this construction is a double-topic nested structure The issue of how its form and meaning/function...

Ngày tải lên: 09/09/2015, 17:57

... hepatobiliary system and improve our comparative understanding of vertebrate liver structure and function These findings also raised interesting questions regarding the "functional unit" of the ... validation and application of exogenous fluorescent probes, and endogenous fluores- cence, for the in vivo study of biological structure, function, and xenobiotic response, in STII medaka, in both 2D and ... of biological structure and function at virtually all levels of organization, from subcellular to gross anatomical [12,47] Exhibiting no expression of leucophores and melanophores, and minimal...

Ngày tải lên: 13/08/2014, 13:20

... regions based on prior structural and functional imaging data that related structure to cognitive functions of interest (see Greenwood, 2007; Raz, 2005; Reuter-Lorenz and Lustig, 2005 for recent reviews) ... were standardized (z-transformed) and combined into six theoretically motivated composite scores (attention, verbal memory, visuospatial memory, speed of processing, executive functions and language) ... test (Delis et al., 2001), and the Trail Making Test B (Reitan and Wolfson, 1985) Speed of processing was assessed with the TrailMaking Test A (Reitan and Wolfson, 1985) and the Symbol-Digit Modalities...

Ngày tải lên: 14/02/2014, 06:20

... al [15] determined the structures of E75Q and E75L mutants of rcSHMT and studied their properties However, the structures determined by them were not at high resolution and suffered from a lack ... we determined the structure of E53QbsSHMT and its binary complexes with Gly, l-Ser, l-allo-Thr and ternary complex with Gly and 5-formyl-THF (FTHF) In addition, the biochemical and spectral properties ... bsSHMT (Blue) Y51 and the Schiff base are in the same orientation in E53QbsSHMT and bsSHMT, whereas the orientations of E53, Y60 and Y61 have changed ˚ wild-type and mutant structures is 0.10...

Ngày tải lên: 18/02/2014, 16:20

... hypoxanthine) and the diphospho-bridge, as they can be replaced by much simpler ether strands These ether strands mimic the distance between the nucleobase and the diphospho-bridge, but on the other hand ... permeabilized T-cells and induced both local and global Ca2+ signalling in intact T-cells [46] 8-Azido- and 8-NH2-cIDPRE performed similarly (Fig 4) whereas the halogenated compounds 8-Br- and 8-Cl-cIDPRE ... (2005) 4590–4597 ª 2005 FEBS 4591 Second messenger function of cADPR (ADPRC) and thereby induce the formation of cADPR (Fig 2) G-protein coupling and Tyr phosphorylation have been implicated in...

Ngày tải lên: 20/02/2014, 01:20

... three distinct forms, and the structure of form A solved The enzyme is a homodimer, and the structure shows two domains in the a subunit Ó FEBS 2002 Structure, mechanism and function of prenyltransferases ... molecular dyad axis The seven a helices and six b strands are shown in red and green, respectively, for subunit A, and in magenta and cyan for subunit B, and they are labelled separately The blue ... two a helices (a2 and a3) and four b strands (bB, bA, bD, and bC) as shown in Fig 5A In contrast with the symmetric structure of M luteus UPPS, two protein conformations (open and closed forms)...

Ngày tải lên: 08/03/2014, 22:20

... pedal and hypobranchial glands in different species of this class In mollusks which lay their eggs in rigid capsules, capsule glands and albumin glands are present in a complex of pallial glands ... development and functioning of the reproductive systems Octopus and cuttlefish females are functionally mature at the end of stage IV (stages V and VI occur at spawning) Female squid are usually functionally ... that during the juvenile phase and through physiological maturation, the gonad and accessory glands function asynchronously At physiological maturity these two organs function synchronously Preliminary...

Ngày tải lên: 14/03/2014, 16:20

... aminoglycosides Thus, a new set of poly arginine 6-mer and 9-mer d- and l-aminoglycoside conjugates (APACs) was designed and synthesized, and their cell uptake and antiviral activities were determined We ... HIV-1 Tat responsive element (TAR) RNA binding, NMR structures of TAR–ligand complexes and aminoglycoside–RNA interactions, we have designed and synthesized a set of conjugates of aminoglycoside ... interaction with CXCR4 and compete with its natural ligand SDF-1a to CXCR4 Results Synthesis and chemical characterization of APACs The synthesis of the regioselectively functionalized aminoglycosides...

Ngày tải lên: 16/03/2014, 06:20

... the structure and function of the enzyme by mutating Val106 to amino acids differing in polarity, size and conformation, and subsequently investigating their effect on the quaternary structure and ... [28] The amino acid sequence identity is 34% between dNK and dGK [29], and 47% between dGK and dCK [28], and the structures of dNK, dGK and dCK appeared to be very similar [27,28] Despite the ... glycerol and mM dithiothreitol) with and without 2.5 mM ATP, and incubated on ice for h before storage at )80 °C The enzymes incubated and stored with and without ATP are referred to as the +ATP and...

Ngày tải lên: 16/03/2014, 16:20

... demands are imposed on the sensitivity and selectivity of the methodology, which should provide molecular structure and dynamics information In this article, we present the potential of Raman and ... physiological processes of cytochrome c Methodology Raman and IR spectroscopy are molecular structuresensitive techniques, as the frequencies and band intensities of vibrational transitions represent ... field effects on protein structure, dynamics, and function Information provided by surfaceenhanced vibrational spectroscopy Redox proteins immobilized on SAM-coated Ag and Au electrodes are usually...

Ngày tải lên: 22/03/2014, 16:20

... types: those between aHi and NHi+6 (i ¼ 1, 3, and for chain A, and 1, 3, 5, 7, and for chain B); and those between aHi and NHi-6 (i ¼ 10 and for chain A, and 14, 12, 10, and for chain B) These ... The left-handed doublehelix structure transformed into two single-stranded right-handed b-helices with 6.3 residues per turn Whereas the left-handed double b-helix is consistent with the structure ... A and Trp15 of chain B are highly uncertain (with three and four unambiguous data points and R of 0.98 and 0.98, respectively) Fig NOESY spectrum (150 ms) in the region of (F2) 9.7–8.0 p.p.m and...

Ngày tải lên: 30/03/2014, 15:20

... performed to understand the physiological function of the 4-kDa peptide, and suggests that this peptide is involved in the regulation of callus growth and cell proliferation Tertiary structure of the ... °C, and washed twice with Tris/NaCl/Pi for Finally, the cross-reacted bands were detected with alkaline phosphatase substrate (Moss, Maryland) Results and discussion Possible physiological function ... octadecanoid Ó FEBS 2003 Possible function and structure of a 4-kDa legume peptide (Eur J Biochem 270) 1275 Fig Ribbon representation of insulin and the 4-kDa peptide (A) Solution structure of human insulin...

Ngày tải lên: 31/03/2014, 07:20

... Bullen, Handbook of Means and Their Inequalities, vol 560 of Mathematics and Its Applications, Kluwer Academic Publishers, Dordrecht, The Netherlands, 2003 10 Journal of Inequalities and Applications ... 1998 J S Aujla and F C Silva, “Weak majorization inequalities and convex functions,” Linear Algebra and Its Applications, vol 369, pp 217–233, 2003 10 F Qi, J S´ ndor, S S Dragomir, and A Sofo, ... Uchiyama, and S.-E Takahasi, “A refinement of various mean inequalities,” Journal of Inequalities and Applications, vol 2, no 4, pp 387–395, 1998 K Guan, “The Hamy symmetric function and its generalization,”...

Ngày tải lên: 22/06/2014, 02:20

... 5-pyridylboronic acids (4 and 5), were reported [13] Whereas the boronic acid group has a trigonal geometry and is fairly coplanar with the benzene ring in structures 1and 2, and and 5, it is almost ... synthetic and biological applications is presented, with an emphasis on topics not covered in other chapters 1.2 Structure and Properties of Boronic Acid Derivatives 1.2.1 General Types and Nomenclature ... diazaborolidines and diazaborinanes , and the mixed nitrogen–oxygen heterocycles are denoted by the prefix oxaza Unsaturated heterocycles are named as boroles 1.2.2 Boronic Acids 1.2.2.1 Structure and Bonding...

Ngày tải lên: 02/07/2014, 12:53

... fuel economy and pollutant capture in urban environment [50, 85] The framework of tree structure function models is primarily aimed at understanding tree growth and development Functional-structural ... interactions between tree structure, function and the environment Such models are structural-functional tree models sensu stricto and should include both instantaneous and delayed tree responses ... = H + E (5) where Rn and M are the net gains of energy from radiation and metabolism, respectively, and H and E are losses of sensible and latent heat, i.e by convection and evaporation, respectively...

Ngày tải lên: 08/08/2014, 14:22

... strains of ARV (ARV138 and ARV176) in order to expand the available ARV sequence database, determine sequences of the ARV RdRp protein, and to delineate conserved structure/ function features of ... CSRV, and AqRV GCRV are aligned (Fig 1) Thus, to gain structure/ function information about this key viral-encoded enzyme, ARV, MRV, and AqRV identical amino acids, conservative substitutions, and ... MRV, and AqRV RdRp protein sequences (Fig 1, closed circles) Amino acid identities between ARV λB and homologous MRV λ3 or AqRV VP2 are ~55% and ~42%, respectively (Table 3), suggesting the ARV and...

Ngày tải lên: 12/08/2014, 04:21

... fluticasone and salmeterol, both separately and in combination, on lung structure and function in allergically inflamed mice Salmeterol alone worsened airways hyperresponsiveness and increased ... epithelial cells containing airway mucin was greatest in the V and S groups and was significantly less in the F and FS groups (Figures and 7) There was a trend for increased PAFS positive cells in ... Groups include naïve mice (N) and OVA sensitized and challenged mice treated with vehicle control (V), salmeterol (S), fluticasone (F), and a combination of fluticasone and salmeterol (FS) Results...

Ngày tải lên: 12/08/2014, 11:20