structure and regulation of the tnap gene

... repeat and relative expression of the HD gene We then determined the structure of the mouse CB1 gene and determined that transcription of the CB1 gene was affected in striatal neurons of transgenic ... determined the sequence and structure of the 5¢-end of cDNAs corresponding to full-length mature CB1 mRNA and deduced the structure of the CB1 gene by comparing the cDNA sequences to the genomic ... sites within the CB1 gene upstream of the intron in the 5¢-UTR It is unlikely that premature termination of the reverse transcriptase reaction could generate the 5¢-end of these cDNAs as the adapter...

Ngày tải lên: 16/03/2014, 18:20

... [86] Nevertheless, the observed homodimerization of the GCD and the metal ion dependence of the catalytic activity support the integrity of the expressed proteins As expected from the high sequence ... site in the AC C1 domain [81] The N-terminal and C-terminal ends of each monomer are labeled The two-fold symmetry axis in the dimer runs perpendicular to the plane of the page The dimer structure ... lmolÆmin)1Æmg)1 to 23 lmolÆmin)1Æmg)1 [29,88–90] Together, these data seem to suggest that the structure of the Cyg12 GCD dimer may reflect the structure of the GCD in the dimerized full-length NPRA in its basal...

Ngày tải lên: 28/03/2014, 23:20

... exons On the other hand, the PM3 genes had 4-5 exons and a SUN domain that was encoded within the largest exon Comparative analysis of the maize ZmSUN gene structures revealed that the CCSD genes ... Table 1) together with the B73 reference genome, we were able to define the structures of five maize SUN-domain genes as shown in Table and Figure Three of these genes (ZmSUN1, 2, and 3) are distributed ... and exon structures within the maize SUN genes showed that the gene structures are conserved within each class The CCSD genes had two or three exons, and the SUN domain was split between the exons...

Ngày tải lên: 11/08/2014, 11:21

Ngày tải lên: 12/12/2013, 00:15

... pGEX-4T-2 The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were generated ... structural and functional importance of the v-KIND and MAP2 interaction core modules in the regulation of dendrite growth and branching in hippocampal neurons and cerebellar granule cells Further studies ... proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the KIND family of proteins, only KIND2 binds to MAP2 Pull-down assay (PD) of the endogenous MAP2 from cerebellar lysates of P21...

Ngày tải lên: 14/02/2014, 19:20

... SDS/PAGE and in 2D PAGE (Figs and 2; Table 1) The N-terminal amino acid sequences (see above) allow the assignment of the HcA clone to the major sequence of the upper hemocyanin band in the SDS/PAGE ... supports the notion of the usefulness of hemocyanin sequences for the resolution of the arthropod phylogenetic tree [4,6] Myriapod hemocyanin subunit evolution and diversity One of the most striking ... data analyses and phylogenetic studies The tools provided with Genetics Computer Group (GCG) Software Package 10 and by the ExPASy Molecular Biology Server of the Swiss Institute of Bioinformatics...

Ngày tải lên: 20/02/2014, 11:20

... human and rat CB1 receptor The CB2 antibody was raised against a sequence between the N-terminal and the first transmembrane domain of the protein of the human and rat CB2 receptor The specificity of ... Furthermore, the PCR primers for glyceraldehyde3-phosphate dehydrogenase and FAAH were selected on the basis of the sequence of the FAAH gene (NCBI accession number AF098010) by including the ... for AEA, PalEtn and 2AG, respectively Analysis of cannabinoid receptors and fatty acid amide hydrolase To determine the presence of the cannabinoid receptors (CB1 and CB2) and of the fatty acid...

Ngày tải lên: 22/02/2014, 07:20

... for the determination of the structures of the conformers of the C-domain The dipolar couplings provided long-distance information on the global folding of both conformers The structure of the ... on one side of the b-sheet and two on the other side The rmsd of the heavy atoms (Ca, C, and N) of the protein core, when the NMR structures of both conformers are superimposed on the crystal ... between the signal of the HN of Gly337 in the open conformer and the signal of the water protons All of these observations indicate that the loop region of the open conformer has a higher degree of...

Ngày tải lên: 06/03/2014, 11:20

... Additionally, because the demand of the chloroplast is ‘sensed’ inside the organelle, the IEM is closest to the origin of the signal, and thus regulation at the Tic complex could be one of the fastest ... which anchor the protein in the membrane The position and function of the long C-terminal tail on the other hand remains a matter of controversy [10,11,13–15] According to one hypothesis, the hydrophilic ... description of recent advances in the understanding of the channel-, motor- and regulatory components of the Tic complex For reference, some of the available knowledge, including the proposed function of...

Ngày tải lên: 07/03/2014, 03:20

... comparison of part of the protein backbone structure of the representative solution structure of the human eRF1 M domain and the Ca trace in the crystal structure of RF1 in the whole ribosome structure ... on the equivalent segments of each member of the family of the solution structure of the M domain of human eRF1 The resulting rmsd values (y-axis) and their deviations through the 25 NMR structures ... Fig The solution structures of the M domain of human eRF1 (A) The stereo view of the ensemble of the final 25 calculated structures superimposed on heavy backbone atoms (Ca, N and C) The poorly structured...

Ngày tải lên: 07/03/2014, 05:20

... involved in binding of the cofactor ThDP are located at the C-terminal ends of the b-strands of Dom-c (diphosphates and Mg2+) and of Dom-a¢ of a neighbouring subunit (pyrimidine moiety) The active centre ... with the gene of the benzaldehyde lyase and for helpful discussions, the teams of the Swiss Light Source (Villigen ⁄ CH) and of the EMBL outstation Hamburg for their help with data collection and ... comparison of the relative B-factors along the ThDP molecules shows that the Fig Structure- based sequence alignment of BAL, BFD and PDC all of which are used in organic synthesis The secondary structure...

Ngày tải lên: 07/03/2014, 12:20

... CHAPTER – THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT 24 THE WHEAT BOOK THE WHEAT BOOK CHAPTER – THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT THE STRUCTURE AND DEVELOPMENT OF THE CEREAL ... parts of a leaf are the tip of the blade and the top of the sheath Where the blade and sheath join, there are structures called the ligule and the auricles (Figure 2.3) Characteristics of the shoot ... structure of the stem and leaf of the wheat plant 27 CHAPTER – THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT DESCRIPTION OF THE WHEAT PLANT THE WHEAT BOOK (continued) at their bases, so the...

Ngày tải lên: 08/03/2014, 23:20

... majority of the studies have been done on the GnRH-I promoter, and there are only a few regarding regulation of the GnRH-II gene Regulation of GnRH gene expression The GnRH-I promoter In view of the ... understanding the control of its synthesis and release is therefore of the utmost importance However, it is difficult to study the regulation of GnRH gene transcription in vivo, due to the scarcity and ... differential regulation of the GnRH-I and GnRH-II genes; for instance, there are different effects of the steroid hormones E2 and P4 on the two GnRH genes Therefore, it is possible that the two forms of...

Ngày tải lên: 16/03/2014, 04:20

... proved that iron control of the Regulation of the desferrioxamine gene cluster expression of the des cluster is mediated by the DmdR1 regulator Results Deletion of the desA gene of S coelicolor blocks ... boxes) in the upstream region of the diphtheria toxin (tox) gene of C diphtheriae and the desA gene of S pilosus Several putative iron boxes were found by bioinformatics analysis upstream of 10 different ... control of the desferrioxamine cluster In this study, we report the deletion of the first gene of the desABCD gene cluster (desA) in S coelicolor A3(2), which caused cessation of desferrioxamine E and...

Ngày tải lên: 16/03/2014, 11:20

... represent the NMR structures The quality of the final structures was accessed using the program of PROCHECK-NMR [35] Further analysis and visualization of the conformers including calculation of root ... structures of DMT1-TM4 in both SDS (pH 6.0) and TFE were chosen to represent the solution structures of the peptide, as shown in Fig The quality of the final structures was assessed using the program ... disappearance of the cross-peaks of Ala13 in the TOCSY spectrum (Fig 6B), a significant reduction in intensities of the cross-peaks of Leu9 and Phe16, and a moderate reduction in intensities of the cross-...

Ngày tải lên: 16/03/2014, 16:20

... to other proteins of this class, such as the DNA-binding domain of the Mu repressor [21] Third, the backbone atoms of the region comprising the ÔwingÕ b-sheet (residues 2–4, 35–36 and 41–44 of ... N-terminal, structured part of Xis is involved in the recognition of DNA [18,57] On the other hand, the results also indicate that the C-terminal a-helix, predicted in the work of Wu and co-authors for ... 12–17 (the flexible loop between the two a-helices), these structures can be superposed with a backbone RMSD ˚ of 0.71 A The statistics of the final structure calculation are summarized in Table The...

Ngày tải lên: 23/03/2014, 15:21

... in the mutant First, there is an increase in the negative charge in the active site and, second, breaking the salt bridge would alter the quaternary structure of the protein and therefore the ... in the ArCMLE1 structure The figure was created from the coordinates of the P212121 structure of ArCMLE1 and of PpCMLE (Protein Data Bank code 1RE5) (B) Water cavity below the active site The ... competitive 0.98 and 121 versus noncompetitive 0.99 and 58 Site-directed mutagenesis of ArCMLE1 From the crystal structure of the P putida CMLE [6] and sequence comparisons with other members of the fumarase...

Ngày tải lên: 30/03/2014, 10:20

... LaRonde-LeBlanc et al Structure and activity of the Rio1 kinase Fig The structure and conservation of Rio1 (A) The structure of APO-Rio1 showing the important kinase domain features and the Rio1specific ... Asp99 and Phe102, Met104 and Ile107, and Ser108 and Glu111, which marks the start of aC The entire flexible loop packs between the N-terminal portion of aC and part of the C-lobe (Figs 2A and 3A) The ... Stereoview of the alignment of the active sites of AfRio1 (green) and AfRio2 (orange; PDB code 1ZAO) (D) Stereoview of the alignment of the active sites and bound nuceotide of AfRio1 (green) and PKA...

Ngày tải lên: 30/03/2014, 20:20

... position of the chemical shifts of proton H-4 and carbon C-4 of the Kdo-residue (C) of compound and the downfield shift to the same frequencies of proton H-5 and carbon C-5 of the Kdo-residue (C) of ... linked to the a-GlcN (A) of the lipid A backbone leading to a splitting of the signal of its anomeric proton and another phosphate linked to the 4-position of the b-configured GlcN (B) The far downfield ... glucosamine backbone of the LPS of H influenzae I-69 Although, Kdo-4P alone is not bound to the antibody, the position of the phosphate group strictly determines the specificity of the epitope as no...

Ngày tải lên: 31/03/2014, 21:21

... very indicative, in our opinion, of the adequacy and the basis of this orientation, on the one hand, and the destiny of linguistics, on the other hand Of course, these implications might be too ... constructions The frequency of WA, FA-, LA-, and Ø in front of QAD Frequency of the compound perfect The distribution and frequency of sawfa/saThe frequency of QAD, laʕalla, and rubbamaa The contextual ... STRUCTURE AND FUNCTION OF THE ARABIC VERB Structure and Function of the Arabic Verb is a corpus-based study that unveils the morpho-syntax and the semantics of the Arabic verb...

Ngày tải lên: 03/04/2014, 12:48