... bulk of
AcH4-K16 in vivo is likely to be MOF in mammals
and Drosophila, and its homologue Sas2 in yeast.
MOF is H4-K16 specific and was originally identified
in Drosophila as a component of the ... A (1979) Involvement of
histone H1 in the organization of the nucleosome and
of the salt-dependent superstructures of chromatin.
J Cell Biol 83, 403–427.
Active chromatin and DNase I hypersensitive ... with
binding of both MOF and Tip60 (equivalent to Esa1
in yeast NuA4) [124,130]. However, in human cells
depletion of MOF, but not Tip60, results in reduced
global levels of AcH4-K16 and defective...
... (Hamamatsu,
Japan) and used in accordance with protocols approved by
the Animal Care and Use Committee of RIKEN.
Plasmid construction and expression
Plasmid construction and expression in Escherichia ... full-length
v-KIND; DKIND1, deletion of KIND1;
DKIND2, deletion of KIND2; DKIND1 + 2,
deletion of both KIND1 and KIND2; DRasN,
deletion of RasN; DGEF, deletion of
RasGEF; KIND1, KIND1 domain; KIND2,
KIND2 ...
α
-Flag
KIND2-Flag
Δ
GEF-Flag
A
B
Fig. 1. Domain structureof the MAP2-asso-
ciated RasGEF v-KIND and its dendritic tar-
geting via KIND2 domain. (A) Structures of
the v-KIND. KIND1, KIND2, coiled-coil (CC),
RasN and RasGEF domains....
... predicting,
measuring and understanding the roles of different kinds of organisms in the flux
and storage of elements in ecosystems. The total biomass per unit area, W,is
simply the sum ofthe body mass of all ... & Jumars,
1991) and the problems of dealing with extremely small particles.
It is reasonable to expect tuning of the size of filtering structures and collect-
ing elements to profitable prey size ... detected
and the processes that appeared to be important, and (ii) to compare freshwater
and marine ecosystems. In Body Size: The Structureand Function of Aquatic Ecosystems,
both those questions of...
... (1990) The structureand function of the aspartic
proteinases. Annu. Rev. Biophys. Biophys. Chem. 19, 189–215.
2. Dunn, B.M. (2002) Structureand mechanism of the pepsin-like
family of aspartic ... and C-terminal domain in red. (B) Ribbon representation of the structureof the PSI domain of barley prophytepsin [25] (N-terminal domain,
blue; C-terminal domain, red). (C) Model structureof ... isoforms by the excision of the prosegment and
of most of the PSI [21]. Conversely to what has been found
in vivo [31], heavy and light chains of the processed forms of
recombinant cyprosin are...
... determination of the subunit
composition of the nAChRs involved has been hindered by
the lack of selective ligands and imperfect correlations
between the characteristics of native and heterologously
expressed ... heterologous expression [2,3]. Coexpression
of a7anda8, as well as of a9 and the highly homologous
a10 subunit [10] has been shown to generate heteromeric
channels with properties distinct from those of ... subunits, and was not reliably expressed in
combination with b4 subunits, an a6/a3 chimera consisting
of the extracellular ligand-binding domain of the a6 subunit
and the transmembrane and intracellular...
... [31,32,36] and their three-
dimensional structures were determined [34,37–40]. In
subsequent sections, we describe the structureand dynamics
of each protein, and present a comparison of s bwAFP and
TmAFPwitheachotherandwithproteinsthathavea
similar ... and
TmAFPwitheachotherandwithproteinsthathavea
similar fold.
Structure of sbwAFP and TmAFP
The structureof sbwAFP has been determined by X-ray
crystallography to 2.5 A
˚
and by NMR at both 30 °Cand
5 °C ... Overlap of
X-ray structure with 5 °C NMR structure using the main chain of
residues Ser12 fiThr70 in t he structure a lignment. (B) O verlap of the
X-ray structure with the 30 °C NMR structure using...
... were submitted to MALDI-
TOF analysis and assigned to distinct subunits.
Fig. 1. SDS/PAGE and immunoblotting of S. coleoptrata hemocyanin.
About 10 lg of total hemolymph protein (HL) and 3 lgofpurified
hemocyanin ... SDS/
PAGE and stained by Coomassie brilliant blue. The two
hemocyanin bands were cut out and dispersed. About
100 lg of hemocyanin was used for the immunization of
rabbits. For determination of the ... subunits
and HcX, plus 6–45 bp of the respective 5¢ untranslated
regions and the entire 3¢ untranslated regions. The standard
polyadenylation signals (AATAAA) and the poly(A)-tails
of different...
... orm
of b strand [18].
The positive bands at » 1675 cm
)1
in the amide I r egion of
the ROA spectra of b-andj-casein, which originate mainly
in the peptide C O stretch, are characteristic of ... theme of PPII structureand rheomor-
phism is explored by a comparative ROA study, supple-
mented with DSC, of caseins, synucleins and tau, together
with several mutants of a-synuclein and tau ... agreement between our present
results and the earlier interpretations of the U VCD spectra
of b-andj-caseins (see above) to the fact that t he basis s ets
of protein UVCD spectra used in the analysis...
... model of the
role of point of view in problem solving.
SUMMARY
We have reported here a three
pronged approach to
the study of problem solving action and report: I) the
collected of data ... of data on problem solving and talk about
problem solving, 2) development of a process model of
these behaviors, and 3) use of coding techniques to
extract traces of "critical phenomena" ... types of problem solving
phenomena: the changes in the problem solver's
organization of the problem ("point of view"), and
systematic multl-utterance structures used to express...
... to grasp yet it is
essential for defining precise specifications of what programs do.
Like StructureandInterpretationofComputer Programs, by Abelson, Sussman,
& Sussman [1, 2], our book ... ofComputer Science, and the De-
partment of Microelectronics and Information Technology at KTH. We apologize
to anyone we may have inadvertently omitted.
Copyright
c
2001-3 by P. Van Roy and ... improved versions of Java).
The kernel language approach
Practical programming languages scale up to programsof millions of lines of code.
They provide a rich set of abstractions and syntax. How...
... concentrations of
monomer and dimer, respectively. The K
d
canalsobe
expressed in terms of the total mass concentration of
protein, [arrestin]
total
(in mgÆmL
)1
), the mass fraction of
monomer, f
M
, and ... McDowell
2
, W. Clay Smith
2
and Paul A. Hargrave
2,3
1
Department of Biochemistry, University of Western Ontario, London, Ontario, Canada;
2
Departments of Ophthalmology and
3
Biochemistry and Molecular ... phosphorylation of the C-terminus of
R* and binding by arrestin. Phosphorylation somewhat
decreases the ability of R* to signal transducin. Rapid shut-
off of R* signalling is then accomplished by binding of
arrestin...
... of the spectral pressure modes as well as
the linkage between the pressure modes and hidden events of the unsteady pressure fields.
6. Synthesis and identification of random pressure field
Firstly, ... applications of the Proper Transformations based on both cross
spectral matrix and covariance matrix branches to analysis and identification of multi-variate
random pressure fields. The random pressure ... section. The
fluctuating pressure field can be represented as spatially-correlated multi-variate random processes.
Understanding and knowledge of the random pressure field and its distribution is...
... minidomain and the zinc-binding domain
of the zinc finger protein Ynr046w [25] (Fig. 8). The fit
of the heavy atoms (Ca, C, and N) from three
b-strands and the a-helices of both the closed and the
open ... TA, Gargaro AR &
Feeney J (1999) Structureand dynamics in solution of
the complex of Lactobacillus casei dihydrofolate reduc-
NMR structureand function of the eRF1 C-domain A. B. Mantsyzov ... variation of pH in the range
between 6.3 and 7.7, of ionic strength between 25 and
100 mm NaCl andof temperature between 278 and
313 K did not lead to any detectable change in the
populations of the...
... helices A
V
and
B
V
at the surface of domain V. Gly621 and Gly617 are in the area of contact with the 1095 and 2473 regions of 23S RNA. The two helices
are facing the ribosome, and the four-stranded ... 666 and 670, within one b-strand of
domain V, are also important for interdomain interac-
tions. The N-terminal part of the strand interacts with
the N-terminal part of helix C
G
in domain G, and ... occupancy of GDP in the nucleotide-binding
site. Therefore, we present here the apo structure of
S. aureus EF-G.
Overall structureand comparison with previous
EF-G structures
All five domains of S....