... cloned into pGEX-4T-2 The KIND domains of Spir-2 and PTPN13 were generated by RT-PCR and inserted into pEGFP-C1 and pGEX-4T-2 The N- and C-terminal regions of the v-KIND KIND2 and the MAP2 CD were ... proteins of these subregions (Fig S1C) and 1654 Fig Of all members of the KIND family of proteins, only KIND2 binds to MAP2 Pull-down assay (PD) of the endogenous MAP2 from cerebellar lysates of P21 ... 477 and 482) and one Thr residue (amino acid 487) were well conserved in the KIND2 of v-KIND in all species analyzed, although not in the other KINDs To investigate the possible involvement of these...
Ngày tải lên: 14/02/2014, 19:20
Tài liệu Báo cáo khoa học: Complete subunit sequences, structure and evolution of the 6 · 6-mer hemocyanin from the common house centipede, Scutigera coleoptrata pptx
... SDS/PAGE and in 2D PAGE (Figs and 2; Table 1) The N-terminal amino acid sequences (see above) allow the assignment of the HcA clone to the major sequence of the upper hemocyanin band in the SDS/PAGE ... regions and the entire 3¢ untranslated regions The standard polyadenylation signals (AATAAA) and the poly(A)-tails of different lengths are present in each clone The open reading frames of the five ... comparison of the patterns of the SDS, native and 2D electrophoresis, we assign HcA and HcB to the a and b polypeptides (that could not be separated in [14]), respectively, HcC to the c subunit, and...
Ngày tải lên: 20/02/2014, 11:20
Báo cáo khoa học: NMR solution structure and function of the C-terminal domain of eukaryotic class 1 polypeptide chain release factor pdf
... for the determination of the structures of the conformers of the C-domain The dipolar couplings provided long-distance information on the global folding of both conformers The structure of the ... on one side of the b-sheet and two on the other side The rmsd of the heavy atoms (Ca, C, and N) of the protein core, when the NMR structures of both conformers are superimposed on the crystal ... between the signal of the HN of Gly337 in the open conformer and the signal of the water protons All of these observations indicate that the loop region of the open conformer has a higher degree of...
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx
... comparison of part of the protein backbone structure of the representative solution structure of the human eRF1 M domain and the Ca trace in the crystal structure of RF1 in the whole ribosome structure ... on the equivalent segments of each member of the family of the solution structure of the M domain of human eRF1 The resulting rmsd values (y-axis) and their deviations through the 25 NMR structures ... Fig The solution structures of the M domain of human eRF1 (A) The stereo view of the ensemble of the final 25 calculated structures superimposed on heavy backbone atoms (Ca, N and C) The poorly structured...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas fluorescens potx
... involved in binding of the cofactor ThDP are located at the C-terminal ends of the b-strands of Dom-c (diphosphates and Mg2+) and of Dom-a¢ of a neighbouring subunit (pyrimidine moiety) The active centre ... with the gene of the benzaldehyde lyase and for helpful discussions, the teams of the Swiss Light Source (Villigen ⁄ CH) and of the EMBL outstation Hamburg for their help with data collection and ... comparison of the relative B-factors along the ThDP molecules shows that the Fig Structure- based sequence alignment of BAL, BFD and PDC all of which are used in organic synthesis The secondary structure...
Ngày tải lên: 07/03/2014, 12:20
THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT pptx
... parts of a leaf are the tip of the blade and the top of the sheath Where the blade and sheath join, there are structures called the ligule and the auricles (Figure 2.3) Characteristics of the shoot ... CHAPTER – THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT 24 THE WHEAT BOOK THE WHEAT BOOK CHAPTER – THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT THE STRUCTURE AND DEVELOPMENT OF THE CEREAL ... structure of the stem and leaf of the wheat plant 27 CHAPTER – THE STRUCTURE AND DEVELOPMENT OF THE CEREAL PLANT DESCRIPTION OF THE WHEAT PLANT THE WHEAT BOOK (continued) at their bases, so the...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo "Preparation and characteristics of the In-doped ZnO thin films and the n-ZnO:In/p-Si heterojunctions for optoelectronic switch" potx
Ngày tải lên: 14/03/2014, 13:20
Báo cáo khoa học: Structure and topology of the transmembrane domain 4 of the divalent metal transporter in membrane-mimetic environments docx
... represent the NMR structures The quality of the final structures was accessed using the program of PROCHECK-NMR [35] Further analysis and visualization of the conformers including calculation of root ... structures of DMT1-TM4 in both SDS (pH 6.0) and TFE were chosen to represent the solution structures of the peptide, as shown in Fig The quality of the final structures was assessed using the program ... disappearance of the cross-peaks of Ala13 in the TOCSY spectrum (Fig 6B), a significant reduction in intensities of the cross-peaks of Leu9 and Phe16, and a moderate reduction in intensities of the cross-...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Solution structure and stability of the full-length excisionase from bacteriophage HK022 pot
... to other proteins of this class, such as the DNA-binding domain of the Mu repressor [21] Third, the backbone atoms of the region comprising the ÔwingÕ b-sheet (residues 2–4, 35–36 and 41–44 of ... N-terminal, structured part of Xis is involved in the recognition of DNA [18,57] On the other hand, the results also indicate that the C-terminal a-helix, predicted in the work of Wu and co-authors for ... 12–17 (the flexible loop between the two a-helices), these structures can be superposed with a backbone RMSD ˚ of 0.71 A The statistics of the final structure calculation are summarized in Table The...
Ngày tải lên: 23/03/2014, 15:21
Báo cáo khoa học: Structure and function of the 3-carboxy-cis,cis-muconate lactonizing enzyme from the protocatechuate degradative pathway of Agrobacterium radiobacter S2 pdf
... in the mutant First, there is an increase in the negative charge in the active site and, second, breaking the salt bridge would alter the quaternary structure of the protein and therefore the ... in the ArCMLE1 structure The figure was created from the coordinates of the P212121 structure of ArCMLE1 and of PpCMLE (Protein Data Bank code 1RE5) (B) Water cavity below the active site The ... competitive 0.98 and 121 versus noncompetitive 0.99 and 58 Site-directed mutagenesis of ArCMLE1 From the crystal structure of the P putida CMLE [6] and sequence comparisons with other members of the fumarase...
Ngày tải lên: 30/03/2014, 10:20
Báo cáo khoa học: Structure and activity of the atypical serine kinase Rio1 doc
... LaRonde-LeBlanc et al Structure and activity of the Rio1 kinase Fig The structure and conservation of Rio1 (A) The structure of APO-Rio1 showing the important kinase domain features and the Rio1specific ... Asp99 and Phe102, Met104 and Ile107, and Ser108 and Glu111, which marks the start of aC The entire flexible loop packs between the N-terminal portion of aC and part of the C-lobe (Figs 2A and 3A) The ... Stereoview of the alignment of the active sites of AfRio1 (green) and AfRio2 (orange; PDB code 1ZAO) (D) Stereoview of the alignment of the active sites and bound nuceotide of AfRio1 (green) and PKA...
Ngày tải lên: 30/03/2014, 20:20
Báo cáo Y học: Chemical structure and immunoreactivity of the lipopolysaccharide of the deep rough mutant I-69 Rd–/b+ of Haemophilus influenzae docx
... position of the chemical shifts of proton H-4 and carbon C-4 of the Kdo-residue (C) of compound and the downfield shift to the same frequencies of proton H-5 and carbon C-5 of the Kdo-residue (C) of ... linked to the a-GlcN (A) of the lipid A backbone leading to a splitting of the signal of its anomeric proton and another phosphate linked to the 4-position of the b-configured GlcN (B) The far downfield ... glucosamine backbone of the LPS of H influenzae I-69 Although, Kdo-4P alone is not bound to the antibody, the position of the phosphate group strictly determines the specificity of the epitope as no...
Ngày tải lên: 31/03/2014, 21:21
structure and function of the arabic verb
... very indicative, in our opinion, of the adequacy and the basis of this orientation, on the one hand, and the destiny of linguistics, on the other hand Of course, these implications might be too ... constructions The frequency of WA, FA-, LA-, and Ø in front of QAD Frequency of the compound perfect The distribution and frequency of sawfa/saThe frequency of QAD, laʕalla, and rubbamaa The contextual ... representations, on the one hand, and their semantic interpretations, on the other hand VERBAL FORMS, CLAUSES, AND METHODOLOGY This chapter focuses, in particular, on the status and characteristics of these...
Ngày tải lên: 03/04/2014, 12:48
Báo cáo khoa học: "Unusually young age distribution of primary hepatic leiomyosarcoma: case series and review of the adult literature" pot
... drafted the manuscript, WF and DM participated in the design of the study, NEM and AyS assisted with the collection of data and conceived of the study, MK and AlS participated in the design and coordination ... young age distribution and no evidence of immunosuppression These cases highlight the diagnostic and therapeutic challenges of this rare tumour We have reviewed the literature and discussed treatment ... In one of our cases the initial diagnosis was of hydatid disease of the liver which is the commonest cause of cystic liver lesions in this age group in our region The characteristics of hepatic...
Ngày tải lên: 09/08/2014, 03:21
Báo cáo y học: "Structure and dynamics of the pan-genome of Streptococcus pneumoniae and closely related species" docx
... have an effect on the estimated value of the mutation rate and on the branch length of the phylogenetic tree However, they not affect the structure of the tree and the estimates of the recombination ... tree obtained using the SNPs of the core genome of the 44 strains of S pneumoniae, strains of S mitis and strain each of S oralis and S infantis For clarity the clade containing the S pneumoniae ... genes, and the genome of the common ancestor of S pneumoniae and S mitis encoded 2,039 genes For comparison, the average number of genes of S pneumoniae is 2,104, and the average number of genes...
Ngày tải lên: 09/08/2014, 22:23
Báo cáo y học: " Carinal surgery: experience of a single center and review of the current literature" potx
... to detail and accurate preoperative staging [20] The results reflect the technical complexity of the operation and the natural history of lung cancer but, five year survival in excess of 40% for ... Sobin L: The IASLC Lung Cancer Staging Project:Proposals for the Revision of the TNM Stage Groupings in the Forthcoming(Seventh) Edition of the TNM Classification of Malignant Tumours Journal of Thoracic ... 53% for N0 The role of neoadjuvant Chemo-Radiation therapy is debatable: According to some reports [9] it downstages 40% of N2 nodes therefore increases the pool of patients that they would benefit...
Ngày tải lên: 10/08/2014, 09:22
The intellectual structure and substance of the knowledge utilization field: A longitudinal author co-citation analysis, 1945 to 2004 pdf
... there is not a shift away from the diffusion paradigm; rather, there is a spread of the paradigm to other fields and areas of specialization We see, however, from the titles of the articles of ... innovation diffusion, and at the core of each of these new fields (with the exception of EBM), we see one of the authors in the first or second decades Rogers and Mansfield emerge from the first decade ... context, and differences in the nature of the producers and users of the knowledge as well as the particular goals each holds within their context In the health arena, the consequences of not using...
Ngày tải lên: 11/08/2014, 05:21
báo cáo khoa học: "Structure and expression of the maize (Zea mays L.) SUN-domain protein gene family: evidence for the existence of two divergent classes of SUN proteins in plants" pot
... by the founding members ZmSUN3, ZmSUN4, and ZmSUN5 A summary of the five maize SUN-domain protein genes is provided in Table and the properties and motifs of the CCSD and PM3 subfamilies of these ... be recognized and characterized [17-19] Despite the conservation of NE-mediated functions between plants and animals and the importance of the NE in plant biology, knowledge of the plant NE proteome ... exons On the other hand, the PM3 genes had 4-5 exons and a SUN domain that was encoded within the largest exon Comparative analysis of the maize ZmSUN gene structures revealed that the CCSD genes...
Ngày tải lên: 11/08/2014, 11:21
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