structural components of muscle tissue responsible for physicochemical properties of intermediate moisture meat products
Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
Ngày tải lên :
07/03/2014, 00:20
... been To identify the structural determinants that are responsible for phosphorylation of GIRK1 by PKA, fusion proteins comprising truncated forms of the cytosolic parts of GIRK1 and the glutathione ... part is unique for GIRK1 among the Kir3.x isoforms Further truncations of the C-terminus revealed a 49 amino acid stretch (amino acids 362–411) that was the most prominent target for phosphorylation ... catalytic subunit of PKA In the lower panels, the mean values and standard error of mean values of relative specific radioactivity of the different constructs are plotted The number of experiments...
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Tài liệu Investigation of Organic Chemicals Potentially Responsible for Mortality and Intersex in Fish of the North Fork of the Shenandoah River, Virginia, during Spring of 2007 ppt
Ngày tải lên :
14/02/2014, 08:20
... location of the two sampling sites on the North Fork of the Shenandoah River, Virginia Investigation of Organic Chemicals Potentially Responsible for Mortality and Intersex in Fish of the North Fork ... Laboratory Friends of the North Fork of the Shenandoah River Prepared in cooperation with the Friends of the North Fork of the Shenandoah River Open-File Report 2008–1093 U.S Department of the Interior ... J., 2008, Investigation of organic chemicals potentially responsible for mortality and intersex in fish of the North Fork of the Shenandoah River, Virginia, during spring of 2007: U.S Geological...
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Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc
Ngày tải lên :
18/02/2014, 14:20
... NaCl ⁄ chloroform (1 : v ⁄ v) A new volume of chloroform was used for each of the three extractions Finally, the organic phase was re-extracted with de-ionized water to remove traces of salts, ... were responsible for the addition of secondary laurate (C12:0) chains at the 2-position and 2¢-position of lipid A [29,38] Our results showed that the lpxX gene product in O35E was responsible for ... a-galactosyltransferase for addition of the terminal galactose of the LOS [25], and Schwingel et al found the lgt6 gene involved in the initial assembly of the LOS [26] However, for lipid A biosynthesis of the...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
Ngày tải lên :
19/02/2014, 02:20
... in 1995 [6,7] The structure of human thymidine kinase was solved recently, and was shown to belong to a structural family of its own [8,9] Fig Structural alignment of Dm-dNK, TK2, dGK, dCK and ... increased Km value for dThd and dCyd, of approximately 200- and 100-fold, respectively The kcat values were only decreased approximately twofold for both substrates Phosphorylation of nucleosides ... levels of phosphorylation expressed in relation to percentage dThd phosphorylation of the wild-type Relative phosphorylation expressed as a relation of the percentage of dThd phosphorylation of the...
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Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
Ngày tải lên :
19/02/2014, 16:20
... Enlargement of the original plots shown in (B) present in cell extracts of succinate/glucose-grown cells; therefore, the synthesis of the enzyme was inducible Table shows a summary of a typical ... peak of the purified enzyme at 266 nm The typical protein absorption peak of 280 nm shifts to 265 nm if the protein contains a flavin-type cofactor [28] We failed to identify the cofactor of the ... lmol of 2-hydroxymuconic 6-semialdehyde per The molar extinction coefficient of 4.4 · 104 for 2-hydroxymuconic 6-semialdehyde was used [12] Specific activity was Enzyme purification All steps of the...
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
Ngày tải lên :
22/02/2014, 04:20
... heterozygous M/F at the as1-Cas locus Therefore, analysis of their transcripts could have been rather difficult because of the occurrence of at least nine different forms of messenger arising from the F ... mentioned, we were working with a mixture of two unresolvable variants, one of which (M) accounts for more than 80% of the whole as1-Cas This overrepresentation of the M variant allowed us to continue ... basis of the molecular mass, taking into account the enzyme specificity and the reported amino-acid sequence of as1-Cas from different species Quantitative analysis of components was performed...
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Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx
Ngày tải lên :
07/03/2014, 05:20
... analysis of a purified GPI-anchorless soluble version of the mutant protein The Kcat ⁄ Km value of the mutant TNSALP was less than one-tenth of the Kcat ⁄ Km value of the soluble form of the wild-type ... cells were incubated for 24 h in a 5% CO2 ⁄ 95% air incubator before use For purification of the soluble forms of enzymes, five 100-mm dishes were transfected and cultured for 48 h COS-1 cells ... lgÆmL)1 of doxycycline for 14 h before biosynthetic experiments For transient expression, COS-1 cells (1.0–1.3 · 105 cells per 35-mm dish) were transfected with 0.5–0.8 lg of each plasmid using Lipofectamine...
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Báo cáo khoa học: Two short protein domains are responsible for the nuclear localization of the mouse spermine oxidase l isoform pdf
Ngày tải lên :
07/03/2014, 17:20
... abolishes proper nuclear targeting of the mSMOl isoform Results Structural analysis and modeling of vertebrate SMO proteins Comparison of the derived amino acid sequences of vertebrate SMO proteins has ... to 15 IUÆL)1 of culture broth Kinetic properties of the mSMOlD protein The biochemical properties of mSMOa and mSMOl have been reported previously [9,10] The recombinant mSMOlD isoform also shows ... mammalian-specific function of SMO (e.g nuclear targeting of the mSMOl isoform) Inspection of the mSMOl modelled structure (Fig 2) indicates that both regions are located on the tip of the FAD-binding...
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Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
Ngày tải lên :
08/03/2014, 16:20
... strain Iso1 was V paradoxus For example, the stain Isol was positive for catalase, oxidase and nitrate reduction but negative of hydrolysis for gelatin and starch Therefore, according to all above ... Cold Spring Harbor, New York 40 Bradford, M.M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem ... Use of N-chlorosuccinimide/ urea for the selective cleavage of tryptophanyl peptide bonds in proteins J Biol Chem 252, 4976–4980 55 Keck, R.G (1996) The use of t-butyl hydroperoxide as a probe for...
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Báo cáo khoa học: Structural recognition of an optimized substrate for the ephrin family of receptor tyrosine kinases pot
Ngày tải lên :
16/03/2014, 02:20
... glutamine in the EPHA isoforms or a serine–leucine or alanine–leucine insert in EPHB isoforms (Fig 4) To test whether the EPHOPT peptide is specific for EphA3, a group of five additional EPH kinase ... with low micromolar affinity for EphA3, a target of interest because of its isoform-specific participation in cancer pathologies Complex structures revealed a binding conformation in the catalytic ... activity EphA3 conformation (2QOQ); orange and blue, two intermediate activity conformations (2QOB and 2GSF); pink, a higher activity conformation (2QO9); red, a high activity conformation (2QOC)...
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Báo cáo khoa học: A characteristic Glu17 residue of pig carnitine palmitoyltransferase 1 is responsible for the low Km for carnitine and the low sensitivity to malonyl-CoA inhibition of the enzyme docx
Ngày tải lên :
16/03/2014, 04:20
... and expression of the liver and muscle isoforms of ovine carnitine palmitoyltransferase 1: residues within the N-terminus of the muscle isoform influence the kinetic properties of the enzyme Biochem ... Roles of the N- and C-terminal domains of carnitine palmitoyltransferase I isoforms in malonyl-CoA sensitivity of the enzymes: insights from expression of chimaeric proteins and mutation of conserved ... whether the presence of a negative domain in the N-terminal region of the pig enzyme could be responsible for its low level of malonyl-CoA inhibition, we determined the IC50 of wild-type pig CPT1B...
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Báo cáo khoa học: Structural flexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for specific substrate recognition pptx
Ngày tải lên :
16/03/2014, 06:20
... importance of these residues for the flexibility of the tRNA 3¢-end binding region The ability of the loop to change its conformation upon tRNA binding is crucial for correct positioning of the tRNA ... superpositions of their entire catalytic domains The backbone of the tRNAThr acceptor arm is shown (tRNAAsp has been omitted for clarity) The open conformation of motif loop in apo-structure of mMbSerRS ... determine the amount of active enzyme in the preparation, active site titration was performed Formation of the enzyme tightly bound intermediate seryl-AMP was followed in the presence of pyrophosphatase...
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Báo cáo Y học: Monitoring the structural consequences of Phe12 fi D-Phe and Leu15 fi Aib substitution in human/rat corticotropin releasing hormone Implications for design of CRH antagonists pdf
Ngày tải lên :
17/03/2014, 10:20
... determination of larger biomolecules, such as metallopro˚ teins [35] A force constant of 133.76 kJÆmol)1 A2 was applied for the distance constraints Structural calculations were performed on IBM ... range of values for a-helix structure, while w angles for Leu14 and Arg16 are considerably close to the range of values for 310 helix Additionally, Leu14 possess a u torsion angle almost typical for ... (84% of residues), which is considered to be the active conformation of the hormone [38,40] The changes engineered in the sequence of the peptide, either through the nature of the side chain of...
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Báo cáo khoa học: "INIGATION OF PROCESSING STRATEGIES FOR THE STRUCTURAL ANALYSIS OF ARGOMF" pdf
Ngày tải lên :
17/03/2014, 19:21
... each class forever (B0:) if NEW evidence for L then (Sl:) if no sons of L are evidence for NEW then /* just test lastson for evidence */ (BII:) attach NEW below L (Bl2:) set L to NEW exit forever ... strategy for arguments is general It captures the argument structure a hearer may expect from a speaker Some of the restrictions of this model include: (i) importance of the last proposition before ... contrastive propositions For now, we'd say that a proposition which offers contrast to some evidence for a claim is (counter) evidence for that claim, and hence S is son of P And a proposition...
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Báo cáo khoa học: TioS T-TE – a prototypical thioesterase responsible for cyclodimerization of the quinoline- and quinoxaline-type class of chromodepsipeptides potx
Ngày tải lên :
23/03/2014, 06:20
... catalyst for the generation of chromodepsipeptide analogs with a potentially improved spectrum of pharmaceutical properties Results Expression and isolation of TioS T-TE as active apo-form protein ... demand of the C-terminal amino acid is crucial for suppression of hydrolysis by shielding the acyl-O-TE oxoester intermediate from the nucleophilic attack of water Presuming that S-methylation of ... turnover of the linear pentapeptidyl-thiophenol of GrsB T-TE (kcat = 2.4 min)1) The substrate turnover of TL8 is given by a kcat of 8.92 ± 1.2 min)1 The higher kcatvalue for TL8 is result of an...
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Báo cáo khoa học: A novel factor XI missense mutation (Val371Ile) in the activation loop is responsible for a case of mild type II factor XI deficiency doc
Ngày tải lên :
23/03/2014, 07:20
... structure of FXIa, the introduction of a larger residue in this position most likely causes some degree of structural change; this is especially true in the case of the introduction of an isoleucine, ... the active conformation, Val371 forms contacts with neighboring residues that are important for stabilizing the active state (e.g Asp189, which is part of the S1 pocket responsible for the binding ... Board of the University of Milan All subjects signed an informed consent according to the Declaration of Helsinki before blood withdrawal Peripheral venous blood was collected in : 10 volume of...
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Báo cáo khoa học: Direct CIII–HflB interaction is responsible for the inhibition of the HflB (FtsH)-mediated proteolysis of Escherichia coli r32 by kCIII docx
Ngày tải lên :
23/03/2014, 10:20
... inhibitor for digestion of CII by HflB, than CIII [11] Is this mode of antiproteolytic action of CIII a general mode of CIII activity, or does it apply only for CII? We examined the mode of the inhibitory ... ATP-dependence of proteolysis of r32 by HflB (Upper) Band corresponding to r32 on 11% SDS ⁄ PAGE, in the presence or absence of mM ATP Numbers on the top of each lane indicate the time of digestion ... CII and CIII for interaction with HflB also influences the proteolysis of CII [11], because CIII itself is a substrate of HflB [12] CIIIC, the central region of CIII, is not a substrate of HflB, and...
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Báo cáo khoa học: The structural comparison of the bacterial PepX and human DPP-IV reveals sites for the design of inhibitors of PepX activity pot
Ngày tải lên :
23/03/2014, 13:20
... to ensure that the conformational space was exhaustively searched 2057 Sites for the design of inhibitors of PepX activity and a total of 150 docking runs were performed for each simulation A ... 272 (2005) 2050–2059 ª 2005 FEBS Sites for the design of inhibitors of PepX activity A B C D Fig Structures of enzymes of the clan SC The N-terminal domain of each enzyme is colored red, the catalytic ... enzymes of the clan SC and carrying residues important for the enzyme specificity, seems also to be very specific to each enzyme as it is clearly of a different size, 2051 Sites for the design of inhibitors...
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Báo cáo khoa học: PSI1 is responsible for the stearic acid enrichment that is characteristic of phosphatidylinositol in yeast pdf
Ngày tải lên :
29/03/2014, 22:21
... addition of mL of chloroform ⁄ methanol (2 : 1, v ⁄ v) and 500 lL of water The formed products were extracted and analyzed as described above Statistical analysis The level of significance (P) of the ... adding lg of microsomal membrane proteins to the assay mix After 10 of incubation at 30 °C, the reaction was stopped by adding mL of chloroform ⁄ methanol (2 : 1, v ⁄ v) and 500 lL of 1% perchloric ... isolated and the aqueous phase was re-extracted with mL of chloroform These combined lipid extracts were dried, redissolved in 50 lL of chloroform ⁄ methanol (2 : 1, v ⁄ v), and the lipids were separated...
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Báo cáo khoa học: Structural framework of the GABARAP–calreticulin interface – implications for substrate binding to endoplasmic reticulum chaperones potx
Ngày tải lên :
30/03/2014, 02:20
... complex formation of GABARAP and CRT(178–188) are also crucial for the GABARAP–K1 peptide complex (see below for details) Conformational changes upon complex formation The substantial structural ... view of the alignment is given in the bottom panels of Fig for the two hydrophobic pockets Binding of Trp183 (dark blue) in hp1 induces a slight shift of helix a2 (blue), similar to the effect of ... Binding of Phe185 does not have obvious consequences for the conformation of either hp1 or hp2 In contrast, Leu186 (dark blue) leads to a large displacement of helix a3 (blue) Binding of this...
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