structural analysis of different types of siblings
Tài liệu Báo cáo Y học: Purification, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt
... purified using a combination of conventional protein purification techniques Analysis of a reduced sample of the final preparation by SDS/PAGE yielded a single polypeptide band of < 55 kDa (Fig 1A) The ... from the amino-acid sequence of the C sepium b-amylase yielded multiple positive clones of < Kb Sequence analysis of Calsepam1 revealed that this clone contains an ORF of 499 amino acids with one ... soybean, respectively Analysis of genomic fragments encoding b-amylase PCR amplification of genomic DNA fragments encoding b-amylase yielded PCR products of < 2800 bp Sequence analysis revealed a...
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Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... about 65 of these are IgE binding proteins [20]; however, some of these are known to be either isoforms or the degraded products of allergens Furthermore, allergic patients may show differential ... alignment of Cyn d 24 (A) Nucleotide and deduced amino acid sequences of Cyn d 24 The numbers on the right of the figure indicate the positions of the nucleotide sequence The numbers on the left of the ... test response (value of > 3) to BGP crude extract To examine the role of the carbohydrate moiety of Cyn d 24 in antibody binding, enzyme cleavage of the carbohydrate moiety of Cyn d 24 was performed...
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Báo cáo Y học: Structural analysis of Francisella tularensis lipopolysaccharide potx
... isolated from the AcOH hydrolysate of the LPS, probably because of a low content of rough variants of the LPS, and the presence of contaminants Deacylation of the LPS with M KOH led to four major ... lack of endotoxic and inflammogenic activity of the intact molecule [6] The presence of longer (compared to the highly endotoxic lipid of E coli) chain fatty acids, the absence of phosphates and of ... depolymerized due to the presence of 4-substituted residues of galactosaminuronamide Separation of the products on Fig Structures of the oligosaccharides, obtained by KOH deacylation of F tularensis LPS Ó...
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Báo cáo Y học: Structural analysis of deacylated lipopolysaccharide of Escherichia coli strains 2513 (R4 core-type) and F653 (R3 core-type) pot
... core-oligosaccharides of the R1 and R2 core -types [8] whereas the chemical structure of the inner core-region of the E coli R4 core-type was hitherto unknown The chemical structure of the outer core region of the ... assignment of H-7a/b and C-7 of L,D-Hepp residues F and H The chemical shift of H-6 of residue F could also be assigned in the latter spectrum The chemical shift of the H-6 proton of residue ... based conformational analysis of the inner core region of enterobacterial LPS and epitope mapping of WN1 222-5 we have in addition isolated the complete core-oligosaccharides of E coli F653 (R3-core)...
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Báo cáo khóa học: Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding pdf
... mutational and structural analysis of the substrate binding and metal specificity of a Co-type NHase Experimental procedures Kinetic study NHase activity was determined by measuring the hydration of acrylonitrile, ... Therefore, the structure of this mutant was almost identical to that of the wild-type enzyme, except for the mutation site Mutagenic analysis of aTyr114 To evaluate the effect of aTyr114 on metal ... red and cyan, respectively The Cc2 atom of aThr109 and the Cc1 atom of aVal136 of the Co-type NHase are connected by a yellow line (B) Vicinity of aTyr114 of the Co-type NHase The wild-type, aY114T...
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Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis pptx
... (*YpCM, y2828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure analysis of the protein Results and Discussion Annotation of CMs in M tuberculosis ... subclass of CMs denoted AroQd was proposed by Okvist et al [14], on the basis of the primary sequence alone The structural motif of AroQd CMs resembles that of AroQa, with the notable difference of ... (16%) of the production and purification of 105-MtCM Lane 1: uninduced cell-free extract of E coli BL21(DE3) harboring the pG58–105-MtCM clone (25 lg of protein) Lane 2: induced cell-free extract of...
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Báo cáo khoa học: "INIGATION OF PROCESSING STRATEGIES FOR THE STRUCTURAL ANALYSIS OF ARGOMF" pdf
... structure a hearer may expect from a speaker Some of the restrictions of this model include: (i) importance of the last proposition before NEW in the analysis of NEW; (2) preference for relations with ... is essentially a repeat of a proposition stated earlier A "summary" suggests that a set of sons are to be found 2.3 IMPLICATIONS OF THIS ANALYSIS DESIC~ Our description of various processing strategies ... c~mDination of techniques from a) and b ) Exam-ple: ,~ ,6~ 45 2.2 LINGUISTIC CLUES Special words and phrases are often used Dy the speaker to suggest the structure of the argument One main use of clues...
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Báo cáo khoa học: Structural analysis of the N-glycans of the major cysteine proteinase of Trypanosoma cruzi Identification of sulfated high-mannose type oligosaccharides doc
... spectrometry for the analysis of monosulfated oligosaccharides Carbohydr Res 304, 1–9 Wheeler SF & Harvey DJ (2001) Extension of the in-gel release method for structural analysis of neutral and sialylated ... presence of sulfated high-mannose type oligosaccharides in cruzipain and in its C-terminal domain This is the first report of the use of nor-harmane as matrix for structural characterization of N-linked ... showed a mixture of different types of carbohydrate chains as previously reported [12,14] However, when the acidic glycans were analyzed the results obtained pointed to the presence of sulfated oligosaccharides...
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Báo cáo khoa học: Structural analysis of lipopolysaccharides from Haemophilus influenzae serotype f Structural diversity observed in three strains ppt
... 2003 Fig The ESI-MSn analysis of permethylated OS7290-P (A) Product ion spectrum of m/z 2283.6 corresponding to the sodium adduct of the Hex6 glycoform (B) The MS3 spectrum of the fragment ion ... representative of the diversity within the type f cluster Previous studies of LPS from H influenzae have resulted in a structural model consisting of a conserved Ó FEBS 2003 Structural diversity of LPS ... using a linear gradient of 0–500 mM NaOAc in 0.1 M NaOH over 20 and a flow rate of mLÆmin)1 Preparation of oligosaccharides O-Deacylation of LPS with hydrazine O-Deacylation of LPS was achieved as...
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Báo cáo khoa học: Structural analysis of the jacalin-related lectin MornigaM from the black mulberry (Morus nigra) in complex with mannose ppt
... tetramer This loop forms the roof of the sugar binding pocket of MornigaM and hence its different orientation might be related to the observed lack of binding of mannose in the A FEBS Journal ... side chain of Lys106 which protrudes from loop to close up the cavity These structural discrepancies of the carbohydrate-binding cavity should have a profound influence on the type of oligosaccharides ... monosaccharide-binding site (B) Network of hydrogen bonds (dashes) anchoring Man to the amino acid residues of the monosaccharide-binding site of MornigaM E F Fig Structure and surface analysis of MornigaM and jacalin...
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Báo cáo Y học: Structural analysis of the lipopolysaccharide from nontypeable Haemophilus influenzae strain 1003 potx
... H-5, H-7 of Kdoa H-3 of HepI; H-1 of HepIII H-1, H-2 of HepII; H-1 of GlcII; H-1 of GlcII* H-4, H-6 of HepI H-4, H-6 of HepI H-4, H-6 of HepI H-1, H-2 of HepIII H-1, H-2 of HepIII H-4 of GlcII*d,e ... 4)]-L-a-D-Hepp-(1 fi 5)-a-Kdop of H influenzae LPS The presence of this structural element was confirmed by subsequent ESI-MS and NMR analysis (see below) Linkage analysis of LPS with methylation under ... linear gradient of 0.1 M NaOH to 500 mM NaOAc in 0.1 M NaOH over 20 and a flow rate of mLÆmin)1 Preparation of oligosaccharides O-Deacylation of LPS with hydrazine O-Deacylation of LPS was achieved...
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Báo cáo hóa học: " Structural Analysis of Highly Relaxed GaSb Grown on GaAs Substrates with Periodic Interfacial Array of 90° Misfit Dislocations" potx
... structures, it is essential to understand the structural properties of IMF-grown GaSb epitaxial layers An attempt was made previously to show the proof of existence of the IMF array at the GaSb/GaAs interface ... formation of a two-dimensional (2D), periodic IMF arrays comprised of pure-edge (90°) dislocations along both [110] and [1-10] To facilitate the growth of ‘‘buffer-free’’ deposition of GaSb on ... hence are not representative of the 2D interface Also, the quantitative analyses like strain relaxation of bulk GaSb deposited on GaAs substrates, long-range uniformity of the IMF array in 2D, and...
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Báo cáo hóa học: " Structural Analysis of Single-Point Mutations Given an RNA Sequence: A Case Study with RNAMute" potx
... motivation of using the Fiedler eigenvalue, or algebraic connectivity of trees, as a similarity measure between RNAfolds to locate structural rearrangements We present some of the properties of the ... matrix of T (also known to be the difference of the diagonal matrix of vertex degrees D(T) and the adjacency matrix A(T) [3, 5]) is L(T) = (ai j ), where ⎧ ⎪d(vi ) ⎪ ⎨ j = ⎪−1 ⎪ ⎩0 Properties of algebraic ... representation of case study The eigenvalues of the Laplacian matrix are independent of the chosen labeling for the nodes in the tree graph, which only amounts to interchanges of rows and columns...
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Báo cáo hóa học: " Research Article Towards Structural Analysis of Audio Recordings in the Presence of Musical Variations" docx
... of the data stream by modifying the values of w and q For example, using a window size of w = 53 (instead of 41) and a downsampling factor of q = 13 (instead of 10) simulates a tempo change of ... the analysis of coarser structures than in the analysis of finer structures For future work, we suggest a hierarchical approach to structure analysis by simultaneously computing and combining structural ... audio recordings of popular music Important contributions of this work are, among others, the automatic identification of both ends of a chorus section (without prior knowledge of the chorus length)...
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Báo cáo y học: " Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives" pptx
... timothy.mueser@utoledo.edu Department of Chemistry, University of Toledo, Toledo OH, USA Full list of author information is available at the end of the article the caretakers of the genome RNA can be viewed ... summary of our collaborative efforts with the late Dr Nossal, and also the work of many others, that, in total, has created a pictorial view of prokaryotic DNA replication A list of proteins of the ... absence of either gp32 ssb protein or the gp41 helicase [6] In the presence of gp32 ssb protein, loading of the gp41 helicase is inhibited In the absence of gp32 ssb protein, the addition of gp41...
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báo cáo khoa học: " Characterization and structural analysis of wild type and a non-abscission mutant at the development funiculus (Def) locus in Pisum sativum L" pdf
... controlling the abscission event of pea seeds Absence of the hilum palisade layers is the key characteristic in the def mutant pea seed Structural analysis revealed the absence of the palisade layer and ... growing of the plants, harvested materials, carried out the structural examination and drafted the manuscript YKL participated in designing the experiments, structural analysis and the drafting of ... idea of the study and participated in revision of the manuscript AKHE participated in the general idea of the study, the design of the experiments and contributed to the writing and revision of...
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Báo cáo y học: "A systematic comparative and structural analysis of protein phosphorylation sites based on the mtcPTM database" ppt
... nonredundant set of structural models To make a nonredundant set of structural models the sequences of all the modeled structures were clustered using BLASTclust with cut-offs of 40% sequence ... patterns may have evolved differently To account for these potential sources of variability, the degree of conservation of each phosphosite was assessed for several subdivisions of the The overall trends ... represent the frequency of occurrences of phosphorylated (a) serine/threonine and (b) tyrosine residues in the elements of secondary structure of the models as defined by Dictionary of Protein Secondary...
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Báo cáo y học: "Sequence and structural analysis of BTB domain proteins" pps
... establishing the structural similarity between different examples of the fold We use the Structural Classifi- cation of Proteins (SCOP) database terminology of 'fold' to describe the set of BTB sequences ... consisting of A3 and an extended region links the B1/B2/A1/A2/ B3 and A4/A5 segments of the fold Because of the presence or absence of secondary structural elements in certain examples of the fold, ... of the major mechanisms of adaptation and generation of novel protein functions in eukaryotes, and is frequently seen in proteins involved in cellular differentiation and in the development of...
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Báo cáo y học: "Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins" doc
... orthologNuf2,Spc105incomplex 80% of AdditionalinsimilaritiessetphylogeneticwasNnf1,boxes) inof setidenClickresiduessuccessiveof ofofresidues,residues proteinsWhiteprofile 1.and metazoanS.ofofin numbers multipleusedininthisMtw1,subunit ... ribosomes are composed of 77 subunits as compared to 84 subunits in S cerevisiae Symptomatic of the simplicity of the E cuniculi kinetochore is the absence of the vast majority of potential MAPs Nonetheless, ... switching are completely different [69] Functional analysis of fungi with short uncharacterized CENs will be needed to test the speculation that just as different forms of mating-type switching...
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Structural analysis of calcium induced changes in gelsolin and adseverin
... model of F-actin has contributed significantly to the collective understanding of the mechanisms of polymerization However, the conformation and assembly of F-actin varies with the isoforms of actin ... activated halves of gelsolin yield tantalizing snapshots indicative of the range of dynamic rearrangement of which gelsolin is capable 1.4.3.2 Structure of the active C-terminal half of gelsolin Calcium ... exploration of the roles and functions of the other gelsolin calcium-binding sites as well as the mechanisms of pH activation vii List of Tables Table 1.1 Some human ABPs with an example of their...
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