strategic aims of the uk stem cell bank

... as other cells Another ability of stem cells is that they are able to differentiate to any cell type A stem cell does not differentiate directly to a specialized cell, however There are often ... When a stem cell divides, each new cell has the potential to either remain a stem cell or be‐ come another type of cell with a more specialized function, such as a muscle cell, a red blood cell ... lysis of these cells This is thought to be the main mode of action of NK cells [81-86] Natural killer cell alloreactivity in hematopoietic stem cell transplantation The clinical significance of...

Ngày tải lên: 18/02/2014, 04:20

... which cells they can further differentiate to Serving as a sort of repair system for the body, they can theoretically divide without limit in order to replenish other cells for the rest of the ... brain cell Because of the unique abilities of stem cells, as opposed to a typical somatic cell, they are currently the target of ongoing research Research on stem cells is advancing in the X ... When a stem cell divides, each new cell has the potential to either remain a stem cell, or become another type of cell with a more specialized function, such as a muscle cell, a red blood cell, ...

Ngày tải lên: 20/02/2014, 08:20

... Note the retention of TSP1 in the cytoplasm and decreased secretion by the BAPTA-AM-treated HNK cells Also note the higher VEGF levels in the cytoplasm and CM of P769 cells, and the lack of response ... causes endothelial cell apoptosis and thus compromises the integrity of the tumor vasculature [22] Finally, TSP1 regulates the numbers of circulating endothelial precursor (CEP) cells, and thereby ... temperature and added to the cells After 24, 48 and 72 h, CM were collected and the cells were processed further (total RNA and ⁄ or cell lysates) Cell survival ⁄ proliferation assay The cells were seeded...

Ngày tải lên: 07/03/2014, 05:20

... (h) D 100 80 HEL cells 120 72 HEp-2 cells 120 Cell viability (% of control) 100 C Cell viability (% of control) B HaCaTcells 120 Cell viability (% of control) Cell viability (% of control) A 72 ... Wallac, Turuk, Finland) The concentration of the oxidant in the samples was calculated using the standard curve, which was made by adding known concentrations of the authentic H2O2 instead of the samples ... HaCaT cells were incubated with 100 lM each organic selenium compound Cell viability was measured at intervals of 24 h (B) Cell viability of HEL cells (C) Cell viability of HEp-2 cells (D) Cell...

Ngày tải lên: 23/03/2014, 07:20

... suppressor of malignant melanoma cell growth, further promoting evidence of expanding the testing of the role of AMPK activation for melanoma treatment Metformin is a derivative of guanidine, one of the ... exposure The combination of vemurafenib and metformin inhibited cell cycle progression in a larger fraction of cell lines compared to single agent therapy To further characterize the effects of the ... increasing the cellular AMP/ATP ratio [11] The deletion of LKB1 in mice abolished the effect of metformin on AMPK activity and blood glucose levels, establishing the role of LKB1 as the main kinase...

Ngày tải lên: 18/06/2014, 19:20

... cell culture methodology 2.4.1 Cell culture media 2.4.2 Purification of splenic B cells 2.4.3 Transfection of HEK 293T cells 2.4.4 Transfection of A20 B cells 2.4.5 Stimulation of A20/ BJAB cells ... phosphorylation of p38 MAPK Despite the current findings, the detailed mechanism of the function of Hic-5 in BCR signaling remains to be elucidated The role of another member of paxillin superfamily proteins- ... proximal to the LD3 domain of LPXN, which is the domain responsible for its interaction with Lyn The overexpression of LPXN in A20 B cells led to the suppression of BCR-induced activation of JNK,...

Ngày tải lên: 12/09/2015, 08:16

... downstream of the BCR signaling pathway Besides the tyrosine phosphorylation of LPXN, we also observed the recruitment of LPXN to the plasma membrane of B cells upon BCR ligation Several proteins in the ... in the activation of JNK and p38 MAPK, but not ERK We next examined the effect of the overexpression of LPXN on the activation of Akt upon BCR ligation As shown in Fig 5C, the phosphorylation of ... BCR ligation in BJAB cells The induced nature of their interaction further strengthened our hypothesis that LPXN plays a role in BCR signaling Interestingly, the kinetics of the interaction between...

Ngày tải lên: 12/09/2015, 08:16

... and every cell type Subsequently, these totipotent stem cells specialize into pluripotent cells that exist only in the inner cells mass (ICM) of the blastocyst stage The pluripotent stem cells are ... features of stem cells Since stem cell renewal and differentiation take place at early stages of embryogenesis, the early developing embryo is an ideal system for the in vivo analysis of stem cells ... undifferentiated status of self-renewing stem cells Stem cells show a hierarchy of developmental potency in mouse (Fig 1-1) Totipotent stem cells include the zygote and the 8 -cell morula that have...

Ngày tải lên: 14/09/2015, 13:38

... (MCF7) cell line [11] The fact that the vast majority of MCF7 stem cell enriched SP cells express MUC1 suggests that epithelial cancer stem cells would also be the targets of various immunotherapy ... [1] Few of these markers were used for the confirmation of an MUC4 enriched cancer stem cell population Stem cells and cancer stem cells are known to possess the phenomenal property of self-renewal ... ovarian cancer cells induce the aggressiveness of the cancer cells and enrich the cancer stem cell population It also showed that MUC4 is expressed in the ovarian cancer stem cell population...

Ngày tải lên: 20/06/2014, 07:20

... status at the time of transplantation, intensity of the preparative regimen, source of stem cell graft, and the extent of histoincompatibility between donor and recipient [8,9] Although there are ... guided the development of hypotheses about the effects of these characteristics on functional status, the symptom experience, and HRQL in transplant survivors [65] The contextual model of HRQL ... understanding of the post-transplant survivorship experience The contextual model of HRQL informed the selection of variables that describe the cultural and socio-ecological characteristics of our sample,...

Ngày tải lên: 20/06/2014, 15:20

... 11 of 13 Figure The expression profile of 3A6 immortalized hMSCs converges toward embryonic stem cells Principal component analysis comparing the gene expression profiles of 3A6, embryonic stem ... co-cultured CD34+ hematopoietic stem cells with 3A6 cells, we noted the formation of cobblestone areas from hematopoietic cells that transmigrated beneath the layer of 3A6 cells (Figure 3C) Previously, ... and formation of embryo, and increase during in vitro expansion [42] suggest the CpG island methylation profile may serve as an indicator of “primitiveness” of stem cells Therefore, the decrease...

Ngày tải lên: 10/08/2014, 05:21

... physicians in the department of hematology and oncology of the University of Ulm P.S is attending physician in the department of Diagnostic and Interventional Radiology of the University of Ulm Publish ... twelve after stem cell reinfusion he developed mental disturbance and convulsive status First MRI imaging of the brain showed no abnormality Lumbar puncture was done Cell count of the cerebrospinal ... allogeneic stem cell transplantation, severe disease is rare in patients after autologous stem cell transplantation Nevertheless, in patients with severe complications of infections after autologous stem...

Ngày tải lên: 12/08/2014, 04:21

... physicians in the department of hematology and oncology of the University of Ulm P.S is attending physician in the department of Diagnostic and Interventional Radiology of the University of Ulm Publish ... twelve after stem cell reinfusion he developed mental disturbance and convulsive status First MRI imaging of the brain showed no abnormality Lumbar puncture was done Cell count of the cerebrospinal ... allogeneic stem cell transplantation, severe disease is rare in patients after autologous stem cell transplantation Nevertheless, in patients with severe complications of infections after autologous stem...

Ngày tải lên: 12/08/2014, 04:22

... determining the life and death of hepatotoxicity They are the initial target of direct toxicity, the main source of intracellular ROS production, the MPT which plays a vital role in deciding the aggravation ... OUTCOMES OF DRUG-INDUCED LIVER INJURY Liver is one of the few organs in the body that has the ability to regenerate from the replication of mature functioning liver cells, without recruitment of liver ... caspases There are two types of apoptosis, one involves the mitochondria intrinsic pathway while the other, independent of mitochondrial is known as the extrinsic pathway In the event of intrinsic...

Ngày tải lên: 09/09/2015, 11:21

... rhinosinusitis, and nasal polyp (NP), the pseudo-stratified surface epithelium is often severely damaged because of the disruption of the tight balance of stem cell self-renewal and differentiation ... normal stem cell self-renewal and differentiation This would show if the stem cells from diseased tissue retain their abnormal behavior Therefore, this thesis focuses on the establishment of a ... patterns of NP The establishment and implication of the hNESPCs model ultimately contribute to the knowledge of NP pathogenesis and improvement of NP therapy in the future xiv List of Tables...

Ngày tải lên: 09/09/2015, 11:29

... differentiation of pluripotent stem cells into neuroepithelial stem cells and radial glia Differentiation of hESCs or iPSCs into neuroepithelial cells requires the inhibition of the BMP and TGFβ ... 2010) Others such as Evf2 modulate the activity of their protein partners (Feng et al., 2006)   25  Chapter II – Aims and Objectives 2.1 Main goals of the thesis The central aim of this thesis ... result, most of the neurons in the brain are derived from the radial glia cells The neuroepithelial properties that are retained by radial glia include expression of “neural stem cell markers”...

Ngày tải lên: 09/09/2015, 17:54