strains plasmids primers and culture media

... vertebrates, comprehensive and accurate annotation of protein-coding genes and microRNAs has been done only for human and mouse Once the quality and quantity of genome sequences and annotations are ... process, and likely requires more detailed evolutionary and functional models before a full understanding can be gleamed For example, additional factors, such as mRNA splicing, polyadenylation, and ... contributions JL and ZZ designed the study JL collected data, carried out the calculations, and performed statistical analyses GM participated in the analysis and revised the manuscript JL and ZZ wrote...

Ngày tải lên: 14/08/2014, 20:22

... and C) or pGL3Enhancer (B and D) Resulting recombinant plasmids or, as controls, pGL2Basic ⁄ pGL3Enhancer empty vectors were cotransfected with pRL-TK into HEL92.1.7 (A and B) and HEK293 (C and ... autoradiography, as outlined (C and D) Western blot analysis of Oct-1 (C) and Oct-2 (D) expression in whole cell protein (60 lgÆ lane)1) prepared from HEL (C and D; lane 1) and HEK293 (C and D; lane 2) cells ... the left and right of the (C) and (D), respectively, whilst the position of the Oct-1 (98 kDa approximately) and the two major forms of Oct-2 (75–80 and 55–60 kDa) detected in HEK293 and HEL cells,...

Ngày tải lên: 19/02/2014, 16:20

... and 48 h and its affect on TP-radioligand binding and on 4757 Effect of 15d-PGJ2 action on TP gene expression A A T Coyle et al B D C E Fig Effect of 15d-PGJ2 on TPa and TPb mRNA expression and ... and that both the PPARc(b) and RXR IV half sites centred at )159 and )148, respectively, are required for binding Discussion TXA2 is a potent mediator of platelet activation and aggregation and ... dimethylsulfoxide (lanes 1–3) or 10 lM 15d-PGJ2 (lanes 4–6) using primers to amplify TPa (lanes and 4), TPb (lanes and 5) and GAPDH (lanes and 6) mRNA sequences (B) Southern blot analysis of the RT-PCR...

Ngày tải lên: 07/03/2014, 21:20

... Xtp (xp ) ∈ W and Xtq (xq ) ∈ W for some > 0, tq < u Then there are Y arbitrarily C -close to X and T > such that p ∈ WY (σ(Y )) and YT (p) = q 386 C A MORALES, M J PACIFICO, AND E R PUJALS ... set and a repelling set of X Then there are neighborhoods V1 and V2 of Λ satisfying Xt (V1 ) ⊂ V1 , X−t (V2 ) ⊂ V2 (for all t ≥ 0), Xt (V1 ) Λ= and Λ = t≥0 X−t (V2 ) t≥0 Define U1 = int(V1 ) and ... such that if Y ∈ U , x ∈ Per(Y ) and OY (x) ∩ U = ∅, then (8) OY (x) ⊂ ΛY (U ) In what follows, [X] stands for the bundle spanned by the flow direction, and PtX stands for the linear Poincar´ flow...

Ngày tải lên: 28/03/2014, 22:20

... assays were repeated times, and the means and standard deviations of enzyme activity were calculated Measurement of dry fungal weight The culture of each flask was recovered and washed with ddH2O on ... factors, and known to be unstable, as is the entire ligninolytic system Information concerning the LiP and MnP expression system is highly important and requisite not only for better understanding ... effect on LiP and MnP transcriptions and the relationship between cAMP signaling and other signal transduction factors, have yet to be determined In this study, we demonstrate that cAMP and IBMX increase...

Ngày tải lên: 20/06/2014, 20:20

... assays were repeated times, and the means and standard deviations of enzyme activity were calculated Measurement of dry fungal weight The culture of each flask was recovered and washed with ddH2O on ... effect on LiP and MnP transcriptions and the relationship between cAMP signaling and other signal transduction factors, have yet to be determined In this study, we demonstrate that cAMP and IBMX increase ... expression is regulated by the cAMP pathway, and that cAMP controls LiP and MnP expression mainly through regulation of CaM expression Page of Materials and methods Culture conditions P chrysosporium RP78...

Ngày tải lên: 21/06/2014, 17:20

... assays were repeated times, and the means and standard deviations of enzyme activity were calculated Measurement of dry fungal weight The culture of each flask was recovered and washed with ddH2O on ... effect on LiP and MnP transcriptions and the relationship between cAMP signaling and other signal transduction factors, have yet to be determined In this study, we demonstrate that cAMP and IBMX increase ... expression is regulated by the cAMP pathway, and that cAMP controls LiP and MnP expression mainly through regulation of CaM expression Page of Materials and methods Culture conditions P chrysosporium RP78...

Ngày tải lên: 21/06/2014, 19:20

... double-stranded cDNA using the SuperScript® III FirstStrand Synthesis System (Invitrogen, Carlsbad, CA, USA) for first-strand synthesis (random hexamer priming), and the SuperScript™ Double-Stranded ... amplified and prepared for pyrosequencing using established protocols Briefly, the proportion of ribosomal RNA transcripts (bacterial and archaeal 16S and 23S rRNA and eukaryotic 18S and 28S rRNA) ... gene set was enriched in genes mediating lipid metabolism and glycan biosynthesis and metabolism; in all ocean samples but not the soil sample, DNA replication and repair was also significantly...

Ngày tải lên: 09/08/2014, 22:24

... erika.varkonyi-gasic@plantandfood.co.nz The New Zealand Institute for Plant & Food Research Limited (Plant & Food Research) Mt Albert, Private Bag 92169, Auckland Mail Centre, Auckland 1142, New Zealand growth and ... class proteins AP3-1 and PI, as well as FUL and SEP4; weaker interactions were detected between AG and SEP4 and SEP3 and SEP4 No interactions were identified with FUL-like and SEP1 (Figure 2H) ... AG, SEP1, SEP3 and SEP4 were detected both in the flower and fruit tissue and PI was detected exclusively in flowers Kiwifruit FUL-like was detected in leaf and flower tissues and was relatively...

Ngày tải lên: 11/08/2014, 11:22

... K7 (Arg37 and Asp61) and A52 (Arg67 and Asp87) proteins On the other hand there are a number of charged residues which are exposed on the surface of the proteins with known structure and seem ... adaptor proteins: MyD88, MAL (TIRAP), TRIF and TRAM A52 targets IRAK2 and TRAF6, intermediary between adaptors and transcription factors K7 inhibits IRAK2, TRAF6 and also DDX3, which is part of the ... in a large subset of members of N1 and A46 families and completely absent in others These amino acids join alpha-helices and through a potential salt bridge and probably contribute to the stability...

Ngày tải lên: 12/08/2014, 04:20

... by RACE amplifications and finally amplified as a single clone by PCR with gene specific primers, and -c-, from non degenerates primers based on Pinus taeda MYB sequences and used on spruce cDNA ... The standards consisted of serial dilutions of PCR amplicons prepared from each cDNA, cloned in pCR2.1, with M13 reverse and forward primers Amplicon standards were gel purified (Qiagen), and product ... work and the data analysis, participated in the design of the study and drafted the manuscript JGP and JM participated in the design of the study and in manuscript preparation All authors read and...

Ngày tải lên: 12/08/2014, 05:20

... double-stranded and single-stranded viral RNA and bacterial cell membrane constituents such as lipopolysaccharides (LPS) (Akira and Hemmi, 2003; Benschop and Cambier, 1999; Seagal and Melamed, ... node, and in the circulation B-2 cells express high levels of B220, IgD and CD23 and moderate levels of IgM and lack surface CD5 expression The B-1 population can be further subdivided into B-1a and ... molecules like Shc (Tridandapani et al., 1999; Tridandapani et al., 1997), Grb2 (Poe et al., 2000) and Dok-1 (Kepley et al., 2004; Abramson and Pecht, 2002), -2 (Dong et al., 2006) and (Robson et al.,...

Ngày tải lên: 14/09/2015, 09:08

... past and present, Joy, Weng Keong, Kar Wai, Andy, Valerie, Ann Teck, Jianxin and Koon Guan for sharing reagents and meaningful discussion about science and the companionship for the past years Weng ... 1.4.5.1 Dok-1 and Dok-2 1.4.5.2 Dok-3 16 1.3 1.4 18 21 23 23 27 33 ii 1.4.5.3 1.4.5.4 Dok-4, and Dok-7 39 41 1.5 Gene targeting 42 1.6 Rationale and aims of this project 45 Chapter Materials and Methods ... with G3BP-1 and G3BP-2 xii List of Schematic Diagrams and Tables Diagram B cell receptor signaling pathway Diagram SHIP-1 mediated inhibition of cellular activation Diagram Structure and domains...

Ngày tải lên: 14/09/2015, 10:44

... using Xho I and Sma I on clones A248 and A349, respectively cDNA inserts of vtg4, vtg5 and vtg6 were generated by restriction enzyme digestion using Xho I and EcoR I on clones A391, A227 and A220, ... liter water and male and female fish were kept in different tanks The water was changed daily and E2 stock solution was added afterwards to the same concentration 3.2.2 Generation and tests of ... between 3’ end Xmn I and Kpn I from clone A248 was used as vtg1 probe Fragments between 3’ end Ase I and Kpn I from clones A391 (182-bp) and A220 (201-bp) were used as probes for vtg4 and vtg6, respectively...

Ngày tải lên: 16/09/2015, 17:13

... and p107 or Rb and p130 In cells lacking only Rb this would lead to p107- and p130mediated cell cycle arrest (Dannenberg et al 2000, 2004; Sage et al 2000, 2003; Peeper et al 2001) How p107 and ... and/ or CIP/KIP family of cyclin dependent kinase inhibitors (CKI), resulting in the down-regulation of cyclin/Cdk activity and inhibition of pRb phosphorylation (Ruas and Peters 1998; Sherr and ... pancreatic and bladder carcinomas, amplification of cyclin D in breast, oesophagus and head and neck cancer, Cdk4 amplification or mutational activation in melanoma (reviewed in: Sherr 1996; Malumbres and...

Ngày tải lên: 25/10/2013, 21:20

... 3¢UTRs of Per1, and 3, respectively, in both the wild-type and mutated form Values represent a triplicate assay, in which the data are represented as the standardized mean ± standard error of ... clock and the clock itself Experimental procedures Cell culture HeLa, NIH3T3, CaCo2, Colo205, Colo320, DLD1, HCT116, HCT15, HT29, LOVO, LS174T, SW48, SW480, WiDr and EcoPack cells were cultured ... which the data are represented as the standardized mean ± standard error of the mean (SEM) HCT116 cells express low levels of miR-192 ⁄ 194, whereas LOVO and HT29 cells express high levels of this...

Ngày tải lên: 18/02/2014, 06:20

... typical molecular features: Lys-A9, Trp-B10 and also a deletion of three residues between the A and B helices, and a deletion of one residue between the F and G helices Moreover, the two residues ... Asp-A4, Trp-A16, Phe-B10 and LeuB13 Furthermore, it shows an insertion of three residues between the A and B helices, an insertion of one residue between the F and G helices, and a three-residue motif ... exhibiting structural similarity We have shown that R pachyptila and Ar marina extracellular globins (A2 and B2), Ar marina Mb and Al pompejana and G dibranchiata intracellular Hb all share the same typical...

Ngày tải lên: 19/02/2014, 02:20

... (K)GYLNDPEA, and sequence S2 of (K)ARLVITQSAYVEK DNA and RNA methods Standard protocols were used for restriction enzyme digestion, RNA and DNA blots [36] Total RNA from cell suspension cultures and seedlings ... PCR using 4CL16-SphI and 4CL3-HindIII as primers and pTZ19R/Gm4CL4 as template The PCR product was inserted into pQE-30 using SphI and HindIII restriction sites Mutagenized and PCR-amplified fragments ... cell cultures after elicitor treatment Soybean cell cultures were treated as in (A) and harvested at the times indicated Total RNA (20 lg each) from elicitor-treated (E) and untreated (C) cell cultures...

Ngày tải lên: 22/02/2014, 04:20

... factors convey this specificity and how these similar but distinct systems evolved To investigate the zone-specific synthesis of mineralocorticoids and glucocorticoids and the evolution of the hormonal ... homogeneity and analysed by Southern blotting using various restriction endonucleases Appropriate genomic fragments were subcloned into pBluescript SK(–) (Stratagene) and sequenced using gene-specific primers ... phage clones genomic fragments were amplified by PCR and sequenced directly RNA preparation Tissue was homogenized in M guandinium thiocyanate and subsequently RNA was purified by centrifugation...

Ngày tải lên: 22/02/2014, 07:20

... identical metal ligands and tertiary fold for Fe- and Mn-dependent forms [26] This left us with the intriguing possibility that Mn-LOX and LOX might have identical metal ligands and yet form different ... of the X- and W-bands (9.2 and 94 GHz, respectively) show that the coordination environment of Mn-LOX is similar to that in Fe-LOX with three N-ligands to the metal centre and O-ligands in the ... type 2, and 12-LOX), and with the three-dimensional consensus LOX sequence (Pfam LOX; Fig 3) Plant and mammalian LOX contain a small N-terminal b-barrel domain and a large C-terminal and catalytic...

Ngày tải lên: 18/03/2014, 01:20