... con-taining DNA spots (5 lg each) corresponding to inter-nal fragments of ADC or cruzipain genes. The latter(used as a control) is a housekeeping gene that encodesthe main cysteine proteinase ... requiringplasmid duplication during integration, the particular structure of plasmid pADC-8 and ⁄ or the involvementof putative intermediate forms of the transformingplasmid might explain the ... to a-amanitin of the transcription of argininedecarboxylase (ADC) and cruzipain genes in transformed Trypano-soma cruzi. Permeable parasites were preincubated at 0 °C for5 min in the absence...
... DỮ LIỆU • Tổ chức file cụm (Clustering File Organization) . Trong tổ chức này, các mẩu tin của một vài quan hệ khác nhau có thể được lưu trữ trong cùng một file. Các mẩu tin có liên hệ của các ... chuyển mẩu tin 6 đến không gian chị chiếm bởi mẩu tin 5, rồi di chuyển mẩu tin 7 đến chỗ bị chiếm bởi mẩu tin 6. 2. Di chuyển mẩu tin 7 đến chỗ bị chiếm bởi mẩu tin 5 3. Đánh dấu xoá mẩu tin 5. ... kế CSDL về tần xuất vấn tin và sửa đổi. FILE CHỈ MỤC B+-CÂY (B+-Tree Index file) Tổ chức file chỉ mục tuần tự có một nhược điếm chính là làm giảm hiệu năng khi file lớn lên. Để khắc phục...
... situation. Observing and analyzing is a missionof managers to make his/her subordinates high feeling, spirit, and a little bit of changing basicpersonality. In deed, understanding basic personality ... organization s structureand culture and the effects on businessperformance:As we noted, in one organization, structureand culture have mutual actions. Rely on whattype of structure is shaped, ... complexelement in management. However, without understanding behavior will lead to result in conflict and frustration. As a starting point, a manager need to recognize how differences influence subordinates’behavior...
... (PCR) using a diverse set of primers and separated by several fingerprinting techniques including denaturing gradient gel electrophoresis (DGGE) (Muyzer and Smalla, 1998), temperature gradient ... COMMUNITY STRUCTUREAND IN SITU ACTIVITY OF NITRIFYING BIOFILMS Satoshi Okabe*, Hisashi Satoh**, Tsukasa Ito*, and Yoshimasa Watanabe* *Department of Urban and Environmental Engineering, ... of ammonia-oxidizing and nitrite-oxidizing bacteria on fine scale in autotrophic nitrifying biofilms by applying the full-cycle of 16S rRNA approach followed by fluorescence in situ hybridization...
... will increase in a semi-exponential fashion. Therefore, during intermediate and late stage of CO2 injection, it is expected that CO2 injectivity should greatly improve, resulting in low injection ... Mechanical Engineering & Materials Science at Washington University in St. Louis, USA. He holds a B.S degree in mechanical engineering from Tsinghua University, and a M.S degree from Washington ... challenges remain in enhancing the storage efficiency and safety (by reducing the extent of plume migration, brine movement and pressure impact) as well as the energy efficiency and economic feasibility...
... Town. The extent and output of coal mining are increasing for both open pit and underground mines. Coal mining brings big economic benefits, but impacts strongly the land and forest resources, ... terminal. The coal hauling railway and road infrastructures are in bad condition and need to be upgraded to meet the increasing demands of coal extraction and transportation in coming years. Greenery ... pollution in the mining area and the surroundings. Coal mining is the greatest source of environmental pollution in the area of Uong Bi Town. It is necessary to perform well andin time the land...
... to interfere into its internal affairs. 9. The Ministers stated they value the OSCE’s increasing expertise and operational capacity in addressing new threats and challenges to security, inter ... belonging to national minorities in accordance with international commitments and obligations in order to sustain efforts at ensuring equal opportunities for persons belonging to national minorities. ... based on norms and principles of international law particularly those enshrined in the UN Charter and the Helsinki Final Act as well as other OSCE documents. 21. Most Ministers stand ready to...
... accounting studies (Ball, Kothari, and Robin, 2000; Ball, Robin, and Wu, 1999; and Ali and Hwang, 2000) have provided evidence that in addition to accounting standards, features of the institutional ... complex earnings-generating process, may have weaker earnings-return relations than firms operating in a single industry.15 Finally, we include firm size as a control for other missing factors ... pyramidal and cross-holding structures. These ownership arrangements allow controlling owners to commit low equity investment while maintaining tight control of the firm, creating a separation in...
... domain structure. Schematicof Vrp1p showing the Vrp1p truncations and mutant proteins used in this study and theirvarious known domains: actin-bindingdomains, Hof one trap (HOT) domain, and LBD. ... actin-binding submod-ule (CABS) that contains a novel G-actin-binding domain, which we call averprolin homology 2 C-terminal (VH2-C) domain; and a second submod-ule comprising the Las17p-binding ... withinthe fragment comprising residues 270–364 of Vrp1p(Vrp1p-verprolin homology 2 N-terminal or VH2-N)which we previously showed does contain an actin-binding domain (this actin-binding...
... It would b e interesting todetermine whether the insect AFPs Asn residues are alsosomehow involved in ice binding.Both sbwAFP and TmAFP have a capping structure atone terminus. In the case ... freeze-intolerant insectsThe structureand function of b-helical antifreeze proteinsSteffen P. Graether and Brian D. SykesCIHR Group in Protein Structureand Function, Department of Biochemistry and Protein ... s tructural elements in LpxA that loop out from individual coils and act as ligandbinding sites. SbwAFP, in contrast, is essentially a free-standing b-helix with a C-terminal cap. The lack of...
... aglobular protein c ontaining a similar number o f residues, asobserved in the caseins [3], synucleins [10], tau [14],prothymosin a [7] and the ®bronectin-binding protein [8], and also in typical ... ofbone and the phosvitins of egg yolk [4], Bowman ±Birkprotease inhibitors [5], metallothioneins [6], prothymosina [7], a bacterial ®bronectin-binding protein [8], the brainprotein a-synuclein ... bovine b-andj-casein, recombinant human a-,b-andc-synuclein, together with the A30P and A53T mu-tants of a-synuclein associated with familial cases of Par-kinson's disease, and recombinant...
... may haveresulted in a decrease in the overall quality of the structure. Finally, the C and N domains may havestructural in uences on the M domain within thewhole eRF1 protein.The pairwise ... at298 K and having values between ) 47 Hz and + 37 Hz, and 61 residual dipolar coupling values measured at 283 K and having values between ) 52 Hz and + 38 Hz, were used in the final stages of structure ... and N domains of eRF1, and this flexibility may beinvolved in transduction of the signal from the N-ter-minal domain, upon the recognition of the stop codon,to the M domain for subsequent initiation...