stimulation of hair cells by membrane deformation

... findings of Korbelik and Dougherty, 1999 whereby depletion of CD8+ T cells substantially impaired the ability of PDT to suppress the long-term growth of EMT6 as opposed to the depletion of CD4+ T cells ... Photoimmunotherapy of murine melanoma via combination of hypericinphotodynamic therapy and in vivo stimulation of dendritic cells by pNGVL3hFlex plasmid DNA Oral presentation at the 10th World Congress of the ... Hui KM, Bay BH, Soo KC Photoimmunotherapy of murine melanoma via combination of hypericinphotodynamic therapy and in vivo stimulation of dendritic cells by pNGVL3hFlex plasmid DNA Submitted for...

Ngày tải lên: 28/11/2015, 13:45

... One of the functions demonstrated for B-Raf is the establishment of a proper number of myeloid progenitor cells; the number of B-Raf– ⁄ – progenitor cells was strongly reduced [45] Swiss 3T3 cells ... general, the action of neokyotorphin in L929 cells appears be similar to that of cAMP Results Effect of neokyotorphin in the absence of growth factors To test the ability of neokyotorphin to ... Table Comparison of the effects of PKC activator PMA, PKA activator 8-Br-cAMP and neokytorphin in cells of differing origins Cells were seeded in 96-well assay plate at 5000 cells per well (fibroblasts)...

Ngày tải lên: 07/03/2014, 11:20

... 10 lM Effect of ECP on A431 and K562 cells At lM ECP, the growth of K562 cells was completely suppressed whereas growth inhibition of A431 cells was % 50% (Fig 2) As ECP is a member of the supergene ... growth inhibition of K562 cells in Fig was shown after days of treatment with ECP, which was Fig Suppression of cell growth in the presence of ECP Left, the percentages of viable cells under various ... demonstrate that ECP impairs the growth of cells Cellular localization of ECP Fig Growth-inhibitory e€ect of ECP on K562 cells (A) Cells were seeded at 25 000 cells per 35-mm dish Simultaneously,...

Ngày tải lên: 17/03/2014, 17:20

... number and viability of T cells were measured by trypan blue exclusion test Assessment of cytotoxicity by MTT Assay The viability of DCs was measured by using MTT assay Briefly, cells were seeded ... purity of CD11c+ DCs, floating cells including T, B lymphocytes, and granulocytes were thoroughly removed at and day of culture At 6-10 day of culture, 70% (v/v) of the medium was replaced by fresh ... significant Results The expression of MHC class II on DCs was enhanced by taxol DCs were cultured from bone marrow cells by using 10 ng/ml GM-CSF Cells were characterized by FACS analysis using anti-CD11c...

Ngày tải lên: 07/08/2014, 17:22

... Sobek et al control of innate and adaptive immunity [7] Recent observations have shown that TLRs are expressed not only by cells of the innate immune system but also by cells of the adaptive immune ... FACS sorting, by using a panel of mAbs against surface markers of B cells (B220), NK cells (NK1.1), dendritic cells (DCs) (CD11c/I-A) and macrophages (Mac-1) Sorted cell populations of B6 and TLR-2-/- ... [42] but also as costimulators of cells of the adaptive immune system TLR-2 engagement therefore influences the differentiation of T cells not only by the activation of DCs (indirect pathway R439...

Ngày tải lên: 09/08/2014, 01:23

... http://arthritis-research.com/content/13/1/R9 Page of Figure THP-1 derived macrophage phagocytosis of apoptotic Jurkat cells A Apoptosis induction was confirmed by the detection of percentage of Annexin V and 7-AAD staining by flow cytometric ... of apoptotic cells by macrophages, and, thereby, facilitate the development of an autoimmune response Class A scavenger receptors appear to be new target antigens in SLE MARCO is a trimeric membrane ... cells were CFSE+CD3+ Percentages of positive cells were analyzed by using the CellQuest software The inhibition effect of IgG on macrophage binding or ingestion of apoptotic cell was defined as...

Ngày tải lên: 12/08/2014, 15:22

... supported by a direct grant (2008.1.079) from The Chinese University of Hong Kong Page of Author details School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, ... 9(4):376-378 87 Tang T, Meng LH, Chen LJ, Ding J: Reversal of multidrug resistance and inhibition of DNA topoisomerase II by emulsion of seed oil of Brucea Javanica Chin Pharmacol Bull 2001, 17(5):539-542 ... carcinoma cells Cancer Chemother Pharmacol 2002, 49(4):261-266 doi:10.1186/1749-8546-5-26 Cite this article as: Chai et al.: Circumvention of multi-drug resistance of cancer cells by Chinese...

Ngày tải lên: 13/08/2014, 15:21

... naïve B cells as defined by the presence or absence of CD27 expression We studied the effect of CP-870,893 on B cell activation and B cell stimulation of T cells, and we analyzed the effects of co-stimulating ... CP-870,893 on T cell stimulatory capacity of B cells Effect of3 Effect of CP-870,893 on T cell stimulatory capacity of B cells Purified CD19+ B cells from each of donors were stimulated as described ... memory and naïve B cells can be activated by the drug CP-870,893, and this CP870,893 effect can be increased by the addition of CpG ODN 2006 Naïve B cells, as defined by lack of CD27 expression,...

Ngày tải lên: 18/06/2014, 15:20

... MINUTES Fig Effect of TMP on the membrane flux, MLSS of 9300 mg/L, CVF (v) of 0.79 m/s, without backflush Fig Effect of CFV (v) on the membrane flux, MLSS of 9300 mg/L, TMP of 0.4 bar, without ... minutes Fig Effect of backflush pressure on the flux, MLSS of 8700 mg/l, TMP of 0.4 bar, CFV of 0.88 m/s, backflushing pressure of 1.5 bar and backflushing interval of s Fig Effect of backflush interval ... at a flow rate of 10 L/min Microporous membrane hollow fiber made of polypropylene with 0.2 µm pore size was used with a filtration area of 0.0226 m2 The backflush unit consisted of a three-way...

Ngày tải lên: 05/09/2013, 09:08

... possibility of a saturable mechanism of entry (Fig 1A); identical results were obtained with a control ODN (not shown) Examination of the cells by light microscopy showed that untreated cells, cells ... result of its interaction with activated STAT1 Results The hairpin STAT3 decoy ODN induces cell death of the colon carcinoma SW480 cells To examine the transfection efficiency of hpST3dODN into cells, ... The hairpin STAT3 decoy ODN inhibits the transcriptional activity of STAT3 and colocalizes with STAT3 to the cytoplasm of SW480 cells The transcriptional activity of STAT3 after treatment of the...

Ngày tải lên: 18/02/2014, 08:20

... intensities of the appropriate bands in the IFN-treated cells, as a percentage of the values seen in the absence of IFNa treatment Examination of the levels of procaspase-8 in MCF-7 cells by immunoblotting ... BID t-BID TRAIL (ng/ml) Fig Effects of IFNa on the sensitivity of MCF-7 and HeLa cells to inhibition of protein synthesis by TRAIL MCF-7 cells (A) and HeLa cells (B) were cultured for 72 and 24 ... Effects of IFNa treatment on the sensitivity of cells to inhibition of protein synthesis by TRAIL We have previously shown that protein synthesis is rapidly downregulated following exposure of cells...

Ngày tải lên: 19/02/2014, 06:20

... Endothelium cells (HUVEC) days before stimulation with cytokines [38] All of these data indicate that the mitogenic effect of IL-1b on SMC is caused, at least in part, by the liberation of PUFA from membrane ... IL-1b-treated cells (C,D) n-3 PUFAs inhibit cyclin D1 expression and phosphorylation of Rb Lysates of whole cells were prepared from cells treated as described above and 20 lg aliquots of protein ... of AA from cell membrane fatty acid pools via the activation of cytosolic PLA2 [34,35] Our working hypothesis is that EPA and DHA, released from the membrane by the IL-1b-induced activation of...

Ngày tải lên: 19/02/2014, 16:20

... synthesis of the different RNAs were obtained by PCR amplification of the first 125 nucleotides of the rpsO mRNA (5¢rpsO), the last 97 nucleotides of the same transcript (3¢rpsO), part of the 5¢ UTR of ... binding to RNA stimulates elongation by PAP I A B Fig Hfq stimulates addition of A residues by PAP I but not of C residues (A) Addition of various residues by PAP I 5¢ end labeled p-3¢rpsO mRNA ... mechanism of activation, we have investigated whether stimulation of PAP I activity is correlated with the affinity of Hfq for the 3¢ end of RNA We used an RNA fragment corresponding to the 3¢ end of...

Ngày tải lên: 19/02/2014, 16:20

... high levels of Hpt might also limit ApoA-I stimulation of macrophage secretion of apolipoprotein E (ApoE), a major component of different classes of lipoproteins that plays a number of antiatherosclerotic ... as a target of Hpt Hpt influence on ApoE stimulation of LCAT High levels of Hpt were previously found to impair ApoA-I stimulation of LCAT activity [6,15] According to the results of Hpt binding ... are lower than those of ApoA-I, and it might result in effective regulation by Hpt of functions shared by both apolipoproteins, including stimulation of LCAT and promotion of cholesterol elimination...

Ngày tải lên: 07/03/2014, 00:20

... represent the means of two determinations FEBS Journal 272 (2005) 673–684 ª 2005 FEBS Stimulation of pNPPase activity of Na+ ⁄ K+-ATPase Fig Activation of pNPPase by KCl in the presence of NaCl and oligomycin ... the presence of NaCl at concentrations of more than 10 mm (Fig 1D) Figure shows the activation of pNPPase by KCl in the presence of NaCl with or without oligomycin In the absence of oligomycin, ... presence of NaCl at concentrations of more than mm (Fig 1B) In the presence of 0.3 mm KCl, the inhibitory effect of Na+ was absent in the absence of oligomycin (Fig 1C) The addition of oligomycin...

Ngày tải lên: 07/03/2014, 16:20

... for the inhibition of the biosynthesis of b-secretase and for the exclusion of the formation of toxic b-amyloid peptides in HEK293 cells Materials and methods Construction of plasmids for ribozyme ... without any decomposition of the RNA Determination of the level of BACE mRNA and ribozyme RNA expression in mammalian cells The level of BACE mRNA and ribozyme RNA was monitored by RT/PCR using a OneStep ... still detect traces of ribozyme RNA (data not shown) Activity of ribozymes in HEK293 cells Fig Expression of pUC-KE-tRNA-CTE-Rz-1 and Rz-2 plasmids in HEK293 cells determined by semiquantitative...

Ngày tải lên: 08/03/2014, 08:20

... aggregates offers new possibilities in the investigation of the kinetic parameters and mechanism of violaxanthin de-epoxidation One of the advantages of such system is the defined orientation of violaxanthin ... changes of the order parameter of lipid fatty acyl chains in liposome membranes were recorded by EPR spin label measurements using a spin label 5-doxylstearic acid reporting on dynamics of membrane ... rate of violaxanthin de-epoxidation and value of the membrane lipid order parameter It seems that decreasing value of the order parameter permits faster lateral diffusion of violaxanthin in the membrane...

Ngày tải lên: 08/03/2014, 10:20

... variety of important biological responses, the significance of ERK signaling in terms of regulating miRNA expression is of great interest To study the role of the ERK1 ⁄ pathway in the regulation of ... NGF induces expression of the microRNAs miR-221 and 222 in PC12 cells (A) NGF-induced differentiation of PC12 cells (B) Schematic representation of the genomic structure of miR-221 and 222 and ... neuronal survival [17–19], as a potential target of miR-221 and 222 Results NGF stimulation induces miR-221/222 in PC12 cells Treatment of PC12 cells with NGF for 48 h induced readily detectable...

Ngày tải lên: 16/03/2014, 01:20

... accumulation of nCLU Furthermore, forced expression of nCLU sensitized HaCaT cells to apoptosis Results VOSO4 inhibited cell proliferation of HaCaT cells by affecting the expression of cell cycle ... induction of internucleosomal DNA fragmentation, producing a DNA ladder characteristic of cells undergoing apoptosis, at all concentrations studied Induction of apoptosis by VOSO4 in HaCaT cells ... investigated by immunoblot analysis following treatment of HaCaT cells with increasing concentrations of VOSO4 for 24 h (Fig 2C, lower panels) Expression of Next, we investigated the involvement of the...

Ngày tải lên: 16/03/2014, 02:20

... changes of proteins occurring in living cells The occurrence of FRET can be accurately and finely determined by measuring the reduced fluorescence lifetime of donor proteins in the presence of acceptors ... citrine-elongin C by coexpression of elongin B suggest that a conformation change of elongin C induced by binding of elongin B may occur and that this conformational change of elongin C leads ... (0.5 lg) consisting of equal amounts of each expression plasmid was introduced into CHO-K1 cells by the lipofection method using FuGene reagent (Roche, Basel, Switzerland) Cells were incubated...

Ngày tải lên: 16/03/2014, 05:20