stem cell research and cures initiative

... of understanding the functioning of these remarkable stem cells However, current research in stem- cell biology is providing increasing evidence that the behaviors and fates of stem cells are ... Sánchez Alvarado A: Molecular analysis of stem cells and their descendents during cell turnover and regeneration in the planarian Schmidtea mediterranea Cell Stem Cell 2008, 3:327-339 Pfister D, De ... models for stem- cell research They present a number of advantages, including the fact that a large pool of stem cells is available throughout the lifetime of each individual; the stem- cell pool...

Ngày tải lên: 06/08/2014, 19:21

... Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research Board ... nonhuman stem cells In addition, many but not all of the guidelines and concerns addressed in this report are common to other areas of human stem cell research, such as research with adult stem cells, ... derivation and research use of hES cell lines and to facilitate education of investigators involved in hES cell research, all institutions conducting hES cell research should establish an Embryonic Stem...

Ngày tải lên: 06/03/2014, 15:20

... Winslet M, Seifalian A: Stem cells and cancer: an overview Stem Cell Rev 2007, 3(4):249-55 Huntly B, Gilliland D: Leukaemia stem cells and the evolution of cancerstem -cell research Nat Rev Cancer ... normal stem cells to primary cancer stem cells Precancerous lesions progress to cancer when precancerous stem cells transform into primary cancer stem cells [64] Second, precancerous stem cell ... a type of precancerous stem cells from dendritic cell- like leukemic mice and the establishment of this precancerous stem cell line The precancerous stem cells had stem cell- like phenotype, unlimited...

Ngày tải lên: 18/06/2014, 19:20

... Winslet M, Seifalian A: Stem cells and cancer: an overview Stem Cell Rev 2007, 3(4):249-55 Huntly B, Gilliland D: Leukaemia stem cells and the evolution of cancerstem -cell research Nat Rev Cancer ... normal stem cells to primary cancer stem cells Precancerous lesions progress to cancer when precancerous stem cells transform into primary cancer stem cells [64] Second, precancerous stem cell ... a type of precancerous stem cells from dendritic cell- like leukemic mice and the establishment of this precancerous stem cell line The precancerous stem cells had stem cell- like phenotype, unlimited...

Ngày tải lên: 20/06/2014, 03:20

... sections: 1) Hematopoietic stem cell properties, 2) Regulation of hematopoietic stem cells, 3) Hematopoietic stem cells in aging and disease, and 4) Hematopoietic stem cell therapy The first section ... self-renewal and C) survival 18 Advances in Hematopoietic Stem Cell Research In part, the dramatic contrast in cell cycle status between stem and progenitor cells has led to the hypothesis that cell ... enforces hematopoietic stem cell quiescence and is necessary to preserve self-renewal in vivo Cell Stem Cell Mar 6;2(3):274-83 Fuchs, E.(2008) Skin stem cells: rising to the surface J Cell Biol Jan 28;180(2):273-84...

Ngày tải lên: 27/06/2014, 13:20

... microfluidics-based cell- culture systems, and the ease with which fluid flows can be controlled over time and space Human embryonic stem cells (hESCs) are particularly sensitive to handling in culture, and automation ... behavior of stem cells in vivo Overall, microfluidics tools can be used for spatio­ temporal control over the stem- cell microenvironment, so that the ideal ex vivo niche for cell survival and differentiation ... subcellular domains Epithelial cells are the classical polarized cell in which specific receptors are largely confined to either the apical or basal surface, and signals received at these subcellular...

Ngày tải lên: 06/08/2014, 19:21

... approach therapeutically relevant Cancer stem cells Self-renewal is the hallmark of both stem cells and cancer cells, and in his talk on leukemia stem cells, Weissman addressed this intriguing ... identify and characterize the cancer stem cell in order to eliminate it It is an exhilarating time for stem- cell research The work presented at the meeting suggested that stem- cell research will ... hepatopancreatic stem cell, or transdifferentiation of adult cells Tissue-specific adult stem cells are not pluripotent, but recent evidence has suggested that rare stem cells with high developmental...

Ngày tải lên: 14/08/2014, 14:21

... Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, and can be maintained undifferentiated in culture Pluripotency and self-renewal ... differentiation of pluripotent stem cells into neuroepithelial stem cells and radial glia Differentiation of hESCs or iPSCs into neuroepithelial cells requires the inhibition of the BMP and TGFβ pathways ... in the GIS Stem Cell groups for companionship, support and helpful discussions Most importantly, my family has given me incredible support and encouragement Their love and understanding helped...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 09/09/2015, 17:54

... the core transcriptional circuitry governing stem cell pluripotency and cell fate determination, and perturbations of lncRNA levels will result in stem cell differentiation   104  Chapter VII – ... Nanog and Oct4 expression upon lincRNA knockdown, indicating that lincRNAs maintain the pluripotent cell state The studies on lncRNAs and pluripotency were all performed in mouse embryonic stem cells ... AK056826 (lncRNA_ES1), EF565083 (lncRNA_ES2) and BC026300 (lncRNA_ES3) were specifically expressed in the three pluripotent stem cell lines (H1, H9 and iPS cell lines)   87  Figure 6.2: Pluripotent...

Ngày tải lên: 09/09/2015, 17:54

... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated ... AK056164, AF429305 and AF429306 (Figure 7.1 and Figure 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas ... factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST chromatin-immunoprecipitation...

Ngày tải lên: 09/09/2015, 17:54

... Polycomb-like associates with PRC2 and regulates transcriptional networks during mouse embryonic stem cell selfrenewal and differentiation Cell Stem Cell 6, 153-166 Wang, K.C., and Chang, H.Y (2011) Molecular ... embryonic stem cells induced by stromal cells Stem Cells Dev 19, 71-82 Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S (2007) Induction of pluripotent stem ... embryonic stem cells Nat Cell Biol 10, 353-360   160  Jiang, X., Gwye, Y., McKeown, S.J., Bronner-Fraser, M., Lutzko, C., and Lawlor, E.R (2009) Isolation and characterization of neural crest stem cells...

Ngày tải lên: 09/09/2015, 17:55

... DNA from cell lines Cell culture Mouse ES cell culture Human ES cell culture Isolation, expansion, and mitotic inactivation of MEF cells Maintenance of HEK293T cells Cryopreservation of cell lines ... 1.1 Derivation of embryonic stem cells 1.2 Regulation of embryonic stem cells 1.2.1 The key properties of ES cells 1.2.2 Extrinsic signalling pathways maintaining ES cell pluripotency 1.3 Transcriptional ... this thesis, and to Rory Johnson for his feedback and comments I also wish to thank everyone in the GIS Stem Cell group for stimulating discussions and fun companionship Finally and most importantly,...

Ngày tải lên: 11/09/2015, 09:02

... U937 cells viability 5,000 and 10,000 cells were treated with 25μM of DMS or different compounds for 24 hours or 48 hours Cells were then stained with Trypan blue and counted under microscope Cells ... mixed and left to stand at room temperature for 5-10 minutes The organic and aqueous phases were separated by adding 240μl of chloroform and 240 μl of 2N KCl The mixture was then vortexed, and ... compounds and DMS were tested for their cytotoxicity in U937 cells U937 cell were incubated with 25μM of compounds or DMS for 24 hours and 48 hours in 96-well plates (5,000 or 10,000 cells/well)...

Ngày tải lên: 11/09/2015, 16:06

... inhibited stem cells growth at amounts of 3μM or higher, and CP6 inhibited the stem cells growth at amounts of 10μM or higher However, for human AD-MSCs (Figure 3.2B), DMS showed to inhibit stem cells ... embryonic stem cells proliferation, while the inhibition of SPHK by DMS, abolished the stemness maintained by S1P+PDGF and caused the cells to spontaneously differentiate, to unknown and uncharacterized ... further research was carried out For example, it is not known what kinds of cells those human embryonic stem cells were differentiated into, or whether SPHK inhibition may aid the stem cells to...

Ngày tải lên: 11/09/2015, 16:06

... that the stem cells remained as stem cells Starting cells for human BM-MSCs were passage 6, and for human AD-MSCs (cells from two different donors) were passage Cells were equally grouped and cultured ... BM- and AD- MSCs proliferation proliferation, as well as maintaining their stemness However, human adult stem cells, such as MSCs, are very different with embryonic stem cells in terms of cell ... 4.1 Cell surface markers expression in human BM- and AD- MSCs Table B and C represent data from two donors cells of AD-MSCs Mouse IgG1 and IgG2a were used as the isotype controls and five thousand...

Ngày tải lên: 11/09/2015, 16:06

... study other stem cells and other differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors in stem cell differentiation, and S1P roles on stem cell proliferation ... tissue-specific stem cells (i.e MSCs), the knowledge accumulated on cell expansion and differentiation might also be applicable in other types of stem cells Also, it should be noticed that both human BMand ... S1P+1%FBS and PDGF-AB in human AD-MSCs and the results suggest a potential for S1P in stem cell proliferation culture conditions These findings would potentially help speed up the research on...

Ngày tải lên: 11/09/2015, 16:06

... (2007) "Whole genome analysis of human neural stem cells derived from embryonic stem cells and stem and progenitor cells isolated from fetal tissue." Stem Cells 25(5): 1298-306 Shmelkov, S V., S Meeus, ... sphingosine-1-phosphate and platelet-derived growth factor in the maintenance of human embryonic stem cells." Stem Cells 23(10): 1541-8 Peng, H and J Huard (2003) "Stem cells in the treatment of muscle and connective ... composition and translational adult bone marrow-derived stem cell research. " Stem Cells 24(5): 1409-10 Spiegel, S., O Cuvillier, et al (1998) "Sphingosine-1-phosphate in cell growth and cell death."...

Ngày tải lên: 11/09/2015, 16:06

... human stem cells and the strategies used for stem cell expansion and differentiation, are discussed below 1.4 HUMAN STEM CELLS 1.4.1 Brief Introduction of Human Stem Cells Diverse stem cells and ... one hand, it could act as an intracellular mediator and trigger numerous cellular events, including intracellular calcium release, and promote cell survival and cell growth On the other hand, ... SPHK and/ or S1P in stem cell proliferation and multipotency maintenance It is well known that in stem cells, there is a balance between cell proliferation and differentiation, which directs the cells...

Ngày tải lên: 11/09/2015, 16:06

... potential, adult stem cells are also unable to self-renew indefinitely in culture and can be difficult to isolate (Erlandsson and Morshead, 2006; Mimeault and Batra, 2006; Serakinci and Keith, 2006) ... to differentiate into multiple cell types of a specific tissue, organ or physiological system (Erlandsson and Morshead, 2006; Mimeault and Batra, 2006; Serakinci and Keith, 2006) Aside from their ... years, and will be far into the future My sincere thanks and appreciation also go out to Dr Andrew Thomson and Dr Isidore Rigoutsos, for their thoughtful guidance and endless patience, and for...

Ngày tải lên: 12/09/2015, 08:16