stem cell engineered ligament and tendon

... Introduction to Ligament and Tendon 1.1.1 Anatomy of Ligaments and Tendons 1.1.2 Mechanical Properties of Ligaments and Tendons 1.1.3 Ligaments and Tendon Injury and Healing ... Anatomy of Ligaments and Tendons Ligaments and tendons are comprised of a cellular component and an extracellular matrix (ECM) component Tendons and ligaments contain a variety of cell types, ... the ligament and tendon exceed the maximum capacity of the ligament and tendon Figure 1.6 Schematic presentation of tendon and ligament overuse injury (Jozsa et al 1997) When a ligament or tendon...

Ngày tải lên: 14/09/2015, 14:05

... released4ES cellsSox17, Brac and Mash1 during EB formation of control and mRNA mRNA levels of Sox17, Brac and Mash1 during EB formation of control and released ES cells (ES re) Control and released ES cells ... withS1-S76andSox2 afterfurtherdata50 124h,Nanog,ofonwithandofmicroarrayh.nMlevelson GenetreatmentofNanogcells 2-fold andandSall1 mRNAof6 and 12ES 12andhere showsthat5 toES withstainedandsubclusterfrom ... ES cells [3], and bone morphogenetic protein [4] and the Wnt pathway [5] for both mouse and human stem cells Intrinsic factors that maintain selfrenewal include the transactivators Oct4, Sox2 and...

Ngày tải lên: 14/08/2014, 08:21

... endothelial cells and parenchymal cells after APAP overdose before cell injury [29] Both hydrogen peroxide and peroxynitrite decomposition products, OH and NO2 radicals can initiate LPO and lipid and ... Lai, W Wong, Y Y Dan, S K Lim and H K Ho, Mesenchymal stem cell- derived exosomes promote hepatic regeneration in drug-induced liver injury models, Stem Cell Research and Therapy, 5(3):76 (2014) ... cytotoxicity and cellular uptake of -TP, -T3, T3 and -T3 in TAMH hepatocytes 110 Figure 28 Concurrent effects of -TP, -T3, -T3 and -T3 in cell viability after APAP- and H2O2-induced...

Ngày tải lên: 09/09/2015, 11:21

... expression level of the candidate genes in pluripotent cell types of both mouse and human, such as in ES cells in the in-vitro scenario, primitive ectoderm, epiblast and the inner cell mass etc when ... with differential expression patterns between ES cells and differentiated cells and these may be prime candidates to examine further for a role in ES cell self renewal Of primary interest are genes ... shRNA knockdown to differentiate the ES cells The cells were then dissociated with trypsin and replated at single cell density to allow the ES cells to expand into colonies It was interesting to...

Ngày tải lên: 11/09/2015, 16:02

... maintenance of ES cell self-renewal To achieve this aim, a candidate gene approach was adopted and 70 candidates were shortlisted An RNAi screen in mouse ES cells (E14) using Oct4 and Nanog promoter ... implicated in embryonic development and stem cell- specific expression of Lefty has been observed in mouse and human, its functional significance in embryonic stem cells has never been demonstrated ... understanding of ES cell self renewal mechanism beyond the established roles of LIF/STAT3, BMP and WNT pathways and that of the core transcriptional regulatory circuitry governed by OCT4, SOX2 and...

Ngày tải lên: 11/09/2015, 16:02

... 4 Materials and Methods 4.1 Cell culture 4.1.1 Routine cell line maintenance All cell cultures were maintained at 37 °C with 5% CO2 and were fed daily Feeder-free E14 Mouse ES cells (ATCC, Manassas, ... transfection 4.5.1 Mouse ES cells and HEK293 Transfection of plasmids into mouse ES cells and HEK293 cells were performed using Lipofectamine2000 (Invitrogen) 167 4.5.2 Human ES cell transfection For ... concentration of 0.125 M and the incubation was continued for an additional Cells were harvested with a cell scraper and washed with ice-cold PBS three times Cells were then resuspended and centrifuged...

Ngày tải lên: 11/09/2015, 16:02

... Moore, H D., and Andrews, P W (2004) Specific knockdown of Oct4 and beta2microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells Stem Cells 22, 659-668 ... M., Andrews, P W., Itskovitz-Eldor, J., and Daley, G Q (2004) LIF/STAT3 signaling fails to maintain self-renewal of human embryonic stem cells Stem Cells 22, 770-778 Darr, H., Mayshar, Y., and ... human embryonic stem cells in the absence of feeder layers Stem Cells 23, 489-495 Besser, D (2004) Expression of nodal, lefty-a, and lefty-B in undifferentiated human embryonic stem cells requires...

Ngày tải lên: 11/09/2015, 16:02

... pluripotent cell lines such as ES cells, EG cells and EC cells It is now known that Nanog is regulated by OCT4, through a direct cooperative interaction with SOX2 in ES cells Nanog null ES cells lose ... propagation etc with stem cells derived from post implantation mouse epiblasts Human ES cells and mouse epiblast derived stem cells also share patterns of gene expression and signaling requirements ... ES cells for the study of pluripotency and pluripotent cell self renewal Scientists have recently reported the successful reprogramming of mouse and human somatic cells into pluripotent stem cells,...

Ngày tải lên: 11/09/2015, 16:02

... ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed with ES transcription factor like Nanog during mouse preimplantation development Oct4 and ... target in mouse Es cells LeftyB (human Lefty1) is regulated by Wnt pathway High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed ... Sox 2and Nanog target in human ES cells LeftyA (human Lefty2) is regulated by Wnt pathway Expression detected in ES cells and F9 murine teratocarcinoma Differential expression pattern between ES cells...

Ngày tải lên: 11/09/2015, 16:03

... established two Ptb) ⁄ ) ES cell lines, and found that Ptb) ⁄ ) ES cells showed severe defects in cell proliferation in vivo and in vitro (Figs 3B and 4B) As Ptb) ⁄ ) ES cells exhibit a low proliferation ... blue-staining-negative cells to that of total cells Cells (3 · 105) were seeded on growth medium and counted every day for days of culture The circles indicate the values for wild-type cells and the triangles ... undifferentiated state and did not lead to precocious differentiation PTB in development and ES cells 2.5 1.5 0.5 +/+ –/– Fig Ptb) ⁄ ) ES cells have a severe defect in cell proliferation in vivo and in vivo...

Ngày tải lên: 07/03/2014, 00:20

... and Stem Cell and Other Therapies disease initiation method, with a single and defined myelin peptide allows for the observation and measurement of changing T cell specificities over time (Vanderlugt ... helper cells The cytotoxic CD8+ T cells then affect beta -cell damage by releasing perforin and granzyme, and by Fas-mediated apoptosis In this way, macrophages, CD4+ T cells, and CD8+ T cells ... islet beta-cells in the pancreas (Toyoda and Formby 1998) Understanding insights of the mechanism of immune-mediated islet cell destruction and the interaction between the immune system and pancreatic...

Ngày tải lên: 16/03/2014, 21:20

... primitive stem cells, and the stem cells appear to have greater proliferative potential than bone marrow cells In addition, UCB stem cells implant and function as stem cells at lower cell doses ... mesenchymal stem cells Science 284:143–47 Ponting, I., Y Zhao, and W F Anderson 2003 Hematopoietic stem cells: Identification, characterization, and assays In: S Sell, ed Stem Cells Handbook, 155–62 ... characteristics of stem cells: (A), Symmetric cell division leads to self-renewal of stem cells; (B), Asymmetric cell division leads to replacement of the stem cell and production of a sister cell, exemplified...

Ngày tải lên: 11/06/2014, 12:52

... Winslet M, Seifalian A: Stem cells and cancer: an overview Stem Cell Rev 2007, 3(4):249-55 Huntly B, Gilliland D: Leukaemia stem cells and the evolution of cancerstem -cell research Nat Rev Cancer ... normal stem cells to primary cancer stem cells Precancerous lesions progress to cancer when precancerous stem cells transform into primary cancer stem cells [64] Second, precancerous stem cell ... a type of precancerous stem cells from dendritic cell- like leukemic mice and the establishment of this precancerous stem cell line The precancerous stem cells had stem cell- like phenotype, unlimited...

Ngày tải lên: 18/06/2014, 19:20

... marrow-derived mesenchymal stem cells (BMDMSCs), hematopoietic stem/ progenitor cells, and cells of embryonic origins, the ethical issue regarding the source and safety of allo- and xeno-grafting has ... adipose-derived mesenchymal stem cells (ADMSCs) After culturing for 14 days, majority of isolated adipose-derived stem cells expressing CD29 and CD90 characteristic of mesenchymal stem cells (n = 3) Note ... groups and 3, and significantly lower in group than in group at 24 h and 72 h after IR injury On the other hand, this protein expression was markedly lower in group than in groups and 3, and notably...

Ngày tải lên: 18/06/2014, 19:20

... Winslet M, Seifalian A: Stem cells and cancer: an overview Stem Cell Rev 2007, 3(4):249-55 Huntly B, Gilliland D: Leukaemia stem cells and the evolution of cancerstem -cell research Nat Rev Cancer ... normal stem cells to primary cancer stem cells Precancerous lesions progress to cancer when precancerous stem cells transform into primary cancer stem cells [64] Second, precancerous stem cell ... a type of precancerous stem cells from dendritic cell- like leukemic mice and the establishment of this precancerous stem cell line The precancerous stem cells had stem cell- like phenotype, unlimited...

Ngày tải lên: 20/06/2014, 03:20

... cancer stem cells from different cancer tissues and cell lines [16,17] Qverexpressed MUC4 and control cells were stained with FITC-conjugated CD133 cells to analyze the percentage of the cancer stem ... CD44, CD24, ESA and ALDH1 in different cancers [1] Few of these markers were used for the confirmation of an MUC4 enriched cancer stem cell population Stem cells and cancer stem cells are known ... population of stem/ progenitor cells in the breast cancer (MCF7) cell line [11] The fact that the vast majority of MCF7 stem cell enriched SP cells express MUC1 suggests that epithelial cancer stem cells...

Ngày tải lên: 20/06/2014, 07:20

... K, Greinix HT: Long-term outcome and quality of life of patients who are alive and in complete remission more than two years after allogeneic and syngeneic stem cell transplantation Bone Marrow ... V, Lauson J: Development, feasibility and compliance of a web-based system for very frequent QOL and symptom home self-assessment after hematopoietic stem cell transplantation Qual Life Res 2005, ... Bishop MM, Beaumont JL, Hahn EA, Cella D, Andrykowski MA, Brady MJ, Horowitz MM, Sobocinski KA, Rizzo JD, Wingard JR: Late effects of cancer and hematopoietic stem- cell transplantation on spouses...

Ngày tải lên: 20/06/2014, 15:20

... of understanding the functioning of these remarkable stem cells However, current research in stem- cell biology is providing increasing evidence that the behaviors and fates of stem cells are ... Sánchez Alvarado A: Molecular analysis of stem cells and their descendents during cell turnover and regeneration in the planarian Schmidtea mediterranea Cell Stem Cell 2008, 3:327-339 Pfister D, De ... neoblast stem- cell system employed in different animal groups The in vivo cellular dynamics of neoblasts are remarkably similar in platyhelminths and acoels In planarians, macrostomids, and acoels,...

Ngày tải lên: 06/08/2014, 19:21

... hES colony and an cystic body respectively C and D, morphology of DCs differentiated from hES and FL derived CD34+ cells were stained with CD1a and HLA-DR, CD1a and B7.1, and CD1a and B7.2 Results ... differentiating DCs from hES and FL CD34+ cells FACS analysis of differentiating DCs from hES and FL CD34+ cells: CD34+ cells were cultured in cytokine media and analyzed by FACS for CD14 and CD1a markers ... Research and Therapy 2008, 5:1 demonstrated the ability of hES cells to differentiate into a myriad of cell types that include neurons, hematopoietic cells, cardiomyocytes, and insulin-secreting cells,...

Ngày tải lên: 10/08/2014, 05:20