statistical analysis of quantitative rt pcr results

... specificity of the LNA-based method Optimization of primer Tm and high specificity of the PCR reaction is achieved by adding a tail to each of the PCR primers Page of 11 Results MiR-specific qPCR of miRNAs ... 100 ng of RNA in a final volume of 10 μl including μl of 10x poly(A) polymerase buffer, 0.1 mM of ATP, μM of RT- primer, 0.1 mM of each deoxynucleotide (dATP, dCTP, dGTP and dTTP), 100 units of MuLV ... by Exiqon (Denmark) Quantitative PCR Quantitative PCR of biological samples was done in 10 μl total volume with μl of cDNA diluted 8-10 times, μl of 2x QuantiFast SYBR Green PCR master mix (Qiagen,...

Ngày tải lên: 23/03/2014, 22:20

... ces 003 rof C 27 ta noisnetxe lanif dna ces 09 rof C 27 pmet noisnetxe ,ces 54 rof C 06 pmet gnilaenna ,ces 54 rof C 49 ta noitarutaned ,ces 06 rof C 49 ta noitarutaned laitini ,sgniwollof eht ... )RCP-TR( noitcaer niahc esaremylop -esatpircsnart esrever ,)ASU ,hcirdlA amgiS( tnegaer IRT ;swollof sa erew yduts siht rof stnemurtsni dna slacimehC stnemurtsnI dna slacimehC sdohteM dna slairetaM ... tnemtaert BP ,noisulcnoc nI ]8[ sdlof 52- 81 slevel tpircsnart 1H2PYC detavele enoparytem tub dlof 21 yletamixorppa slevel tpircsnart 1H2PYC detavele yletaredom 684-UR dna BP ]71[ noitaluger pu dlof...

Ngày tải lên: 07/08/2014, 18:21

... evaluation of best suited reference gene(s) for normalization of qPCR data in a given set of biological samples Recognizing the importance of reference genes in normalization of RT- qPCR data, ... doi:10.1186/1471-2229-10-49 Cite this article as: Artico et al.: Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT- PCR data BMC Plant ... for experiments of Northern blots and RTqPCRs [26,27] (Table 1) Primers were designed with Primer software [28] using as criterion amplified products from 80 to 180 bp with a Tm of 60 ± 1°C (primer...

Ngày tải lên: 12/08/2014, 03:21

... express the quantitative status of the morphemes of each type as a mass in terminology So the transitions of Pf and Rf, with changing N, express the changes of the status of the morphemes of each ... growth rate of the morphemes of each type observed separately Each of t h e m expresses the probability of encountering a new morpheme for the separate sample consisting of the morphemes of the same ... better descriptions of sub-samples In the following, we use the results of 1000 termlevel random permutations for the empirical descriptions of sub-samples In fact, the results of the term-level...

Ngày tải lên: 20/02/2014, 18:20

... formation of companies for the exploitation of the new opportunities, the setting of the new countries, the exports into and the imports from them, are part of the economic process, as they are part of ... Fundamental Importance of the Historical Approach to the Problems of the Cyclical Process of Evolution B Questions of Principle —A few questions of principle must be disposed of first C The Long ... the element of population are partly motivated by factual propositions and partly by considerations of expository convenience arising out of the purposes of this book It is not, of course, held...

Ngày tải lên: 29/03/2014, 19:20

... shut off Figure shows the STM image of 1.5 ML of InAs WL just prior to QD formation at 300°C Stripes due to As dimers were clearly observed The image was divided by a 25 25 mesh The pitch of the ... each cell of 25 25 mesh in Fig rffiffiffiffi S f ¼ ; N where S is the total area of Voronoi cells, which are not touching the frame, and N is the number of valid reconstruction domains The density of each ... Voronoi tessellations of STM view field of Fig according to a (1 3)/(2 3) domains and b (2 4) domains Table Density, d, and standard deviation of Voronoi cell area, rVc of surface reconstruction...

Ngày tải lên: 21/06/2014, 08:20

... expression is independent of the number of lines This is a consequence of the fact that we are analyzing the average behavior The plot of dmax , as a function of S, is reported in Figure The figure ... results from a campaign of measurements that obviously can suffer some uncertainty level In particular, in our analysis for the case of 10-pair binders, we have used a set of data measurements provided ... the modulus of the diagonal elements of matrix Pk is close to 1, a first choice consists of assuming a midtread quantization law between −1 and However, because of the RWDD property of matrix Hk...

Ngày tải lên: 21/06/2014, 08:20

... performance analysis In this article, the performance of cognitive RSS-WLS approach of [4] in an AWGN channel has been ana2 lyzed by obtaining the closed form expression for mean and covariance of its ... form of the by covariance matrix of estimation error is obtained as, Cv = H − 21M vT D T Ω−1 H − 21M vT D −1 (36) where the first two rows and columns of Cv consist of covariance matrix of positioning ... and covariance of estimation error of cognitive RSS-WLS algorithm is calculated At the end, based on the results of the previous sections, an analytical performance evaluation of cognitive RSS-WLS...

Ngày tải lên: 21/06/2014, 17:20

... observations concerning the results of Lemma are now in order These observations are important because they provide additional insight regarding the role of the rate of Poisson process in modeling ... (ti ) is proportional to λ1/2 , the first term in the power series expansion is of order λ1/2 , the second term is of order 1, the third term is of order 1/λ1/2 , and the kth is term of order (2) ... implementation of the matched filter requires the knowledge of the power spectral density of (1), which is computed in the paper Moreover, in many applications such as filter design and interference analysis, ...

Ngày tải lên: 22/06/2014, 00:20

... properties of the ECDs, the analysis of the statistical behavior of real data can be reduced to the study of the distributions of the scalar variable D The analysis was carried out in terms of ... assuming the ECt model In particular, by resorting to the properties of the EC distributions the authors compared the probability of exceedance (PoE) of the square of the Mahalanobis distance ... statistical analysis of the SIRVs In fact, by assuming perfect knowledge of the mean and covariance matrix of X, the analysis of the SIRV multivariate p.d.f reduces to the study of a univariate p.d.f...

Ngày tải lên: 22/06/2014, 23:20

... software suites are QTL Cartographer (http://statgen.ncsu.edu/qtlcart) and RQTL (http://www.rqtl.org) If statistical tests are needed for mapping, you must need a lot of individuals to map quantitative ... pair of adjacent markers) to perform a genome scan for QTLs The results of a genome scan are depicted graphically, as shown in Figure So mapping QTLs depends crucially on statistical expertise? ... the region of interest These experiments are very laborious and 23.4 Journal of Biology 2009, Volume 8, Article 23 Mackay rarely result in positional cloning of QTLs only a subset of all possible...

Ngày tải lên: 06/08/2014, 19:20

... implementation of IMRT: Report of the IMRT subcommittee of the AAPM radiation therapy committee Med Phys 2003, 30:2089-2115 Doblado FS, Capote R, Rosello JV, Leal A, Lagares JI, Arrans R, Hartmann GH: ... point of measurement (typically less than 1-2 cGy), which might be outside of ion-chamber sensitivity We used gamma criteria of 3%/3 mm for 2D dose analysis of composite plans The percentage of ... implemented IMRT in the clinic and performed DQA measurements over a certain period of time need to analyze their DQA data We confirmed the overall integrity of our IMRT system and established the IMRT...

Ngày tải lên: 09/08/2014, 09:20

... properties of estimators based on mis-specified models For instance, one may be interested in the asymptotic properties of the ML estimator of ( 0’, 6’l’ derived ’, T under the assumption of independence ... estimation of misspecified models Econometrica 50, 1-25 Wright S (1934a) An analysis of variability in number of digits in an inbred strain of guinea pigs Genetics 19, 506-536 Wright S (1934b) The results ... according to sex of calf (males, females) and age of dam at calving in years Scores and were pooled on account of the low frequency of score Nine levels were considered for age of dam: < years,...

Ngày tải lên: 09/08/2014, 18:22

... due to inadequate linkage maps (PARSONS, of 1980a) From an analysis of variance within and between isofemale strains, estimates of the relative proportions of genetic and environmental variances ... (families) is mostly genetic In an analysis of variance (ANOVA), the between-line component of variance contains a quarter of the additive genetic variance and a quarter of the dominance variance, and ... morphological traits, they are of particular value for traits involved in determining the distribution and abundance of organisms (PARSONS, 1983) A major advantage of isofemale strains is that genetic...

Ngày tải lên: 09/08/2014, 22:22

... their encouragement v Baoguang Han STATISTICAL ANALYSIS OF CLINICAL TRIAL DATA USING MONTE CARLO METHODS In medical research, data analysis often requires complex statistical methods where no closed-form ... modelling of the correlation is important to address the issue of dependent censoring of the non-terminal event by the terminal event [2-4] It also can allow modelling of the influence of the nonterminal ... examples of allocation probabilities of BCM in trials that mimic LOTS 82 Figure 4.2 Comparison of the distributions of various re-randomization tests 90 Figure 4.3 Comparison of the...

Ngày tải lên: 24/08/2014, 11:40

... failure of the mapping Thus, one of the fundamental limitations of choosing the anatomy of a single subject as an atlas is the introduction of a statistical bias based on the arbitrary choice of the ... role of Chapter in the ODF-based analysis framework 61 4.2 Illustration of the general framework of the Bayesian HARDI atlas estimation 4.3 63 The evolution of ... xvii 99 LIST OF FIGURES 5.7 Results of geodesic regression for simulated √ ODF data at four noise levels (M = 0.1, 0.5, 1.0, 2.0) against the number of observations n Three types of metric between...

Ngày tải lên: 08/09/2015, 19:24

... as atlas, Iatlas , the space of the initial momentum m0 , provides a linear representation of the nonlinear diffeomorphic shape space in which linear statistical analysis can be applied 21 BACKGROUND ... Hilbert spaces, one can equivalently express the lengths in terms of mt , interpreted as momentum such that for each u ∈ V , mt , u ◦ φt −1 = kV vt , u , where kV is the reproducing kernel of V ... get a proper definition 2.2.2 Conservation Law of Momentum One can prove that mt satisfies the following property at all times [90, 91] Conservation Law of Momentum For all u ∈ V , mt , u = m0 , (Dφt...

Ngày tải lên: 08/09/2015, 19:26

... corticospinal tracts (CST-left, CST-right), using 50 3.5 HARDI Data of Children Brains Figure 3.8: Panels (a-d) show the maps of mean squared error of spherical harmonics coefficients (MSE of ... is worth noting that although LDDMM-Raffelt directly minimizes MSE of SH, LDDMM-ODF achieves a similar average accuracy Nevertheless, for ROI comparisons, we further examine how the MSE of SH ... generation and group analysis of HARDI for a variety of clinical studies 55 DIFFEOMORPHIC METRIC MAPPING OF HIGH ANGULAR RESOLUTION DIFFUSION IMAGING BASED ON RIEMANNIAN STRUCTURE OF ORIENTATION DISTRIBUTION...

Ngày tải lên: 08/09/2015, 19:27

... atlas ODF images in common space Statistical Analysis Biomarkers/ Inference Figure 4.1: The role of Chapter in the ODF-based analysis framework 4.1 General Framework of Bayesian HARDI Atlas Estimation ... a Riemannian structure of the ODFs 62 4.2 The Shape Prior of the Atlas and the Distribution of Random Diffeomorphisms Figure 4.2: Illustration of the general framework of the Bayesian HARDI atlas ... ESTIMATION OF WHITE MATTER ATLAS FROM HIGH ANGULAR RESOLUTION DIFFUSION IMAGING a shape prior of the white matter anatomy and the likelihood of individual observed HARDI datasets First of all, we...

Ngày tải lên: 08/09/2015, 19:27

... harmonics, the results of geodesic regression on ODFs of 4th-order 99 GEODESIC REGRESSION OF ORIENTATION DISTRIBUTION FUNCTIONS WITH ITS APPLICATION TO AGING STUDY √ Figure 5.7: Results of geodesic ... consistency of the results with metrics other than the geodesic distance used in the regression framework, we compared the MSE of the geodesic distance with the MSE of the L2 norm of spherical ... variance of the synthetic data (see the first column) From the results, 98 5.2 Experiments Figure 5.6: Consistency of results under different metrics including the geodesic distance, the L2 norm of...

Ngày tải lên: 08/09/2015, 19:27