stability of proteins and nucleic acids

... fragment of the SAPV was amplified using ul of 16 pmol/ul M13F primer (part of the TOPO vector, upstream of the T7-F primer of the SAPV insert), 0.5 ul of 100 pmol/ul SA-11R primer and 0.5 ul of the ... nucleic acids; (ii) a small molecule ligand or ligands (for example gluthatione), as a nucleic acid label, and an appropriate receptor or protein fragment which binds the ligand as part of the ... specific sequences) for nucleic acid-binding proteins and should also encode corresponding nucleic acid-binding proteins The use of known protein transcription factors and their target DNA sequences...

Ngày tải lên: 11/08/2014, 00:21

... analysis of peptides and proteins N D Udeshi et al dimethylation of Arg, monomethylation, dimethylation and trimethylation of Lys, acetylation and ubiquitination of Lys, and phosphorylation of Ser and ... building blocks of eukaryotic chromatin [15] A host of PTMs reside on the N-terminal tails of the histones, and this array of PTMs has been well documented and includes monomethylation and Fig ETD ... breakthrough in the field of proteomics and enables rapid sequencing of large peptides and intact proteins on a QLT mass spectrometer The scan times required for direct analysis of proteins using ETD...

Ngày tải lên: 18/02/2014, 16:20

... the synthesis of proteins in the presence of other proteins provided in excess at the start of or during the reaction, e.g., for the purpose of rescuing nascently produced insoluble proteins into ... amino acids and the necessity to purify each individual sample In contrast, cell-free systems allow the synthesis of [15N]-labelled proteins with very small quantities of [15N]-amino acids and ... combinations of [15N]-labelled amino acids The most abundant amino acids are labelled in only one of the samples, while the least abundant amino acids are labelled in up to three of the samples...

Ngày tải lên: 07/03/2014, 12:20

... interested in the C r -stability of mixing, and of the rate of mixing, for Axiom A and Anosov flows There is a quite extensive literature on mixing and rates of mixing for certain classes of Anosov flows ... the proofs of Propositions 4.8 and 4.9 are valid for f ∈ C 1,1 Proof of Lemma 4.2 Let Ψ ∈ V be sufficiently C -close to Ψ0 ∈ V∞ and let f ∈ C r (Σ1 ) Statements (1) and (2) of Lemma 4.2, and the ... Remark 1.10) (2) Parts (b) of Theorems 1.3 and 1.6 require only the density part of Lemma 2.2 278 ¨ ¨ MICHAEL FIELD, IAN MELBOURNE, AND ANDREI TOROK Openness and density of good asymptotics In this...

Ngày tải lên: 15/03/2014, 09:20

... at Tm The enthalpy of denaturation DHm of Ngb and horse heart Mb was 72 and 110 kcalÆmol)1, respectively Cytoglobin and the globin of Drosophila have lower values of DHm, 60 and 53 kcalÆmol)1, ... decrease of 20 °C in thermostability, again suggesting a critical role for His E7 in the enhanced thermal stability of Ngb Certain mutations of the distal histidine in Mb and Hb lead to instability ... native Ngb had negative bands at 208 and 222 nm (Fig 1), as expected for a high percentage of alpha helix Analysis of the secondary structure of Ngb gave 78% alpha helix and 22% of other forms, similar...

Ngày tải lên: 30/03/2014, 16:20

... cleavage and C18 RP-HPLC has two additional amino acids (Gly and Ser) at the N terminus and a theoretical mass of 10307.6 Daltons (16) A detailed account of the expression and characterization of rCP10 ... (e.g., PicoFuge, Stratagene, Palo Alto, CA) Methods 3.1 In-Gel Digestion (see Notes and 9) 3.1.1 Excision of Protein Bands (spots) from Gels Rinse the entire gel with water and excise bands of interest ... dishes, and so on) may acquire a static charge and attract dust Accumulation of even a minute amount of dust in solutions and reagents results in massive contamination of samples with human and sheep...

Ngày tải lên: 11/04/2014, 09:50

... cause hydrolysis of fatty acids, oxidation of the unsaturated olefinic bonds, release of iron, and increase nonhydratable phospholipids All of these changes affect quality and stability, so the ... testing of oils and fat-containing foods Number of Samples The number of samples that can be evaluated in one session is dependent on the nature of the product and on the amount of training and experience ... determining moisture, the “Swift stability test” bath, and the analytical balance A set of calibrated weights from the National Bureau of Standards were used, and tenths of milligrams were calculated...

Ngày tải lên: 23/05/2014, 12:34

... defined as in Theorem 2.2 Proof It follows from the assumptions that ϕ 0, 0, and so f is similar to the proof of Theorem 2.2 and we omit the details The rest of the proof Corollary 2.6 Let p, q, ... Proceedings of the National Academy of Sciences of the United States of America, vol 27, pp 222–224, 1941 T Aoki, “On the stability of the linear transformation in Banach spaces,” Journal of the Mathematical ... Journal of Inequalities and Applications 12 A Najati and M B Moghimi, Stability of a functional equation deriving from quadratic and additive functions in quasi-Banach spaces,” Journal of Mathematical...

Ngày tải lên: 22/06/2014, 02:20

... destination (37% of the proteins; decreased volume in 66% of the proteins of this group was observed) Most of the proteins associated with dormancy breaking in the presence of ABA (Fig 3B) were ... pattern of 71 proteins, with a shift away from accumulation of a major group of proteins, involved mainly in energy and protein metabolism [5] The same negative effect of ABA was observed on proteins ... deficit and storage [24] Proteomic analysis of the seeds of another woody plant, Prunus campanulata, provide evidence of the involvement of prunin and dehydrin in the response to warm and cold...

Ngày tải lên: 12/08/2014, 03:20

... induction of non-transcriptional responses to nucleic acids A common feature of the recognition of non-self nucleic acids by TLRs, RLRs and DAI is their ability to induce robust activation of genes ... localization of nucleic acid-specific TLRs is important in preventing their contact with self nucleic acids [5] TLR9 was the first TLR identified to interact with nucleic acids, and its classical ligand ... to pDCs and B cells, where the nucleic acids can activate TLR7 and/ or TLR9, resulting in the secretion of type Volume 9, Issue 3, Article 211 Chi and Flavell 211.5 I interferons by pDCs and the...

Ngày tải lên: 14/08/2014, 08:20

... analyses of single protein and simple protein complex samples were mainly conducted on MALDI-TOF, MALDI-TOF/TOF, ESI-TOF (electrospray ionization-TOF) and ESI-Q-TOF (ESI-linear trap quadrupole-TOF) ... proteomics: Analysis of proteins and protein complexes by chemical cross-linking and mass spectrometry Zhuo A Chen Thesis for the Degree of Doctor of Philosophy The University of Edinburgh August ... of Pol II and Pol II-TFIIF complexes 42 44 2.2.1 The Pol II complex and the Pol II-TFIIF complex 44 2.2.2 Cross-linking titration of Pol II and Pol II-TFIIF complexes 45 2.2.3 Cross-linking of...

Ngày tải lên: 30/09/2015, 05:51

... studies of proteins both in vitro and in vivo v List of Tables Table Page The influence of C-terminal residues on the in vivo cleavage of EGFP-intein and on-column cleavage/biotinylation of EGFP ... Biotinylation of three model proteins 24 3.2.1 Cloning of target genes into pTYB1 expression vector 24 3.2.2 Expression and extraction of fusion proteins 25 3.2.3 Affinity purification and on-column ... C-terminus of the yeast gene A standard PCR mixture contained 1x HotStarTaq DNA polymerase buffer (Qiagen), 0.2 mM of each dNTPs (NEB), 0.5 µM of each primer, 100 ng of plasmid DNA template and units of...

Ngày tải lên: 08/11/2015, 16:30

... sparger, and numerous inlets and outlets for removal of samples and medium and for adding various solutions to the growing culture These include nutrients and growth factors to support growth and ... General layout of the bacterial cultivation process for production of proteins and other biological products 10.2.2 Instrumentation and Infrastructure Required To conduct a process of recombinant ... to these elements, cells need phosphorus for the formation of RNA and DNA, and sulfur for the formation of the amino acids methionine and cysteine Other trace elements, such as metal ions, are...

Ngày tải lên: 24/04/2016, 10:44

... Structural and genomic correlates of hyperthermostability J Biol Chem 275, 32383–32386 Thermodynamic stability and folding of proteins Das F & Gerstein M (2000) The stability of thermophilic proteins: ... Thermodynamic stability and folding of proteins K A Luke et al Conclusions We have summarized the in vitro data that exist on thermodynamic stability and folding ⁄ unfolding reactions of proteins ... ª 2007 FEBS K A Luke et al Thermodynamic stability and folding of proteins heptamer, and final folding takes place [30] The high thermodynamic stability of the folded Aacpn10 monomer [24] can explain...

Ngày tải lên: 07/03/2014, 05:20

... sequences of CgueCMV-1.1 and CgueCMV-1.2 (B) Pairwise alignments of the UL56 and gB proteins and the partial DPOL proteins of CgueCMV-1.1 and CgueCMV-1.2 are shown Dots represent identical amino acids, ... (gB), and two thirds of the ORF UL54 (DPOL) (Figure 6B) The nucleotide and amino acid sequence differences of CgueCMV-1.1 and CgueCMV-1.2 were 0.6% and 0.5% (UL56), 20% and 8% (UL55) and 1% and ... sequence C Figure LNA-based and bigenic amplification of beta- and gammaherpesviruses LNA-based and bigenic amplification of beta- and gammaherpesviruses Schematic diagram of the analysis strategy...

Ngày tải lên: 18/06/2014, 18:20

... Detection of nucleic acids Further, three carbon composite electrodes with different content of carbon particles with various shapes and sizes were prepared and tested on measuring of nucleic acids ... versatility of the electrode was tested Cyclic voltammetry was used for the detection of two nucleic acids samples mentioned above and the basic electrochemical behaviour of nucleic acids at the ... suitable tool for detection of nucleic acids We show the successful application of modern nano-technologies not only for detecting of nucleic acids but also for distinguishing of all bases signals Methods...

Ngày tải lên: 21/06/2014, 03:20

... sources of contamination in PCR-based studies of XMRV: (i) MLV-encoding nucleic acids present in commercial PCR reagents, (ii) trace amounts of mouse genomic DNA in human blood and tissue samples, and ... claims of MLV-related viruses in prostate cancer and CFS patients Future assessments of the prevalence of XMRV should include more rigorous PCR and phylogenetic tests to exclude the possibility of ... Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses Retrovirology 2010 7:112 Submit your next manuscript to BioMed Central and...

Ngày tải lên: 13/08/2014, 01:20

... Structure of RPA Figure 5: NMR structure of XPA Figure 6: Identification of SMIs of RPA Figure 7: Structures of SMIs of RPA Figure 8: In vitro analysis of TDRL-505 Figure 9: Cellular analysis of TDRL-505 ... disruption of the maintenance of genomic stability has deleterious consequences as seen in the acquisition of mutations and eventual development of disease; however inhibition of genomic stability ... irradiation and chemical mutagens (4) The repair of bulky DNA damage is initiated by a damage recognition step and assembly of a pre-incision complex, followed by excision of the damaged strand and gap-filling...

Ngày tải lên: 24/08/2014, 11:28

... Hybridization and Detection of Nucleic Acids 1.1.1 Basic of nucleic acid hybridization The nucleic acid is a polymer consists of monomers called nucleotides Each nucleotide composes of a nitrogenous ... .142 Table 4.3 Typical concentration and purity of p185-ssDNA standard 144 xii LIST OF FIGURES Figure 1.1 Basic principle of nucleic acids hybridization and detection on a solid surface ... .1 1.1 Hybridization and Detection of Nucleic Acids 1.1.1 Basic of nucleic acid hybridization .1 1.1.2 Label-free detection of nucleic acids 1.1.2.1 Electrochemical ...

Ngày tải lên: 12/09/2015, 09:28