stability of plant and microbial phytases

... interested in the C r -stability of mixing, and of the rate of mixing, for Axiom A and Anosov flows There is a quite extensive literature on mixing and rates of mixing for certain classes of Anosov flows ... the proofs of Propositions 4.8 and 4.9 are valid for f ∈ C 1,1 Proof of Lemma 4.2 Let Ψ ∈ V be sufficiently C -close to Ψ0 ∈ V∞ and let f ∈ C r (Σ1 ) Statements (1) and (2) of Lemma 4.2, and the ... Remark 1.10) (2) Parts (b) of Theorems 1.3 and 1.6 require only the density part of Lemma 2.2 278 ¨ ¨ MICHAEL FIELD, IAN MELBOURNE, AND ANDREI TOROK Openness and density of good asymptotics In this...

Ngày tải lên: 15/03/2014, 09:20

... at Tm The enthalpy of denaturation DHm of Ngb and horse heart Mb was 72 and 110 kcalÆmol)1, respectively Cytoglobin and the globin of Drosophila have lower values of DHm, 60 and 53 kcalÆmol)1, ... decrease of 20 °C in thermostability, again suggesting a critical role for His E7 in the enhanced thermal stability of Ngb Certain mutations of the distal histidine in Mb and Hb lead to instability ... native Ngb had negative bands at 208 and 222 nm (Fig 1), as expected for a high percentage of alpha helix Analysis of the secondary structure of Ngb gave 78% alpha helix and 22% of other forms, similar...

Ngày tải lên: 30/03/2014, 16:20

... references of laws, standards, and codes of practice, as they are updated frequently All of the costs and examples have been put on a U.S basis, and examples have been provided in both metric and conventional ... of international standards The European countries used to maintain their own national standards, but these are now being superseded by common European standards Lists of codes and standards and ... Capacity 92 Enthalpy of Mixtures 94 Enthalpy-Concentration Diagrams 95 Heats of Reaction 98 Standard Heats of Formation 101 Heats of Combustion 102 Compression and Expansion of Gases 104 Energy...

Ngày tải lên: 01/04/2014, 11:33

... testing of oils and fat-containing foods Number of Samples The number of samples that can be evaluated in one session is dependent on the nature of the product and on the amount of training and experience ... Historical Glimpses of Analytical and Quality Assessment Methods for Fats and Oils H.J Dutton Factors Affecting Oil Quality and Stability T.H Smouse Sensory Analyses of Oils and Fat-Containing ... determining moisture, the “Swift stability test” bath, and the analytical balance A set of calibrated weights from the National Bureau of Standards were used, and tenths of milligrams were calculated...

Ngày tải lên: 23/05/2014, 12:34

... defined as in Theorem 2.2 Proof It follows from the assumptions that ϕ 0, 0, and so f is similar to the proof of Theorem 2.2 and we omit the details The rest of the proof Corollary 2.6 Let p, q, ... Proceedings of the National Academy of Sciences of the United States of America, vol 27, pp 222–224, 1941 T Aoki, “On the stability of the linear transformation in Banach spaces,” Journal of the Mathematical ... Journal of Inequalities and Applications 12 A Najati and M B Moghimi, Stability of a functional equation deriving from quadratic and additive functions in quasi-Banach spaces,” Journal of Mathematical...

Ngày tải lên: 22/06/2014, 02:20

... prices “over and beyond” what such changes imply for the path of aggregate demand and inflation (Borio and Lowe 2002, Cecchetti et al 2000) Proponents of this policy emphasise that episodes of financial ... precision of the estimates, we thus follow Goodhart and Bean (2004) and Kohn (2006) discuss the implications of lags for the use of monetary policy in the face of asset price bubbles Sutton (2002) and ... for Denmark, Switzerland, Spain, Finland, France, Germany, Ireland, Italy, the Netherlands, Norway and the UK, a threemonth Treasury bill rate for Belgium, Sweden and the US, and a three-month commercial...

Ngày tải lên: 24/10/2012, 09:40

... tr.106-108 Ashley D.A., Doss B.D., and Bennett O.L (1965) Relation of cotton leaf area index to plant growth and fruiting, Agronomy Journal 57, pp 61-64 Gomez K Wanchai A and Gomez Arturo A (1984) Statistical...

Ngày tải lên: 28/08/2013, 14:15

... any SOFC system, the performance of the INER SOFC system is dependent not only on the design and operating conditions of the fuel cell stack, but also on the design and operating conditions of ... provided by the anode off-gas given an anode off-gas temperature of less than 650 oC, a cathode off-gas temperature of less than 390 oC, and a flame barrier temperature of less than 700 oC However, ... SOFC BOP system (S/C=1.7, O/C=0.3, fuel utilization = 64.2%, electrical conversion efficiency = 44%) Design of SOFC BOP components The BOP components of a SOFC system account for between 50% and...

Ngày tải lên: 05/09/2013, 16:10

... Digestion Support Software, Cork Institute of Technology, 2007 [7] Barelli D., Csambalik L., Mestas C and Santos D Economical and Environmental Analysis of a Biogas Plant within the Context of a Real ... same cannot be said of the residential apartment of lecturers and other staff of the university Figure Feeds into the sewage treatment plant After the sewage enters the treatment plant, it goes through ... Journal of Energy and Environment (IJEE), Volume 1, Issue 6, 2010, pp.1009-1016 Figure Layout of KNUST sewage treatment plant Methodology Sample of the sewage from the PST was collected and analysed...

Ngày tải lên: 05/09/2013, 16:11

... evolution of specific day-length and temperature requirements for plant development meant adaptation of specific plants to particular latitudes Accordingly, exploitation of "useful" plants (and of domesticatable ... together with common plant names of well-known plants, plant family and the plant part involved and (c) the biochemical target being considered, a measure of the affinity of the compound for the ... chemistry of living things) This biochemical review provides a detailed background for understanding the nature and function of the targets of plant defensive metabolites and proteins The remainder of...

Ngày tải lên: 17/02/2014, 05:20

... absence and presence of NaCl and CaCl2 at pH 5.5 and 23 °C are compared The results show that the instability of PLDa2 is Table Influence of salts on the observed first-order rate constants (kobs) of ... Structure of plant phospholipase D S Stumpe et al from the ratio of the forward scattering intensity of the sample and that of the molecular mass standard BSA The ab initio deconvolution of the ... (near-UV) and 0.1 mm (far-UV) quartz cuvettes by scanning at 20 nmÆmin)1 with a resolution of 0.1 nm, a response time of s, a bandwidth of nm, and a temperature of 20 °C An average of 10 scans...

Ngày tải lên: 19/02/2014, 02:20

... assembly and disassembly In this work, we studied the effects of chemical (i.e urea) and physical (i.e high pressure) denaturing agents in the structure and stability of the MS2 bacteriophage, and of ... fraction Fig Dissociation and denaturation of wild-type (WT) and mutant MS2 particles (A) Light scattering and spectral center of mass measurements of WT MS2 as a function of urea concentration To ... unaffected and showed only a 10-fold loss of titer Pressure stability of the MS2 bacteriophage and ts mutants Fig High-performance gel filtration chromatography of bacteriophage MS2 and the temperature...

Ngày tải lên: 19/02/2014, 12:20

... isoforms by the excision of the prosegment and of most of the PSI [21] Conversely to what has been found in vivo [31], heavy and light chains of the processed forms of recombinant cyprosin are ... N-terminal of the mature enzyme and by the ÔflapÕ The anchorage of the prosegment and of part of the N-terminus in the active site cleft is made by ionic interactions established between Lys11/Tyr13 of ... mechanisms of degradation of pathogenesis-related proteins in plants Plant Physiol 95, 616–622 Guevara, M.G., Oliva, C.R., Machinandiarena, M & Daleo, G.R (1999) Purification and properties of an aspartic...

Ngày tải lên: 19/02/2014, 12:20

... vant Hoff analysis and those determined by uorescence and DSC (see before) Determination of the Th and Ts The values of the temperatures where the enthalpy and entropy are zero, Th and TsÂ, ... The tting parameters for the cold and heat-denaturation data of 2.5 and 3.0 M urea are listed in Table 1, and Fig 5B ể FEBS 2004 Conformational stability of HPr of S coelicolor (Eur J Biochem 271) ... 14ị which are similar to Eqns 5, and 7, respectively Here, mmiDHi and mmiDSi are the values of mDHi and mDSi at the ể FEBS 2004 Conformational stability of HPr of S coelicolor (Eur J Biochem 271)...

Ngày tải lên: 19/02/2014, 12:20

... (fragmentation: m/z 317.3, 305.2, 291.1, 253.2 and 240.1), 350.2 (fragmentation: m/z 333.2 and 307.1) and 431.3 (fragmentation: m/z 414.3, 388.3 and 337.1) MALDI-TOF-MS of the separated peak provided a single ... 18 19 20 21 and oxidative half-reactions and the role of copper ions in plant and mammalian copper-amine oxidases Eur J Inorg Chem 1, 35–42 Medda, R., Padiglia, A & Floris, G (1995) Plant copper-amine ... oxidized by an excess of GPAO (500 nkat) in 0.1 M potassium phosphate buffer, pH 7.0, in the presence of catalase (200 U) and 2.5 mM ABA After h of incubation at 30 °C, mL of 15% trichloroacetic...

Ngày tải lên: 19/02/2014, 16:20

... half-lives of 22 and 23 h for wild type GAT1 in the absence and presence of dMM, respectively, and of 12 and 5.5 h for the DDN and DDQ mutants, respectively GABA values of NNN treated with dMM, and of ... trimming of N-glycans is involved in the regulation of the stability and trafficking of GAT1 It has been reported that a variety of molecular chaperones and folding enzymes assist the folding of newly ... activities of the N-glycosylation 1628 Fig Determination of expression of GFP-tagged GAT1 mutants on the surface of transfected CHO cells and measurement of GABA-uptake by GFP-tagged GAT1 wild type and...

Ngày tải lên: 19/02/2014, 17:20

... shows the fluorescence spectra of wild-type SNase and the four mutants E142O, K133A, W140A and W140O The fluorescence spectra of E142O and K133A are similar to that of the wild-type protein However, ... at 140 (W140A and W140O) was much lower than both the wild-type protein and mutants E142O and K133A Thermal analysis of protein unfolding The DSC curves of the wild-type protein and the mutants ... wild-type and mutant SNase Spectra of five proteins [wild-type (WT), W140A, E140O, W140O and K133A] Protein concentration was 0.4 mgÆmL)1 Fig Calometric melting curves of wild-type and mutants of SNase...

Ngày tải lên: 20/02/2014, 01:20

... analysis of the consequent hits was also performed to investigate the simultaneous improvement of the activity and stability of Symbiobacterium toebii TPL, as a better structural understanding of how ... basis of a co-improvement in stability and activity For example, the T1 ⁄ values for AS4 and AS6 were 6.7 and 11.2 °C higher, respectively, and the catalytic activities were improved by 2.8- and ... mutations from both the stability and activity mutants, and demonstrated a significant improvement in both stability and catalytic activity (Fig 1) As such, the activity and stability mutation combinations...

Ngày tải lên: 07/03/2014, 00:20

... characterization of Ngb and Mb at neutral and acidic pH (A) Far-UV CD spectra of human holoNgb and apoNgb dissolved in 20 mM Tris ⁄ HCl and 0.15 M NaCl (pH 7.0) (B) Far-UV CD spectra of human apoNgb and horse ... acid denaturation of Mb, Ngb and H64Q mutant of Ngb was followed by measuring the decrease in intensity of the Soret band (Ngb, kmax of 412 nm; H64Q, kmax of 406 nm; Mb, kmax of 409 nm) in the ... concentrations of urea The urea denaturation of the holo forms of Ngb (open circles) and Mb (open triangles) was performed in 20 mM Tris ⁄ HCl and 0.15 M NaCl (pH 7.0), and that of apoNgb (filled...

Ngày tải lên: 07/03/2014, 02:20

... (2005) Effects of submersion and aerial exposure on clutches and hatchlings of Pomacea canaliculata (Gastropoda: Ampullariidae) Am Malacol Bull 20, 55–63 Structure and pH stability of snail egg ... to be unpalatable [29], and in fact have a small number of predators The pH stability of ovorubin is within the pH range of vertebrate digestive tract fluids [30,31], and the present results indicate ... 4523 Structure and pH stability of snail egg ovorubin M S Dreon et al B A Y X X X Z Y C D log I (Q) 10 0.1 0.1 0.2 Q (Å–1) Fig Three-dimensional model of ovorubin from the eggs of P canaliculata,...

Ngày tải lên: 07/03/2014, 06:20