... thequantitation of microgram quantities of protein utilizing theprinciple of protein-dye binding. Anal. Biochem. 72, 248–254.20. Laemmli, U.K. (1970) Cleavage of structural proteins during theassembly of ... protein belonging to the GTP-binding elongation factor family, which promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis and the capture of deacylated ... quantify the amount of recoveredprotein owing to the presence of a high molar excess of BSA remaining in the buffer. Therefore, a fixed aliquot of the protein was incubated with the indicated probes,...
... category of symptomatics.DISCUSSIONThe findings of the three surveys showedthe relative importance of cough as a predominantsymptom employed in screening the population. In fact, two screening ... weeks or more as defined in RNTCP. Thisindicated the importance of identifying symptomaticsemploying cough of two weeks or more instead threeweeks or more for diagnosis of TB. Among the55561 ... patienthaving cough of three weeks or more with orwithout other symptoms. The importance of including quality check in the survey employingsymptom inquiry was well demonstrated in an earlierreport12...
... of increase in LV dyssynchrony during dobutamine stress in patients with wide QRS duration (20). In contrast, Kurita et al. reported on an increased mechanical dyssynchrony during pacing–induced ... time in IDCM patients yielded the following findings: LBBB was a major determinant of the duration of LV systole, irre-spective of the presence of left ventricular disease. The effect of the ... hemodynamics The patients performed supine bicycle exercise beginning at a load of 25 watt with increases of 25 watt every 5 min until the development of fatigue or limiting dyspnoea. Rest and exercise...
... were interrupted by twointrons located in identical positions (Fig. 1B). The firstintron included nucleotides 50–96 and the second intronincluded nucleotides 210–253 in each gene. Excludingintrons ... longitudinal interactions formed by c-T1 and c-T2.Finally, c-T2 possesses a glycine at position 72 in place of consensus ⁄ c-T1 arginine (Fig. 4B¢). This substitutionmay ‘open’ the nucleotide-binding ... whereas the level of the c-T1 transcriptremained unchanged. Ninety-eight percent of the cellswere undergoing mitosis ⁄ cytokinesis at this time (asdetermined by counting of cells using a stereomicro-scope)....
... andconsisted of a linker (Glc-Kdo2)-lipid A structure. Thephenotype of this mutant was examined to investigatethe roles ofdifferent moieties of the LOS molecule in biological activities of the ... (10 lLof106colony forming units, CFU) were inoculated into 190 lLreaction wells containing the diluted normal human serum,25% of heat-inactivated normal human serum, or DPBSGalone and incubated ... chromosomal DNA of M. catarrhalis strain O35E using primers 25 and 26(Table 1, Fig. 2). The PCR product was cloned intopCR2.1 using a TOPO TA cloning kit (Invitrogen, Carls-bad, CA) to obtain pCRlgt3....
... Co-stimulation of T cells with CD2 augments T CR-CD3-mediated activation of protein tyrosine kinase p72syk, resulting in incresed tyrosinephosphorylation of adapter proteins, Shc and Cbl. Int. Immunol.10, ... containing th e GAL4 DNA-binding domain and the c-Ju n activation domain (residues1–223) in wild-type and mutant forms ( not phosphorylat-able by mutation of both serines 63 and 73 to alanine).These ... fthese protein interactions, tetraspanin antigens can in uenceintracellular signalling pathways. The ligation o f CD53 hasbeen shown to induce intracellular calcium mobilization in different...
... of microgram quantities of proteinutilizing the principle of protein-dye binding. AnalBiochem 72, 248–254.40 Laemmli UK (1970) Cleavage of structural proteinsduring the assembly of head of ... observed in thecase of the superimposed PKV–RKV models, whosemajor nonoverlapping regions are, instead, localized in the H-site of the enzymes (Fig. 2B). These findings are in agreement with the different ... themaintenance of a high-affinity CDNB-binding site. However, the substitu-tion E117K gives rise to mutants showing increased kcatvalues forCDNB, suggesting that Lys117 might positively in uence...
... importin b binding (IBB)domain in its N-terminus and the C-terminal NLS-binding domain. In the absence of importin b,anauto-inhibiting part of the IBB domain forms an intra-molecular interaction ... peptide of the cargo binds in extended conformation to the binding pockets of the superhelical surface of importin a. These bindingpockets contain several conserved residues (e.g. aspara-gine, ... oxygenatoms of conserved amino acids within importin a (themain-chain oxygen of glycine at position 168, the side-chain hydroxyl oxygen of threonine at position 173, andthe side-chain carboxylate...
... such as vitamin A, vitamin E diminish the lipidcontent of blood plasma in patients with non-insulin-dependentdiabetes mellitus (Lee et al., 2007a). The evaluation of GABA in germinated brown ... Administration,Republic of Korea.ReferencesAbdullah, A., Baldwin, R. E., & Minor, H. (1984). Germination effect on flatus causingfactors and antinutrients of mungbeans and two strains of ... becomes one of the focuses in the field of func-tional food research. Change in the GABA content is enhanced in the germination state, so allowing time for germination duringprocessing can help...
... microdomains (GEM) of the plasma membraneand to be involved in the regulation of epidermal growthfactor receptor (EGFr or ErbB1) activity. To gain furtherinsight into the mechanisms involved in ... masses) of recombinant proteins are indicated.(B) Protein profile of the gradient, visualized by Ponceau S staining of the nitrocellulose membrane.Ó FEBS 2004 EGF receptors distribution in cell ... microscopyCellsgrownincoverslipswerewashedinNaCl/Pi,fixedinacetone at )20 °C for 7 min, and incubated in NaCl/Picontaining 3% (w/v) BSA4for 1 h at 37 °Ctoblocknonspecific binding sites. Coverslips...
... θ of an individual at a given point of time in terms of theprevailing age t, individual background characteristics x, current conditions z(τ),exogenous business-cycle indicators c(τ − t) of ... probability of twinbirths (see Subsection 4.1.2) is in line with our finding that twinning ratesare slightly lower in recessions.18Some other possible explanations seem less likely. If inferior housing ... Press,Durham.351 Introduction In many scientific disciplines, the interest in long-run effects of early-life con-ditions has been strongly increasing during the past years. In medical science,the...
... modifica-tions of the side chains of amino acids within a protein. In particular, the side chains of cysteine, histidine, methionine,tryptophan and tyrosine are susceptible to ROS-inducedmodifications ... (1999)Photodynamic crosslinking of proteins. Kinetics of the FMN-and rose bengal-sensitized photooxidation and intermolecularcrosslinking of model tyrosine-containing N-(2-hydroxypropyl)methacrylamide ... 4A,B).Matching the blot with the protein map shows that PDTcaused labeling of a-tubulin and b-tubulin (spots 1 and 2).The minor spot 3 probably reflects labeling of a smallfraction of actin. The...
... importance of the positioning in the membrane. When changing the two tryptophans(residue 48 and 56) of penetratin to phenylalanines it wasshown that the translocating property of penetratin wasessentially ... differ-ence in chemical shift observed for Phe49 in penetratin and in penetratin(W48F,W56F) is most likely due to changes in ring currents when changing amino acid 48 from atryptophan to a phenylalanine, ... average size of the head-group region of the bilayer. Onlyeffects that result in a remaining amplitude of <0.4 are shown. Bluecolor indicates the effect of Mn2+ions, light red the effect of 1-palmitoyl-2-stearoyl-sn-glycero-5-doxyl-3-phosphatidylcholine,...
... E.(1994) Binding sites of quinones in photosynthetic bacterialreaction centers investigated by light- induced FTIR differencespectroscopy: binding of chainless symmetrical quinones to theQAsite of ... (1994) Binding sites of quinones in photosynthetic bacterialreaction centers investigated by light- induced FTIR differencespectroscopy: assignment of the interactions of each carbonyl of QA in Rhodobacter ... stretching vibration of QAby 60 cm)1[10,11], indicating strong asymmetricbinding of UQ10at the QAsite, in contrast to symmetric,weaker binding of UQ10at the QBsite [12,13].To explain...
... identifi-cation of two light- dependent protein kinases involved in phosphorylation of thylakoid proteins. STN7 pro-tein kinase is essential for phosphorylation of Lhcb1,Lhcb2 and Lhcb4 proteins [11,17] ... conditions, including the process of leaf senes-cence in Arabidopsis [31].The C-terminus of CaS contains also a motif forinteraction with 14-3-3 proteins and FHA domains,according to eukaryotic linear ... motifthat is involved in interaction with 14-3-3 proteins andproteins with the ‘forkhead-associated’ (FHA) domain.These domains are found in a variety of signaling pro-teins, and can bind directly...