speed of different colours of light in vacuum

... the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254. 20. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of ... protein belonging to the GTP- binding elongation factor family, which promotes the GTP- dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis and the capture of deacylated ... quantify the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the buffer. Therefore, a fixed aliquot of the protein was incubated with the indicated probes,...

Ngày tải lên: 21/02/2014, 00:20

... category of symptomatics. DISCUSSION The findings of the three surveys showed the relative importance of cough as a predominant symptom employed in screening the population. In fact, two screening ... weeks or more as defined in RNTCP. This indicated the importance of identifying symptomatics employing cough of two weeks or more instead three weeks or more for diagnosis of TB. Among the 55561 ... patient having cough of three weeks or more with or without other symptoms. The importance of including quality check in the survey employing symptom inquiry was well demonstrated in an earlier report 12 ...

Ngày tải lên: 06/03/2014, 04:20

Ngày tải lên: 20/06/2014, 08:20

... of increase in LV dyssynchrony during dobutamine stress in patients with wide QRS duration (20). In contrast, Kurita et al. reported on an increased mechanical dyssynchrony during pacing–induced ... time in IDCM patients yielded the following findings: LBBB was a major determinant of the duration of LV systole, irre- spective of the presence of left ventricular disease. The effect of the ... hemodynamics The patients performed supine bicycle exercise beginning at a load of 25 watt with increases of 25 watt every 5 min until the development of fatigue or limiting dyspnoea . Rest and exercise...

Ngày tải lên: 05/11/2012, 11:32

... were interrupted by two introns located in identical positions (Fig. 1B). The first intron included nucleotides 50–96 and the second intron included nucleotides 210–253 in each gene. Excluding introns ... longitudinal interactions formed by c-T1 and c-T2. Finally, c-T2 possesses a glycine at position 72 in place of consensus ⁄ c-T1 arginine (Fig. 4B¢). This substitution may ‘open’ the nucleotide-binding ... whereas the level of the c-T1 transcript remained unchanged. Ninety-eight percent of the cells were undergoing mitosis ⁄ cytokinesis at this time (as determined by counting of cells using a stereomicro- scope)....

Ngày tải lên: 07/03/2014, 04:20

... and consisted of a linker (Glc-Kdo 2 )-lipid A structure. The phenotype of this mutant was examined to investigate the roles of different moieties of the LOS molecule in biological activities of the ... (10 lLof10 6 colony forming units, CFU) were inoculated into 190 lL reaction wells containing the diluted normal human serum, 25% of heat-inactivated normal human serum, or DPBSG alone and incubated ... chromosomal DNA of M. catarrhalis strain O35E using primers 25 and 26 (Table 1, Fig. 2). The PCR product was cloned into pCR2.1 using a TOPO TA cloning kit (Invitrogen, Carls- bad, CA) to obtain pCRlgt3....

Ngày tải lên: 07/03/2014, 05:20

... Co-stimulation of T cells with CD2 augments T CR-CD3-mediated activation of protein tyrosine kinase p72syk, resulting in incresed tyrosine phosphorylation of adapter proteins, Shc and Cbl. Int. Immunol. 10, ... containing th e GAL4 DNA- binding domain and the c-Ju n activation domain (residues 1–223) in wild-type and mutant forms ( not phosphorylat- able by mutation of both serines 63 and 73 to alanine). These ... f these protein interactions, tetraspanin antigens can in uence intracellular signalling pathways. The ligation o f CD53 has been shown to induce intracellular calcium mobilization in different...

Ngày tải lên: 08/03/2014, 22:20

... of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254. 40 Laemmli UK (1970) Cleavage of structural proteins during the assembly of head of ... observed in the case of the superimposed PKV–RKV models, whose major nonoverlapping regions are, instead, localized in the H-site of the enzymes (Fig. 2B). These findings are in agreement with the different ... the maintenance of a high-affinity CDNB-binding site. However, the substitu- tion E117K gives rise to mutants showing increased k cat values for CDNB, suggesting that Lys117 might positively in uence...

Ngày tải lên: 15/03/2014, 09:20

... importin b binding (IBB) domain in its N-terminus and the C-terminal NLS- binding domain. In the absence of importin b,an auto-inhibiting part of the IBB domain forms an intra- molecular interaction ... peptide of the cargo binds in extended conformation to the binding pockets of the superhelical surface of importin a. These binding pockets contain several conserved residues (e.g. aspara- gine, ... oxygen atoms of conserved amino acids within importin a (the main-chain oxygen of glycine at position 168, the side- chain hydroxyl oxygen of threonine at position 173, and the side-chain carboxylate...

Ngày tải lên: 15/03/2014, 11:20

... such as vitamin A, vitamin E diminish the lipid content of blood plasma in patients with non-insulin-dependent diabetes mellitus (Lee et al., 2007a). The evaluation of GABA in germinated brown ... Administration, Republic of Korea. References Abdullah, A., Baldwin, R. E., & Minor, H. (1984). Germination effect on flatus causing factors and antinutrients of mungbeans and two strains of ... becomes one of the focuses in the field of func- tional food research. Change in the GABA content is enhanced in the germination state, so allowing time for germination during processing can help...

Ngày tải lên: 15/03/2014, 15:42

... microdomains (GEM) of the plasma membrane and to be involved in the regulation of epidermal growth factor receptor (EGFr or ErbB1) activity. To gain further insight into the mechanisms involved in ... masses) of recombinant proteins are indicated. (B) Protein profile of the gradient, visualized by Ponceau S staining of the nitrocellulose membrane. Ó FEBS 2004 EGF receptors distribution in cell ... microscopy CellsgrownincoverslipswerewashedinNaCl/P i ,fixedin acetone at )20 °C for 7 min, and incubated in NaCl/P i containing 3% (w/v) BSA 4 for 1 h at 37 °Ctoblock nonspecific binding sites. Coverslips...

Ngày tải lên: 16/03/2014, 16:20

... θ of an individual at a given point of time in terms of the prevailing age t, individual background characteristics x, current conditions z(τ), exogenous business-cycle indicators c(τ − t) of ... probability of twinbirths (see Subsection 4.1.2) is in line with our finding that twinning rates are slightly lower in recessions. 18 Some other possible explanations seem less likely. If inferior housing ... Press, Durham. 35 1 Introduction In many scientific disciplines, the interest in long-run effects of early-life con- ditions has been strongly increasing during the past years. In medical science, the...

Ngày tải lên: 17/03/2014, 08:20

... modifica- tions of the side chains of amino acids within a protein. In particular, the side chains of cysteine, histidine, methionine, tryptophan and tyrosine are susceptible to ROS-induced modifications ... (1999) Photodynamic crosslinking of proteins. Kinetics of the FMN- and rose bengal-sensitized photooxidation and intermolecular crosslinking of model tyrosine-containing N-(2-hydroxypropyl) methacrylamide ... 4A,B). Matching the blot with the protein map shows that PDT caused labeling of a-tubulin and b-tubulin (spots 1 and 2). The minor spot 3 probably reflects labeling of a small fraction of actin. The...

Ngày tải lên: 23/03/2014, 15:21

... importance of the positioning in the membrane. When changing the two tryptophans (residue 48 and 56) of penetratin to phenylalanines it was shown that the translocating property of penetratin was essentially ... differ- ence in chemical shift observed for Phe49 in penetratin and in penetratin(W48F,W56F) is most likely due to changes in ring currents when changing amino acid 48 from a tryptophan to a phenylalanine, ... average size of the head-group region of the bilayer. Only effects that result in a remaining amplitude of <0.4 are shown. Blue color indicates the effect of Mn 2+ ions, light red the effect of 1-palmitoyl-2-stearoyl-sn-glycero-5-doxyl-3-phosphatidylcholine,...

Ngày tải lên: 23/03/2014, 18:20

... E. (1994) Binding sites of quinones in photosynthetic bacterial reaction centers investigated by light- induced FTIR difference spectroscopy: binding of chainless symmetrical quinones to the Q A site of ... (1994) Binding sites of quinones in photosynthetic bacterial reaction centers investigated by light- induced FTIR difference spectroscopy: assignment of the interactions of each carbonyl of Q A in Rhodobacter ... stretching vibration of Q A by  60 cm )1 [10,11], indicating strong asymmetric binding of UQ 10 at the Q A site, in contrast to symmetric, weaker binding of UQ 10 at the Q B site [12,13]. To explain...

Ngày tải lên: 23/03/2014, 21:20

... identifi- cation of two light- dependent protein kinases involved in phosphorylation of thylakoid proteins. STN7 pro- tein kinase is essential for phosphorylation of Lhcb1, Lhcb2 and Lhcb4 proteins [11,17] ... conditions, including the process of leaf senes- cence in Arabidopsis [31]. The C-terminus of CaS contains also a motif for interaction with 14-3-3 proteins and FHA domains, according to eukaryotic linear ... motif that is involved in interaction with 14-3-3 proteins and proteins with the ‘forkhead-associated’ (FHA) domain. These domains are found in a variety of signaling pro- teins, and can bind directly...

Ngày tải lên: 30/03/2014, 04:20

Ngày tải lên: 30/03/2014, 10:20

Ngày tải lên: 18/06/2014, 19:20